LOCUS       NM_178925               3815 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus NOL1/NOP2/Sun domain family member 3 (Nsun3),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_178925
VERSION     NM_178925.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Murakami,Y., Wei,F.Y., Kawamura,Y., Horiguchi,H., Kadomatsu,T.,
            Miyata,K., Miura,K., Oike,Y., Ando,Y., Ueda,M., Tomizawa,K. and
            Chujo,T.
  TITLE     NSUN3-mediated mitochondrial tRNA 5-formylcytidine modification is
            essential for embryonic development and respiratory complexes in
            mice
  JOURNAL   Commun Biol 6 (1), 307 (2023)
   PUBMED   36949224
  REMARK    GeneRIF: NSUN3-mediated mitochondrial tRNA 5-formylcytidine
            modification is essential for embryonic development and respiratory
            complexes in mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Trixl,L., Amort,T., Wille,A., Zinni,M., Ebner,S., Hechenberger,C.,
            Eichin,F., Gabriel,H., Schoberleitner,I., Huang,A., Piatti,P.,
            Nat,R., Troppmair,J. and Lusser,A.
  TITLE     RNA cytosine methyltransferase Nsun3 regulates embryonic stem cell
            differentiation by promoting mitochondrial activity
  JOURNAL   Cell Mol Life Sci 75 (8), 1483-1497 (2018)
   PUBMED   29103146
  REMARK    GeneRIF: Nsun3 regulates embryonic stem cell differentiation by
            promoting mitochondrial activity.
REFERENCE   3  (bases 1 to 3815)
  AUTHORS   Chi,L. and Delgado-Olguin,P.
  TITLE     Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase
            family genes in early mouse embryogenesis
  JOURNAL   Gene Expr Patterns 13 (8), 319-327 (2013)
   PUBMED   23816522
REFERENCE   4  (bases 1 to 3815)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154309.1.
            
            On Mar 28, 2023 this sequence version replaced NM_178925.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK032127.1, SRR13422601.806134.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-109               AC154309.1         117003-117111       c
            110-219             AC154309.1         116120-116229       c
            220-563             AC154309.1         106620-106963       c
            564-718             AC154309.1         100990-101144       c
            719-840             AC154309.1         99939-100060        c
            841-3815            AC154309.1         62776-65750         c
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 36.43 cM"
     gene            1..3815
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /note="NOL1/NOP2/Sun domain family member 3"
                     /db_xref="GeneID:106338"
                     /db_xref="MGI:MGI:2146565"
     exon            1..109
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..1144
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NOL1/NOP2/Sun domain family 3; putative methyltransferase
                     NSUN3; tRNA (cytosine(34)-C(5))-methyltransferase,
                     mitochondrial"
                     /codon_start=1
                     /product="tRNA (cytosine(34)-C(5))-methyltransferase,
                     mitochondrial isoform 1"
                     /protein_id="NP_849256.1"
                     /db_xref="CCDS:CCDS37373.1"
                     /db_xref="GeneID:106338"
                     /db_xref="MGI:MGI:2146565"
                     /translation="MLTRLKAKSEGKLAKQLCRVVLDQFDKQYSKELGDSWSTVRDVL
                     ISPSLWQYAILFNRFNYPFELEKALHLRGYHTVLQGALPHYPKSMKCYLSRTPDRMPS
                     ERHQTGSLKKYYLLNAASLLPVLALELRDGEAVLDLCAAPGGKSVALLQCAYPGYLLC
                     NEYDRPRGRWLRQTLESFIPQPLINVIKVSELDGREMGDAQPATFDKVLVDAPCSNDR
                     SWLFSSDSQKAAYRIHQRKNLPVLQVELVRSAIKALRPGGLLVYSTCTLSKAENQDVI
                     SEVLTSDSNIVPVDISGIARTFSQDFTFAPTDQKCSLLVIPEKGKAWGPMYIAKLKKG
                     MSTRKRQGEFCKPC"
     exon            110..219
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            220..563
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            564..718
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            719..840
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            841..3815
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3793..3798
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3815
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtgacctcct cgcaggaccc ggaagtatgg cgtttcttcg tgttaccccg cgcgaactct
       61 agcggagacc cgtagttggc gagctcaggc ggggaccatg ctgactcggc tgaaagcaaa
      121 atcagagggg aagcttgcca aacagctatg cagagttgtg ttggatcagt ttgacaaaca
      181 gtattcgaaa gaacttgggg attcctggag tacagtaagg gatgttctga tatctccatc
      241 actatggcaa tatgccatcc tcttcaaccg attcaattat ccctttgaac tggaaaaggc
      301 cttgcatctg aggggctatc acacagtcct tcaaggggct ttaccacatt atcctaaatc
      361 aatgaagtgt tacctcagcc gaaccccaga ccgaatgcct tcagaaagac atcagactgg
      421 aagcttgaaa aagtactacc tccttaatgc tgcttctctt ctcccagtac tggctctgga
      481 actgagagac ggggaggcag tcctggactt gtgtgctgct cctggaggca aatccgtagc
      541 tctgctgcaa tgtgcttatc caggttatct tctctgtaac gagtatgatc gcccgagagg
      601 aagatggctg agacagacgt tggaatcttt catcccacag cctttgataa atgtaattaa
      661 agtgtctgaa ttggatggca gagaaatggg agatgcccag cctgcaacat ttgacaaggt
      721 gttagttgac gctccatgtt caaatgatcg aagctggctg ttctcgtcgg actctcagaa
      781 agctgcctat aggatccatc aaaggaagaa tttgcctgtc ctacaggtag aactcgtaag
      841 gtctgcaatt aaagccttgc gccccggagg gctcctcgtg tactctacat gcacgctttc
      901 caaggctgaa aatcaagatg tgatcagcga ggtcttaact tccgacagta acatcgtacc
      961 tgtggatatt agtgggatag caaggacttt ctcccaagac ttcacatttg cccctactga
     1021 ccagaaatgc agccttttgg tgattccgga aaagggcaaa gcttggggcc caatgtatat
     1081 agccaaactg aaaaaaggga tgagtacaag aaaaaggcaa ggcgaatttt gtaagccatg
     1141 ctgatgtgtt attgaatgca tattttctga gctcaccacg tgttagttgt taggtgtagc
     1201 tctttgcttc tctgccttag ttccaggaat ggactgagct accagctccc acccaccttc
     1261 ccttgaatcc acggataaaa gtcgagcgcg cgcgcgcgca cacacacaca cacacacaca
     1321 cacacacaca catcacacgg ctattccttt tcaacttacc ttcttggcac aattgctgca
     1381 tgctgttatc tcccgcctgg aaaaacatgc tcttatcagt attcttagtc cacatctccc
     1441 acccacccta aactttagct gctaaactcc cctgaccacc ggtctataag agcagcctgt
     1501 gtggccaccc cattttgaga tctcacatgg ctggacaact tttctccatc agaagaccag
     1561 caaactctgt ctctccttcc ttgtgtctgt cttttttcct tcagggacct ggaagtttca
     1621 cttattcctt cctccccaca attggctatg gcttctttac tgacagatca agaaccaatt
     1681 gaggaacagg acagcatcag aaccgcccct acagttagtg tttaactact tacagagcca
     1741 ctttccctag gaccacttgg cttctgttta gttagtattt cagcctcaat gagcatagga
     1801 ttaagaaatt ttcaggtaca attttgctcc tggaaatata tgtatagtga tgatttaaag
     1861 tgggacagat ggtgttggtt ctgtataata attttttgtc ttttaattgg gactttatag
     1921 ataaattaat tagaatactg agttctgtgc ataaaatgtt taggaccgta ttctataaca
     1981 tgagtgtttt aaatctttcc attaaaaatg tgtatttgtc aacactttta ctgtttcctc
     2041 atcaaatctg cagaatcttt tagtaggaat aattggtatt cggaggctaa cttcttaagc
     2101 agcacaattt ctagttctag caaaataggt ttcttgtttg cggagttgtt ctgtgttaag
     2161 ttaaggactg tcttctgttt actgcagagg gcagtgactc tgttgacacc agcttgattt
     2221 ctccccattt gcatttattc acaaatacaa actgaaaggg taaattatgt tcaaaggcct
     2281 ttagagcaac agattcacac gcacgcattt gactttcgac attcagcttg tagaataatg
     2341 gagtctatcc agcaggctat aaagcaagca cttgaaacaa aaccagaatg attaataacg
     2401 gcaataactg aactatattt ggagtgtttt gttacataat tgcagtcata atgagtacta
     2461 cagaatctga ttatcttaca gttgcaatct ggagtggctt tttatttcac ttggttataa
     2521 aatgttatat gagagtgtga tgtcacatat taatatagaa gatgcctacc taataagagt
     2581 caattcttta ttaccaagac cccaaaccat tcagagtggt ctctatttac acatatccca
     2641 agaattttgt gtgtgcttct atatttatac ttaggctttt ttaaaatcaa ttttcaatct
     2701 taaatttttc tttagaaatg cttcagttgg atccaaacat ttttccaact attaagaaaa
     2761 atcacagtgg cagacatggc actgcacacc tgtagtcaca gcattttggg ggctgaggtg
     2821 gaagaattat aaattcaagt cagccttggc tgcctagcaa gaccctcctc aaggatgatg
     2881 tagggaggag agagggaagg gggaaggcta gagcaggaga gaaaaatcac aaagcatact
     2941 tgaaattgga aattagcttt ttattttcat taagatttat ttatttttat cttattttat
     3001 atgtataaat atcttggcaa catgtatgtc tgcgtaccat gtgcatacct gttgcatggg
     3061 gaagtcagaa ctggacatca ggtcctccag aactgagata tatagatggt tttgaaccac
     3121 catataggtg cttggaatca aaagctcgtc ttctgtataa gcaacaagtg ctgttaactg
     3181 ctgagctatc tctcaagccc aaaagtagct taaaaaaatc acctgtggaa gcacaaaatt
     3241 ccatggatcc agagtggaga gtgttctgtt ctatgcatta agctatccag tacatacttt
     3301 ttgttttgca catctgtgta actggagtca aatgaatgac agtctaggaa aaatcatcta
     3361 cctttgttct tgacaatatc ttaaactata tctgaccagt gtgtttatag taaatgttta
     3421 tctcttgggc ctatctaatg tattcagtta atacagaaga ttaatgatag aaaaggtttt
     3481 tttttttgtt ttgttttgtt ttagttggaa gtagtgtttg tatttctgct tgaattcctg
     3541 tctttggatt ctgtagtttt aaaagtgctt ttgtataagt taaccacaat gtaaatcttc
     3601 acagtacaca cacagttgtg tgtgttgaat gtcattattt ttccagtata atgtcatgat
     3661 acttcaaatg aacactaggt gtggtattgg acttgattat cttgggattg atgaaaatat
     3721 atgtcttaat gcatatattc atcgacttta ttattaagat gattgtaaca aaacagcaaa
     3781 catttattga gcattaaatg cttgattgaa gtctt
//
