LOCUS       NM_016736               3293 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus negative regulator of ubiquitin-like proteins 1
            (Nub1), transcript variant 2, mRNA.
ACCESSION   NM_016736
VERSION     NM_016736.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Brockmann,F., Catone,N., Wunsch,C., Offensperger,F., Scheffner,M.,
            Schmidtke,G. and Aichem,A.
  TITLE     FAT10 and NUB1L cooperate to activate the 26S proteasome
  JOURNAL   Life Sci Alliance 6 (8), e202201463 (2023)
   PUBMED   37188463
  REMARK    GeneRIF: FAT10 and NUB1L cooperate to activate the 26S proteasome.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3293)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3293)
  AUTHORS   Tanji,K., Miki,Y., Maruyama,A., Mori,F., Mimura,J., Itoh,K.,
            Kamitani,T. and Wakabayashi,K.
  TITLE     The role of NUB1 in alpha-synuclein degradation in Lewy body
            disease model mice
  JOURNAL   Biochem Biophys Res Commun 470 (3), 635-642 (2016)
   PUBMED   26797281
  REMARK    GeneRIF: These results suggest that increased levels of NUB1 play a
            potential role in degradation of detergent-insoluble
            alpha-synuclein in vivo, although it is insufficient to degrade
            abnormal alpha-synuclein in Lewy body disease model mice.
REFERENCE   5  (bases 1 to 3293)
  AUTHORS   Li,J., Ma,W., Li,H., Hou,N., Wang,X., Kim,I.M., Li,F. and Su,H.
  TITLE     NEDD8 Ultimate Buster 1 Long (NUB1L) Protein Suppresses Atypical
            Neddylation and Promotes the Proteasomal Degradation of Misfolded
            Proteins
  JOURNAL   J Biol Chem 290 (39), 23850-23862 (2015)
   PUBMED   26260793
  REMARK    GeneRIF: NUB1L suppresses atypical neddylation and promotes the
            degradation of misfolded proteins by the proteasome
REFERENCE   6  (bases 1 to 3293)
  AUTHORS   Tanaka,T., Kawashima,H., Yeh,E.T. and Kamitani,T.
  TITLE     Regulation of the NEDD8 conjugation system by a splicing variant,
            NUB1L
  JOURNAL   J Biol Chem 278 (35), 32905-32913 (2003)
   PUBMED   12816948
  REMARK    GeneRIF: NUB1 has a splicing variant, NUB1L, which regulates the
            NEDD8 conjugation system
REFERENCE   7  (bases 1 to 3293)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 3293)
  AUTHORS   Akey,D.T., Zhu,X., Dyer,M., Li,A., Sorensen,A., Blackshaw,S.,
            Fukuda-Kamitani,T., Daiger,S.P., Craft,C.M., Kamitani,T. and
            Sohocki,M.M.
  TITLE     The inherited blindness associated protein AIPL1 interacts with the
            cell cycle regulator protein NUB1
  JOURNAL   Hum Mol Genet 11 (22), 2723-2733 (2002)
   PUBMED   12374762
  REMARK    Erratum:[Hum Mol Genet. 2003 Feb 15;12(4):451]
REFERENCE   9  (bases 1 to 3293)
  AUTHORS   Kamitani,T., Kito,K., Fukuda-Kamitani,T. and Yeh,E.T.
  TITLE     Targeting of NEDD8 and its conjugates for proteasomal degradation
            by NUB1
  JOURNAL   J Biol Chem 276 (49), 46655-46660 (2001)
   PUBMED   11585840
REFERENCE   10 (bases 1 to 3293)
  AUTHORS   Kito,K., Yeh,E.T. and Kamitani,T.
  TITLE     NUB1, a NEDD8-interacting protein, is induced by interferon and
            down-regulates the NEDD8 expression
  JOURNAL   J Biol Chem 276 (23), 20603-20609 (2001)
   PUBMED   11259415
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK086301.1, AK134581.1, AC137938.12 and AI853857.1.
            
            On Mar 6, 2015 this sequence version replaced NM_016736.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and uses
            an alternate splice site in the 5' coding region, which results in
            use of a downstream start codon compared to variant 1. The encoded
            isoform (2) has a shorter N-terminus than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134581.1, SRR7345562.792099.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-169               AK086301.1         2-170
            170-3099            AK134581.1         2-2931
            3100-3101           AC137938.12        24949-24950
            3102-3274           AK134581.1         2935-3107
            3275-3293           AI853857.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 11.93 cM"
     gene            1..3293
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /note="negative regulator of ubiquitin-like proteins 1"
                     /db_xref="GeneID:53312"
                     /db_xref="MGI:MGI:1889001"
     exon            1..208
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            209..339
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..2067
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /note="isoform 2 is encoded by transcript variant 2; NEDD8
                     ultimate buster 1; NY-REN-18 antigen"
                     /codon_start=1
                     /product="NEDD8 ultimate buster 1 isoform 2"
                     /protein_id="NP_058016.2"
                     /db_xref="CCDS:CCDS39030.1"
                     /db_xref="GeneID:53312"
                     /db_xref="MGI:MGI:1889001"
                     /translation="MAQKKYLQAKLTQFLREDRIQLWKPPYTKENKEVGLAVKDLAKK
                     YSERLECCENEVENIIEEIRRKAIERGTGNEHYRTTGIATIEVFLPPRLRKHDKKSLL
                     ETRLHVTGRDLRCQIAETFGFQENYIKIVINKKQLQLGKSLEEQGVTHNVKAMVLELK
                     QSEEDVRKNLQLEEEEQNEAELKERRIQRTKRGLEILAERAEMVVDPETMPYLDIANQ
                     TGRSLRIPPAERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADRYFCECKELLDTV
                     DNYAVLQLDIVWCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKE
                     KVLFLRLYLLQGIQNYHSGNGEEAREYLNKARQLFKELYIDPSKVHNLLQLGFTAQEA
                     RLGLRACDGNVDHAATHISNRREELAQIRKEEKEKRRRRLENVNTLRGMGYSTQAAKQ
                     ALHQARGNLDDALKVLLSNPHMWWLQDADPENNSRQASPSQESINQLVYMGFDTVVAE
                     AALRVFGGNVQLAAQTLAHHGGSLPPDLQFSGEDSSPTPSTSPSDSAGTSSASTDEDM
                     ETEAVNEILEDIPEHEEDYLDSTLEDEEVIIAEYLSYVESISSAAKNN"
     exon            340..507
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            508..569
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            570..640
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            641..823
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            824..915
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1022
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1206
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1314
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1467
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1614
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1710
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1888
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..3278
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2093..2098
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /note="hexamer: TATAAA"
     polyA_site      2113
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /note="major polyA site"
     regulatory      2745..2750
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /note="hexamer: ATTAAA"
     polyA_site      2771
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /note="major polyA site"
     regulatory      3254..3259
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /note="hexamer: AATAAA"
     polyA_site      3278
                     /gene="Nub1"
                     /gene_synonym="4931404D21Rik; 6330412F12Rik; BS4;
                     NY-REN-18"
                     /note="major polyA site"
ORIGIN      
        1 agggcttcct tccgttcgat gcttcagatc gcccttcaag ccccttggcc ccacccttcc
       61 atgcagctcc ggtcctcgac cggccttccg gacagcgcga gggggcggag ccgggggcgg
      121 ggccggcctc acccggaatg aaaacaaacg gcggcctttg ccgcatccgg gcactcggca
      181 ggtcgcagct ggctcggcag tggcgcagtt ttactttcaa ggatggcgca gaagaaatat
      241 cttcaagcaa aactgactca gtttctaagg gaagacagaa ttcaactttg gaaacctcca
      301 tatactaaag aaaataagga agttggtttg gccgtaaagg accttgctaa gaagtactct
      361 gagcggctgg agtgctgtga aaacgaagtg gaaaacataa tcgaggaaat ccgacgcaaa
      421 gcaattgagc gcggcactgg gaatgaacac taccgaacaa ctgggatcgc caccattgag
      481 gtgttcctgc ctccaaggct ccggaagcac gataagaaaa gcttgttgga gacccgcttg
      541 catgtcactg gtagagacct gaggtgtcaa atagctgaaa ctttcggatt tcaagaaaat
      601 tacatcaaga tcgtaataaa taagaaacag cttcaactag ggaaaagcct tgaagaacaa
      661 ggagtcactc acaatgtgaa ggcgatggta ctggagctga aacagtctga agaggatgtg
      721 aggaaaaact tacagctaga ggaagaggaa cagaacgagg ccgagctgaa ggagagacgc
      781 attcagagga ccaaacgggg actggagatc ctggcggaga gagctgagat ggtggttgat
      841 ccggaaacga tgccttactt agacattgcg aaccagacag gcaggtcact cagaattccc
      901 cctgcagaaa gaaaagccct tatgttagct atgggatatc acgagaaggg cagagctttc
      961 ctgaaaagaa aagagtatgg aatagccctg ccctgcctcc tggacgctga cagatacttc
     1021 tgtgagtgca aagagctgct ggacactgtg gacaactatg cagtcctcca gctggacatt
     1081 gtgtggtgtt acttccgcct ggaacagctg gaatgccttg atgatgcaga gaaaaagctg
     1141 aacttggccc agaaatgctt taaaaactgt tacggagaga atcaccagag actggtccac
     1201 ataaaaggaa attgtgggaa agagaaggtg ttgtttttaa gactctactt gcttcaggga
     1261 atccaaaact atcacagtgg aaatggagag gaagctcggg aatatctcaa caaggcacgg
     1321 cagctcttta aggagcttta catcgatcca tcaaaagttc acaacttgtt acagttggga
     1381 ttcactgccc aggaagcacg gctgggccta cgggcctgcg atggaaacgt ggaccatgca
     1441 gccactcaca tttccaaccg cagagaggaa ctggcccaaa tccggaagga agagaaggag
     1501 aaaaggagac gccgtctgga aaatgtcaac actttgagag gaatgggcta ctccacacaa
     1561 gctgccaaac aagcccttca ccaggccaga ggcaacctgg acgatgccct gaaggttctc
     1621 ctcagcaacc cccacatgtg gtggttacag gatgcagacc ctgaaaacaa tagccgccaa
     1681 gcaagtcctt cccaggaaag catcaaccag ctggtgtaca tgggctttga cacagtggtg
     1741 gctgaagctg cactaagggt gtttggaggc aatgtccagc tggcagctca gacccttgca
     1801 caccatggag gaagcctccc acccgacctg cagttctcag gagaggactc ctcccccaca
     1861 ccgtccacat ccccatctga ctctgcaggg acctctagtg cctcgacaga tgaagacatg
     1921 gagacggagg ctgtcaacga aatcctggag gacattccgg agcacgagga ggactacctg
     1981 gactccacgc tggaggatga agaagtcatt attgctgaat acttgtccta cgttgaaagt
     2041 ataagttctg ccgcaaagaa caactgatca gaaataagtg ctacgtttcg gctataaatt
     2101 ctgtggttgt aaatggttga atgcttcttt gcgttcctct tgcttttcaa cgatttttct
     2161 ctaagtaggc ttcctcctca ggtacaggga cccagtgtat tgaagagact ttgtcagtca
     2221 gaggacagtc taggaaaggg aatgtcccag cctagctctg ctgtgccccg tcctcctcta
     2281 ggcctgcatc ccagactgcc actctgttgg ttttccattc agtgtaacag ggaagcaggt
     2341 cccccacccc caccccccac ctcctgaggc agggtaggta gtcccggaga aggtctgcag
     2401 agggcctggg actgtctcct gtcgtcttct cagctagcca tcctgtgcac tttgcccact
     2461 gtcctgctgc tgaactgccc tcctccccca ccacctctgc tgtgcaagct tctctcaccc
     2521 ttgctgccct gcatcaccca ttgcatcttc attccaagtg ctgtggagcc cagcaatgac
     2581 ggacaggctg ccagcctccc tcccaccgtg gcaggcggtg cagatccctt gatcgaaaaa
     2641 tgacagtgtt ttatgctctc agatcagtaa aatctgtact tgaaattaag gttgcaaaat
     2701 cagtttatat ggattctaaa tttctcatac aaagtgaaaa tcttattaaa tggaaaaaat
     2761 ttaaatgcta accacagcaa gttattttta attaacatct ctagactgga agcttactga
     2821 tagccaaggg acctgagatt gtgatctgct ttctcaggac tctccctggg tttcctataa
     2881 ctgggtcaca gaagacttgc tgaagaaggg cctgtcagcc tttggttgct tagtcctggg
     2941 tgtggccagg cagatagcct tctagcaccc aggcatggta acagccagat gtggtgtggg
     3001 ccaagtgcca ctgtgttccc aagagctttt catttcagct gaagccttgg cagcagacca
     3061 agctggacaa gtatgattgt ctctagctat tttttttttt aattacacat ttgtgtgggg
     3121 gatagggcag acataccaca gcacgagtct ggggctcaga agacaatgtg tggactgaac
     3181 tcaagctggc cccgcctcct gatggtcttg ctggccccct tgctgtgctt tggaactgaa
     3241 tgatgctgat ggaaataaac atggggaacg cacttgaaaa aaaaaaaaaa aaa
//
