LOCUS       NM_001419112            4035 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus NOL1/NOP2/Sun domain family member 3 (Nsun3),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001419112
VERSION     NM_001419112.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Murakami,Y., Wei,F.Y., Kawamura,Y., Horiguchi,H., Kadomatsu,T.,
            Miyata,K., Miura,K., Oike,Y., Ando,Y., Ueda,M., Tomizawa,K. and
            Chujo,T.
  TITLE     NSUN3-mediated mitochondrial tRNA 5-formylcytidine modification is
            essential for embryonic development and respiratory complexes in
            mice
  JOURNAL   Commun Biol 6 (1), 307 (2023)
   PUBMED   36949224
  REMARK    GeneRIF: NSUN3-mediated mitochondrial tRNA 5-formylcytidine
            modification is essential for embryonic development and respiratory
            complexes in mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Trixl,L., Amort,T., Wille,A., Zinni,M., Ebner,S., Hechenberger,C.,
            Eichin,F., Gabriel,H., Schoberleitner,I., Huang,A., Piatti,P.,
            Nat,R., Troppmair,J. and Lusser,A.
  TITLE     RNA cytosine methyltransferase Nsun3 regulates embryonic stem cell
            differentiation by promoting mitochondrial activity
  JOURNAL   Cell Mol Life Sci 75 (8), 1483-1497 (2018)
   PUBMED   29103146
  REMARK    GeneRIF: Nsun3 regulates embryonic stem cell differentiation by
            promoting mitochondrial activity.
REFERENCE   3  (bases 1 to 4035)
  AUTHORS   Chi,L. and Delgado-Olguin,P.
  TITLE     Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase
            family genes in early mouse embryogenesis
  JOURNAL   Gene Expr Patterns 13 (8), 319-327 (2013)
   PUBMED   23816522
REFERENCE   4  (bases 1 to 4035)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154309.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.2496317.1,
                                           SRR13422603.378114.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-329               AC154309.1         116783-117111       c
            330-439             AC154309.1         116120-116229       c
            440-783             AC154309.1         106620-106963       c
            784-938             AC154309.1         100990-101144       c
            939-1060            AC154309.1         99939-100060        c
            1061-4035           AC154309.1         62776-65750         c
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 36.43 cM"
     gene            1..4035
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /note="NOL1/NOP2/Sun domain family member 3"
                     /db_xref="GeneID:106338"
                     /db_xref="MGI:MGI:2146565"
     exon            1..329
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             318..1364
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /note="isoform 2 is encoded by transcript variant 2;
                     NOL1/NOP2/Sun domain family 3; putative methyltransferase
                     NSUN3; tRNA (cytosine(34)-C(5))-methyltransferase,
                     mitochondrial"
                     /codon_start=1
                     /product="tRNA (cytosine(34)-C(5))-methyltransferase,
                     mitochondrial isoform 2"
                     /protein_id="NP_001406041.1"
                     /db_xref="GeneID:106338"
                     /db_xref="MGI:MGI:2146565"
                     /translation="MLISLKAKSEGKLAKQLCRVVLDQFDKQYSKELGDSWSTVRDVL
                     ISPSLWQYAILFNRFNYPFELEKALHLRGYHTVLQGALPHYPKSMKCYLSRTPDRMPS
                     ERHQTGSLKKYYLLNAASLLPVLALELRDGEAVLDLCAAPGGKSVALLQCAYPGYLLC
                     NEYDRPRGRWLRQTLESFIPQPLINVIKVSELDGREMGDAQPATFDKVLVDAPCSNDR
                     SWLFSSDSQKAAYRIHQRKNLPVLQVELVRSAIKALRPGGLLVYSTCTLSKAENQDVI
                     SEVLTSDSNIVPVDISGIARTFSQDFTFAPTDQKCSLLVIPEKGKAWGPMYIAKLKKG
                     MSTRKRQGEFCKPC"
     exon            330..439
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            440..783
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            784..938
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1060
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..4035
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4013..4018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4035
                     /gene="Nsun3"
                     /gene_synonym="6720484A09Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtgacctcct cgcaggaccc ggaagtatgg cgtttcttcg tgttaccccg cgcgaactct
       61 agcggagacc cgtagttggc gagctcaggc ggggaccatg ctgactcggg tgaggctctg
      121 gcggtcgagc gccccgggct gaagccaccg agaggcaggg tggactggga tgggacccgc
      181 actgcccaca gcccggaacc gtgctgagtg tgttcctggt gctgctgctg tcccgcgggc
      241 cggcgacatt gtcactgcga gctttctcag ccctgggtgc accccggtga tgaggggaga
      301 gccagaaagt tggggcgatg cttatttccc tgaaagcaaa atcagagggg aagcttgcca
      361 aacagctatg cagagttgtg ttggatcagt ttgacaaaca gtattcgaaa gaacttgggg
      421 attcctggag tacagtaagg gatgttctga tatctccatc actatggcaa tatgccatcc
      481 tcttcaaccg attcaattat ccctttgaac tggaaaaggc cttgcatctg aggggctatc
      541 acacagtcct tcaaggggct ttaccacatt atcctaaatc aatgaagtgt tacctcagcc
      601 gaaccccaga ccgaatgcct tcagaaagac atcagactgg aagcttgaaa aagtactacc
      661 tccttaatgc tgcttctctt ctcccagtac tggctctgga actgagagac ggggaggcag
      721 tcctggactt gtgtgctgct cctggaggca aatccgtagc tctgctgcaa tgtgcttatc
      781 caggttatct tctctgtaac gagtatgatc gcccgagagg aagatggctg agacagacgt
      841 tggaatcttt catcccacag cctttgataa atgtaattaa agtgtctgaa ttggatggca
      901 gagaaatggg agatgcccag cctgcaacat ttgacaaggt gttagttgac gctccatgtt
      961 caaatgatcg aagctggctg ttctcgtcgg actctcagaa agctgcctat aggatccatc
     1021 aaaggaagaa tttgcctgtc ctacaggtag aactcgtaag gtctgcaatt aaagccttgc
     1081 gccccggagg gctcctcgtg tactctacat gcacgctttc caaggctgaa aatcaagatg
     1141 tgatcagcga ggtcttaact tccgacagta acatcgtacc tgtggatatt agtgggatag
     1201 caaggacttt ctcccaagac ttcacatttg cccctactga ccagaaatgc agccttttgg
     1261 tgattccgga aaagggcaaa gcttggggcc caatgtatat agccaaactg aaaaaaggga
     1321 tgagtacaag aaaaaggcaa ggcgaatttt gtaagccatg ctgatgtgtt attgaatgca
     1381 tattttctga gctcaccacg tgttagttgt taggtgtagc tctttgcttc tctgccttag
     1441 ttccaggaat ggactgagct accagctccc acccaccttc ccttgaatcc acggataaaa
     1501 gtcgagcgcg cgcgcgcgca cacacacaca cacacacaca cacacacaca catcacacgg
     1561 ctattccttt tcaacttacc ttcttggcac aattgctgca tgctgttatc tcccgcctgg
     1621 aaaaacatgc tcttatcagt attcttagtc cacatctccc acccacccta aactttagct
     1681 gctaaactcc cctgaccacc ggtctataag agcagcctgt gtggccaccc cattttgaga
     1741 tctcacatgg ctggacaact tttctccatc agaagaccag caaactctgt ctctccttcc
     1801 ttgtgtctgt cttttttcct tcagggacct ggaagtttca cttattcctt cctccccaca
     1861 attggctatg gcttctttac tgacagatca agaaccaatt gaggaacagg acagcatcag
     1921 aaccgcccct acagttagtg tttaactact tacagagcca ctttccctag gaccacttgg
     1981 cttctgttta gttagtattt cagcctcaat gagcatagga ttaagaaatt ttcaggtaca
     2041 attttgctcc tggaaatata tgtatagtga tgatttaaag tgggacagat ggtgttggtt
     2101 ctgtataata attttttgtc ttttaattgg gactttatag ataaattaat tagaatactg
     2161 agttctgtgc ataaaatgtt taggaccgta ttctataaca tgagtgtttt aaatctttcc
     2221 attaaaaatg tgtatttgtc aacactttta ctgtttcctc atcaaatctg cagaatcttt
     2281 tagtaggaat aattggtatt cggaggctaa cttcttaagc agcacaattt ctagttctag
     2341 caaaataggt ttcttgtttg cggagttgtt ctgtgttaag ttaaggactg tcttctgttt
     2401 actgcagagg gcagtgactc tgttgacacc agcttgattt ctccccattt gcatttattc
     2461 acaaatacaa actgaaaggg taaattatgt tcaaaggcct ttagagcaac agattcacac
     2521 gcacgcattt gactttcgac attcagcttg tagaataatg gagtctatcc agcaggctat
     2581 aaagcaagca cttgaaacaa aaccagaatg attaataacg gcaataactg aactatattt
     2641 ggagtgtttt gttacataat tgcagtcata atgagtacta cagaatctga ttatcttaca
     2701 gttgcaatct ggagtggctt tttatttcac ttggttataa aatgttatat gagagtgtga
     2761 tgtcacatat taatatagaa gatgcctacc taataagagt caattcttta ttaccaagac
     2821 cccaaaccat tcagagtggt ctctatttac acatatccca agaattttgt gtgtgcttct
     2881 atatttatac ttaggctttt ttaaaatcaa ttttcaatct taaatttttc tttagaaatg
     2941 cttcagttgg atccaaacat ttttccaact attaagaaaa atcacagtgg cagacatggc
     3001 actgcacacc tgtagtcaca gcattttggg ggctgaggtg gaagaattat aaattcaagt
     3061 cagccttggc tgcctagcaa gaccctcctc aaggatgatg tagggaggag agagggaagg
     3121 gggaaggcta gagcaggaga gaaaaatcac aaagcatact tgaaattgga aattagcttt
     3181 ttattttcat taagatttat ttatttttat cttattttat atgtataaat atcttggcaa
     3241 catgtatgtc tgcgtaccat gtgcatacct gttgcatggg gaagtcagaa ctggacatca
     3301 ggtcctccag aactgagata tatagatggt tttgaaccac catataggtg cttggaatca
     3361 aaagctcgtc ttctgtataa gcaacaagtg ctgttaactg ctgagctatc tctcaagccc
     3421 aaaagtagct taaaaaaatc acctgtggaa gcacaaaatt ccatggatcc agagtggaga
     3481 gtgttctgtt ctatgcatta agctatccag tacatacttt ttgttttgca catctgtgta
     3541 actggagtca aatgaatgac agtctaggaa aaatcatcta cctttgttct tgacaatatc
     3601 ttaaactata tctgaccagt gtgtttatag taaatgttta tctcttgggc ctatctaatg
     3661 tattcagtta atacagaaga ttaatgatag aaaaggtttt tttttttgtt ttgttttgtt
     3721 ttagttggaa gtagtgtttg tatttctgct tgaattcctg tctttggatt ctgtagtttt
     3781 aaaagtgctt ttgtataagt taaccacaat gtaaatcttc acagtacaca cacagttgtg
     3841 tgtgttgaat gtcattattt ttccagtata atgtcatgat acttcaaatg aacactaggt
     3901 gtggtattgg acttgattat cttgggattg atgaaaatat atgtcttaat gcatatattc
     3961 atcgacttta ttattaagat gattgtaaca aaacagcaaa catttattga gcattaaatg
     4021 cttgattgaa gtctt
//
