LOCUS       NM_198326               1486 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus NSFL1 (p97) cofactor (p47) (Nsfl1c), transcript
            variant 1, mRNA.
ACCESSION   NM_198326 XM_972636
VERSION     NM_198326.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Liu,F., He,R., Xu,X., Zhu,M., Yu,H. and Liu,Y.
  TITLE     Munc18c accelerates SNARE-dependent membrane fusion in the presence
            of regulatory proteins alpha-SNAP and NSF
  JOURNAL   J Biol Chem 300 (3), 105782 (2024)
   PUBMED   38395304
  REMARK    GeneRIF: Munc18c accelerates SNARE-dependent membrane fusion in the
            presence of regulatory proteins alpha-SNAP and NSF.
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Al Ghouleh,I., Meijles,D.N., Mutchler,S., Zhang,Q., Sahoo,S.,
            Gorelova,A., Henrich Amaral,J., Rodriguez,A.I., Mamonova,T.,
            Song,G.J., Bisello,A., Friedman,P.A., Cifuentes-Pagano,M.E. and
            Pagano,P.J.
  TITLE     Binding of EBP50 to Nox organizing subunit p47phox is pivotal to
            cellular reactive species generation and altered vascular phenotype
  JOURNAL   Proc Natl Acad Sci U S A 113 (36), E5308-E5317 (2016)
   PUBMED   27540115
  REMARK    GeneRIF: our data identify EBP50 as a previously unidentified
            regulator of Nox1 and support that it promotes Nox1 activity by
            binding p47(phox) This interaction is pivotal for agonist-induced
            smooth muscle ROS, hypertrophy, and vasoconstriction and has
            implications for ROS-mediated physiological and pathophysiological
            processes.
REFERENCE   3  (bases 1 to 1486)
  AUTHORS   Koulajian,K., Desai,T., Liu,G.C., Ivovic,A., Patterson,J.N.,
            Tang,C., El-Benna,J., Joseph,J.W., Scholey,J.W. and Giacca,A.
  TITLE     NADPH oxidase inhibition prevents beta cell dysfunction induced by
            prolonged elevation of oleate in rodents
  JOURNAL   Diabetologia 56 (5), 1078-1087 (2013)
   PUBMED   23429921
  REMARK    GeneRIF: Data suggest that NADPH oxidase-derived cytosolic
            superoxide is increased in islets upon oleate exposure and leads to
            beta cell dysfunction; whole-body NADPH oxidase inhibition (via p47
            knockout) decreases superoxide and restores islet function.
REFERENCE   4  (bases 1 to 1486)
  AUTHORS   Lo,J.C., Jamsai,D., O'Connor,A.E., Borg,C., Clark,B.J.,
            Whisstock,J.C., Field,M.C., Adams,V., Ishikawa,T., Aitken,R.J.,
            Whittle,B., Goodnow,C.C., Ormandy,C.J. and O'Bryan,M.K.
  TITLE     RAB-like 2 has an essential role in male fertility, sperm
            intra-flagellar transport, and tail assembly
  JOURNAL   PLoS Genet 8 (10), e1002969 (2012)
   PUBMED   23055941
REFERENCE   5  (bases 1 to 1486)
  AUTHORS   Delano,M.J., Thayer,T., Gabrilovich,S., Kelly-Scumpia,K.M.,
            Winfield,R.D., Scumpia,P.O., Cuenca,A.G., Warner,E., Wallet,S.M.,
            Wallet,M.A., O'Malley,K.A., Ramphal,R., Clare-Salzer,M.,
            Efron,P.A., Mathews,C.E. and Moldawer,L.L.
  TITLE     Sepsis induces early alterations in innate immunity that impact
            mortality to secondary infection
  JOURNAL   J Immunol 186 (1), 195-202 (2011)
   PUBMED   21106855
REFERENCE   6  (bases 1 to 1486)
  AUTHORS   Yao,H., Edirisinghe,I., Yang,S.R., Rajendrasozhan,S., Kode,A.,
            Caito,S., Adenuga,D. and Rahman,I.
  TITLE     Genetic ablation of NADPH oxidase enhances susceptibility to
            cigarette smoke-induced lung inflammation and emphysema in mice
  JOURNAL   Am J Pathol 172 (5), 1222-1237 (2008)
   PUBMED   18403597
  REMARK    GeneRIF: genetic ablation of components of NADPH oxidase enhances
            susceptibility to the proinflammatory effects of CS leading to
            airspace enlargement and alveolar damage.
REFERENCE   7  (bases 1 to 1486)
  AUTHORS   Villen,J., Beausoleil,S.A., Gerber,S.A. and Gygi,S.P.
  TITLE     Large-scale phosphorylation analysis of mouse liver
  JOURNAL   Proc Natl Acad Sci U S A 104 (5), 1488-1493 (2007)
   PUBMED   17242355
REFERENCE   8  (bases 1 to 1486)
  AUTHORS   Dreveny,I., Kondo,H., Uchiyama,K., Shaw,A., Zhang,X. and
            Freemont,P.S.
  TITLE     Structural basis of the interaction between the AAA ATPase p97/VCP
            and its adaptor protein p47
  JOURNAL   EMBO J 23 (5), 1030-1039 (2004)
   PUBMED   14988733
  REMARK    GeneRIF: that p47 ubiquitin regulatory X domain domains may act as
            general p97/valosin containing protein/CDC48 binding modules and
            that adaptor binding for N-ethylmaleimide sensitive factor and p97
            might involve different binding sites
REFERENCE   9  (bases 1 to 1486)
  AUTHORS   Meyer,H.H., Shorter,J.G., Seemann,J., Pappin,D. and Warren,G.
  TITLE     A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to
            ubiquitin and nuclear transport pathways
  JOURNAL   EMBO J 19 (10), 2181-2192 (2000)
   PUBMED   10811609
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ152490.1, AK137088.1 and AL928719.6.
            
            On Mar 22, 2014 this sequence version replaced NM_198326.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050936.1, AK137088.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                CJ152490.1         2-100
            100-1478            AK137088.1         2-1380
            1479-1486           AL928719.6         74047-74054
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 74.83 cM"
     gene            1..1486
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /note="NSFL1 (p97) cofactor (p47)"
                     /db_xref="GeneID:386649"
                     /db_xref="MGI:MGI:3042273"
     exon            1..231
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..1245
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /note="isoform a is encoded by transcript variant 1; NSFL1
                     cofactor p47; p97 cofactor p47"
                     /codon_start=1
                     /product="NSFL1 cofactor p47 isoform a"
                     /protein_id="NP_938085.1"
                     /db_xref="CCDS:CCDS16868.1"
                     /db_xref="GeneID:386649"
                     /db_xref="MGI:MGI:3042273"
                     /translation="MAEERQDALREFVAVTGTEEDRARFFLESAGWDLQIALASFYED
                     GGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEEEEEEEGQRSRFYAGGSE
                     RSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLG
                     AAPEEESAYVAGERRRHSGQDVHVVLKLWKTGFSLDNGDLRSYQDPSNAQFLESIRRG
                     EVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLNTSSPA
                     QQAENEAKASSSILINEAEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAM
                     AATSFVLMTTFPNKELADENQTLKEANLLNAVIVQRLT"
     exon            232..329
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     exon            330..404
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     exon            405..410
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     exon            411..576
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     exon            577..669
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     exon            670..779
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     exon            780..917
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1082
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1486
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1455..1460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /note="hexamer: AATAAA"
     polyA_site      1477
                     /gene="Nsfl1c"
                     /gene_synonym="Munc-18c; p47; Stxbp3a"
                     /note="major polyA site"
ORIGIN      
        1 attgggcgga gcctacgagc aggcgcccag cggctggacg cggtagaacg gcgggcggaa
       61 cgcagacctg gggttcgggc tgttgcagtg tccgccggag cggtttggga agtgcacggg
      121 gcgaagatgg cggaggagcg gcaggacgcg ttgagggagt tcgtggcggt gactggcact
      181 gaggaggaca gggcccgttt cttcctggag tcggctggct gggacctgca gattgcactt
      241 gcaagctttt atgaggatgg aggggatgaa gacattgtga ccatttcaca agcaacccca
      301 agttcagtgt ccagaggcac agctcctagt gataatagag tgacatcctt cagagacctc
      361 attcatgacc aagatgaaga ggaggaggaa gaggagggac agaggagcag gttttatgct
      421 gggggctcag agagaagtgg acagcagatt gttggtcctc cccggaagaa aagtcccaac
      481 gagctggtgg atgatctgtt taagggtgcc aaagagcatg gggctgtagc tgtggaacga
      541 gtgaccaaga gccctggaga gaccagtaaa ccgagaccat ttgcaggagg gggctaccgc
      601 cttggagcag caccagagga agagtctgcc tatgtggcag gagaaaggag gcggcattca
      661 ggccaggatg ttcatgtagt gctaaagctc tggaaaactg gattcagcct agacaatggt
      721 gaccttagaa gctaccaaga cccatccaat gcccagtttc tggagtctat ccgtagaggg
      781 gaggttcctg cagagcttcg gaggttagcg catggtgggc aggtgaacct ggatatggag
      841 gatcatcggg atgaggactt cgtgaagccc aagggagctt tcaaagcctt cactggcgag
      901 ggccagaaac tgggcagcac ggccccccag gtattaaaca ccagctctcc agcccaacaa
      961 gcagaaaatg aagccaaagc tagctcgtcc atcttaatca atgaagcaga acctaccacg
     1021 aacatccaaa tccggcttgc agatggcggg aggctggtgc agaaattcaa ccacagccac
     1081 aggatcagcg acatccggct cttcatcgtg gatgcccggc cagctatggc tgccaccagt
     1141 tttgtcctta tgactacctt ccctaacaaa gagctggctg atgagaacca aaccctgaag
     1201 gaagccaacc ttctcaacgc tgtcatcgtg cagcggttaa cataaccgcc cagctgttgc
     1261 ctcacctccc taagtgtctc ccatgactgt ggccatgccc gtggggattt cctcccaccc
     1321 cgtacacacc tagcccttcc agtgccacgt ctcgtccgta gctctggatt cttagacttc
     1381 agttggacat ttgttttctc tttagttgta tttcctgggt ttttgtgatg atcaatggac
     1441 tttaaagaaa aaaaaataaa aaacacaaaa atttgaagaa atacta
//
