LOCUS       NM_001360484            3182 bp    mRNA    linear   ROD 03-SEP-2024
DEFINITION  Mus musculus nudix hydrolase 15 (Nudt15), transcript variant 1,
            mRNA.
ACCESSION   NM_001360484 XM_017315941
VERSION     NM_001360484.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Wang,J., Zhang,Y., Li,L., Wang,L., Sun,S., Wang,B., Ge,Y. and
            Zhang,Z.
  TITLE     Nudt15-mediated inflammatory signaling contributes to divergent
            outcomes in leukemogenesis and hematopoiesis
  JOURNAL   Leukemia 38 (9), 1958-1970 (2024)
   PUBMED   39025986
  REMARK    GeneRIF: Nudt15-mediated inflammatory signaling contributes to
            divergent outcomes in leukemogenesis and hematopoiesis.
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Yamashita,N., Kawahara,M., Imai,T., Tatsumi,G., Asai-Nishishita,A.
            and Andoh,A.
  TITLE     Loss of Nudt15 thiopurine detoxification increases direct DNA
            damage in hematopoietic stem cells
  JOURNAL   Sci Rep 13 (1), 11908 (2023)
   PUBMED   37488179
  REMARK    GeneRIF: Loss of Nudt15 thiopurine detoxification increases direct
            DNA damage in hematopoietic stem cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3182)
  AUTHORS   Imai,T., Kawahara,M., Tatsumi,G., Yamashita,N., Nishishita-Asai,A.,
            Inatomi,O., Masamune,A., Kakuta,Y. and Andoh,A.
  TITLE     Thiopurine Use During Pregnancy Has Deleterious Effects on
            Offspring in Nudt15R138C Knock-In Mice
  JOURNAL   Cell Mol Gastroenterol Hepatol 12 (1), 335-337 (2021)
   PUBMED   33766784
  REMARK    Review article
REFERENCE   4  (bases 1 to 3182)
  AUTHORS   Tatsumi,G., Kawahara,M., Imai,T., Nishishita-Asai,A., Nishida,A.,
            Inatomi,O., Yokoyama,A., Kakuta,Y., Kito,K. and Andoh,A.
  TITLE     Thiopurine-mediated impairment of hematopoietic stem and leukemia
            cells in Nudt15R138C knock-in mice
  JOURNAL   Leukemia 34 (3), 882-894 (2020)
   PUBMED   31645647
  REMARK    GeneRIF: Thiopurine-mediated impairment of hematopoietic stem and
            leukemia cells in Nudt15(R138C) knock-in mice.
REFERENCE   5  (bases 1 to 3182)
  AUTHORS   Zheng,J.D., Hei,A.L., Zuo,P.P., Dong,Y.L., Song,X.N., Takagi,Y.,
            Sekiguchi,M. and Cai,J.P.
  TITLE     Age-related alterations in the expression of MTH2 in the
            hippocampus of the SAMP8 mouse with learning and memory
            deterioration
  JOURNAL   J Neurol Sci 287 (1-2), 188-196 (2009)
   PUBMED   19735921
  REMARK    GeneRIF: These results suggest that the MTH2 deficiency might be
            one of the causative factors for accelerated aging.
REFERENCE   6  (bases 1 to 3182)
  AUTHORS   Lau,N.C., Seto,A.G., Kim,J., Kuramochi-Miyagawa,S., Nakano,T.,
            Bartel,D.P. and Kingston,R.E.
  TITLE     Characterization of the piRNA complex from rat testes
  JOURNAL   Science 313 (5785), 363-367 (2006)
   PUBMED   16778019
REFERENCE   7  (bases 1 to 3182)
  AUTHORS   Cai,J.P., Ishibashi,T., Takagi,Y., Hayakawa,H. and Sekiguchi,M.
  TITLE     Mouse MTH2 protein which prevents mutations caused by 8-oxoguanine
            nucleotides
  JOURNAL   Biochem Biophys Res Commun 305 (4), 1073-1077 (2003)
   PUBMED   12767940
  REMARK    GeneRIF: MTH2 has a potential to protect the genetic material from
            the untoward effects of endogenous oxygen radicals; MTH2 could act
            as an MTH1 redundancy factor [MTH2]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY267802.1, AK040749.1, AK032657.1, AC113198.15 and AK043196.1.
            
            On Feb 6, 2018 this sequence version replaced XM_017315941.1.
            
            Transcript Variant: This variant (1) represents the predominant,
            but shorter, transcript. It has a transcriptional start site that
            is internal relative to that of variant 2. Variants 1 and 2 encode
            the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK043196.1, AK168451.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BY267802.1         2-16
            16-2197             AK040749.1         413-2594
            2198-2525           AK032657.1         2187-2514
            2526-2751           AC113198.15        52564-52789
            2752-3182           AK043196.1         2648-3078
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 39.06 cM"
     gene            1..3182
                     /gene="Nudt15"
                     /gene_synonym="6530403O17; A730068G11Rik; MTH2"
                     /note="nudix hydrolase 15"
                     /db_xref="GeneID:214254"
                     /db_xref="MGI:MGI:2443366"
     exon            1..190
                     /gene="Nudt15"
                     /gene_synonym="6530403O17; A730068G11Rik; MTH2"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..548
                     /gene="Nudt15"
                     /gene_synonym="6530403O17; A730068G11Rik; MTH2"
                     /EC_number="3.6.1.9"
                     /note="MutT homolog 2; probable 7,8-dihydro-8-oxoguanine
                     triphosphatase NUDT15; nudix motif 15; 8-oxo-dGTPase
                     NUDT15; nucleoside diphosphate-linked moiety X motif 15;
                     nudix (nucleoside diphosphate linked moiety X)-type motif
                     15; 7,8-dihydro-8-oxoguanine-triphosphatase NUDT15;
                     probable 8-oxo-dGTP diphosphatase NUDT15; nucleoside
                     diphosphate-linked to another moiety X hydrolase 15"
                     /codon_start=1
                     /product="nucleotide triphosphate diphosphatase NUDT15"
                     /protein_id="NP_001347413.1"
                     /db_xref="CCDS:CCDS27270.1"
                     /db_xref="GeneID:214254"
                     /db_xref="MGI:MGI:2443366"
                     /translation="MAANAEPRRRPGVGVGVVVLSCEHPRCVLLGKRKGSFGAGSFQL
                     PGGHLEFGETWEECAQRETWEEAGLHLKNVCFASVVNSFVEKENYHYVTILMKGEVDM
                     THDSEPRNMEPEKNESWEWVPWEEFPPLDQLFWALRCLKEQGYDPFKEDLNHLEGYRG
                     EHLERTTKTP"
     exon            191..387
                     /gene="Nudt15"
                     /gene_synonym="6530403O17; A730068G11Rik; MTH2"
                     /inference="alignment:Splign:2.1.0"
     exon            388..3182
                     /gene="Nudt15"
                     /gene_synonym="6530403O17; A730068G11Rik; MTH2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2486..2491
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nudt15"
                     /gene_synonym="6530403O17; A730068G11Rik; MTH2"
                     /note="hexamer: AATAAA"
     polyA_site      2526
                     /gene="Nudt15"
                     /gene_synonym="6530403O17; A730068G11Rik; MTH2"
                     /note="major polyA site"
     regulatory      3160..3165
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nudt15"
                     /gene_synonym="6530403O17; A730068G11Rik; MTH2"
                     /note="hexamer: AATAAA"
     polyA_site      3180
                     /gene="Nudt15"
                     /gene_synonym="6530403O17; A730068G11Rik; MTH2"
ORIGIN      
        1 agcttcaacg tcacttccgg ggtcagccaa atgctatggc cgctaacgca gagccgaggc
       61 ggcggccagg agttggggtc ggcgtggtgg tgctgagctg cgagcatcct cgctgcgtcc
      121 ttctggggaa aaggaaagga tcgtttggag ccggcagttt ccagcttcct gggggccatt
      181 tggaattcgg tgagacctgg gaagaatgcg ctcagagaga aacctgggaa gaagctgggc
      241 ttcacctgaa aaatgtgtgc tttgcctccg tggtaaattc ttttgttgag aaggagaatt
      301 accattatgt taccatatta atgaaaggag aggtggatat gactcatgat tcggagccga
      361 ggaatatgga gcctgaaaag aatgaaagtt gggagtgggt tccatgggaa gaattccctc
      421 ccttagacca gcttttctgg gctctccgct gtctaaaaga gcaaggttat gacccattta
      481 aagaggacct gaaccacctg gaagggtacc gaggagagca ccttgaaagg accacgaaga
      541 ctccttgatt aaaaacaaag tcttcaggaa atgaaaactt ctgtttcagg acaactcgat
      601 tttatccata gagccacgtg gtcaccagtt tatctgcagt ctcctgatat aactcaagaa
      661 ccttaagaaa ctcttcctgg ggtgtgccgc tgaattgtcc tggtggcata gaataaggtg
      721 tgtaaggagc ttgcagttca ttatctgcac ttctgcatgg cttccgctgt ccgctgcatg
      781 gaggcgagcg taatagattg ccttgtgcta gttcaaacag aggtcggaac tcaaatatgg
      841 ccattcaagt tcaaaagcaa ggcgcctact gccaagttca tcaagcttga tgttttcctt
      901 ttccctttca gccacttgaa aagaactttt aaatgacaat tcatgtaact agagagctta
      961 tgaaaagcag taggctacat tcccctggtg tctgagagac cccgtgtaag tgtgagagta
     1021 agctgtggct cccgccacta acggccaggt cctctgcatc tccctttcac agcctgagac
     1081 tggggtcctt gtgtgctggg atcacacagg gcaatgcatc gaatcaccta ctgggggcag
     1141 gggggtcccc agaggtcagg aactccagaa catcacagcc aaaggttggt tggctgcttt
     1201 ctccccctag caaattgtaa gcttttactg taagagattg aacagtgaaa tgtctccgag
     1261 gactgcattg tgtgctgcct gctgtcaaag cagagggtgt tctggcttga gagaggactt
     1321 tgggtttaag agaatcgtgt tcttggcctg aggaccgcat cgtgcttagc taacgttaag
     1381 agctaaagct tctgaccagc ccattcatgc cgatcagcag ccaggcttgg gttgcatgga
     1441 ccctcatctt tctctggtca ggcacagcat agctttctgc tgatgtagtt tctgttactg
     1501 agcagtgact ggcccttctg ctgtgcaggc ccctgctgag ctcttcatgt gaggtggttt
     1561 ctcaccagtg ccatctctgt cctgcacagg tgccatgtgg gtcctttact tgtggttcct
     1621 gttctttacc cagtattcct gtttcacatg ggtcaatttt tgatcagggc aaaaacatca
     1681 aagatagttg gagagtgctg gccgcacctc cggtgcacag cccaggcaga tggttgatgt
     1741 cagctgtgcc tgcacagccc agttttttat aatggccaga tatcactaat ttcttcattt
     1801 aatctcatat atctcaggct ggcttccagg tcactttgta gctaaggata accttgaact
     1861 tccagtcttg cctcagctgc cctgatgctg atttaagcta catcgcccac ccctctctaa
     1921 gcgtgcacag tagttgcccg tgtgtggctg gttagtttcc tgggttactt tgtttcagta
     1981 cttagggttg aacttggggc ttggcacatg acaggcaagt gccataacag actaaatgca
     2041 gaggcagtta gagaagccag cttccttctg tcaggtcaga cattgaagag atttgccaaa
     2101 atctaaaatg actttctcac tgaagagttc ataaaaacta tgttaacatg tacctggttt
     2161 ttactatatg gtcaataaac ttccctaact ttaatttcta acattaaata ttatttcata
     2221 gcttacaaaa ctaaaacctg actttaaggt tttataaagg catccaaacc taaggggacc
     2281 tggacattta gctcagtgct aggccacctg ctacgctccg ttcagtcctc atctccaaga
     2341 taaaaaaaat tacagaaaga ctaagaaaaa aatggcaccc aaaagggttc cgagtgcatt
     2401 agcccacatc tcctctttca taagctcgtt tctgtgaact gtctgacatc tctgaacatt
     2461 tgtagttttc tcttttaaaa cactgaataa atgtgatgtt tgctagtttt caacttttca
     2521 aaattacaat gtgctgtata tctatgtctc atctcttata cctacatatt tgtcaaaatg
     2581 tttgcattct gataatgata atgtatggtc cttaagcttc tgggtctagg gaggtggttc
     2641 atttgttaag agtgcttgct gcttgtcctt agaacccagc atcaacacca gctggcctag
     2701 tactcctaag acatcacctg cctctgccac acaggtaccg ggcatgtgcc accatgcctg
     2761 gaacatgtgg gatttaagta agaatctatt tcagaatgtt aacgggtact caagagtaga
     2821 tggccaggtt cactttgtgg tttatgctgt ttcttaaatg ttctgtgata gttattatat
     2881 aactatctaa aactgatact tatataaaca tcgtggacac agatgttaac tacgaggtag
     2941 ctaaagagtt aaaagtgact gttttttgag aaactacagt aagaggccaa ggcaggtggc
     3001 tgcaagtccc agactaacct aggtacctta tacaagcagg gagacccagt cctgtaaaag
     3061 atgagaaaag tcacaaggag acaaggtgat ggcatgagat ggccatctat tctaactttc
     3121 cttatcctgt gtgtatgtgt ctgtgtagta tggggttgta ataaatgttt tcagtttcaa
     3181 ct
//
