LOCUS       NM_028142               2209 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4),
            transcript variant 1, mRNA.
ACCESSION   NM_028142
VERSION     NM_028142.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Metodiev,M.D., Spahr,H., Loguercio Polosa,P., Meharg,C., Becker,C.,
            Altmueller,J., Habermann,B., Larsson,N.G. and Ruzzenente,B.
  TITLE     NSUN4 is a dual function mitochondrial protein required for both
            methylation of 12S rRNA and coordination of mitoribosomal assembly
  JOURNAL   PLoS Genet 10 (2), e1004110 (2014)
   PUBMED   24516400
  REMARK    GeneRIF: The presented data suggest that NSUN4 has a key role in
            controlling a final step in ribosome biogenesis to ensure that only
            the mature SSU and LSU are assembled.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Chi,L. and Delgado-Olguin,P.
  TITLE     Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase
            family genes in early mouse embryogenesis
  JOURNAL   Gene Expr Patterns 13 (8), 319-327 (2013)
   PUBMED   23816522
REFERENCE   3  (bases 1 to 2209)
  AUTHORS   Camara,Y., Asin-Cayuela,J., Park,C.B., Metodiev,M.D., Shi,Y.,
            Ruzzenente,B., Kukat,C., Habermann,B., Wibom,R., Hultenby,K.,
            Franz,T., Erdjument-Bromage,H., Tempst,P., Hallberg,B.M.,
            Gustafsson,C.M. and Larsson,N.G.
  TITLE     MTERF4 regulates translation by targeting the methyltransferase
            NSUN4 to the mammalian mitochondrial ribosome
  JOURNAL   Cell Metab 13 (5), 527-539 (2011)
   PUBMED   21531335
REFERENCE   4  (bases 1 to 2209)
  AUTHORS   Jamsai,D., Rijal,S., Bianco,D.M., O'Connor,A.E., Merriner,D.J.,
            Smith,S.J., Gibbs,G.M. and O'Bryan,M.K.
  TITLE     A novel protein, sperm head and tail associated protein (SHTAP),
            interacts with cysteine-rich secretory protein 2 (CRISP2) during
            spermatogenesis in the mouse
  JOURNAL   Biol Cell 102 (2), 93-106 (2009)
   PUBMED   19686095
  REMARK    GeneRIF: expression and localization of SHTAP in testis and sperm
            raise the possibility that the SHTAP-CRISP2 complex has a potential
            role in sperm formation and/or function.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL627105.13.
            
            On Mar 18, 2019 this sequence version replaced NM_028142.4.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024628.1, AK012994.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               AL627105.13        142622-142749       c
            129-463             AL627105.13        137268-137602       c
            464-618             AL627105.13        133623-133777       c
            619-779             AL627105.13        129007-129167       c
            780-904             AL627105.13        124690-124814       c
            905-2209            AL627105.13        121945-123249       c
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 53.1 cM"
     gene            1..2209
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /note="NOL1/NOP2/Sun domain family, member 4"
                     /db_xref="GeneID:72181"
                     /db_xref="MGI:MGI:1919431"
     exon            1..128
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..1181
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /note="isoform a is encoded by transcript variant 1; sperm
                     head and tail associated protein; putative
                     methyltransferase NSUN4; 5-methylcytosine tRNA
                     methyltransferase NSUN4; 5-cytosine tRNA methyltransferase
                     NSUN4"
                     /codon_start=1
                     /product="putative methyltransferase NSUN4 isoform a"
                     /protein_id="NP_082418.1"
                     /db_xref="CCDS:CCDS18502.1"
                     /db_xref="GeneID:72181"
                     /db_xref="MGI:MGI:1919431"
                     /translation="MAAPVLRCVRKLLKLVDFTPVPRRYRYKKKWATTEPQFTASRLA
                     LQNFDMTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDSVSAKLEQLSAKDFVSEA
                     ISHQKLEPESGLSPTPSLDCSPNLRCFTFSRGDVSRFPPARLGSLGLMDYYLMDAASL
                     LPVLALGLQHGDTVLDLCAAPGGKTLALLQTGCCRNLAANDLSTSRTGRLQKVLHSYV
                     PQDIREGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFQRSRKK
                     ERQMLPMLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYNIKVQ
                     VEDLSHFRKLFMDTFCFFPSCQVGELVIPNLMVNFGPMYFCKLHRLP"
     exon            129..463
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /inference="alignment:Splign:2.1.0"
     exon            464..618
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /inference="alignment:Splign:2.1.0"
     exon            619..779
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /inference="alignment:Splign:2.1.0"
     exon            780..904
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /inference="alignment:Splign:2.1.0"
     exon            905..2209
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1459..1464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /note="hexamer: AATAAA"
     polyA_site      1482
                     /gene="Nsun4"
                     /gene_synonym="2310010O12Rik; 2810405F18Rik; Shtap"
                     /note="major polyA site"
ORIGIN      
        1 agtttcagtc ggaattattg gcgttgttag ttgacatggc tgcgcccgtc ttaagatgtg
       61 ttcggaagct gctgaagctc gtggacttca cgccggtccc gcggagatat cgatataaga
      121 agaaatgggc taccacagaa ccacaattca cggccagtcg gcttgctctg cagaattttg
      181 acatgaccta cagtgtacag tttggggatc tttggccatc aatccgtgtt agtcttctct
      241 cagagcagaa gtatggtgca ttggtcaata attttgctgc ttgggatagt gtgagtgcta
      301 agctggagca gctgagtgcc aaggacttcg tcagtgaagc catctctcac cagaaactgg
      361 agcctgagag tggcctctct ccaactccat ccctggactg cagcccaaat cttcgatgct
      421 tcactttttc cagaggggac gtcagccgct ttcctcctgc cagacttggc agcctgggtc
      481 tcatggacta ctacctgatg gatgctgcct ccttgctccc tgtcctggct cttggtctgc
      541 agcatggaga cactgtgctt gatctgtgtg ctgctcctgg gggcaaaacg ctggcattac
      601 ttcagacggg ttgttgccgt aatctagctg ccaacgacct ctccacttcc cgaacaggca
      661 gactgcagaa ggtccttcac agttatgtgc ctcaagatat tagggaaggg aaccaagtcc
      721 gagttacctc atgggatggc aggaagtggg gagaactgga gggggacacc tatgataggg
      781 tgttagtaga tgtgccatgt accacggacc gccattccct tcatgaggaa gagaacaaca
      841 tctttcagcg gtcaaggaag aaggagcgac agatgttgcc tatgctgcag gtgcagcttc
      901 ttgcggctgg actccttgcc accaaaccag gaggtcacgt tgtctattca acttgctcac
      961 tgtctcactt acagaatgag tatgtggtgc aaggtgccat tgagcttctg gccaatcaat
     1021 acaacatcaa ggttcaagtg gaagacctgt ctcacttccg aaagcttttc atggacacat
     1081 tctgtttctt cccatcctgc caggttgggg agctggtaat accaaacctc atggtcaatt
     1141 ttggacctat gtacttctgc aaactgcata ggctgccata gtgccaccct gtccctgaag
     1201 taggaaggtg atgactccat tgaaactgag gctagtggca ggtgtatccc aaggcctgtc
     1261 tccatccatt acatctaaag atagttgcaa acagcaggca gtagggactg ctgcccagct
     1321 ctggattatc agcaggtttc taactcatgt ctcatgtctc ttagcctcta ctaaggccct
     1381 tgctccatta gggattaaga cagaatctgt gaatgagccc atagtttaag ctgtaaattt
     1441 gggaagaaac atttagccaa taaagttgtt gaagtgctct aagggtgggc ctgcagttgg
     1501 gtacatcaca tcagtctaag ttctattatg tttatgtgtc agctgaagcc gccaagcaca
     1561 ctctccaaag tgcccagcca ggcctccagt cctcccacac tggtgagttc catttaatca
     1621 acccacaggc ccgccagcct ccccaagtgt ctccatatgg cacatggtct aatttaagga
     1681 ataagtaact gaagcggttg agcctgtggc tgatgatcct cttacagaga gagctttcct
     1741 gagtgctctg gctcgtcttc agatgcggct gcaaaagctg cttgtggtac aggttggttt
     1801 tgtatgtttc ttcttttatt tcttcatata tcagcaagtg ttctgggaat tcctatatga
     1861 caactatgga gctgttagag agatggttgg gaccatggta aatagttgga tttcctcatc
     1921 ttcagaatgg caacctactt cttttcgctc tccaagtacc tcggagtttc ctgacatttt
     1981 atttcaagag tcttgatttt atttctagtc tgtggcccag ctctggcgcc tgctttggct
     2041 catctgcaca tcctctagag ttctgagttc ttccacatag caggctttcc tcacacgcgg
     2101 tgttttgggg tgacattacg taaggtgact tgcgtgctga ttgttttgta acacttgtca
     2161 gaacaaagca acgtttagag ccaggaaaca gtaaacattg cttatggtc
//
