LOCUS       NM_145354               4280 bp    mRNA    linear   ROD 11-DEC-2024
DEFINITION  Mus musculus NOL1/NOP2/Sun domain family member 2 (Nsun2), mRNA.
ACCESSION   NM_145354
VERSION     NM_145354.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Xu,T., Gao,M., Zhang,L., Cao,T., Qiu,Y., Liu,S., Wu,W., Zhou,Y.,
            Liu,H., Zhang,R., Ruan,X. and Huang,J.
  TITLE     NSUN2 is critical for mouse oocyte meiosis and early embryonic
            development
  JOURNAL   Reproduction 168 (6), e240235 (2024)
   PUBMED   39231094
  REMARK    GeneRIF: NSUN2 is critical for mouse oocyte meiosis and early
            embryonic development.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Liu,L., Chen,Z., Zhang,K., Hao,H., Ma,L., Liu,H., Yu,B., Ding,S.,
            Zhang,X., Zhu,M., Guo,X., Liu,Y., Liu,H., Huang,F., Peng,K. and
            Guan,W.
  TITLE     NSUN2 mediates distinct pathways to regulate enterovirus 71
            replication
  JOURNAL   Virol Sin 39 (4), 574-586 (2024)
   PUBMED   38768712
  REMARK    GeneRIF: NSUN2 mediates distinct pathways to regulate enterovirus
            71 replication.
REFERENCE   3  (bases 1 to 4280)
  AUTHORS   Martinez-Mayer,J., Brinkmeier,M.L., O'Connell,S.P., Ukagwu,A.,
            Marti,M.A., Miras,M., Forclaz,M.V., Benzrihen,M.G., Cheung,L.Y.M.,
            Camper,S.A., Ellsworth,B.S., Raetzman,L.T., Perez-Millan,M.I. and
            Davis,S.W.
  TITLE     Knockout mice with pituitary malformations help identify human
            cases of hypopituitarism
  JOURNAL   Genome Med 16 (1), 75 (2024)
   PUBMED   38822427
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4280)
  AUTHORS   Zou,S., Huang,Y., Yang,Z., Zhang,J., Meng,M., Zhang,Y., Feng,J.,
            Sun,R., Li,W., Wang,W., Lopez,J.G. and Fang,L.
  TITLE     NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA
            stabilization
  JOURNAL   Clin Transl Med 14 (3), e1621 (2024)
   PUBMED   38468490
  REMARK    GeneRIF: NSUN2 promotes colorectal cancer progression by enhancing
            SKIL mRNA stabilization.
REFERENCE   5  (bases 1 to 4280)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 4280)
  AUTHORS   Khan,M.A., Rafiq,M.A., Noor,A., Hussain,S., Flores,J.V., Rupp,V.,
            Vincent,A.K., Malli,R., Ali,G., Khan,F.S., Ishak,G.E., Doherty,D.,
            Weksberg,R., Ayub,M., Windpassinger,C., Ibrahim,S., Frye,M.,
            Ansar,M. and Vincent,J.B.
  TITLE     Mutation in NSUN2, which encodes an RNA methyltransferase, causes
            autosomal-recessive intellectual disability
  JOURNAL   Am J Hum Genet 90 (5), 856-863 (2012)
   PUBMED   22541562
  REMARK    GeneRIF: NSUN2 localizes to the nucleolus of Purkinje cells in the
            cerebellum. Mutation to arginine at residue 679 causes NSUN2 to
            fail to localize within the nucleolus.
REFERENCE   7  (bases 1 to 4280)
  AUTHORS   Blanco,S., Kurowski,A., Nichols,J., Watt,F.M., Benitah,S.A. and
            Frye,M.
  TITLE     The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells
            to differentiate
  JOURNAL   PLoS Genet 7 (12), e1002403 (2011)
   PUBMED   22144916
  REMARK    GeneRIF: we identify that an RNA methyltransferase (Misu/Nsun2) is
            required to balance stem cell self-renewal and differentiation in
            skin
REFERENCE   8  (bases 1 to 4280)
  AUTHORS   Hussain,S., Benavente,S.B., Nascimento,E., Dragoni,I., Kurowski,A.,
            Gillich,A., Humphreys,P. and Frye,M.
  TITLE     The nucleolar RNA methyltransferase Misu (NSun2) is required for
            mitotic spindle stability
  JOURNAL   J Cell Biol 186 (1), 27-40 (2009)
   PUBMED   19596847
  REMARK    GeneRIF: These results suggest a novel mechanism by which c-Myc
            promotes proliferation by stabilizing the mitotic spindle in
            fast-dividing cells via Misu and NuSAP.
REFERENCE   9  (bases 1 to 4280)
  AUTHORS   Frye,M. and Watt,F.M.
  TITLE     The RNA methyltransferase Misu (NSun2) mediates Myc-induced
            proliferation and is upregulated in tumors
  JOURNAL   Curr Biol 16 (10), 971-981 (2006)
   PUBMED   16713953
  REMARK    GeneRIF: Misu is a novel downstream Myc target that methylates RNA
            polymerase III transcripts. Misu mediates Myc-induced cell
            proliferation and growth and is a potential target for cancer
            therapies.
            Erratum:[Curr Biol. 2007 Nov 20;17(22):2002]
REFERENCE   10 (bases 1 to 4280)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI787627.1, CJ092700.1, AK030124.1 and BX630130.1.
            
            On Nov 15, 2012 this sequence version replaced NM_145354.4.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus to one that is more supported by available conservation
            data. This update represents a more predominant splice pattern and
            extends the N-terminus by 66 aa, which is supported by orthologs in
            many other species, including human. There also is an upstream AUG,
            25aa upstream from the start site now used in this CCDS.  Based on
            transcript evidence the TSS is in or around this upstream ATG, so
            it would be unlikely that the ribosome could use it, as the
            ribosome generally needs
            20 nts of 5' UTR to bind and initiate correctly.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154789.1, SRR1660819.119769.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: lack of evidence for use of
                                                upstream AUG
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-388               AI787627.1         1-388
            389-655             CJ092700.1         388-654
            656-4157            AK030124.1         541-4042
            4158-4280           BX630130.1         2-124               c
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 35.55 cM"
     gene            1..4280
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /note="NOL1/NOP2/Sun domain family member 2"
                     /db_xref="GeneID:28114"
                     /db_xref="MGI:MGI:107252"
     exon            1..173
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..2351
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /EC_number="2.1.1.203"
                     /note="NOL1/NOP2/Sun domain family 2; tRNA
                     (cytosine-5-)-methyltransferase NSUN2; Myc-induced SUN
                     domain containing protein; tRNA
                     (cytosine(34)-C(5))-methyltransferase; myc-induced SUN
                     domain-containing protein; mRNA cytosine
                     C(5)-methyltransferase; tRNA cytosine
                     C(5)-methyltransferase"
                     /codon_start=1
                     /product="RNA cytosine C(5)-methyltransferase NSUN2"
                     /protein_id="NP_663329.3"
                     /db_xref="CCDS:CCDS36722.2"
                     /db_xref="GeneID:28114"
                     /db_xref="MGI:MGI:107252"
                     /translation="MGRRARGRRFQQPPQPEGEEDASDGGRKRGQAGWEGGYPEIVKE
                     NKLFEHYYQELKIVPEGEWDQFMESLREPLPATLRITGYKSHAKEILHCLKNKYFKEL
                     EDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPLLAKFHQFLVSETESGNISR
                     QEAVSMIPPLLLNVEPHHKILDMCAAPGSKTTQLIEMLHADMSVPFPEGFVIANDVDN
                     KRCYLLVHQAKRLSSPCIMVVNHDASSIPRLTVDVDGRKEILFYDRILCDVPCSGDGT
                     MRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPVEDEAVIAA
                     LLEKSEGALELADVSAELPGLKWMPGVSQWKVMTRDGQWFADWHEVPQGRHTQIRPTM
                     FPPTDLEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKAPMPWNKRQPKVQNKSAEAR
                     EPRVSSHVAATEGNPSDQSELESQMITGAGDSETAHNTENTESNEKKDGVCGPPPSKK
                     MKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKEL
                     RNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSME
                     DVKTLLTQENPFFRKLSSEAYSQVKDLAKGSVVLKYEPDSANPDTLQCPIVLCGWRGK
                     ASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNENAASPEQPGDEDAKQTAQDPC
                     VPDSVPGCDAAAAEPSR"
     exon            174..331
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            332..436
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            437..542
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            543..614
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            615..699
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            700..892
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            893..967
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1098
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1172
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1303
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1400
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1582
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1675
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1811
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1892
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2031
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2071
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..4280
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2811..2816
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /note="hexamer: AATAAA"
     polyA_site      2835
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /note="major polyA site"
     polyA_site      4163
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
     regulatory      4239..4244
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
                     /note="hexamer: AGTAAA"
     polyA_site      4261
                     /gene="Nsun2"
                     /gene_synonym="D13Wsu123e; Misu"
ORIGIN      
        1 ggatgcagcc ggaagtgcag cgtgcgtgcg gtttggtggg tcgctgtgtg cgctccgcgt
       61 gtgcagccgc gtgggccatg gggcggcggg cgcggggccg gcggttccag cagccgccgc
      121 agcctgaggg cgaggaagac gccagcgacg gcggcagaaa gcgaggccag gcgggctggg
      181 aaggtggcta tcccgagatc gtaaaggaga acaagctctt cgagcactac tatcaggaac
      241 tcaagatcgt gccagaggga gaatgggacc aattcatgga gtcactccga gaacctctcc
      301 cagccacact gagaatcact gggtacaaaa gccatgccaa agagattctc cattgcttga
      361 agaacaagta ctttaaggag ttggaggacc tggaagtaga tggacagaaa gttgaagttc
      421 cacaaccact aagctggtac cctgaagaac ttgcctggca tacaaactta agtcggaaaa
      481 tcttgaggaa gtccccgttg ttggcaaagt tccatcagtt cctggtcagc gagactgagt
      541 ctggaaacat cagccgccag gaggctgtca gcatgatccc cccactgctg ctcaacgtgg
      601 agccacacca taagatctta gacatgtgtg cagcccctgg atccaagacc acacagttaa
      661 ttgaaatgtt gcatgcagac atgagtgtgc cctttccaga gggatttgta atcgcaaatg
      721 acgtggacaa caagcgctgc tatctgctcg tccatcaggc caaaaggttg agcagtccct
      781 gcatcatggt ggtaaaccat gacgcatcca gcatacctag acttacagta gatgtggacg
      841 gaaggaaaga gattctcttc tatgatcgaa ttttatgtga tgtcccttgc agtggcgatg
      901 gcacaatgag aaaaaacatt gatgtctgga agaaatggac aaccttaaac agcttgcagc
      961 tccatggcct gcagcttcgg attgcaactc gaggtgctga gcagctggcg gaaggtggca
     1021 ggatggtgta ttccacgtgt tccttgaacc ccgtggagga tgaagcagtg atcgcagctc
     1081 tgctagagaa gagtgaagga gctcttgagc ttgctgatgt gtctgctgag ttgccaggac
     1141 tgaagtggat gcctggagtc tcacagtgga aggtcatgac tagagacggg cagtggtttg
     1201 cagactggca tgaggttccc cagggcaggc atacacaaat ccgacctacc atgttcccac
     1261 caacggacct ggagaagcta caggcaatgc atctagagcg atgccttcga atcctgcccc
     1321 atcatcagaa tactggaggg ttctttgtgg cagtattggt caagaaagca ccaatgccgt
     1381 ggaacaaacg tcagcccaag gtccagaata aatctgcaga agccagagaa cccagggtat
     1441 ccagccatgt ggctgccaca gagggaaatc ccagtgacca gtctgagctg gaaagtcaga
     1501 tgataactgg agctggtgac tcagaaacag ctcacaacac tgagaacaca gagagcaatg
     1561 agaagaaaga tggcgtgtgt ggccctcctc catcaaagaa aatgaagttg tttggattta
     1621 aagaagatcc atttgtattc attcctgaag atgatccttt atttccacct attgagaagt
     1681 tttatgcctt ggatccttca ttcccgagga tgaatctgtt aacccgaacc acagaaggaa
     1741 agaagcggca gctttatatg gtctccaagg agctgaggaa tgtactgctg aacaacagcg
     1801 agaagatgaa ggtcattaac actgggataa aagtctggtg tcgaaataac agtggtgaag
     1861 aattcgattg tgcattccgt ttggcacagg agggaatata tacattgtat ccatttatca
     1921 attcaagaat catcactgta tcaatggaag acgtgaagac actgttgacc caggagaacc
     1981 cattctttag aaaactgagc agtgaggcct acagtcaagt caaggacctt gcaaagggaa
     2041 gtgttgtgct gaagtatgag ccagattctg cgaatccaga caccctccag tgccccatcg
     2101 tgctgtgtgg gtggcgggga aaggcctcta ttcgaacttt tgtgcccaaa aatgagcggc
     2161 ttcattatct caggatgatg ggtctggagg tactgggaga aaagaagaaa gagggagtca
     2221 ttcttaccaa tgagaatgct gccagcccag agcagcctgg agatgaggat gccaagcaga
     2281 cagcacaaga cccctgcgtc ccagactccg tccctggctg tgatgcagct gcagctgagc
     2341 catcccggtg aaggagtctt cacacaatga attggtacca tccgttgttg gagatcaaac
     2401 ctagaatgtc cttagccagg gacctggaaa tgagtggtgg ccacagtctg ttaggagtcg
     2461 cttttgcaga gtggatacat gtttctgctg ttaaaggcta acagcctttg cagaagttca
     2521 gatcccttgt tggccagtat tgacctagtc ttctaaaacg acagtaaaag tctacaagtc
     2581 tttgggaagc agctctatct atctatgctt taatactttc tggactgcac accagtgtgc
     2641 tgtgcagtca tgctgacact gaggtctcag gtgacttctg tgcctttgtg ataaagggtg
     2701 agataagcca tctcagaggg aaggctggtg aatcatgaca gcagttttgg ggaccgtttg
     2761 tgcttatcct ggagttgttt taagtggggt ggagattgct tgggtcatga aataaagagc
     2821 tattatgatc atgtatcttg gcatgagact tggatttgct tttagttggc taatgggaga
     2881 gaaaagccag ccctctggtg tctcatacag gacttgaagc tggactgtca gccgtgtttc
     2941 cctcgcccag attctcccgg cagagtcctt gcaggagctc ttccttccct ctgtctgtaa
     3001 tgcattttgt ccgcacagtt gagcatcagt tacaccaatg ctcttccggc ctccctatat
     3061 gtgctctgag ctaaacaggc tacctctggg aactcagctg tgccttgctc ataaacgatc
     3121 agtccagctg gatttgatga ctgtttatat gccctgggga gggtcatagg agcctcacct
     3181 aaatgtccag ctgtgccatt cctcaagttg tatgtaactc ttccttgggg agggaccgga
     3241 ggatacaggc caaagaagac cagggtggga ttccatctct gtggctatgg gaaagctgtc
     3301 atctgctggt taaagacttt ggcactgcct ttgcagggta gggaaaccca agcctctgta
     3361 agtagcgctc acctgctcta caagtaagcc cagaaactca ttggttccca gaatgaattc
     3421 tggtagagct gtggagtgtg gatgaaacac tgttgggctc ttaggaggag gggcagactt
     3481 tgcttagttc cctgcccggg ggaggcattt tagcagcagt gggtctttcc tggcataggt
     3541 cttacacttg gacctgacct tgaggtgagt agatcaataa gccgtagcca ttgcaagtac
     3601 ctcactgtca agaagacggg tgtcagcctc agccagtgct acccaggctg cctccatctt
     3661 gtgactcctc ctcttacagg gtgcgggtta gttactttgt aaaatatttt tgggctttta
     3721 agtctaacta gagcagatag gcagtgcagc tagggcttgt gttaccattg gttgtcacct
     3781 cgtggtgaca gctttgcttg ttggcaggca ggtgtctgcc attgtcttcg tccttatttg
     3841 tgtcttacag ggaggtctcc taagacttgg ttctttttcc tcatcagact tcaacagcat
     3901 cagcattgtt ttttgagaca tgattttgtt aagttttgac cctgagaacc tcctgcttca
     3961 gcctcctagg tactgggctt ctcagcatga gttgccatgc cagctttgga tgccgtcctg
     4021 tagagacccc cttagcagat gaaacttact ctgattcttg gtcaaatcct actgggagga
     4081 caggccgggt ggcaatcctg ggtgccattc atgctgtgtg cttttccctt gggttccaga
     4141 atattttaaa tggttccaat gaatgcttat agtgaagtgt gttttctttt caatttcagt
     4201 acggtttgtc tgattcccat aaggtgctgc tctgtggaag taaagacatt tgcctatagg
     4261 acttcttggc cttgcataaa
//
