LOCUS       NM_008743               1080 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1),
            transcript variant 1, mRNA.
ACCESSION   NM_008743
VERSION     NM_008743.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Marsden,C.G., Das,L., Nottoli,T.P., Kathe,S.D., Doublie,S.,
            Wallace,S.S. and Sweasy,J.B.
  TITLE     Mouse Embryonic Fibroblasts Isolated From Nthl1 D227Y Knockin Mice
            Exhibit Defective DNA Repair and Increased Genome Instability
  JOURNAL   DNA Repair (Amst) 109, 103247 (2022)
   PUBMED   34826736
  REMARK    GeneRIF: Mouse Embryonic Fibroblasts Isolated From Nthl1 D227Y
            Knockin Mice Exhibit Defective DNA Repair and Increased Genome
            Instability.
REFERENCE   3  (bases 1 to 1080)
  AUTHORS   Chimienti,G., Pesce,V., Fracasso,F., Russo,F., de Souza-Pinto,N.C.,
            Bohr,V.A. and Lezza,A.M.S.
  TITLE     Deletion of OGG1 Results in a Differential Signature of Oxidized
            Purine Base Damage in mtDNA Regions
  JOURNAL   Int J Mol Sci 20 (13), 3302 (2019)
   PUBMED   31284385
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1080)
  AUTHORS   Cooley,N., Elder,R.H. and Povey,A.C.
  TITLE     The effect of Msh2 knockdown on toxicity induced by
            tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide
            in base excision repair proficient and deficient cells
  JOURNAL   Biomed Res Int 2013, 152909 (2013)
   PUBMED   23984319
  REMARK    GeneRIF: Toxicity, induced by tert-butyl-hydroperoxide and
            potassium bromate, differs in base excision repair proficient (Mpg
            (+/+), Nth1 (+/+)) and deficient (Mpg (-/-), Nth1 (-/-)) mouse
            embryonic fibroblasts following Msh2 knockdown, was examined.
REFERENCE   5  (bases 1 to 1080)
  AUTHORS   Vallabhaneni,H., O'Callaghan,N., Sidorova,J. and Liu,Y.
  TITLE     Defective repair of oxidative base lesions by the DNA glycosylase
            Nth1 associates with multiple telomere defects
  JOURNAL   PLoS Genet 9 (7), e1003639 (2013)
   PUBMED   23874233
  REMARK    GeneRIF: These results suggest that Nth1 plays an important role in
            telomere maintenance and base repair against oxidative
            stress-induced base modifications.
REFERENCE   6  (bases 1 to 1080)
  AUTHORS   Ocampo,M.T., Chaung,W., Marenstein,D.R., Chan,M.K., Altamirano,A.,
            Basu,A.K., Boorstein,R.J., Cunningham,R.P. and Teebor,G.W.
  TITLE     Targeted deletion of mNth1 reveals a novel DNA repair enzyme
            activity
  JOURNAL   Mol Cell Biol 22 (17), 6111-6121 (2002)
   PUBMED   12167705
  REMARK    GeneRIF: To determine the role of murine Nth1 in protecting genomic
            integrity, a knockout strain was created. No phenotypic changes
            from wild type were noted due to a novel AP endonuclease DNA repair
            system.
REFERENCE   7  (bases 1 to 1080)
  AUTHORS   Takao,M., Kanno,S., Shiromoto,T., Hasegawa,R., Ide,H., Ikeda,S.,
            Sarker,A.H., Seki,S., Xing,J.Z., Le,X.C., Weinfeld,M.,
            Kobayashi,K., Miyazaki,J., Muijtjens,M., Hoeijmakers,J.H., van der
            Horst,G. and Yasui,A.
  TITLE     Novel nuclear and mitochondrial glycosylases revealed by disruption
            of the mouse Nth1 gene encoding an endonuclease III homolog for
            repair of thymine glycols
  JOURNAL   EMBO J 21 (13), 3486-3493 (2002)
   PUBMED   12093749
  REMARK    GeneRIF: To determine the significance of thymine glycol repair in
            mammals, a mouse model with mutated mNth1, a homolog of nth, was
            made by gene targeting. Mutant mouse liver, instead of mNTH1
            activity, had at least 2 novel DNA glycosylase activities against
            Tg.
            Erratum:[EMBO J 2002 Aug 15;21(16):4391. Sarker Altraf H [corrected
            to Sarker Altaf H]]
REFERENCE   8  (bases 1 to 1080)
  AUTHORS   Nomoto,M., Yamaguchi,R., Kohno,K. and Kasai,H.
  TITLE     Relations between clusters of oxidatively damaged nucleotides and
            active or open nucleosomes in the rat Nth 1 gene
  JOURNAL   Oncogene 21 (11), 1649-1657 (2002)
   PUBMED   11896596
REFERENCE   9  (bases 1 to 1080)
  AUTHORS   Luna,L., Bjoras,M., Hoff,E., Rognes,T. and Seeberg,E.
  TITLE     Cell-cycle regulation, intracellular sorting and induced
            overexpression of the human NTH1 DNA glycosylase involved in
            removal of formamidopyrimidine residues from DNA
  JOURNAL   Mutat Res 460 (2), 95-104 (2000)
   PUBMED   10882850
REFERENCE   10 (bases 1 to 1080)
  AUTHORS   Sarker,A.H., Ikeda,S., Nakano,H., Terato,H., Ide,H., Imai,K.,
            Akiyama,K., Tsutsui,K., Bo,Z., Kubo,K., Yamamoto,K., Yasui,A.,
            Yoshida,M.C. and Seki,S.
  TITLE     Cloning and characterization of a mouse homologue (mNthl1) of
            Escherichia coli endonuclease III
  JOURNAL   J Mol Biol 282 (4), 761-774 (1998)
   PUBMED   9743625
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132367.3, CX565655.1, AV377313.2 and CJ211561.1.
            
            On Apr 28, 2009 this sequence version replaced NM_008743.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y09688.1, AB006812.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AC132367.3         11783-11803         c
            22-723              CX565655.1         1-702
            724-1078            AV377313.2         312-666
            1079-1080           CJ211561.1         399-400
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 12.42 cM"
     gene            1..1080
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /note="nth (endonuclease III)-like 1 (E.coli)"
                     /db_xref="GeneID:18207"
                     /db_xref="MGI:MGI:1313275"
     exon            1..173
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..973
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /EC_number="4.2.99.18"
                     /note="isoform 1 is encoded by transcript variant 1;
                     endonuclease III-like protein 1; thymine glycol DNA
                     glycosylase/AP lyase; DNA glycoslyase/AP lyase;
                     bifunctional DNA N-glycoslyase/DNA-(apurinic or
                     apyrimidinic site) lyase; bifunctional DNA
                     N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /codon_start=1
                     /product="endonuclease III-like protein 1 isoform 1"
                     /protein_id="NP_032769.2"
                     /db_xref="CCDS:CCDS28487.1"
                     /db_xref="GeneID:18207"
                     /db_xref="MGI:MGI:1313275"
                     /translation="MNSGVRMVTRSRSRATRIASEGCREELAPREAAAEGRKSHRPVR
                     HPRRTQKTHVAYEAANGEEGEDAEPLKVPVWEPQNWQQQLANIRIMRSKKDAPVDQLG
                     AEHCYDASASPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVESILQTDDDTL
                     GRLIYPVGFWRNKVKYIKQTTAILQQRYEGDIPASVAELVALPGVGPKMAHLAMAVAW
                     GTISGIAVDTHVHRIANRLRWTKKMTKTPEETRKNLEEWLPRVLWSEVNGLLVGFGQQ
                     ICLPVHPRCQACLNKALCPAAQDL"
     exon            174..412
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /inference="alignment:Splign:2.1.0"
     exon            413..583
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /inference="alignment:Splign:2.1.0"
     exon            584..743
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /inference="alignment:Splign:2.1.0"
     exon            744..849
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /inference="alignment:Splign:2.1.0"
     exon            850..1080
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1052..1057
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /note="hexamer: AATAAA"
     polyA_site      1080
                     /gene="Nthl1"
                     /gene_synonym="Nth1; Octs3"
                     /note="major polyA site"
ORIGIN      
        1 agtttcgcgt gcgcgttgag caagtggtgg gatcgcgggg ctgctcggag gagtagttta
       61 cagcccaggc atgaactcag gggtgcggat ggtgactcgc agtcggagcc gcgcgactag
      121 gatcgcgtcg gaagggtgta gggaggagct cgccccgcga gaggctgctg cagaaggaag
      181 aaaaagccac aggcccgtga gacatccacg gagaacacag aaaacgcatg tggcctatga
      241 agcggctaat ggtgaggaag gcgaagatgc tgagcccctc aaagtgcccg tttgggagcc
      301 ccagaactgg cagcagcaac tggccaacat ccgcatcatg agaagcaaga aggatgcacc
      361 tgtggaccag ctaggcgccg agcactgcta tgatgcaagt gcctccccga aggtgaggag
      421 gtaccaggta ctcctgtcgc tgatgctctc cagccagacc aaagaccagg tcacagcagg
      481 tgctatgcaa cggctccggg cccggggctt gactgtggag agcatcctgc agaccgatga
      541 tgacacgcta ggcagactca tctaccctgt gggcttctgg aggaacaagg taaaatacat
      601 caagcagaca accgccatcc tgcagcagcg ctacgaaggg gacatccctg cttccgtggc
      661 tgagctggta gccttgccag gtgttgggcc caagatggca cacttggcta tggctgtggc
      721 ctgggggacc atatcaggca tagcagtgga cacacatgtg cacagaatag ccaaccgact
      781 gaggtggacc aagaagatga ccaagacccc agaagagaca cgtaagaact tggaagagtg
      841 gctacccagg gtgctgtgga gtgaggtcaa cggactactg gtaggcttcg gccaacagat
      901 ctgtcttcct gtccatcctc gatgtcaggc ttgcctcaac aaggccctgt gccctgctgc
      961 ccaggatctc taagggccat agggcttcct ctggctagtg tctgtgaggt gcctttgtgg
     1021 ggcggggtgg taaactttgg ggggtgtttg caataaacct gggaagtttt tcacagttga
//
