LOCUS       NM_010946               1189 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus N-terminal Asn amidase (Ntan1), transcript variant 1,
            mRNA.
ACCESSION   NM_010946
VERSION     NM_010946.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Furtado,G.C., Pacer,M.E., Bongers,G., Benezech,C., He,Z., Chen,L.,
            Berin,M.C., Kollias,G., Caamano,J.H. and Lira,S.A.
  TITLE     TNFalpha-dependent development of lymphoid tissue in the absence of
            RORgammat lymphoid tissue inducer cells
  JOURNAL   Mucosal Immunol 7 (3), 602-614 (2014)
   PUBMED   24129162
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Goto,Y., Taniura,H., Yamada,K., Hirai,T., Sanada,N., Nakamichi,N.
            and Yoneda,Y.
  TITLE     The magnetism responsive gene Ntan1 in mouse brain
  JOURNAL   Neurochem Int 49 (4), 334-341 (2006)
   PUBMED   16600435
  REMARK    GeneRIF: when embryonic 12-day-old or newborn mice were
            successively exposed to magnetic fields Ntan1 mRNA was
            significantly increased
REFERENCE   3  (bases 1 to 1189)
  AUTHORS   Balogh,S.A., McDowell,C.S. and Denenberg,V.H.
  TITLE     Behavioral characterization of mice lacking the ubiquitin ligase
            UBR1 of the N-end rule pathway
  JOURNAL   Genes Brain Behav 1 (4), 223-229 (2002)
   PUBMED   12882367
REFERENCE   4  (bases 1 to 1189)
  AUTHORS   Kwon,Y.T., Xia,Z., Davydov,I.V., Lecker,S.H. and Varshavsky,A.
  TITLE     Construction and analysis of mouse strains lacking the ubiquitin
            ligase UBR1 (E3alpha) of the N-end rule pathway
  JOURNAL   Mol Cell Biol 21 (23), 8007-8021 (2001)
   PUBMED   11689692
REFERENCE   5  (bases 1 to 1189)
  AUTHORS   Balogh,S.A., McDowell,C.S., Tae Kwon,Y. and Denenberg,V.H.
  TITLE     Facilitated stimulus-response associative learning and long-term
            memory in mice lacking the NTAN1 amidase of the N-end rule pathway
  JOURNAL   Brain Res 892 (2), 336-343 (2001)
   PUBMED   11172781
REFERENCE   6  (bases 1 to 1189)
  AUTHORS   Balogh,S.A., Kwon,Y.T. and Denenberg,V.H.
  TITLE     Varying intertrial interval reveals temporally defined memory
            deficits and enhancements in NTAN1-deficient mice
  JOURNAL   Learn Mem 7 (5), 279-286 (2000)
   PUBMED   11040259
REFERENCE   7  (bases 1 to 1189)
  AUTHORS   Kwon,Y.T., Balogh,S.A., Davydov,I.V., Kashina,A.S., Yoon,J.K.,
            Xie,Y., Gaur,A., Hyde,L., Denenberg,V.H. and Varshavsky,A.
  TITLE     Altered activity, social behavior, and spatial memory in mice
            lacking the NTAN1p amidase and the asparagine branch of the N-end
            rule pathway
  JOURNAL   Mol Cell Biol 20 (11), 4135-4148 (2000)
   PUBMED   10805755
REFERENCE   8  (bases 1 to 1189)
  AUTHORS   Puech,A., Saint-Jore,B., Funke,B., Gilbert,D.J., Sirotkin,H.,
            Copeland,N.G., Jenkins,N.A., Kucherlapati,R., Morrow,B. and
            Skoultchi,A.I.
  TITLE     Comparative mapping of the human 22q11 chromosomal region and the
            orthologous region in mice reveals complex changes in gene
            organization
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14608-14613 (1997)
   PUBMED   9405660
REFERENCE   9  (bases 1 to 1189)
  AUTHORS   Grigoryev,S., Stewart,A.E., Kwon,Y.T., Arfin,S.M., Bradshaw,R.A.,
            Jenkins,N.A., Copeland,N.G. and Varshavsky,A.
  TITLE     A mouse amidase specific for N-terminal asparagine. The gene, the
            enzyme, and their function in the N-end rule pathway
  JOURNAL   J Biol Chem 271 (45), 28521-28532 (1996)
   PUBMED   8910481
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC107769.7, BY254590.1,
            BY168976.1, AK041112.1 and AW492524.1.
            
            On Sep 28, 2016 this sequence version replaced NM_010946.3.
            
            Summary: The protein encoded by this gene functions in a step-wise
            protein degradation process through the N-end rule pathway. This
            protein acts as a tertiary destabilizing enzyme that deamidates
            N-terminal L-Asparagine residues on proteins to produce N-terminal
            L-Aspartate. L-Aspartate substrates are subsequently conjugated to
            L-Arginine, which is recognized by specific E3 ubiquitin ligases
            and targeted to the proteasome. Mice with a knock-out of this gene
            are viable, fertile, and outwardly normal, but show impairments in
            spontaneous activity and spatial memory, relative to their
            wild-type counterparts. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC030172.1, U57692.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AC107769.7         163396-163421       c
            27-64               BY254590.1         1-38
            65-187              BY168976.1         1-123
            188-929             AK041112.1         119-860
            930-1189            AW492524.1         1-260               c
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 9.64 cM"
     gene            1..1189
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /note="N-terminal Asn amidase"
                     /db_xref="GeneID:18203"
                     /db_xref="MGI:MGI:108471"
     exon            1..186
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..1038
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /EC_number="3.5.1.121"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein N-terminal asparagine amidohydrolase; protein
                     NTN-amidase; protein N-terminal Asn amidase; protein
                     NH2-terminal asparagine deamidase; protein NH2-terminal
                     asparagine amidohydrolase; asparagine-specific N-terminal
                     amidase"
                     /codon_start=1
                     /product="protein N-terminal asparagine amidohydrolase
                     isoform 1"
                     /protein_id="NP_035076.1"
                     /db_xref="CCDS:CCDS27969.1"
                     /db_xref="GeneID:18203"
                     /db_xref="MGI:MGI:108471"
                     /translation="MPLLVDGQRVRLPRSAVELVRAHPPLEERARLLRGQSVQQVGPQ
                     GLLYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGSDTKAEV
                     PLIMSSIKSFSEHAECGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDKQDDDIHLVTLC
                     VTELNDREENENHFPIIYGIAVNIKTAEIYRASFQDRGPEEQLRAARALAGGPMISIY
                     DAKTEQLRIGPCSWTPFPQVDFWLQQDDKQILESLSTSPLAEPPHFVEHIRSTLMFLK
                     KFPSPENILFPGNKALLYKKNKDGLWEKISSPGS"
     exon            187..289
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            290..355
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            356..464
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            465..538
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            539..592
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            593..646
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            647..744
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            745..858
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1174
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1148..1153
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /note="hexamer: AATAAA"
     polyA_site      1169
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /note="major polyA site"
ORIGIN      
        1 ggcgcggcgc ccacggtggg cgtggcatgc cccgaggcgg ggcgaagcgg agcggggcgg
       61 ggcggggcgg tggccaccta actcggcctg gcggcggcct agacgatgcc actgctggtg
      121 gatgggcagc gcgtccgtct gccacggtcc gctgtggagc tggtccgggc gcacccgcct
      181 ctggaggaaa gagccagact tctcagaggt cagtctgttc aacaagtggg accccagggc
      241 cttctgtatg ttcagcaaag agagcttgca gtgacctccc caaaggatgg ctccatctcc
      301 attctgggtt ctgatgatgc taccacttgt cacattgtag tcctgagaca tacaggaaat
      361 ggggcaacct gcctgacaca ttgtgatgga tctgacacca aagctgaagt ccccttgata
      421 atgagctcca taaaatcctt ctctgaacat gctgagtgtg gaaggctgga agtacatctt
      481 gtgggaggct tcagtgatga caggcagctg tcacagaaac ttactcatca gcttctcagt
      541 gaatttgaca aacaggatga tgacattcac ttagtgacat tatgtgtaac agagttaaat
      601 gaccgtgaag aaaatgaaaa tcactttcca atcatttatg gcatcgctgt caacattaaa
      661 actgcagaga tttacagagc ttcctttcaa gatcgtggtc cagaggagca actgcgtgct
      721 gcaagagctt tagcaggagg cccaatgatt agcatttatg atgcaaagac agaacaactc
      781 cgaataggcc cgtgttcctg gacgccattc ccacaagtgg atttctggtt gcagcaagat
      841 gacaagcaaa tcctagagag cctttccacg tctcctctgg ccgagccccc ccactttgtt
      901 gaacatatta gatctacctt gatgttttta aaaaaattcc catctccaga gaatatactc
      961 tttcctggaa ataaagctct cctctacaaa aaaaataaag atggcttgtg ggaaaagatc
     1021 tcctctccag ggagctaaca tcagctcaga gtttactgaa gaattcatct tcacctgata
     1081 aagatggttg ctggtttgga atctacacct gcacactttt acttcttccg tccttcctgt
     1141 ctttcaaaat aaaatcttat tttggcaaag gcaaaaaaaa aaaaaaaaa
//
