LOCUS       NM_001085502            1540 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Mus musculus 5'-nucleotidase, cytosolic IA (Nt5c1a), mRNA.
ACCESSION   NM_001085502 XM_112246 XM_907862
VERSION     NM_001085502.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Kviklyte,S., Vertommen,D., Yerna,X., Andersen,H., Xu,X., Gailly,P.,
            Bohlooly-Y,M., Oscarsson,J. and Rider,M.H.
  TITLE     Effects of genetic deletion of soluble 5'-nucleotidases NT5C1A and
            NT5C2 on AMPK activation and nucleotide levels in contracting mouse
            skeletal muscles
  JOURNAL   Am J Physiol Endocrinol Metab 313 (1), E48-E62 (2017)
   PUBMED   28325731
  REMARK    GeneRIF: The results on muscles from the NT5C knockout mice
            contradict previous findings where AMP levels and AMPK activity
            were shown to be modulated by NT5C enzymes.
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Kulkarni,S.S., Karlsson,H.K., Szekeres,F., Chibalin,A.V., Krook,A.
            and Zierath,J.R.
  TITLE     Suppression of 5'-nucleotidase enzymes promotes AMP-activated
            protein kinase (AMPK) phosphorylation and metabolism in human and
            mouse skeletal muscle
  JOURNAL   J Biol Chem 286 (40), 34567-34574 (2011)
   PUBMED   21873433
  REMARK    GeneRIF: NT5C1A suppression promotes AMP-activated protein kinase
            (AMPK) phosphorylation and metabolism in human and mouse skeletal
            muscle
REFERENCE   3  (bases 1 to 1540)
  AUTHORS   Onishi,M., Yasunaga,T., Tanaka,H., Nishimune,Y. and Nozaki,M.
  TITLE     Gene structure and evolution of testicular haploid germ
            cell-specific genes, Oxct2a and Oxct2b
  JOURNAL   Genomics 83 (4), 647-657 (2004)
   PUBMED   15028287
REFERENCE   4  (bases 1 to 1540)
  AUTHORS   Hunsucker,S.A., Spychala,J. and Mitchell,B.S.
  TITLE     Human cytosolic 5'-nucleotidase I: characterization and role in
            nucleoside analog resistance
  JOURNAL   J Biol Chem 276 (13), 10498-10504 (2001)
   PUBMED   11133996
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL606934.12.
            
            On Jun 18, 2019 this sequence version replaced NM_001085502.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC147730.1, SRR7345562.2322666.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-199               AL606934.12        122800-122998
            200-367             AL606934.12        129724-129891
            368-497             AL606934.12        130238-130367
            498-620             AL606934.12        133494-133616
            621-805             AL606934.12        135388-135572
            806-1540            AL606934.12        137157-137891
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 57.4 cM"
     gene            1..1540
                     /gene="Nt5c1a"
                     /gene_synonym="Cn1a; Gm49"
                     /note="5'-nucleotidase, cytosolic IA"
                     /db_xref="GeneID:230718"
                     /db_xref="MGI:MGI:2155700"
     exon            1..199
                     /gene="Nt5c1a"
                     /gene_synonym="Cn1a; Gm49"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..1171
                     /gene="Nt5c1a"
                     /gene_synonym="Cn1a; Gm49"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.89"
                     /EC_number="3.1.3.99"
                     /note="cytosolic 5' nucleotidase, type 1A; cN-IA;
                     cytosolic 5'-nucleotidase IA; 5'-deoxynucleotidase"
                     /codon_start=1
                     /product="cytosolic 5'-nucleotidase 1A"
                     /protein_id="NP_001078971.1"
                     /db_xref="CCDS:CCDS38871.1"
                     /db_xref="GeneID:230718"
                     /db_xref="MGI:MGI:2155700"
                     /translation="MEPGQPREAREPGPGAETAAVPRWEEAKTFYDNLSSKKKPKSPK
                     PQNAVTIAVSSRALFRMDEEQRIYTEQGVEEYVRYQLEHENEPFSPGPAFPFVKALEA
                     VNKRLRELYPDSEDIFDIVLMTNNHAQVGVRLINSINHYDLFIERFCMTGGNSPICYL
                     KAYHTNLYLSADADKVREAIDEGIAAATIFSPSRDVVVSQSQLRVAFDGDAVLFSDES
                     ERIVKAHGLDRFFEHEKAHENKPLAQGPLKGFLEALGRLQKKFYSKGLRLECPIRTYL
                     VTARSAASSGARALKTLRSWGLETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGA
                     QEMGTVAAHVPYGVAQNPRRAAAAKQSLGAQ"
     exon            200..367
                     /gene="Nt5c1a"
                     /gene_synonym="Cn1a; Gm49"
                     /inference="alignment:Splign:2.1.0"
     exon            368..497
                     /gene="Nt5c1a"
                     /gene_synonym="Cn1a; Gm49"
                     /inference="alignment:Splign:2.1.0"
     exon            498..620
                     /gene="Nt5c1a"
                     /gene_synonym="Cn1a; Gm49"
                     /inference="alignment:Splign:2.1.0"
     exon            621..805
                     /gene="Nt5c1a"
                     /gene_synonym="Cn1a; Gm49"
                     /inference="alignment:Splign:2.1.0"
     exon            806..1540
                     /gene="Nt5c1a"
                     /gene_synonym="Cn1a; Gm49"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcccggtgc cgagagctcc gcgtctccac tgctgtcttc tcccgccggg ccggatagaa
       61 tccgagtcgg agcatggaac cagggcagcc ccgggaggcc cgcgaacccg ggccaggggc
      121 agagaccgcc gcggtaccgc gctgggagga agccaagact ttctatgaca acctctcgtc
      181 caagaagaaa cccaaatcgc caaagcctca gaatgcggtc accatcgcgg tgtcctccag
      241 agccttgttc cgaatggatg aggaacagag gatctacacg gagcagggtg tcgaggagta
      301 cgtgcgatac cagctggagc acgagaatga gcccttcagc cccgggcctg ccttcccctt
      361 tgtgaaggcc ctggaggctg tgaacaagcg acttcgggag ttgtaccctg atagcgaaga
      421 tatctttgac attgtcctca tgaccaacaa ccatgctcag gtgggagttc gtctcatcaa
      481 cagtatcaac cattatgacc tgttcattga gcgattctgc atgacaggtg ggaatagccc
      541 catctgctac ctcaaggcct accacaccaa cctctacttg tcagccgatg cagacaaagt
      601 gcgagaagcc atcgatgagg ggattgcagc tgccaccatc ttcagcccca gcagggatgt
      661 ggttgtgtcc cagagtcagc ttcgagtggc cttcgatggg gacgcagtac tcttctccga
      721 tgagtcagag cgcatcgtca aggcccatgg gctggacaga ttctttgagc atgagaaggc
      781 ccacgagaac aaacctttgg cgcagggccc cctaaagggc ttcctggaag cactgggtag
      841 actgcaaaag aaattctact ccaagggcct gcgtctggag tgcccgattc gcacgtattt
      901 ggtgaccgcg cgaagtgcag ccagttccgg agcccgggcc ctcaagaccc tgcgcagctg
      961 gggcctggag acggatgagg ccttattcct agctggagca cccaagggtc cccttcttga
     1021 gaagatccgt ccacacatct tctttgatga ccagatgttc cacgtggctg gagctcagga
     1081 gatgggcacg gtggccgccc atgtgcctta tggtgtggct cagaatcccc ggcgggctgc
     1141 cgctgcgaag cagtcattag gtgcacagta gctgagccac cttgctccag gccccctgtc
     1201 acactttctg aacttcatct ggtgaacttt tctggctcct caccgtccac cctcctgcat
     1261 gctgccgtca accctccatg ccaggtactc ttagaaagtt atgaaaacag aaccagcagt
     1321 ctcaaaaccc cttcggggca aacacagcca tgaccctggc tcaaaagcag ggctctgcag
     1381 ggtagtccgg ctgtcaggga gaaattggtg ggagagagag tggtgtggtc agagaagcca
     1441 ggatgtcaaa gggcttggaa tggaaaatgt ctcatgtagt gcaaagaaca cattgtgtga
     1501 gagtgggtag atttaacatc taaagcgaat ctagcatgaa
//
