LOCUS       NM_011851               3580 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus 5' nucleotidase, ecto (Nt5e), mRNA.
ACCESSION   NM_011851
VERSION     NM_011851.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Serafin,P.K., Popeda,M., Bulak,K., Zwara,A., Galikowska-Bogut,B.,
            Przychodzka,A., Mika,A., Sledzinski,T., Stanislawowski,M.,
            Jendernalik,K., Bolcewicz,M., Laprus,W., Stasilojc,G., Sadej,R.,
            Zaczek,A., Kalinowski,L. and Koszalka,P.
  TITLE     Knock-out of CD73 delays the onset of HR-negative breast cancer by
            reprogramming lipid metabolism and is associated with increased
            tumor mutational burden
  JOURNAL   Mol Metab 89, 102035 (2024)
   PUBMED   39304062
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Zhao,N., Shao,Z., Xia,G., Liu,H., Zhang,L., Zhao,X., Dang,S.,
            Qian,L., Xu,W., Yu,Z. and Wang,R.
  TITLE     Protective role of the CD73-A2AR axis in cirrhotic cardiomyopathy
            through negative feedback regulation of the NF-kappaB pathway
  JOURNAL   Front Immunol 15, 1428551 (2024)
   PUBMED   39086479
  REMARK    GeneRIF: Protective role of the CD73-A2AR axis in cirrhotic
            cardiomyopathy through negative feedback regulation of the
            NF-kappaB pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3580)
  AUTHORS   Tan,Z., Chen,P., Dong,X., Guo,S., Leung,V.Y.L., Cheung,J.P.Y.,
            Chan,D., Richardson,S.M., Hoyland,J.A., To,M.K.T. and Cheah,K.S.E.
  TITLE     Progenitor-like cells contributing to cellular heterogeneity in the
            nucleus pulposus are lost in intervertebral disc degeneration
  JOURNAL   Cell Rep 43 (6), 114342 (2024)
   PUBMED   38865240
REFERENCE   4  (bases 1 to 3580)
  AUTHORS   Duan,Y., Chen,X., Shao,H., Li,Y., Zhang,Z., Li,H., Zhao,C.,
            Xiao,H., Wang,J. and Zhang,X.
  TITLE     Enhanced immunosuppressive capability of mesenchymal stem
            cell-derived small extracellular vesicles with high expression of
            CD73 in experimental autoimmune uveitis
  JOURNAL   Stem Cell Res Ther 15 (1), 149 (2024)
   PUBMED   38783393
  REMARK    GeneRIF: Enhanced immunosuppressive capability of mesenchymal stem
            cell-derived small extracellular vesicles with high expression of
            CD73 in experimental autoimmune uveitis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3580)
  AUTHORS   Eltzschig,H.K., Thompson,L.F., Karhausen,J., Cotta,R.J., Ibla,J.C.,
            Robson,S.C. and Colgan,S.P.
  TITLE     Endogenous adenosine produced during hypoxia attenuates neutrophil
            accumulation: coordination by extracellular nucleotide metabolism
  JOURNAL   Blood 104 (13), 3986-3992 (2004)
   PUBMED   15319286
REFERENCE   6  (bases 1 to 3580)
  AUTHORS   Tsuji,Y., Kaburagi,Y., Terauchi,Y., Satoh,S., Kubota,N.,
            Tamemoto,H., Kraemer,F.B., Sekihara,H., Aizawa,S., Akanuma,Y.,
            Tobe,K., Kimura,S. and Kadowaki,T.
  TITLE     Subcellular localization of insulin receptor substrate family
            proteins associated with phosphatidylinositol 3-kinase activity and
            alterations in lipolysis in primary mouse adipocytes from IRS-1
            null mice
  JOURNAL   Diabetes 50 (6), 1455-1463 (2001)
   PUBMED   11375348
REFERENCE   7  (bases 1 to 3580)
  AUTHORS   Braun,N., Brendel,P. and Zimmermann,H.
  TITLE     Distribution of 5'-nucleotidase in the developing mouse retina
  JOURNAL   Brain Res Dev Brain Res 88 (1), 79-86 (1995)
   PUBMED   7493409
REFERENCE   8  (bases 1 to 3580)
  AUTHORS   Resta,R., Hooker,S.W., Hansen,K.R., Laurent,A.B., Park,J.L.,
            Blackburn,M.R., Knudsen,T.B. and Thompson,L.F.
  TITLE     Murine ecto-5'-nucleotidase (CD73): cDNA cloning and tissue
            distribution
  JOURNAL   Gene 133 (2), 171-177 (1993)
   PUBMED   8224905
REFERENCE   9  (bases 1 to 3580)
  AUTHORS   Schoen,S.W. and Graybiel,A.M.
  TITLE     Species-specific patterns of glycoprotein expression in the
            developing rodent caudoputamen: association of 5'-nucleotidase
            activity with dopamine islands and striosomes in rat, but with
            extrastriosomal matrix in mouse
  JOURNAL   J Comp Neurol 333 (4), 578-596 (1993)
   PUBMED   8103780
REFERENCE   10 (bases 1 to 3580)
  AUTHORS   Berkemeier,L.R., Winslow,J.W., Kaplan,D.R., Nikolics,K.,
            Goeddel,D.V. and Rosenthal,A.
  TITLE     Neurotrophin-5: a novel neurotrophic factor that activates trk and
            trkB
  JOURNAL   Neuron 7 (5), 857-866 (1991)
   PUBMED   1742028
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB850214.1 and AK029979.1.
            
            On Mar 13, 2010 this sequence version replaced NM_011851.3.
            
            Summary: This gene encodes a membrane-bound nucleotidase that
            hydrolyzes extracellular nucleoside monophosphates. The encoded
            preproprotein undergoes proteolytic processing to generate to a
            functional, homodimeric enzyme that preferentially uses adenosine
            monophosphate as a substrate to generate free adenosine. Mice
            lacking the encoded protein exhibit a significantly reduced fall in
            stop flow pressure and superficial nephron glomerular filtration
            rate in response to a saturating increase of tubular perfusion
            flow. [provided by RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029979.1, AK154614.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                BB850214.1         2-32
            32-3580             AK029979.1         2-3550
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 47.24 cM"
     gene            1..3580
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /note="5' nucleotidase, ecto"
                     /db_xref="GeneID:23959"
                     /db_xref="MGI:MGI:99782"
     exon            1..422
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..1808
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.91"
                     /EC_number="3.1.3.89"
                     /EC_number="3.1.3.99"
                     /EC_number="3.1.3.35"
                     /note="5'-nucleotidase; ecto-5'-nucleotidase;
                     5'-deoxynucleotidase; thymidylate 5'-phosphatase;
                     IMP-specific 5'-nucleotidase"
                     /codon_start=1
                     /product="5'-nucleotidase preproprotein"
                     /protein_id="NP_035981.1"
                     /db_xref="CCDS:CCDS23386.1"
                     /db_xref="GeneID:23959"
                     /db_xref="MGI:MGI:99782"
                     /translation="MRPAAAKVPKWLLLALSALLPQWPAASAWELTILHTNDVHSRLE
                     QTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLE
                     VAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLF
                     LPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKII
                     ALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQ
                     VPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLD
                     NYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVN
                     GGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGE
                     FLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGD
                     GFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKFSAASHYQGSFPLVILS
                     FWAMILILYQ"
     exon            423..645
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            646..834
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            835..1032
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1187
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1293
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1443
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1644
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..3580
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3561..3566
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /note="hexamer: AATAAA"
     polyA_site      3578
                     /gene="Nt5e"
                     /gene_synonym="2210401F01Rik; 5'-NT; CD73; eNT; NT; Nt5"
                     /note="major polyA site"
ORIGIN      
        1 agtttagtag aggccccggt ggccggtgct cactgggctc agggccagtc cacccgctca
       61 acgcgctcta gccagccatg cgtcccgcgg ccgctaaggt acccaagtgg ctgcttctcg
      121 cactgagcgc tctactacca cagtggcccg cagccagtgc ctgggagctc acgatcctgc
      181 acacaaacga cgtgcacagc cggctagagc agaccagcga tgactccacc aagtgcctca
      241 acgccagcct gtgtgtgggc ggcgtggccc ggctctttac caaggtgcag cagatccgca
      301 aggaagaacc caacgtgctg tttttggatg ccggagacca gtaccagggc accatctggt
      361 tcaccgttta caaaggcctt gaagtggcac acttcatgaa catcctgggc tacgatgcta
      421 tggcactggg aaatcatgaa tttgataacg gtgtggaagg actgattgat cccctcctca
      481 gaaacgttaa atttccaatt ctgagcgcaa acattaaggc acgggggcct ctagcacatc
      541 agatatctgg actttttctg ccatctaaag ttctctctgt tggcggtgag gttgtgggga
      601 ttgttggata tacttcaaag gaaacccctt tcctctcaaa tccagggaca aatttagtct
      661 ttgaagatga aatctctgca ttgcagcctg aagtagataa actaaagacg ctaaatgtga
      721 ataagatcat cgccctgggg cactctggtt ttgagatgga caaacttatc gctcagaaag
      781 ttcgaggtgt ggacatcgtg gtgggaggac actccaacac ctttctctac acaggaaatc
      841 caccttccaa agaagtgcct gcggggaagt acccattcat agtcaccgca gatgatggac
      901 ggcaggtgcc tgtggtccag gcctatgcct ttggcaaata cctgggctac ctgaaggttg
      961 agtttgatga taaaggcaat gttatcactt cctatggaaa tcccattctt ctcaacagca
     1021 gcattcctga agatgcgacc atcaaagcag acattaacca atggaggata aaattagata
     1081 attattctac ccaggaactc gggagaacga tcgtctacct ggatggctcc actcagacgt
     1141 gccgcttcag ggaatgcaac atgggaaacc tgatctgtga tgccatgatt aacaacaacc
     1201 tcagacaccc agatgaaatg ttttggaacc acgtgtccat gtgcattgta aacggaggtg
     1261 gcatccggtc ccccattgat gagaagaaca atggtaccat cacctgggag aacctggctg
     1321 ctgtgctgcc ctttggaggg acatttgacc tcgtccaatt aaaagggtcc accctgaaga
     1381 aggcttttga gcacagcgtg catcgctatg gccagtccac cggagagttc ctgcaagtgg
     1441 gtggaatcca tgtggtgtac gatattaacc gaaagccctg gaacagagtg gtccaattag
     1501 aagttctctg caccaagtgt cgagtgccca tctatgagcc tcttgaaatg gataaagtgt
     1561 ataaagtgac cctcccaagc tatctggcca acggtggaga tggattccag atgataaaag
     1621 atgaattact aaagcatgac tctggtgatc aagatatcag cgtggtttct gaatacatct
     1681 caaaaatgaa agtagtttac ccagccgttg aagggcggat caagttctct gcagcaagtc
     1741 attaccaggg aagctttcct ttagtaattc tttcattttg ggcaatgatc cttattttgt
     1801 accaataaca gggagtctcc ttgtccttga tgtcaaacta catttttctt ccagtgatat
     1861 tcatatctgc ctctggagac ctggctttgt aacagcactc atcatcctca aggttcctag
     1921 cagatgttct tcacaaggaa gagactgtaa catcatttgt tggggccagc aactcagtga
     1981 gcagatagaa agtcacagtg aaccaacagg gtccttctgg cagggagtgg gtaaggggaa
     2041 acaactagat gtagcttgca tagccacata acacatctgg ttaccatttc cctttctatt
     2101 catttctaat ccatcaaaca attgatgttt acatacaact tcatcatcgc cagctctggt
     2161 ggcacatgcc tgtggtcacg gcacttggca gggaggagag gatggctgca agttctaggc
     2221 cagcctgaca tatgtagagt ttcaggccag tcagctagat atcaagactc acacacacaa
     2281 acaaacatta taatttacaa gtagattttt gtagacaagt cttatgataa gctaagcaga
     2341 aagggtcgac ttgttcaagg tcagacatct ccaaataata catctaagat ctgtacctga
     2401 gtctttttac ctcaagtcca acctctcgcc tactgtcaag tctcctctct tcctgcggtt
     2461 ggtctcagac aacaaatctg tttctgcctt ccatagtacc ctttcttttg ggctcttgtt
     2521 gtctctcagg tttgagagag tagctactgg acaggattct ttcctacaac accatgtgcc
     2581 tttggtgagt catagacaaa ctgtatacag cagataatag attagtccag ggactgcaaa
     2641 aggcagtcag ggacaggcag gaaagggaaa ggagaatatg accaggactt acagaagaga
     2701 gtagatgggt tccataaaca ctagttaagg agcagaacat ggctccaaag cacaacactg
     2761 gttcttatta actcctgtgc gactccaaaa gccctgcctc tctccttcct tcttcctcac
     2821 atggaggtgc catgagagaa cttcctaaaa taaactgtaa ttctcaacct gcacctgtcc
     2881 tgtccaagat gccaacacgg ctctcattca agtcacacat gtacaatcca atattctgga
     2941 aggcaagagt gaatttaaga agtggtatac ataaatgttt gaagtcactg agactcaaat
     3001 cccacacaac cactgtaagg catactcagg tcaagacatg agaagaccag caggacagtg
     3061 agccctatgg gaggcccaag agatacccag actgatgaaa tgatctgtgc ccacagaaca
     3121 ccagaccaag acagtcctgt gaccaagtga gcatagtcag cactgtacag tagttaagaa
     3181 tccttaacta taaaccagca gctagatgtc tggacaggga acatgttggt ttcctagtgt
     3241 ttacgaatat taaggactct tgacccaaac cgtttaaaat tccaatcctt ttatgaagtt
     3301 tgttgcagtg aaacaatgcc ttcagttctt tttgaatgtg tagattagtt ataaacagaa
     3361 atgtcagtaa gactgtaaaa aaaaatgaag aaaggtgcat atgaggtata tttttatgct
     3421 tggcgagtaa ataaggagaa atcttatagc ataattttta aagaacattt gtataatttt
     3481 tctaaatatg tgtatatgta tattttatgc agcagtattg agaaatgacc ttggactatc
     3541 ttgtaattgt aaattccaaa aataaagctg aagatatagt
//
