LOCUS       NM_001195025            3127 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus NUAK family, SNF1-like kinase, 2 (Nuak2), transcript
            variant 1, mRNA.
ACCESSION   NM_001195025
VERSION     NM_001195025.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Lin,R., Wu,W., Chen,H., Gao,H., Wu,X., Li,G., He,Q., Lu,H., Sun,M.
            and Liu,Z.
  TITLE     GPR65 promotes intestinal mucosal Th1 and Th17 cell differentiation
            and gut inflammation through downregulating NUAK2
  JOURNAL   Clin Transl Med 12 (3), e771 (2022)
   PUBMED   35343079
  REMARK    GeneRIF: GPR65 promotes intestinal mucosal Th1 and Th17 cell
            differentiation and gut inflammation through downregulating NUAK2.
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Queiroz,A.L., Lessard,S.J., Ouchida,A.T., Araujo,H.N.,
            Goncalves,D.A., Simoes Froes Guimaraes,D.S.P., Teodoro,B.G., So,K.,
            Espreafico,E.M., Hirshman,M.F., Alberici,L.C., Kettelhut,I.D.C.,
            Goodyear,L.J. and Silveira,L.R.
  TITLE     The MicroRNA miR-696 is regulated by SNARK and reduces
            mitochondrial activity in mouse skeletal muscle through Pgc1alpha
            inhibition
  JOURNAL   Mol Metab 51, 101226 (2021)
   PUBMED   33812060
  REMARK    GeneRIF: The MicroRNA miR-696 is regulated by SNARK and reduces
            mitochondrial activity in mouse skeletal muscle through Pgc1alpha
            inhibition.
REFERENCE   3  (bases 1 to 3127)
  AUTHORS   Mammarella,E., Zampieri,C., Panatta,E., Melino,G. and Amelio,I.
  TITLE     NUAK2 and RCan2 participate in the p53 mutant pro-tumorigenic
            network
  JOURNAL   Biol Direct 16 (1), 11 (2021)
   PUBMED   34348766
  REMARK    GeneRIF: NUAK2 and RCan2 participate in the p53 mutant
            pro-tumorigenic network.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3127)
  AUTHORS   Alves,C.R.R., MacDonald,T.L., Nigro,P., Pathak,P., Hirshman,M.F.,
            Goodyear,L.J. and Lessard,S.J.
  TITLE     Reduced sucrose nonfermenting AMPK-related kinase (SNARK) activity
            aggravates cancer-induced skeletal muscle wasting
  JOURNAL   Biomed Pharmacother 117, 109197 (2019)
   PUBMED   31387190
  REMARK    GeneRIF: A role for SNARK in the maintenance of skeletal muscle
            mass under cachexia conditions.
REFERENCE   5  (bases 1 to 3127)
  AUTHORS   Chu,Q., Yao,C., Qi,X., Stripp,B.R. and Tang,N.
  TITLE     STK11 is required for the normal program of ciliated cell
            differentiation in airways
  JOURNAL   Cell Discov 5, 36 (2019)
   PUBMED   31636950
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3127)
  AUTHORS   Koh,H.J., Toyoda,T., Fujii,N., Jung,M.M., Rathod,A.,
            Middelbeek,R.J., Lessard,S.J., Treebak,J.T., Tsuchihara,K.,
            Esumi,H., Richter,E.A., Wojtaszewski,J.F., Hirshman,M.F. and
            Goodyear,L.J.
  TITLE     Sucrose nonfermenting AMPK-related kinase (SNARK) mediates
            contraction-stimulated glucose transport in mouse skeletal muscle
  JOURNAL   Proc Natl Acad Sci U S A 107 (35), 15541-15546 (2010)
   PUBMED   20713714
  REMARK    GeneRIF: Data show that SNARK is activated by muscle contraction
            and is a unique mediator of contraction-stimulated glucose
            transport in skeletal muscle.
REFERENCE   7  (bases 1 to 3127)
  AUTHORS   Ichinoseki-Sekine,N., Naito,H., Tsuchihara,K., Kobayashi,I.,
            Ogura,Y., Kakigi,R., Kurosaka,M., Fujioka,R. and Esumi,H.
  TITLE     Provision of a voluntary exercise environment enhances running
            activity and prevents obesity in Snark-deficient mice
  JOURNAL   Am J Physiol Endocrinol Metab 296 (5), E1013-E1021 (2009)
   PUBMED   19276392
REFERENCE   8  (bases 1 to 3127)
  AUTHORS   Tsuchihara,K., Ogura,T., Fujioka,R., Fujii,S., Kuga,W., Saito,M.,
            Ochiya,T., Ochiai,A. and Esumi,H.
  TITLE     Susceptibility of Snark-deficient mice to azoxymethane-induced
            colorectal tumorigenesis and the formation of aberrant crypt foci
  JOURNAL   Cancer Sci 99 (4), 677-682 (2008)
   PUBMED   18307533
  REMARK    GeneRIF: Obesity was a risk factor for colorectal tumorigenesis in
            Snark(+/-) mice model.
REFERENCE   9  (bases 1 to 3127)
  AUTHORS   Hirano,M., Kiyonari,H., Inoue,A., Furushima,K., Murata,T., Suda,Y.
            and Aizawa,S.
  TITLE     A new serine/threonine protein kinase, Omphk1, essential to ventral
            body wall formation
  JOURNAL   Dev Dyn 235 (8), 2229-2237 (2006)
   PUBMED   16715502
REFERENCE   10 (bases 1 to 3127)
  AUTHORS   Sakamoto,K., McCarthy,A., Smith,D., Green,K.A., Grahame Hardie,D.,
            Ashworth,A. and Alessi,D.R.
  TITLE     Deficiency of LKB1 in skeletal muscle prevents AMPK activation and
            glucose uptake during contraction
  JOURNAL   EMBO J 24 (10), 1810-1820 (2005)
   PUBMED   15889149
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160545.8.
            
            On Jan 24, 2023 this sequence version replaced NM_001195025.1.
            
            Transcript Variant: This variant (A) represents the longer
            transcript and encodes the longer isoform (A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046833.1, AK034082.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164136
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-336               AC160545.8         115175-115510
            337-457             AC160545.8         123991-124111
            458-481             AC160545.8         125244-125267
            482-633             AC160545.8         126735-126886
            634-699             AC160545.8         127169-127234
            700-819             AC160545.8         128996-129115
            820-952             AC160545.8         129597-129729
            953-3127            AC160545.8         130359-132533
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 57.24 cM"
     gene            1..3127
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /note="NUAK family, SNF1-like kinase, 2"
                     /db_xref="GeneID:74137"
                     /db_xref="MGI:MGI:1921387"
     exon            1..336
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..2013
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /EC_number="2.7.11.1"
                     /note="isoform A is encoded by transcript variant 1;
                     SNF1/AMP-activated protein kinase; omphalocele kinase 2"
                     /codon_start=1
                     /product="NUAK family SNF1-like kinase 2 isoform A"
                     /protein_id="NP_001181954.1"
                     /db_xref="CCDS:CCDS56642.1"
                     /db_xref="GeneID:74137"
                     /db_xref="MGI:MGI:1921387"
                     /translation="MESVALLQRPSQAPSASALASESARPLADGLIKSPKPLMKKQAV
                     KRHHHKHNLRHRYEFLETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLLHIR
                     REIEIMSSLNHPHIIAIHEVGRSRLVTVFENSSKIVIVMEYASRGDLYDYISERPRLS
                     ERDARHFFRQIVSALHYCHQNGIVHRDLKLENILLDANGNIKIADFGLSNLYHKGKFL
                     QTFCGSPLYASPEIVNGKPYVGPEVDSWSLGVLLYILVHGTMPFDGQDHKTLVKQISN
                     GAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYTTGVGEQEALREGGH
                     PSGDFGRASMADWLRRSSRPLLENGAKVCSFFKQHVPGGGSTVPGLERQHSLKKSRKE
                     NDMAQNLQGDPAEDTSSRPGKSSLKLPKGILKKKSSTSSGEVQEDPQELRPVPDTPGQ
                     PVPAVSLLPRKGILKKSRQRESGYYSSPEPSESGELLDASDVFVSGDPVEQKSPQASG
                     LLLHRKGILKLNGKFSRTALEGTTPSTFGSLDQLASSHPAARPSRPSGAVSEDSILSS
                     ESFDQLDLPERLPETPLRGCVSVDNLRGLEQPPSEGLKRWWQESLGDSCFSLTDCQEV
                     TAAYRQALGICSKLS"
     exon            337..457
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /inference="alignment:Splign:2.1.0"
     exon            458..481
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /inference="alignment:Splign:2.1.0"
     exon            482..633
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /inference="alignment:Splign:2.1.0"
     exon            634..699
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /inference="alignment:Splign:2.1.0"
     exon            700..819
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /inference="alignment:Splign:2.1.0"
     exon            820..952
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /inference="alignment:Splign:2.1.0"
     exon            953..3127
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2897..2902
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /note="hexamer: ATTAAA"
     polyA_site      2925
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /note="major polyA site"
     regulatory      3104..3109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /note="hexamer: AATAAA"
     polyA_site      3127
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
ORIGIN      
        1 agcgtgctcg ggtgcggctg tgacctctga gcccgcggct cagcgcgcgc tgctactgct
       61 gcccgaccca ctccacctcg cggtccccgc accatggagt cggtggcctt actccagcgc
      121 ccgagccagg ctccctcggc ctccgccctg gcctcggaga gcgcccggcc gctggcggac
      181 gggctcatca agtcgcctaa acctctgatg aagaagcagg cggtgaagcg gcaccatcac
      241 aaacacaacc tgcggcaccg ctacgagttc ctggagacgc tgggcaaggg cacctacggg
      301 aaggtgaaga aggcacgaga gagctcgggg cgtctggtgg ccatcaagtc catcaggaaa
      361 gacaaaatca aagatgagca ggatctgctg cacatacgga gggagattga gatcatgtct
      421 tcactcaacc acccccacat cattgccatc catgaagtgg gcagatcacg cttagtgact
      481 gtgtttgaga atagcagcaa gattgtgatt gtcatggagt atgccagccg aggcgatctg
      541 tatgattaca tcagtgagcg gccacggctg agtgagcggg acgccaggca tttcttccga
      601 cagatcgtgt ctgccctgca ctactgccac cagaacggga tcgttcaccg agatctcaag
      661 ctggaaaaca tccttctaga tgccaatgga aacatcaaga ttgctgactt tggcctctcc
      721 aacctgtacc acaaaggcaa gttcctccag acgttctgtg ggagccctct ctacgcctcg
      781 cctgagatag tcaacgggaa gccctatgtg ggcccagagg tggacagctg gtctctgggc
      841 gttctcctgt acatcctggt gcatggcacc atgccctttg acgggcagga tcataaaaca
      901 ctggtgaagc aaatcagtaa cggggcttac cgtgagccgc ccaagccgtc cgatgcctgt
      961 ggcctgatcc ggtggctgtt aatggtgaac cccacccgtc gggccacact ggaggatgta
     1021 gccagtcatt ggtgggtcaa ctggggttac accaccggag tcggggaaca ggaagccctg
     1081 cgtgagggtg ggcaccctag tggtgacttt ggccgggcct ccatggcgga ctggttacgt
     1141 cgctcctcgc gccccctcct ggagaatgga gccaaggtgt gcagcttctt caagcagcac
     1201 gtgccgggag gtggaagcac tgtacctggg ctggagcggc aacattctct taagaagtcc
     1261 cgaaaggaga atgacatggc tcaaaatctg caaggtgacc cggctgagga tacctcttct
     1321 cgccctggca agagcagcct taagcttccg aaaggcattc tcaagaaaaa gtcctctacc
     1381 tcgtcagggg aggtacagga ggaccctcag gaactcagac cggtgcctga tactccaggg
     1441 cagcctgtcc ctgctgtatc cctgctccca aggaaaggca tccttaagaa gtctcgacag
     1501 cgtgaatctg gttactactc ctctccagag cccagcgagt ctggggaact cttagacgcc
     1561 agtgatgtgt ttgtgagtgg ggaccccgtg gagcagaagt ctccacaggc ttcagggctc
     1621 ctcctccacc gcaagggcat tctcaaactc aatggcaagt tctcccgcac agccttagaa
     1681 ggcactaccc ctagcacctt tggctccctg gaccaactgg cctcctccca tcctgcagcc
     1741 cggcccagcc gcccctcagg ggctgtgagt gaggacagca tcctgtcctc cgagtccttt
     1801 gaccaattgg acttgcctga acgtcttccc gaaaccccac tgaggggctg tgtgtctgtg
     1861 gacaacctga gggggcttga gcagcctccc tcagaaggtc tgaagcgatg gtggcaggaa
     1921 tccttggggg atagctgctt ttctctgaca gactgccaag aggtgactgc agcctacaga
     1981 caagccctag gaatctgctc aaagctcagc tgaggaaggg agatggtgcc ctagtatggg
     2041 gtaggctctg agagggtttg cagaggaacc ctgggtcgga ttcctccagt gaatagagta
     2101 catcaagggc tctacgtctg cagcctgact gaacctgaaa gatgagagaa atcgcattga
     2161 tgtggaaagg aatgggaacc cttgctgccc gagtgttata gtggggtggc ctgaaggtgc
     2221 ctacctcctt tgtgccatga gtgtcaccca tgacatttcc caccctgttc tctggctgca
     2281 ccttcacata agtttctgtt tccatcaacc accagggtta gaaccctgac ttcctgggag
     2341 gtaatgtgta gtgactgcca ttatttagag aggaaacagc ctctggtttc catctctgct
     2401 gctgtgcatc tcaaagacct gggaagactc ggaccgctgt ttgacttcat ctcaagggga
     2461 ccagatgccc ctggacccca tcttagatct cagagacttg aaccttgaag ctgttcctag
     2521 tacccagatg tggatggatg ctctgtttct caggccaacg ggacctagaa tgtgctgact
     2581 tatttatttt tttgtgattc tcacttctgt tttttggttt ttgtttgttt gtttgttttt
     2641 gtttttaagt gaattttgct gctttcaata atgtgaatgc tgtgttctgg ggaactccac
     2701 tgtgccactg aagtttatgt acagagaagt atttggcaat gatgtccctc tattcaaggg
     2761 gggtgggggc gtttttcaaa tgtatgtctt gagcactgtc tggattgagt ctccagtccc
     2821 ttcacaccca aggctggcca ccctccctca tcttcatctg tggccaaaaa aaaaaaaaaa
     2881 aaaaaaaaca accctcatta aaaccagcag tggtaactgg aaaaacagtc tcttgttcac
     2941 tttggggact tgggttcaca catctttaag ccttttgata tttgtgacca cagatgggac
     3001 acagattctg tttaatggaa atagggcaag ggggttagga ggtgcagctg ggtgggtcag
     3061 gatgctgttt aatgtgtgct ctcaatcccc aggaacgtaa aagaataaaa tcaatcaatc
     3121 aaacaaa
//
