LOCUS       NM_008740               3766 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Mus musculus N-ethylmaleimide sensitive fusion protein (Nsf), mRNA.
ACCESSION   NM_008740
VERSION     NM_008740.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Liu,F., He,R., Xu,X., Zhu,M., Yu,H. and Liu,Y.
  TITLE     Munc18c accelerates SNARE-dependent membrane fusion in the presence
            of regulatory proteins alpha-SNAP and NSF
  JOURNAL   J Biol Chem 300 (3), 105782 (2024)
   PUBMED   38395304
  REMARK    GeneRIF: Munc18c accelerates SNARE-dependent membrane fusion in the
            presence of regulatory proteins alpha-SNAP and NSF.
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3766)
  AUTHORS   Bustamante-Barrientos,F.A., Mendez-Ruette,M., Molina,L., Koning,T.,
            Ehrenfeld,P., Gonzalez,C.B., Wyneken,U., Henzi,R. and Batiz,L.F.
  TITLE     Alpha-SNAP (M105I) mutation promotes neuronal differentiation of
            neural stem/progenitor cells through overactivation of AMPK
  JOURNAL   Front Cell Dev Biol 11, 1061777 (2023)
   PUBMED   37113766
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3766)
  AUTHORS   Xie,M.J., Iwata,K., Ishikawa,Y., Nomura,Y., Tani,T., Murata,K.,
            Fukazawa,Y. and Matsuzaki,H.
  TITLE     Autistic-Like Behavior and Impairment of Serotonin Transporter and
            AMPA Receptor Trafficking in N-Ethylmaleimide Sensitive Factor
            Gene-Deficient Mice
  JOURNAL   Front Genet 12, 748627 (2021)
   PUBMED   34745222
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3766)
  AUTHORS   Umanah,G.K.E., Ghasemi,M., Yin,X., Chang,M., Kim,J.W., Zhang,J.,
            Ma,E., Scarffe,L.A., Lee,Y.I., Chen,R., Tangella,K., McNamara,A.,
            Abalde-Atristain,L., Dar,M.A., Bennett,S., Cortes,M., Andrabi,S.A.,
            Doulias,P.T., Ischiropoulos,H., Dawson,T.M. and Dawson,V.L.
  TITLE     AMPA Receptor Surface Expression Is Regulated by S-Nitrosylation of
            Thorase and Transnitrosylation of NSF
  JOURNAL   Cell Rep 33 (5), 108329 (2020)
   PUBMED   33147468
  REMARK    GeneRIF: AMPA Receptor Surface Expression Is Regulated by
            S-Nitrosylation of Thorase and Transnitrosylation of NSF.
REFERENCE   6  (bases 1 to 3766)
  AUTHORS   Gerst,J.E.
  TITLE     SNAREs and SNARE regulators in membrane fusion and exocytosis
  JOURNAL   Cell Mol Life Sci 55 (5), 707-734 (1999)
   PUBMED   10379359
  REMARK    Review article
REFERENCE   7  (bases 1 to 3766)
  AUTHORS   Woodman,P.G.
  TITLE     The roles of NSF, SNAPs and SNAREs during membrane fusion
  JOURNAL   Biochim Biophys Acta 1357 (2), 155-172 (1997)
   PUBMED   9223620
  REMARK    Review article
REFERENCE   8  (bases 1 to 3766)
  AUTHORS   Hoyle,J., Phelan,J.P., Bermingham,N. and Fisher,E.M.
  TITLE     Localization of human and mouse N-ethylmaleimide-sensitive factor
            (NSF) gene: a two-domain member of the AAA family that is involved
            in membrane fusion
  JOURNAL   Mamm Genome 7 (11), 850-852 (1996)
   PUBMED   8875895
REFERENCE   9  (bases 1 to 3766)
  AUTHORS   Perier,F., Coulter,K.L., Liang,H., Radeke,C.M., Gaber,R.F. and
            Vandenberg,C.A.
  TITLE     Identification of a novel mammalian member of the
            NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in
            yeast
  JOURNAL   FEBS Lett 351 (2), 286-290 (1994)
   PUBMED   8082782
REFERENCE   10 (bases 1 to 3766)
  AUTHORS   Puschel,A.W., O'Connor,V. and Betz,H.
  TITLE     The N-ethylmaleimide-sensitive fusion protein (NSF) is
            preferentially expressed in the nervous system
  JOURNAL   FEBS Lett 347 (1), 55-58 (1994)
   PUBMED   8013662
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL603829.5 and AL596108.13.
            
            On Apr 27, 2007 this sequence version replaced NM_008740.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006627.1, AK049281.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                AL603829.5         29045-29131         c
            88-173              AL603829.5         5805-5890           c
            174-273             AL603829.5         3819-3918           c
            274-313             AL596108.13        200189-200228       c
            314-480             AL596108.13        190547-190713       c
            481-588             AL596108.13        187842-187949       c
            589-664             AL596108.13        187357-187432       c
            665-820             AL596108.13        184484-184639       c
            821-1020            AL596108.13        156783-156982       c
            1021-1186           AL596108.13        147186-147351       c
            1187-1261           AL596108.13        146632-146706       c
            1262-1449           AL596108.13        146200-146387       c
            1450-1545           AL596108.13        137280-137375       c
            1546-1702           AL596108.13        135934-136090       c
            1703-1836           AL596108.13        132936-133069       c
            1837-1903           AL596108.13        122035-122101       c
            1904-1983           AL596108.13        120415-120494       c
            1984-2118           AL596108.13        102473-102607       c
            2119-2232           AL596108.13        101252-101365       c
            2233-2288           AL596108.13        97780-97835         c
            2289-3766           AL596108.13        95823-97300         c
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 67.54 cM"
     gene            1..3766
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /note="N-ethylmaleimide sensitive fusion protein"
                     /db_xref="GeneID:18195"
                     /db_xref="MGI:MGI:104560"
     exon            1..87
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..2310
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /EC_number="3.6.4.6"
                     /note="NEM-sensitive fusion protein; vesicular-fusion
                     protein NSF; suppressor of K(+) transport growth defect 2;
                     N-ethylmaleimide sensitive factor"
                     /codon_start=1
                     /product="vesicle-fusing ATPase"
                     /protein_id="NP_032766.2"
                     /db_xref="CCDS:CCDS25524.1"
                     /db_xref="GeneID:18195"
                     /db_xref="MGI:MGI:104560"
                     /translation="MAGRTMQAARCPTDELSLSNCAVVNEKDFQSGQHVMVRTSPNHK
                     YIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFL
                     QKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSIL
                     KGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKM
                     GIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKM
                     LNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAIC
                     KQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVK
                     MEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQS
                     TAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGII
                     KWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIK
                     ICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQ
                     ALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALEL
                     LGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALMREEGASP
                     LDFD"
     exon            88..173
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            174..273
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            274..313
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            314..480
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            481..588
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            589..664
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            665..820
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            821..1020
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1186
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1261
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1449
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1545
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1702
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1836
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1903
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..1983
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2118
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2232
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2288
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2289..3766
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3754..3759
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /note="hexamer: AATATA"
     polyA_site      3766
                     /gene="Nsf"
                     /gene_synonym="SKD2"
                     /note="major polyA site"
ORIGIN      
        1 gcgagcacgc gcgcggcgga ggcgggctca gcagagtgcg gagctgcaac ctgggagccg
       61 gacgtgtccg cgaagatggc gggccggact atgcaagctg cgagatgccc tactgatgaa
      121 ttatctttaa gcaactgtgc ggttgtgaac gaaaaagatt tccagtctgg ccagcatgtg
      181 atggtgagga cgtcccccaa tcacaagtac atatttacgc tgaggaccca tccgtcagtg
      241 gttccaggat gcatcgcgtt cagcttgcct cagcgaaaat gggctggact ttctattgga
      301 caggacatag aagttgcctt gtattcgttt gacaaagcca agcagtgcat cggcacaatg
      361 accatcgaga tcgacttcct gcagaagaag aacatcgact ccaaccctta cgacaccgac
      421 aagatggccg ccgagttcat ccagcagttc aacaaccagg ccttctcagt ggggcagcag
      481 cttgtcttta gcttcaatga taagctcttt ggattactgg tgaaggacat tgaagccatg
      541 gatcccagca tcctgaaggg agagcctgca tcaggcaaaa ggcagaagat tgaggtaggc
      601 ctggttgttg gaaacagtca agttgcattt gaaaaagcag aaaattcgtc gctcaatctt
      661 atcggcaaag ctaaaaccaa ggaaaatcgc cagtccatca tcaatcccga ctggaacttt
      721 gagaaaatgg ggatcggcgg tctggataag gagttctcgg acattttccg aagagcattt
      781 gcttctcggg tgttcccccc ggagatcgtg gaacagatgg gttgcaaaca tgttaaaggc
      841 atcctgttat acggaccccc tggttgtggt aaaactctct tggctcgaca gattggcaag
      901 atgctgaatg cgagagagcc caaggtggtc aatgggccag aaatccttaa caagtatgtg
      961 ggagaatcag aggctaacat tcgtaaactc tttgctgatg ccgaagagga gcaaaggagg
     1021 cttggtgcta acagtggctt gcacatcatc atctttgatg aaatcgatgc catctgcaag
     1081 cagagaggga gcatggccgg cagcactgga gtgcatgaca ccgtggtcaa ccagctgctg
     1141 tccaagatcg atggcgttga gcagctgaac aacatcctcg ttatcggaat gaccaataga
     1201 ccagatttga tagacgaagc tctccttcga cccggaagac tggaagttaa aatggagata
     1261 ggcttgccag atgagaaggg tcgactccag atccttcata tccacacagc aaggatgaga
     1321 gggcaccagt tactgtctgc agatgtggac atcaaggagc tggctgtgga gactaagaat
     1381 ttcagtggcg ctgagctgga gggtctggtg cgagcagcgc agtccacagc catgaacaga
     1441 catatcaagg ccagcaccaa agtagaagta gacatggaga aggcagagag cctgcaggtg
     1501 acaagagggg acttccttgc ttccctggag aacgatatca aaccagcatt tggcaccaac
     1561 caagaggatt atgcaagtta cattatgaat gggatcatca agtggggtga ccccgtgacc
     1621 cgagtcctag atgacggaga gctgctggtc cagcaaacga aaaacagtga ccggacacca
     1681 ctcgttagtg tcctcctaga agggcctcct cacagcggga agactgccct ggctgcaaag
     1741 attgccgagg aatccaactt ccccttcatc aagatctgct cacctgataa gatgattggc
     1801 ttctccgaga cagccaagtg tcaggccatg aagaagatct ttgatgatgc ttacaaatct
     1861 cagctcagct gtgtggtcgt ggatgacatt gagaggctgc tcgattatgt ccccattggt
     1921 ccccggttct ccaacctggt attgcaggct cttcttgtgt tactgaaaaa ggcgcctcct
     1981 cagggccgca agcttcttat catcgggacc accagccgca aagatgtcct gcaggaaatg
     2041 gagatgctga acgccttcag caccaccatc cacgtgccca acattgccac aggagagcag
     2101 ctgctggaag ctctggagct tttgggcaac ttcaaagata aggaacgcac cacaattgct
     2161 cagcaagtca aagggaagaa agtctggata ggaatcaaga agttactaat gttgattgag
     2221 atgtccctgc agatggatcc tgagtaccgt gtgaggaaat tcttggccct catgagagaa
     2281 gaaggcgcta gtcccctgga ctttgattga aaacggactg tttacaacag tgaccaaggg
     2341 aagcgaccaa ggtgcagatg gcctgggatc gtcactcctc acactcaaga cactggacta
     2401 cgtgaagtgt ccgcctctct ccacacactc gctctgcatg actagtgcag tgaactccct
     2461 cgtgcttttg agatgttctc gtgttccctg gactgctggg cttccctccc gtgctagcta
     2521 agatggttcc tgcttatgtg ggaggcatgt cacacccgtg cacgctgccc acaccgcgat
     2581 ccctaccacc tgcccgaagg tcaacttcta catgagacca agagcaaagg tagaggttcc
     2641 aagggagtcc cttggcgctc cccatgctaa cagctgtgta tcccgcagat taggtcattg
     2701 tctgttccta aagaactagg ctttgaatat ctaagatgct gttctcaatc attttcagag
     2761 atatttccaa gttgcaatca ggagagcttt tctaatcgga agtcaaatca ttcctggggt
     2821 ggctgtgata tgctctgagt gtaattaatt tgctctgtgg taagagatat gctaatcatt
     2881 gccacttggt agatgccgtt aaaaccttat gaaagataca tatggaaatc tgtacatccc
     2941 taaacagaaa aaaaattaaa ataatttaca agcctctcaa ttctaattag cgtccggctc
     3001 taagtgggtc agttccttag gatgacgtta gaggcctatt agtgccatcg tcttcctttt
     3061 cctcagcctg atgacttaca attcatcctc cttgtatctc aagtgatctg tagtgtctgc
     3121 agcacataaa taaaacacta ctctccagca gaacaagcca ccccctagtc ctctccctca
     3181 gccctcgaat atccattcca gcgtactcaa cacacctctt cccgaagagg aactcagcga
     3241 gccctttgga gggtggtttg cctaatcagt gggttagaag gaatgcaatt aggtcgtcac
     3301 gtcattcgtg gagatgagtc ttagggcgca tgccactctc cccgctgctg cttcaaacac
     3361 aaagacagcc atcagaagcc gcccgggagg cttgctgcca caggggcacc tgggtgatac
     3421 tctgacactg tccccacagt agaaggcaaa tcatctccta gtcttgccgt ctgtatacct
     3481 tgtttggggt tttcatccct ggcttatttt gtggctttga aagagtgaat tgtgttgtga
     3541 aactgtgatt cccgtggcta gtaccgtggt tccctgtcca ccaccgtggt ccctgttgtc
     3601 tgcagttctg cctttctcct cccaacaaag tcctacatga attaatttat gtttcttgtt
     3661 caacgaagtt aatgtcttgt gtttttgtcc aaaattatat tgaaaaagat attatattgt
     3721 gtaatgcaaa ttgaaggaat gcaataaaga gtaaatatac ttgaaa
//
