LOCUS       NM_015807                824 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus 5',3'-nucleotidase, cytosolic (Nt5c), transcript
            variant 1, mRNA.
ACCESSION   NM_015807
VERSION     NM_015807.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 824)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 824)
  AUTHORS   Rampazzo,C., Kost-Alimova,M., Ruzzenente,B., Dumanski,J.P. and
            Bianchi,V.
  TITLE     Mouse cytosolic and mitochondrial deoxyribonucleotidases: cDNA
            cloning of the mitochondrial enzyme, gene structures, chromosomal
            mapping and comparison with the human orthologs
  JOURNAL   Gene 294 (1-2), 109-117 (2002)
   PUBMED   12234672
REFERENCE   4  (bases 1 to 824)
  AUTHORS   Rampazzo,C., Johansson,M., Gallinaro,L., Ferraro,P., Hellman,U.,
            Karlsson,A., Reichard,P. and Bianchi,V.
  TITLE     Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and
            overexpression of the enzyme in Escherichia coli and mammalian
            cells
  JOURNAL   J Biol Chem 275 (8), 5409-5415 (2000)
   PUBMED   10681516
REFERENCE   5  (bases 1 to 824)
  AUTHORS   Astakhova,N.M., Zhdanova,N.S., Kaftanovsiaia,E.M., Kuznetsov,S.B.
            and Serov,O.L.
  TITLE     [Preliminary localization of TK1 and UMPH2 genes on swine
            chromosome 12]
  JOURNAL   Genetika 30 (6), 832-838 (1994)
   PUBMED   7958795
REFERENCE   6  (bases 1 to 824)
  AUTHORS   Yasue,M., Serikawa,T. and Yamada,J.
  TITLE     Chromosomal assignments of 23 biochemical loci of the rat by using
            rat x mouse somatic cell hybrids
  JOURNAL   Cytogenet Cell Genet 57 (2-3), 142-148 (1991)
   PUBMED   1914521
REFERENCE   7  (bases 1 to 824)
  AUTHORS   Pack,S.D., Zhdanova,N.S., Sukoyan,M.A. and Serov,O.L.
  TITLE     Chromosomal and regional localization of the genes for UMPH2, APRT,
            PEPD, PEPS, PSP, and PGP in mink: comparison with man and mouse
  JOURNAL   Cytogenet Cell Genet 50 (2-3), 127-131 (1989)
   PUBMED   2776480
REFERENCE   8  (bases 1 to 824)
  AUTHORS   Wilson,D.E., Woodard,D., Sandler,A., Erickson,J. and Gurney,A.
  TITLE     Provisional assignment of the gene for uridine monophosphatase-2
            (Umph-2) to mouse chromosome 11
  JOURNAL   Biochem Genet 25 (1-2), 1-6 (1987)
   PUBMED   3034234
REFERENCE   9  (bases 1 to 824)
  AUTHORS   Munke,M. and Francke,U.
  TITLE     The physical map of Mus musculus chromosome 11 reveals evolutionary
            relationships with different syntenic groups of genes in Homo
            sapiens
  JOURNAL   J Mol Evol 25 (2), 134-140 (1987)
   PUBMED   3116273
REFERENCE   10 (bases 1 to 824)
  AUTHORS   Swallow,D.M., Turner,V.S. and Hopkinson,D.A.
  TITLE     Isozymes of rodent 5'-nucleotidase: evidence for two independent
            structural loci Umph-1 and Umph-2
  JOURNAL   Ann Hum Genet 47 (1), 9-17 (1983)
   PUBMED   6301357
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645470.20.
            
            On Mar 7, 2023 this sequence version replaced NM_015807.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF078840.1, BC024368.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AL645470.20        132991-133217
            228-328             AL645470.20        133453-133553
            329-389             AL645470.20        133626-133686
            390-504             AL645470.20        133857-133971
            505-824             AL645470.20        134080-134399
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 80.84 cM"
     gene            1..824
                     /gene="Nt5c"
                     /gene_synonym="Dnt; Dnt1; Umph-2; Umph2"
                     /note="5',3'-nucleotidase, cytosolic"
                     /db_xref="GeneID:50773"
                     /db_xref="MGI:MGI:1354954"
     exon            1..227
                     /gene="Nt5c"
                     /gene_synonym="Dnt; Dnt1; Umph-2; Umph2"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..650
                     /gene="Nt5c"
                     /gene_synonym="Dnt; Dnt1; Umph-2; Umph2"
                     /EC_number="3.1.3.5"
                     /note="isoform 1 is encoded by transcript variant 1; 5'
                     nucleotidase, deoxy (pyrimidine), cytosolic type C;
                     5'(3')-deoxyribonucleotidase, cytosolic type; dNT-1;
                     deoxy-5'-nucleotidase 1; cytosolic 5',3'-pyrimidine
                     nucleotidase; uridine monophosphatase 2"
                     /codon_start=1
                     /product="5'(3')-deoxyribonucleotidase, cytosolic type
                     isoform 1"
                     /protein_id="NP_056622.1"
                     /db_xref="CCDS:CCDS25637.1"
                     /db_xref="GeneID:50773"
                     /db_xref="MGI:MGI:1354954"
                     /translation="MAVKRPVRVLVDMDGVLADFESGLLQGFRRRFPEEPHVPLEQRR
                     GFLANEQYGALRPDLAEKVASVYESPGFFLNLEPIPGALDALREMNDMKDTEVFICTT
                     PLLKYDHCVGEKYRWVEQNLGPEFVERIILTRDKTVVMGDLLIDDKDNIQGLEETPSW
                     EHILFTCCHNQHLALPPTRRRLLSWSDNWRGIIESKRASL"
     exon            228..328
                     /gene="Nt5c"
                     /gene_synonym="Dnt; Dnt1; Umph-2; Umph2"
                     /inference="alignment:Splign:2.1.0"
     exon            329..389
                     /gene="Nt5c"
                     /gene_synonym="Dnt; Dnt1; Umph-2; Umph2"
                     /inference="alignment:Splign:2.1.0"
     exon            390..504
                     /gene="Nt5c"
                     /gene_synonym="Dnt; Dnt1; Umph-2; Umph2"
                     /inference="alignment:Splign:2.1.0"
     exon            505..824
                     /gene="Nt5c"
                     /gene_synonym="Dnt; Dnt1; Umph-2; Umph2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      806..811
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nt5c"
                     /gene_synonym="Dnt; Dnt1; Umph-2; Umph2"
                     /note="hexamer: AATAAA"
     polyA_site      824
                     /gene="Nt5c"
                     /gene_synonym="Dnt; Dnt1; Umph-2; Umph2"
                     /note="major polyA site"
ORIGIN      
        1 atcttccgtg cttgccgctg tgctctgcgg agacaccggg cgtggcaatg gcggtgaagc
       61 ggcccgtgcg cgtgctggtg gacatggacg gcgtgctagc tgatttcgag tccggcctcc
      121 tgcagggttt tcgccgccgc tttcccgagg aaccgcacgt gccgctggaa cagcgccggg
      181 gcttcctcgc caacgagcag tacggagctc tgaggccgga cctggcggaa aaagtggcca
      241 gtgtgtatga atctccaggc tttttcctaa acttggagcc catccctggg gccttggacg
      301 ctttgcgaga gatgaacgac atgaaagaca ccgaggtctt catctgcacc accccactgc
      361 tgaagtacga ccactgtgtg ggcgagaagt accgctgggt ggagcagaac ctggggccag
      421 agtttgtgga gcggattatc ttgactaggg acaagacggt ggtcatggga gacctgctca
      481 ttgatgacaa agacaacatt caaggcctag aggagacccc aagctgggag cacatcttgt
      541 tcacctgctg ccacaatcag cacctggccc tgccccccac caggagacgg ctgctttcct
      601 ggagtgacaa ctggaggggg atcattgaga gcaagcgagc cagcctgtga aggaccctga
      661 ggtggacagg accctgcctg gacaggtgac tttctttaat cagggagcca cttgaacaga
      721 ctgctttccg gctcctccca ggaggcctac acgggaaaga aggagactag cacacaaata
      781 cagtggagaa aatcaggagg tttttaataa aaataaaaac caaa
//
