LOCUS       NM_001346111             906 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus N-terminal Asn amidase (Ntan1), transcript variant 7,
            mRNA.
ACCESSION   NM_001346111
VERSION     NM_001346111.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Furtado,G.C., Pacer,M.E., Bongers,G., Benezech,C., He,Z., Chen,L.,
            Berin,M.C., Kollias,G., Caamano,J.H. and Lira,S.A.
  TITLE     TNFalpha-dependent development of lymphoid tissue in the absence of
            RORgammat lymphoid tissue inducer cells
  JOURNAL   Mucosal Immunol 7 (3), 602-614 (2014)
   PUBMED   24129162
REFERENCE   2  (bases 1 to 906)
  AUTHORS   Goto,Y., Taniura,H., Yamada,K., Hirai,T., Sanada,N., Nakamichi,N.
            and Yoneda,Y.
  TITLE     The magnetism responsive gene Ntan1 in mouse brain
  JOURNAL   Neurochem Int 49 (4), 334-341 (2006)
   PUBMED   16600435
  REMARK    GeneRIF: when embryonic 12-day-old or newborn mice were
            successively exposed to magnetic fields Ntan1 mRNA was
            significantly increased
REFERENCE   3  (bases 1 to 906)
  AUTHORS   Balogh,S.A., McDowell,C.S. and Denenberg,V.H.
  TITLE     Behavioral characterization of mice lacking the ubiquitin ligase
            UBR1 of the N-end rule pathway
  JOURNAL   Genes Brain Behav 1 (4), 223-229 (2002)
   PUBMED   12882367
REFERENCE   4  (bases 1 to 906)
  AUTHORS   Kwon,Y.T., Xia,Z., Davydov,I.V., Lecker,S.H. and Varshavsky,A.
  TITLE     Construction and analysis of mouse strains lacking the ubiquitin
            ligase UBR1 (E3alpha) of the N-end rule pathway
  JOURNAL   Mol Cell Biol 21 (23), 8007-8021 (2001)
   PUBMED   11689692
REFERENCE   5  (bases 1 to 906)
  AUTHORS   Balogh,S.A., McDowell,C.S., Tae Kwon,Y. and Denenberg,V.H.
  TITLE     Facilitated stimulus-response associative learning and long-term
            memory in mice lacking the NTAN1 amidase of the N-end rule pathway
  JOURNAL   Brain Res 892 (2), 336-343 (2001)
   PUBMED   11172781
REFERENCE   6  (bases 1 to 906)
  AUTHORS   Balogh,S.A., Kwon,Y.T. and Denenberg,V.H.
  TITLE     Varying intertrial interval reveals temporally defined memory
            deficits and enhancements in NTAN1-deficient mice
  JOURNAL   Learn Mem 7 (5), 279-286 (2000)
   PUBMED   11040259
REFERENCE   7  (bases 1 to 906)
  AUTHORS   Kwon,Y.T., Balogh,S.A., Davydov,I.V., Kashina,A.S., Yoon,J.K.,
            Xie,Y., Gaur,A., Hyde,L., Denenberg,V.H. and Varshavsky,A.
  TITLE     Altered activity, social behavior, and spatial memory in mice
            lacking the NTAN1p amidase and the asparagine branch of the N-end
            rule pathway
  JOURNAL   Mol Cell Biol 20 (11), 4135-4148 (2000)
   PUBMED   10805755
REFERENCE   8  (bases 1 to 906)
  AUTHORS   Puech,A., Saint-Jore,B., Funke,B., Gilbert,D.J., Sirotkin,H.,
            Copeland,N.G., Jenkins,N.A., Kucherlapati,R., Morrow,B. and
            Skoultchi,A.I.
  TITLE     Comparative mapping of the human 22q11 chromosomal region and the
            orthologous region in mice reveals complex changes in gene
            organization
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14608-14613 (1997)
   PUBMED   9405660
REFERENCE   9  (bases 1 to 906)
  AUTHORS   Grigoryev,S., Stewart,A.E., Kwon,Y.T., Arfin,S.M., Bradshaw,R.A.,
            Jenkins,N.A., Copeland,N.G. and Varshavsky,A.
  TITLE     A mouse amidase specific for N-terminal asparagine. The gene, the
            enzyme, and their function in the N-end rule pathway
  JOURNAL   J Biol Chem 271 (45), 28521-28532 (1996)
   PUBMED   8910481
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC107769.7, BY254590.1,
            CJ057344.1, CK617520.1 and AW492524.1.
            
            Summary: The protein encoded by this gene functions in a step-wise
            protein degradation process through the N-end rule pathway. This
            protein acts as a tertiary destabilizing enzyme that deamidates
            N-terminal L-Asparagine residues on proteins to produce N-terminal
            L-Aspartate. L-Aspartate substrates are subsequently conjugated to
            L-Arginine, which is recognized by specific E3 ubiquitin ligases
            and targeted to the proteasome. Mice with a knock-out of this gene
            are viable, fertile, and outwardly normal, but show impairments in
            spontaneous activity and spatial memory, relative to their
            wild-type counterparts. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2016].
            
            Transcript Variant: This variant (7) differs in the 5' UTR, uses an
            alternate start codon, and lacks three alternate exons in the
            coding region, compared to variant 1. The encoded isoform (7) is
            shorter and has a distinct N-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.5014340.1,
                                           SRR7652917.265315.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AC107769.7         163396-163421       c
            27-76               BY254590.1         1-50
            77-77               AC107769.7         163345-163345       c
            78-105              BY254590.1         52-79
            106-187             CJ057344.1         39-120
            188-574             CK617520.1         183-569
            575-906             AW492524.1         1-332               c
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 9.64 cM"
     gene            1..906
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /note="N-terminal Asn amidase"
                     /db_xref="GeneID:18203"
                     /db_xref="MGI:MGI:108471"
     exon            1..186
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..755
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /EC_number="3.5.1.121"
                     /note="isoform 7 is encoded by transcript variant 7;
                     protein N-terminal asparagine amidohydrolase; protein
                     NTN-amidase; protein N-terminal Asn amidase; protein
                     NH2-terminal asparagine deamidase; protein NH2-terminal
                     asparagine amidohydrolase; asparagine-specific N-terminal
                     amidase"
                     /codon_start=1
                     /product="protein N-terminal asparagine amidohydrolase
                     isoform 7"
                     /protein_id="NP_001333040.1"
                     /db_xref="GeneID:18203"
                     /db_xref="MGI:MGI:108471"
                     /translation="MPRGGAKRSGAGRGGGHLTRPGGGLDDATAGGWAARPSATVRCG
                     AGPGAPASGGNGATCLTHCDGSDTKAEVPLIMSSIKSFSEHAECGRLEVHLVGGFSDD
                     RQLSQKLTHQLLSEFDKQDDDIHLVTLCVTELNDREENENHFPIIYGIAVNIKTAEIY
                     RASFQDRGPEEQLRAARALAGGPSLSTSPLAEPPHFVEHIRSTLMFLKKFPSPENILF
                     PGNKALLYKKNKDGLWEKISSPGS"
     exon            187..295
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            296..369
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            370..423
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            424..477
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            478..575
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     exon            576..891
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      865..870
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /note="hexamer: AATAAA"
     polyA_site      886
                     /gene="Ntan1"
                     /gene_synonym="PNAA; PNAD"
                     /note="major polyA site"
ORIGIN      
        1 ggcgcggcgc ccacggtggg cgtggcatgc cccgaggcgg ggcgaagcgg agcggggcgg
       61 ggcggggcgg tggccaccta actcggcctg gcggcggcct agacgatgcc actgctggtg
      121 gatgggcagc gcgtccgtct gccacggtcc gctgtggagc tggtccgggc gcacccgcct
      181 ctggaggaaa tggggcaacc tgcctgacac attgtgatgg atctgacacc aaagctgaag
      241 tccccttgat aatgagctcc ataaaatcct tctctgaaca tgctgagtgt ggaaggctgg
      301 aagtacatct tgtgggaggc ttcagtgatg acaggcagct gtcacagaaa cttactcatc
      361 agcttctcag tgaatttgac aaacaggatg atgacattca cttagtgaca ttatgtgtaa
      421 cagagttaaa tgaccgtgaa gaaaatgaaa atcactttcc aatcatttat ggcatcgctg
      481 tcaacattaa aactgcagag atttacagag cttcctttca agatcgtggt ccagaggagc
      541 aactgcgtgc tgcaagagct ttagcaggag gcccaagcct ttccacgtct cctctggccg
      601 agccccccca ctttgttgaa catattagat ctaccttgat gtttttaaaa aaattcccat
      661 ctccagagaa tatactcttt cctggaaata aagctctcct ctacaaaaaa aataaagatg
      721 gcttgtggga aaagatctcc tctccaggga gctaacatca gctcagagtt tactgaagaa
      781 ttcatcttca cctgataaag atggttgctg gtttggaatc tacacctgca cacttttact
      841 tcttccgtcc ttcctgtctt tcaaaataaa atcttatttt ggcaaaggca aaaaaaaaaa
      901 aaaaaa
//
