LOCUS       NM_175294               6096 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus nuclear casein kinase and cyclin-dependent kinase
            substrate 1 (Nucks1), transcript variant 1, mRNA.
ACCESSION   NM_175294
VERSION     NM_175294.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Yu,J., Zhang,H., Zhao,C., Li,G., Zhang,Y. and Sun,Y.
  TITLE     CircRNA circ_0008037 facilitates tumor growth and the Warburg
            effect via upregulating NUCKS1 by binding to miR-433-3p in
            non-small cell lung cancer
  JOURNAL   Thorac Cancer 13 (2), 162-172 (2022)
   PUBMED   34850570
  REMARK    GeneRIF: CircRNA circ_0008037 facilitates tumor growth and the
            Warburg effect via upregulating NUCKS1 by binding to miR-433-3p in
            non-small cell lung cancer.
REFERENCE   3  (bases 1 to 6096)
  AUTHORS   Zhang,Y., Chiu,S., Liang,X., Chai,Y.H., Qin,Y., Wang,J., Li,X.,
            Qiu,B., Tergaonkar,V., Tse,H.F. and Lian,Q.
  TITLE     Absence of NUCKS augments paracrine effects of mesenchymal stem
            cells-mediated cardiac protection
  JOURNAL   Exp Cell Res 356 (1), 74-84 (2017)
   PUBMED   28412246
  REMARK    GeneRIF: This study demonstrates that depletion of NUCKS enhances
            the therapeutic efficacy of Bone marrow-derived mesenchymal stem
            cells for myocardial infarction via regulating the secretion of
            VEGFa.
REFERENCE   4  (bases 1 to 6096)
  AUTHORS   Poon,M.W., Jiang,D., Qin,P., Zhang,Y., Qiu,B., Chanda,S.,
            Tergaonkar,V., Li,Q., Wong,I.Y., Yu,Z., Tse,H.F., Wong,D.S. and
            Lian,Q.
  TITLE     Inhibition of NUCKS Facilitates Corneal Recovery Following Alkali
            Burn
  JOURNAL   Sci Rep 7, 41224 (2017)
   PUBMED   28106169
  REMARK    GeneRIF: Study describes the role of NUCKS in corneal wound
            healing, inflammation and angiogenesis and links NUCKS to the
            activation of NF-kappa B and modulation of various cytokines.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6096)
  AUTHORS   Yue,Y., Leung,S.G., Liu,Y., Huang,Y., Grundt,K., Ostvold,A.C.,
            Jen,K.Y., Schild,D., Mao,J.H. and Wiese,C.
  TITLE     Nucks1 synergizes with Trp53 to promote radiation lymphomagenesis
            in mice
  JOURNAL   Oncotarget 7 (38), 61874-61889 (2016)
   PUBMED   27542204
  REMARK    GeneRIF: in the context of Trp53 deficiency, wild type levels of
            Nucks1 are required to suppress radiation-induced thymic lymphoma,
            likely through the role of the NUCKS1 protein in the DNA damage
            response
REFERENCE   6  (bases 1 to 6096)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   7  (bases 1 to 6096)
  AUTHORS   Shu,H., Chen,S., Bi,Q., Mumby,M. and Brekken,D.L.
  TITLE     Identification of phosphoproteins and their phosphorylation sites
            in the WEHI-231 B lymphoma cell line
  JOURNAL   Mol Cell Proteomics 3 (3), 279-286 (2004)
   PUBMED   14729942
REFERENCE   8  (bases 1 to 6096)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 6096)
  AUTHORS   Ostvold,A.C., Norum,J.H., Mathiesen,S., Wanvik,B., Sefland,I. and
            Grundt,K.
  TITLE     Molecular cloning of a mammalian nuclear phosphoprotein NUCKS,
            which serves as a substrate for Cdk1 in vivo
  JOURNAL   Eur J Biochem 268 (8), 2430-2440 (2001)
   PUBMED   11298763
REFERENCE   10 (bases 1 to 6096)
  AUTHORS   Ostvold,A.C., Holtlund,J. and Laland,S.G.
  TITLE     A novel, highly phosphorylated protein, of the high-mobility group
            type, present in a variety of proliferating and non-proliferating
            mammalian cells
  JOURNAL   Eur J Biochem 153 (3), 469-475 (1985)
   PUBMED   3000779
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ075701.1, AB049825.1, AC161805.4 and AC115001.11.
            
            On Mar 11, 2009 this sequence version replaced NM_175294.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.881.1, SRR10223971.777.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-66                CJ075701.1         15-80
            67-1206             AB049825.1         46-1185
            1207-2454           AC161805.4         1-1248              c
            2455-6096           AC115001.11        9865-13506          c
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 57.22 cM"
     gene            1..6096
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /note="nuclear casein kinase and cyclin-dependent kinase
                     substrate 1"
                     /db_xref="GeneID:98415"
                     /db_xref="MGI:MGI:1934811"
     exon            1..293
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /inference="alignment:Splign:2.1.0"
     CDS             277..981
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /note="isoform 1 is encoded by transcript variant 1;
                     nuclear ubiquitous casein and cyclin-dependent kinases
                     substrate; JC7; nuclear, casein kinase and
                     cyclin-dependent kinase substrate; nuclear ubiquitous
                     casein and cyclin-dependent kinase substrate 1"
                     /codon_start=1
                     /product="nuclear ubiquitous casein and cyclin-dependent
                     kinase substrate 1 isoform 1"
                     /protein_id="NP_780503.2"
                     /db_xref="CCDS:CCDS48356.1"
                     /db_xref="GeneID:98415"
                     /db_xref="MGI:MGI:1934811"
                     /translation="MSRPVRNRKVVDYSQFQESDDADEDYGRDSGPPAKKIRSSPREA
                     KNKRRSGKNSQEDSEDSEEKDVKTKKDDSHSAEDSEDEKDDHKNVRQQRQAASKAASK
                     QREMLLEDVGSEEEPEEDDEAPFQEKDSGSDEDFLMEDDDDSDYGSSKKKNKKMVKKS
                     KPERKEKKMPKPRLKATVTPSPVKGKAKVGRPTASKKSKEKTPSPKEEDEEAESPPEK
                     KSGDEGSEDEASSGED"
     exon            294..343
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /inference="alignment:Splign:2.1.0"
     exon            344..449
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /inference="alignment:Splign:2.1.0"
     exon            450..505
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /inference="alignment:Splign:2.1.0"
     exon            506..658
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /inference="alignment:Splign:2.1.0"
     exon            659..808
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /inference="alignment:Splign:2.1.0"
     exon            809..6096
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2097..2102
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /note="hexamer: AATAAA"
     polyA_site      2133
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /note="major polyA site"
     regulatory      2736..2741
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /note="hexamer: AATAAA"
     polyA_site      2745
                     /gene="Nucks1"
                     /gene_synonym="2700010L10Rik; 8430423A01Rik; Nucks"
                     /note="major polyA site"
ORIGIN      
        1 ggggcggagc gcacaaaccc tacggggttt ggttgcaccg cggctttggc gcattttcgg
       61 ctggtttgat tcatccattt tgaagagacg ggggagcggg gggctcgtca gatccaggga
      121 cctcaaacca gcagccgccg actcttgaga agccagcagc ccggggctcg gcagcagcgg
      181 ccccgccggc cggagcgggg gggtggggcg ccgagtgcgc ggggaggggg gtcctggtcc
      241 tcggctcctc gacttggtcg cgcctcgaca gcgaacatgt ctcggcctgt cagaaatagg
      301 aaggttgtgg attactctca gtttcaggaa tcagatgatg cagatgaaga ttatggaaga
      361 gattcaggcc ctcctgctaa gaagattcgg tcatctcccc gagaggctaa aaataagagg
      421 cgatctggaa aaaattcaca agaagacagt gaagactcag aagaaaaaga tgtgaagacc
      481 aagaaggatg attctcactc ggcagaagac agcgaagatg aaaaagatga tcataaaaat
      541 gtacgccagc aacgacaggc agcatctaaa gcagcttcta agcagagaga gatgctcttg
      601 gaagatgttg gcagcgagga agagccggaa gaagatgatg aggcgccatt ccaggagaaa
      661 gattctggca gcgatgaaga tttcctaatg gaagacgacg atgatagtga ttatggcagt
      721 tcaaaaaaga aaaacaaaaa gatggttaag aagtccaaac ctgagagaaa agaaaagaaa
      781 atgcccaaac ccagactaaa ggcaacagtg acgccaagtc cagtgaaagg caaagcgaaa
      841 gtgggtcgcc ccacagcttc gaagaaatca aaggaaaaaa ctccttctcc caaagaagag
      901 gatgaggaag cagaaagccc tccagaaaag aagtccggag acgaagggtc cgaggatgaa
      961 gcctcatctg gggaagatta aaagtgatgt ttagggagag gttttattaa aaacaaagga
     1021 aacctctgta ggacacggtt tgggctgtgg cttgactcat gggctttcag tgctatttca
     1081 tttgtctaga ataatgtttt tctctctatc atatatctct ttccctctta aaaccattgg
     1141 gttttttgtt tgtttgggtt ttttttttgt ttgtttgttt ggtttttgtt gttgttgggt
     1201 ttttttggtt tttttttttt ttcaagatag ggtttctctg tgtagccctg gctgtcctgg
     1261 aactcacttt gtagaccagg ctggcctcga actcagaaat ctgcctgcct ctgcctcccg
     1321 agctctggga ttaaaggggt gcaccaccac cgcctggctc ttaaaaccat tgtatgctta
     1381 cgtgtttaag ttgccatttt gtctcagtct tttctttgcc aatcccctcc ccccacgaaa
     1441 gccatgtcaa tcaatcactg gagtgactgt ttgattttcg agttgtcaat ggagggaagg
     1501 aatgccatgg ctgcaaagca ataagatttc aggtggacgc tatggaaact gcttcttgct
     1561 agagaacagc aacttgaaca ataatcaaaa acaaaaaact agaaaggttt taattaaata
     1621 tttgttcttt cttttcctgt acttttgaca gaccagttgt cacttaacat gagtttatat
     1681 gtctgaactg taagtgtcaa aaagaatcat gactctaaat tagtactagt gaggcagttg
     1741 gggtgaacat gaattctgga ttaactcagc actcacatta tttccttggg tgtggaggga
     1801 atggtgttcc tggaacttag tggatttgtt attctcaaag agattcagag ccagtgcttc
     1861 ttttgtcttg ataacggtct tttttctttt ttttttttta agtaggccac cagaatcagt
     1921 cttaatttca tattttattc atgttttcat tgattcaagt ttttaaaata cttcctgttt
     1981 ttgggtagtt tcaccaatga tccaaaatta aaaagagatt tctttgaatt tcgatgatga
     2041 atttacccct cacagtgcat ttcttcacag gctgacaatg ttaaaagttt taagcaaata
     2101 aagtttgagt tagtgtgaag ctcagatata aaagattgag atttatcctt tatgaataca
     2161 aataattgaa atgagggccc atgaaccatg ccacagttgt ttcctttcga tatttaaatc
     2221 tttcctccat agtaacatga taatgacata atctcacaac ctcctcctgc tgcttccata
     2281 ctgtggagtt gtttgcttct tagttggtct gacccctcct ttctgtttat gcttcttttt
     2341 aaagcaagag cctgagcctc aatgggatga atgaactgta ttttatttag ggtgcctctg
     2401 tgccattgat aacagtctag ataccttagc aaagcatcaa gatcaggttt gatcctccac
     2461 attcaggtcc cactcgccaa tagaagaaaa gattaggggt cctattatgc tattaaaaat
     2521 ttagtggagt aatttctgta tatttttctg cacttaagct aaatttgctt cctcagtatt
     2581 ctgcctttgt gttttttaag tgacatgttt gtctcagaca gtgtcatttt cttataggtg
     2641 ttcataggac aaagggttat ttaagaaata tttttactct ggtttttaag acactttaaa
     2701 gaacattctt agatctgctt taaaataaat aaatgaataa ataaagcacc tagtagcaga
     2761 gagttatttc aaccctgcac tgctaataaa tgagtattaa catggccaaa aatagaaact
     2821 tgaacataag cagttggatt attgtattct gtaatgggat ataaggttgt gagctatgta
     2881 gttggtatta cagattattc tatttgctgt ggtgggcttg ctcaggaacc cgtccttaaa
     2941 gaaagaagag actcaccatt tccaccaggc atctctccct tttaggggtt tataactcag
     3001 tcttacattc ctagtgacat ttaggctttt cttaggttat gtttcatgaa aagttgtggg
     3061 tagggtctct ttcaacttta ggcctcaatt ttttaacaat ctctttaata atctgcacta
     3121 acatctctga ttttatctac ctataattga gaaataatac tatcttctac gttacttgtg
     3181 taaaaggtgt gtctgattgg acttgtttag aattcctttt aaaactaact cattagattg
     3241 aaggtatatt ttctaattac agttcagttg ggttaatggt gtctcaaatg agcacacagg
     3301 ctttaagtga cctttgtttt cacttttttt tttagtggtt tataacactc tggtcttccc
     3361 ttcaaatcgt gtatgcaaac aggaacacat ttaatgtgac tcaactggaa accggtgctc
     3421 agcagttagg tgtaacaatg atatgagggt gtgcccctca gttcataatg actggccctt
     3481 ggcacctcta tggcttactc tccttcctct tttcctctgt ctttttcttt ctggtgtgcc
     3541 tatagtgaag tggcaatgta ttcaactgtc ttactttgca gtgctcagga atgggatgct
     3601 catttgaagg tgcttgtctg ttactgatgc tcagctaggt ttacctcttt agtagatgca
     3661 gcagttgagc taacactaca ttctagttct ctaggctctg gttaaagcct ttgtctcccc
     3721 ctgtcagaaa tggtagcctc tgtgttgccc agtggtaagt tactgctgct tttaaccgtc
     3781 caccactgat ggggttttgg agagaggacc caaagggtgt atttacatat ctgctgaaaa
     3841 acttaactgc tttcagtacc tccctctcca tattaacctt acaaaggaaa actaatggta
     3901 gcatgaatat ttcttttttt ctagggtagc cagagaatct ccacagacac cttaccaaaa
     3961 ccccagtatt ttgtgtaccc caggctggcc tcaaactcct gttttgtttt gtttttttca
     4021 aaacaactga tttttttttt cctgtcttca atgcttatat atggtactag ttcagatagc
     4081 attttcttcc acataaaaat cagtggaatt tgaggttata atctagaagg acttacagtc
     4141 agctgatctt gacctttttt gagcctggtt tcactttgta gtgtgtctgg gagttttttt
     4201 ccccccaccc ctcactcaca ctgctgggat taatgccacc atgtcaggtg acacttttta
     4261 aatgagcttt tatttttatg tccaggaaca tgtgggttag agagggaaat agtgtaaaca
     4321 aaactcaggt ttaggggccc ctgccagagc agacttgaag aaacagaaag ctttgttgct
     4381 gctgtgaatc cgtgcctctt tggacagttc tctctaccag tagcaccaga agctacacta
     4441 gaaacattcc cacctgtgac tgggtaggaa gaaatgcagg ctaggcactt catggtgtgc
     4501 ggacggaggt cagaaactag cagtacttgc ttcttgccaa aggggagggg aagttaaagc
     4561 cactggttgc tcttgagggt agaatattgt cattttctct atggaggaag ctgctgagtt
     4621 acttgaagat ggggaaggtt tttttgtttt gttttgtttt tttagttttt ccttttgttt
     4681 tttgagtatt gcgatgtcaa gctatcaata gccatgttac tatttgaacc tccttttttt
     4741 tccccctttg tcttagaaaa gagaatacat gattctgaat atcaaatcca taggttatac
     4801 ttttgttcaa agcctaattc acagtggtgt gattgtacta accactagga ggatcccata
     4861 tgcctacctg ttgccatgcc aatgaagaat gaactggtga catctgagca tgctctgtgg
     4921 ctgtagagca tcacagttgg gtggacactt tgtatattgg gtgtgggggg ttttctagat
     4981 catgggggag ggtaaaatga ttttgggtgg ggttttttgg tggttttttt tttccagttt
     5041 tgggggtttt ttgttttgtt tttgcttttt atggagggtt ggggagtata gtactcagtt
     5101 atgccctaaa aataacatgt ataaaaccct gaagtgtgat gtgggtatgt gtgagtgcgt
     5161 gtttgtaaat gtctggcatt taaacctttg tcttctggaa gttagtgaat tctttccctt
     5221 ttcctctgga agtatttgtt acaagatttg taaataagag ctctacatag tctgtgtacc
     5281 gtgaacatat tacagcaaac cttccatgct tcattcctct gcaaggcata agactgccag
     5341 gttgctcagc tcccgtgatc agtttgcctt cattcatctt ttagacttaa tttgacagtt
     5401 ctaaaaactc ctagtcttcc tcaattttag tctttaatct ttcatgttct gagcagaaag
     5461 ggtcagaaag ttagcctgtc ttcggccatg gctgagagcc tggcatcctt ttcttcatac
     5521 agtagtgtgg cttgatacat ggctagacaa cagagtgttg ggcattcttg caatacctta
     5581 gcagtgctga aatttgcagc atggtactaa ggaagctaaa gtttgaatgt aaccacttta
     5641 tttaaagggt tttttcttta atttaaatga taccgagttg aagtgaacat gattttgttg
     5701 accatgttca tgaattatag atgcaacatg cattggtagg ctcatgtgat ggtctgatgt
     5761 gatacttcat ttcatccatt tacataatcc tggtctgtac gcatttgcat aaacaacaac
     5821 aaactcctgc cttgctctga ttgaaggatt tccaggactg ctgtctgctc ctgaactttg
     5881 ttttaagtat gcgtatcctt tgcttgtatt ttgtattaag aaataaggaa aagaacccct
     5941 tattgttgag catgttggta ttgtcccctt tatttttttt ctcttgggga cattatgaag
     6001 caagttattc tttttctgta tctttttctt ttcttttgta aaccttttgg tttgttttgt
     6061 ttaaaaatgg ctttataaaa gggcttttgt aaccca
//
