LOCUS       NM_001252374            1530 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus 5'-nucleotidase, cytosolic III (Nt5c3), transcript
            variant 2, mRNA.
ACCESSION   NM_001252374
VERSION     NM_001252374.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1530)
  AUTHORS   Ipata,P.L. and Balestri,F.
  TITLE     The functional logic of cytosolic 5'-nucleotidases
  JOURNAL   Curr Med Chem 20 (34), 4205-4216 (2013)
   PUBMED   23992316
  REMARK    Review article
REFERENCE   4  (bases 1 to 1530)
  AUTHORS   Magni,G., Amici,A. and Orsomando,G.
  TITLE     The enzymology of cytosolic pyrimidine 5'-nucleotidases: functional
            analysis and physiopathological implications
  JOURNAL   Curr Med Chem 20 (34), 4304-4316 (2013)
   PUBMED   23992312
  REMARK    Review article
REFERENCE   5  (bases 1 to 1530)
  AUTHORS   Grobosky,C.L., Lopez,J.B., Rennie,S., Skopelitis,D.J., Wiest,A.T.,
            Bingman,C.A. and Bitto,E.
  TITLE     Structural basis of substrate specificity and selectivity of murine
            cytosolic 5'-nucleotidase III
  JOURNAL   J Mol Biol 423 (4), 540-554 (2012)
   PUBMED   22925580
  REMARK    GeneRIF: report describes the first X-ray structure of a
            catalytically inactivated variant of murine cN-III with a natural
            substrate, uridine 5'-monophosphate, in the active site at 1.74A
            resolution
REFERENCE   6  (bases 1 to 1530)
  AUTHORS   Lu,M.M., Chen,F., Gitler,A., Li,J., Jin,F., Ma,X.K. and
            Epstein,J.A.
  TITLE     Cloning and expression analysis of murine lupin, a member of a
            novel gene family that is conserved through evolution and
            associated with Lupus inclusions
  JOURNAL   Dev Genes Evol 210 (10), 512-517 (2000)
   PUBMED   11180800
REFERENCE   7  (bases 1 to 1530)
  AUTHORS   Rich,S.A., Bose,M., Tempst,P. and Rudofsky,U.H.
  TITLE     Purification, microsequencing, and immunolocalization of p36, a new
            interferon-alpha-induced protein that is associated with human
            lupus inclusions
  JOURNAL   J Biol Chem 271 (2), 1118-1126 (1996)
   PUBMED   8557639
REFERENCE   8  (bases 1 to 1530)
  AUTHORS   Swallow,D.M., Turner,V.S. and Hopkinson,D.A.
  TITLE     Isozymes of rodent 5'-nucleotidase: evidence for two independent
            structural loci Umph-1 and Umph-2
  JOURNAL   Ann Hum Genet 47 (1), 9-17 (1983)
   PUBMED   6301357
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153616.8, AK160139.1, CX734167.1 and BX527194.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038029.1, AK160139.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AC153616.8         94157-94160
            5-375               AK160139.1         5-375
            376-698             CX734167.1         1-323               c
            699-1189            AK160139.1         700-1190
            1190-1530           BX527194.1         134-474
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 27.75 cM"
     gene            1..1530
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /note="5'-nucleotidase, cytosolic III"
                     /db_xref="GeneID:107569"
                     /db_xref="MGI:MGI:1927186"
     exon            1..49
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     exon            50..104
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..962
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.91"
                     /note="isoform 2 is encoded by transcript variant 2;
                     cytosolic 5'-nucleotidase 3; pyrimidine 5'-nucleotidase 1;
                     cytosolic 5'-nucleotidase III; 5'-nucleotidase,
                     pyrimidine; uridine monophosphatase 1; cytosolic
                     5'-nucleotidase 3A; 7-methylguanosine nucleotidase;
                     7-methylguanosine phosphate-specific 5'-nucleotidase"
                     /codon_start=1
                     /product="cytosolic 5'-nucleotidase 3A isoform 2"
                     /protein_id="NP_001239303.1"
                     /db_xref="CCDS:CCDS57424.1"
                     /db_xref="GeneID:107569"
                     /db_xref="MGI:MGI:1927186"
                     /translation="MTNQESAVHLKMMPEFQKSSVRIKNPTRVEEIICGLIKGGAAKL
                     QIITDFDMTLSRFSYNGKRCPTCHNIIDNCKLVTDECRRKLLQLKEQYYAIEVDPVLT
                     VEEKFPYMVEWYTKSHGLLIEQGIPKAKLKEIVADSDVMLKEGYENFFGKLQQHGIPV
                     FIFSAGIGDVLEEVIRQAGVYHSNVKVVSNFMDFDENGVLKGFKGELIHVFNKHDGAL
                     KNTDYFSQLKDNSNIILLGDSQGDLRMADGVANVEHILKIGYLNDRVDELLEKYMDSY
                     DIVLVKEESLEVVNSILQKTL"
     exon            105..203
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     exon            204..273
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     exon            274..320
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     exon            321..406
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     exon            407..496
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     exon            497..659
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     exon            660..860
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1519
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1169..1174
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /note="hexamer: ATTAAA"
     polyA_site      1193
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /note="major polyA site"
     regulatory      1405..1410
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /note="hexamer: AATACA"
     polyA_site      1421
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
     regulatory      1502..1507
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /note="hexamer: AAGAAA"
     polyA_site      1519
                     /gene="Nt5c3"
                     /gene_synonym="1600024P05Rik; 2610206B05Rik;
                     3110004A18Rik; cN-III; lupin; Nt5c3a; Nt5y; p36; P5'N-1;
                     P5N-1; PN-1; PN-I; PSN1; UMPH; Umph-1; Umph1"
                     /note="major polyA site"
ORIGIN      
        1 ttttcttttg ctggtgctgc cgtacgtagc ttgtccggtt accttcagaa agattggata
       61 accacgaaat gactaatcag gagtctgctg tccatttgaa aatgatgcca gaatttcaga
      121 aaagctcagt tcgtatcaag aaccctacaa gagtagaaga aattatctgt ggtctcatta
      181 aaggaggcgc tgccaaactt cagataataa cagactttga catgactcta agtaggtttt
      241 cctacaatgg gaaaagatgc ccaacgtgtc ataatatcat tgacaactgt aagcttgtta
      301 cagatgaatg tcgaagaaag ttactgcagc taaaggagca gtattatgct attgaagttg
      361 atcctgttct caccgtggaa gagaagttcc cttacatggt agagtggtat accaaatctc
      421 acggtttgct tattgaacaa ggcataccaa aagccaaact taaagagatt gtggctgact
      481 ctgacgttat gctcaaggaa ggatatgaga atttcttcgg taaacttcaa cagcacggta
      541 tccctgtgtt catattttct gctggtattg gtgatgtact tgaagaagtt atccggcagg
      601 ctggagttta tcactcaaac gtcaaagtag tgtcaaactt catggatttt gatgaaaatg
      661 gagtgctgaa aggattcaaa ggagagttaa tccacgtgtt caacaaacac gatggtgccc
      721 tgaagaatac agactacttc agccagctga aagacaacag caacatcatt ctgctcgggg
      781 actcccaagg agacctaaga atggccgatg gcgtagccaa tgttgaacac attctgaaaa
      841 ttggttatct gaatgacaga gtagacgagc ttttagaaaa atacatggac tcttatgata
      901 ttgttttagt aaaagaagaa tcattggagg ttgtcaactc aattttacag aagacattat
      961 aaacaagcca tcgtgagaag acttccccca ggggtacacc tgggtcaggc actgtccatt
     1021 ttgaggaaag actctttctt atttataaaa cagtcttgct cctggaactt ttcccttttc
     1081 ccaacagacg atcttcagac gtgtggaacc tgtgaaccac ggcccctctt cccacctccg
     1141 tcactatact cagcagaatt tttaagtcat taaaaattat cttaaagcca aaattagaaa
     1201 gatggctccc ttttccaaag tgagttttgt agtttggtgt tctgcagttt ctagggcatt
     1261 ctcccaggtg ggtgtataag ggcgcagaag acgctctctg gatgtattat gttcctgatt
     1321 ttaaccactg ctagctttga aattggggtg gagtatggag acatcatctt ctgaaattgt
     1381 tttatttgtt tattttgtct ataaaataca tttgccagga agttgtgtct taaaaactgt
     1441 tggtgtccgc atcgctaaca ttgcaaatac tctgtgtgaa gcactgagaa taaaccagaa
     1501 aaagaaagct tttctaaaaa aaaaaaaaaa
//
