LOCUS       NP_001181954             639 aa            linear   ROD 14-JUN-2024
DEFINITION  NUAK family SNF1-like kinase 2 isoform A [Mus musculus].
ACCESSION   NP_001181954
VERSION     NP_001181954.1
DBSOURCE    REFSEQ: accession NM_001195025.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 639)
  AUTHORS   Lin,R., Wu,W., Chen,H., Gao,H., Wu,X., Li,G., He,Q., Lu,H., Sun,M.
            and Liu,Z.
  TITLE     GPR65 promotes intestinal mucosal Th1 and Th17 cell differentiation
            and gut inflammation through downregulating NUAK2
  JOURNAL   Clin Transl Med 12 (3), e771 (2022)
   PUBMED   35343079
  REMARK    GeneRIF: GPR65 promotes intestinal mucosal Th1 and Th17 cell
            differentiation and gut inflammation through downregulating NUAK2.
REFERENCE   2  (residues 1 to 639)
  AUTHORS   Queiroz,A.L., Lessard,S.J., Ouchida,A.T., Araujo,H.N.,
            Goncalves,D.A., Simoes Froes Guimaraes,D.S.P., Teodoro,B.G., So,K.,
            Espreafico,E.M., Hirshman,M.F., Alberici,L.C., Kettelhut,I.D.C.,
            Goodyear,L.J. and Silveira,L.R.
  TITLE     The MicroRNA miR-696 is regulated by SNARK and reduces
            mitochondrial activity in mouse skeletal muscle through Pgc1alpha
            inhibition
  JOURNAL   Mol Metab 51, 101226 (2021)
   PUBMED   33812060
  REMARK    GeneRIF: The MicroRNA miR-696 is regulated by SNARK and reduces
            mitochondrial activity in mouse skeletal muscle through Pgc1alpha
            inhibition.
REFERENCE   3  (residues 1 to 639)
  AUTHORS   Mammarella,E., Zampieri,C., Panatta,E., Melino,G. and Amelio,I.
  TITLE     NUAK2 and RCan2 participate in the p53 mutant pro-tumorigenic
            network
  JOURNAL   Biol Direct 16 (1), 11 (2021)
   PUBMED   34348766
  REMARK    GeneRIF: NUAK2 and RCan2 participate in the p53 mutant
            pro-tumorigenic network.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 639)
  AUTHORS   Alves,C.R.R., MacDonald,T.L., Nigro,P., Pathak,P., Hirshman,M.F.,
            Goodyear,L.J. and Lessard,S.J.
  TITLE     Reduced sucrose nonfermenting AMPK-related kinase (SNARK) activity
            aggravates cancer-induced skeletal muscle wasting
  JOURNAL   Biomed Pharmacother 117, 109197 (2019)
   PUBMED   31387190
  REMARK    GeneRIF: A role for SNARK in the maintenance of skeletal muscle
            mass under cachexia conditions.
REFERENCE   5  (residues 1 to 639)
  AUTHORS   Chu,Q., Yao,C., Qi,X., Stripp,B.R. and Tang,N.
  TITLE     STK11 is required for the normal program of ciliated cell
            differentiation in airways
  JOURNAL   Cell Discov 5, 36 (2019)
   PUBMED   31636950
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 639)
  AUTHORS   Koh,H.J., Toyoda,T., Fujii,N., Jung,M.M., Rathod,A.,
            Middelbeek,R.J., Lessard,S.J., Treebak,J.T., Tsuchihara,K.,
            Esumi,H., Richter,E.A., Wojtaszewski,J.F., Hirshman,M.F. and
            Goodyear,L.J.
  TITLE     Sucrose nonfermenting AMPK-related kinase (SNARK) mediates
            contraction-stimulated glucose transport in mouse skeletal muscle
  JOURNAL   Proc Natl Acad Sci U S A 107 (35), 15541-15546 (2010)
   PUBMED   20713714
  REMARK    GeneRIF: Data show that SNARK is activated by muscle contraction
            and is a unique mediator of contraction-stimulated glucose
            transport in skeletal muscle.
REFERENCE   7  (residues 1 to 639)
  AUTHORS   Ichinoseki-Sekine,N., Naito,H., Tsuchihara,K., Kobayashi,I.,
            Ogura,Y., Kakigi,R., Kurosaka,M., Fujioka,R. and Esumi,H.
  TITLE     Provision of a voluntary exercise environment enhances running
            activity and prevents obesity in Snark-deficient mice
  JOURNAL   Am J Physiol Endocrinol Metab 296 (5), E1013-E1021 (2009)
   PUBMED   19276392
REFERENCE   8  (residues 1 to 639)
  AUTHORS   Tsuchihara,K., Ogura,T., Fujioka,R., Fujii,S., Kuga,W., Saito,M.,
            Ochiya,T., Ochiai,A. and Esumi,H.
  TITLE     Susceptibility of Snark-deficient mice to azoxymethane-induced
            colorectal tumorigenesis and the formation of aberrant crypt foci
  JOURNAL   Cancer Sci 99 (4), 677-682 (2008)
   PUBMED   18307533
  REMARK    GeneRIF: Obesity was a risk factor for colorectal tumorigenesis in
            Snark(+/-) mice model.
REFERENCE   9  (residues 1 to 639)
  AUTHORS   Hirano,M., Kiyonari,H., Inoue,A., Furushima,K., Murata,T., Suda,Y.
            and Aizawa,S.
  TITLE     A new serine/threonine protein kinase, Omphk1, essential to ventral
            body wall formation
  JOURNAL   Dev Dyn 235 (8), 2229-2237 (2006)
   PUBMED   16715502
REFERENCE   10 (residues 1 to 639)
  AUTHORS   Sakamoto,K., McCarthy,A., Smith,D., Green,K.A., Grahame Hardie,D.,
            Ashworth,A. and Alessi,D.R.
  TITLE     Deficiency of LKB1 in skeletal muscle prevents AMPK activation and
            glucose uptake during contraction
  JOURNAL   EMBO J 24 (10), 1810-1820 (2005)
   PUBMED   15889149
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160545.8.
            
            Transcript Variant: This variant (A) represents the longer
            transcript and encodes the longer isoform (A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046833.1, AK034082.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164136
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 57.24 cM"
     Protein         1..639
                     /product="NUAK family SNF1-like kinase 2 isoform A"
                     /EC_number="2.7.11.1"
                     /note="SNF1/AMP-activated protein kinase; omphalocele
                     kinase 2"
                     /calculated_mol_wt=70544
     Region          1..21
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BZN4.2)"
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:Q9H093; propagated from
                     UniProtKB/Swiss-Prot (Q8BZN4.2)"
     Region          53..315
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(63..66,69,71,83,85,117,141..144,187,191..192,194,
                     204..205)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271063"
     Site            220
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q9H093; propagated from
                     UniProtKB/Swiss-Prot (Q8BZN4.2)"
     Region          372..485
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BZN4.2)"
     Region          529..575
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BZN4.2)"
     Site            538
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H093; propagated from
                     UniProtKB/Swiss-Prot (Q8BZN4.2)"
     Site            559
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H093; propagated from
                     UniProtKB/Swiss-Prot (Q8BZN4.2)"
     Site            562
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H093; propagated from
                     UniProtKB/Swiss-Prot (Q8BZN4.2)"
     Site            588
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355,
                     ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8BZN4.2)"
     CDS             1..639
                     /gene="Nuak2"
                     /gene_synonym="1200013B22Rik; mKIAA0537; Omphk2; Snark"
                     /coded_by="NM_001195025.2:94..2013"
                     /note="isoform A is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS56642.1"
                     /db_xref="GeneID:74137"
                     /db_xref="MGI:MGI:1921387"
ORIGIN      
        1 mesvallqrp sqapsasala sesarpladg likspkplmk kqavkrhhhk hnlrhryefl
       61 etlgkgtygk vkkaressgr lvaiksirkd kikdeqdllh irreieimss lnhphiiaih
      121 evgrsrlvtv fensskiviv meyasrgdly dyiserprls erdarhffrq ivsalhychq
      181 ngivhrdlkl enilldangn ikiadfglsn lyhkgkflqt fcgsplyasp eivngkpyvg
      241 pevdswslgv llyilvhgtm pfdgqdhktl vkqisngayr eppkpsdacg lirwllmvnp
      301 trratledva shwwvnwgyt tgvgeqealr egghpsgdfg rasmadwlrr ssrpllenga
      361 kvcsffkqhv pgggstvpgl erqhslkksr kendmaqnlq gdpaedtssr pgksslklpk
      421 gilkkkssts sgevqedpqe lrpvpdtpgq pvpavsllpr kgilkksrqr esgyysspep
      481 sesgelldas dvfvsgdpve qkspqasgll lhrkgilkln gkfsrtaleg ttpstfgsld
      541 qlasshpaar psrpsgavse dsilssesfd qldlperlpe tplrgcvsvd nlrgleqpps
      601 eglkrwwqes lgdscfsltd cqevtaayrq algicskls
//
