LOCUS       NM_001081269            9652 bp    mRNA    linear   ROD 24-OCT-2024
DEFINITION  Mus musculus nuclear receptor binding SET domain protein 3 (Nsd3),
            transcript variant 2, mRNA.
ACCESSION   NM_001081269 XM_620640
VERSION     NM_001081269.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Shipman,G.A., Padilla,R., Horth,C., Hu,B., Bareke,E.,
            Vitorino,F.N., Gongora,J.M., Garcia,B.A., Lu,C. and Majewski,J.
  TITLE     Systematic perturbations of SETD2, NSD1, NSD2, NSD3, and ASH1L
            reveal their distinct contributions to H3K36 methylation
  JOURNAL   Genome Biol 25 (1), 263 (2024)
   PUBMED   39390582
  REMARK    GeneRIF: Systematic perturbations of SETD2, NSD1, NSD2, NSD3, and
            ASH1L reveal their distinct contributions to H3K36 methylation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 9652)
  AUTHORS   Yao,Y., Gu,J., Li,M., Li,G., Ai,J. and Zhao,L.
  TITLE     WHSC1L1-mediated epigenetic downregulation of VMP1 participates in
            herpes simplex virus 1 infection-induced mitophagy impairment and
            neuroinflammation
  JOURNAL   Mol Immunol 163, 63-74 (2023)
   PUBMED   37748280
  REMARK    GeneRIF: WHSC1L1-mediated epigenetic downregulation of VMP1
            participates in herpes simplex virus 1 infection-induced mitophagy
            impairment and neuroinflammation.
REFERENCE   4  (bases 1 to 9652)
  AUTHORS   Yuan,G., Flores,N.M., Hausmann,S., Lofgren,S.M., Kharchenko,V.,
            Angulo-Ibanez,M., Sengupta,D., Lu,X., Czaban,I., Azhibek,D.,
            Vicent,S., Fischle,W., Jaremko,M., Fang,B., Wistuba,I.I.,
            Chua,K.F., Roth,J.A., Minna,J.D., Shao,N.Y., Jaremko,L., Mazur,P.K.
            and Gozani,O.
  TITLE     Elevated NSD3 histone methylation activity drives squamous cell
            lung cancer
  JOURNAL   Nature 590 (7846), 504-508 (2021)
   PUBMED   33536620
REFERENCE   5  (bases 1 to 9652)
  AUTHORS   Wang,C., Wang,Q., Xu,X., Xie,B., Zhao,Y., Li,N. and Cao,X.
  TITLE     The methyltransferase NSD3 promotes antiviral innate immunity via
            direct lysine methylation of IRF3
  JOURNAL   J Exp Med 214 (12), 3597-3610 (2017)
   PUBMED   29101251
  REMARK    GeneRIF: This study reveals a critical role of NSD3-mediated IRF3
            methylation in enhancing antiviral innate immunity.
REFERENCE   6  (bases 1 to 9652)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 9652)
  AUTHORS   Kim,S.M., Kim,J.Y., Choe,N.W., Cho,I.H., Kim,J.R., Kim,D.W.,
            Seol,J.E., Lee,S.E., Kook,H., Nam,K.I., Kook,H., Bhak,Y.Y. and
            Seo,S.B.
  TITLE     Regulation of mouse steroidogenesis by WHISTLE and JMJD1C through
            histone methylation balance
  JOURNAL   Nucleic Acids Res 38 (19), 6389-6403 (2010)
   PUBMED   20530532
REFERENCE   8  (bases 1 to 9652)
  AUTHORS   Kim,S.M., Kee,H.J., Choe,N., Kim,J.Y., Kook,H., Kook,H. and
            Seo,S.B.
  TITLE     The histone methyltransferase activity of WHISTLE is important for
            the induction of apoptosis and HDAC1-mediated transcriptional
            repression
  JOURNAL   Exp Cell Res 313 (5), 975-983 (2007)
   PUBMED   17239852
REFERENCE   9  (bases 1 to 9652)
  AUTHORS   Kim,S.M., Kee,H.J., Eom,G.H., Choe,N.W., Kim,J.Y., Kim,Y.S.,
            Kim,S.K., Kook,H., Kook,H. and Seo,S.B.
  TITLE     Characterization of a novel WHSC1-associated SET domain protein
            with H3K4 and H3K27 methyltransferase activity
  JOURNAL   Biochem Biophys Res Commun 345 (1), 318-323 (2006)
   PUBMED   16682010
REFERENCE   10 (bases 1 to 9652)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC156990.11 and AC162367.5.
            
            [WARNING] On Mar 21, 2025 this sequence was replaced by
            NM_001081269.3.
            
            On May 8, 2015 this sequence version replaced NM_001081269.1.
            
            Summary: This gene encodes a member of the SET domain family of
            histone lysine N-methyltransferase proteins. This protein
            methylates histone H3 at lysine residues 4 and 27, which represses
            gene transcription. It acts in opposition to the histone
            demethylase Jmjd1c. Alternative splicing results in multiple
            transcript variants encoding different isoforms. [provided by
            RefSeq, May 2015].
            
            Transcript Variant: This variant (2) has a shorter 5' UTR, lacks
            the 3' terminal exon, and contains multiple alternate 3' exons,
            resulting in a distinct 3' coding region and 3' UTR, compared to
            variant 1. The encoded isoform (2) has a distinct C-terminus and is
            longer than isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-749               AC156990.11        61905-62653
            750-821             AC156990.11        69769-69840
            822-984             AC156990.11        70869-71031
            985-1139            AC156990.11        81069-81223
            1140-1655           AC156990.11        84069-84584
            1656-1782           AC156990.11        87358-87484
            1783-1883           AC156990.11        94662-94762
            1884-1929           AC162367.5         126252-126297       c
            1930-2060           AC162367.5         123148-123278       c
            2061-2189           AC162367.5         122000-122128       c
            2190-2316           AC162367.5         121220-121346       c
            2317-2514           AC162367.5         119298-119495       c
            2515-2685           AC162367.5         118672-118842       c
            2686-2832           AC162367.5         110822-110968       c
            2833-2989           AC162367.5         107138-107294       c
            2990-3192           AC162367.5         103047-103249       c
            3193-3305           AC162367.5         101959-102071       c
            3306-3575           AC162367.5         101262-101531       c
            3576-3692           AC162367.5         95342-95458         c
            3693-3834           AC162367.5         92779-92920         c
            3835-3941           AC162367.5         91204-91310         c
            3942-4143           AC162367.5         88424-88625         c
            4144-9652           AC162367.5         82316-87824         c
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 14.17 cM"
     gene            1..9652
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /note="nuclear receptor binding SET domain protein 3"
                     /db_xref="GeneID:234135"
                     /db_xref="MGI:MGI:2142581"
     exon            1..749
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..4385
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /EC_number="2.1.1.370"
                     /EC_number="2.1.1.371"
                     /note="isoform 2 is encoded by transcript variant 2;
                     histone-lysine N-methyltransferase NSD3; WHSC1-like
                     protein 1; nuclear SET domain-containing protein 3;
                     wolf-Hirschhorn syndrome candidate 1-like protein 1
                     homolog"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase NSD3 isoform
                     2"
                     /protein_id="NP_001074738.1"
                     /db_xref="CCDS:CCDS52528.1"
                     /db_xref="GeneID:234135"
                     /db_xref="MGI:MGI:2142581"
                     /translation="MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNHSDIVEDG
                     GPTPFEATLQQGFQYPPTTEDLPPLTNGYPPSISLYETQTKYPPYNQYPNGSANGFGA
                     VRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPLPPPPPPPPPPPSVPQTVIPKKTGS
                     PEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNRHESSRS
                     EERRSHKIPKLEPEGQNRPNERVDTAPEKPREEPVLKEAIPVQPILSSVPTTETSTGV
                     KFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHSKINTRGAREYHVQFFSNQPERAWVHE
                     KRVREYKGHEQYEELLAEAAKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMT
                     REERVEQYTFIYIDKQPEEASSQAKKNVTSKTEVKKPRRPRSVLNSQPEQTNAGEVAS
                     SQSSTDLRRQSQRRHTSLEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMS
                     PVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISSPSQRSEKPA
                     QSASSPEATSGSAGPVEKKQQRRSIRTRSESEKSAEVVPKKKIKKEQVETAPQASLKT
                     GLQKGASEISDSCKPLKKRSRASTDVETASCTYRDTSDSDSRGLSDGQVGFGKQVDSP
                     SATADADASDAQSVDSSLSRRGVGTSKKDTVCQVCEKAGDCLVACEGECCRHFHVECL
                     GLTAVPEGHFTCEECETGQHPCFSCKVSGKDVKRCSVSVCGKFYHEACVRKFPTAIFE
                     SKGFRCPQHCCSSCSMEKDIHKASKGRMMRCLRCPVAYHVGDACVAAGSVSVSSHILI
                     CSNHSKRSSQSAAINVGFCFVCARGLIVQDHSDPMFSSYAYKSHYLLSESNRAELMKL
                     PMIPSSSASKKRCEKGGRLLCCESCPASFHPECLSIDMPEGCWNCNDCKAGKKLHYKQ
                     IVWVKLGNYRWWPAEICSPRSVPLNIQGLKHDLGDFPVFFFGSHDYYWVHQGRVFPYV
                     EGDKHFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEMERTSRKPPPYKHIKA
                     NKVIGKVQVQVADLSEIPRCNCKPGDENPCGLESQCLNRMSQYECHPQVCPAGDRCQN
                     QCFTKRLYPDAEVIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHEN
                     SVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIP
                     AGMELTFNYNLDCLGNGRTVCHCGADNCSGFLGVRPKSACTSAVDEKTKNAKLKKRRK
                     VKAEAKPIHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPHGKWECPWHRCDE
                     CGSVAVSFCEFCPHSFCKAHGKGALVPSALEGRLCCSSHDPASPVSPEYWSKIRCKWE
                     SQDSGEEVKE"
     exon            750..821
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            822..984
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1139
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1655
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1782
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1883
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..1929
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2060
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2189
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2316
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2514
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2515..2685
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2832
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2833..2989
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2990..3192
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3193..3305
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3306..3575
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3576..3692
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3693..3834
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3835..3941
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3942..4143
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            4144..9652
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9087..9092
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /note="hexamer: AATAAA"
     polyA_site      9111
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /note="major polyA site"
     regulatory      9625..9630
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /note="hexamer: AATAAA"
     polyA_site      9652
                     /gene="Nsd3"
                     /gene_synonym="6720429E03; A530023P05; WHISTLE; Whsc1l1"
                     /note="major polyA site"
ORIGIN      
        1 gctcgatgag agaaagggaa agttaaggat gctggagcag aacaatggat ttctctttct
       61 ctttcatgca agggatcatg ggaaacacaa ttcagcaacc acctcaactc attgactcgg
      121 ccaacatccg ccaggaggat gcctttgata accacagtga cattgttgaa gatggtggcc
      181 cgacaccctt tgaagctact ttgcaacaag gttttcaata tccacctaca acagaagacc
      241 ttcctccgct cacaaatggc tacccaccat caatcagctt gtatgaaact caaaccaaat
      301 acccgccata taatcagtat cccaatgggt cagccaacgg ttttggtgca gttagaaact
      361 ttagccctac tgactattac cattcagaaa ttccaaacac aagaccacat gaaattctgg
      421 aaaaaccttc tcctcctcag ccacctcctc ttcctcctcc tcctcctcct cctcctcctc
      481 ctccttcggt accacaaact gtgattccaa agaagacagg ctcacccgag attaaactaa
      541 aaataaccaa aactatccag aatggcaggg aattgtttga gtcttccctt tgtggagacc
      601 tcttaaatga agtacaggca agcgagcaca caaagtctaa gcatgaaagc agaaaggaaa
      661 agaggaaaaa gagcaacaga cacgagtcat ctcgatccga agagcggagg tcacacaaga
      721 ttcccaagct agagccggag ggacagaata gaccaaatga gagggtggac actgcaccag
      781 agaagccaag agaagagcca gtgctcaaag aggccatccc ggttcagcca atactgtctt
      841 ctgttccaac aacagaaaca tccactggtg ttaagttcca ggttggtgat cttgtttggt
      901 ccaaggtggg aacctaccct tggtggcctt gtatggtttc aagtgatccc cagcttgagg
      961 tccattccaa aattaacaca agaggtgccc gggaatatca tgtccaattt tttagcaacc
     1021 agccagagag ggcatgggtt catgagaaac gggtacggga atacaaaggt catgaacagt
     1081 atgaagagtt actagccgag gcagccaagc aagccagcaa tcattctgaa aagcaaaaga
     1141 ttcggaaacc ccgacctcag agagaacgtg cccaatggga cattggcatt gctcatgcag
     1201 agaaagcatt gaaaatgact cgggaggaaa gagtagagca gtacactttc atctacattg
     1261 ataagcagcc agaagaggct tcgtcccaag caaagaagaa tgttacctct aagacggaag
     1321 tcaagaaacc ccgaagacca agatctgtgc tgaacagtca gccagaacag accaatgctg
     1381 gggaggtggc ctcctcacaa tcaagtactg accttcgaag gcagagccag aggcggcata
     1441 ctagcttgga agaggaagag ccacctcctg ttaaaatcgc ctggaaaaca gcagccgcaa
     1501 ggaagtcctt accagcctcc atcacaatgc acaaagggag cctagatttg cagaagtgta
     1561 atatgtctcc agttgtgaaa attgaacaag tgtttgctct ccagaatgca acaggagatg
     1621 ggaagtttat tgatcagttt gtttattcaa cgaagggtat tggtaacaaa acagaaataa
     1681 gtgtcagggg gcaagacagg cttataatct cttcaccaag tcagagaagt gaaaagccgg
     1741 ctcagagcgc gtcatctccg gaggcaacat ctggttctgc aggcccagta gagaagaagc
     1801 agcagagaag atccatcagg actcgatctg agtcagagaa gtccgccgag gttgtgccaa
     1861 agaagaagat caaaaaggag caggttgaga cagctcccca ggcctccctg aagaccgggt
     1921 tacagaaagg tgccagcgag atttcagatt cctgtaagcc cctaaagaaa aggagtcgcg
     1981 cctcaacgga tgtagagacg gctagttgca cgtacagaga cacatctgac tccgattcta
     2041 gaggactgag tgacgggcag gtaggctttg gcaaacaagt agatagcccg tcagctactg
     2101 ctgacgcaga tgcttctgac gcgcagtcgg tggactcgag tctgtcacgg agaggtgtcg
     2161 gcacgagtaa gaaggacact gtgtgtcagg tctgtgagaa ggctggcgac tgccttgttg
     2221 cttgtgaggg ggagtgctgc cgacactttc acgtggagtg cttgggactg actgcagtcc
     2281 ctgagggcca cttcacctgt gaggagtgtg agactggaca gcacccatgt ttttcatgta
     2341 aagtgtctgg taaggatgtg aaacgttgtt ctgttagtgt ctgtggaaag ttttatcatg
     2401 aagcctgtgt ccgcaaattc cccactgcca tctttgagtc aaaaggattc cgctgtcccc
     2461 agcactgttg ctcttcctgc tctatggaga aagacatcca caaagcaagt aaaggccgca
     2521 tgatgaggtg tctgagatgc ccggtcgcct atcacgttgg agatgcgtgt gtcgctgctg
     2581 gaagtgtatc tgtgtcctcg cacattctca tctgtagtaa ccattccaag cggagtagtc
     2641 agtctgctgc tataaacgta ggcttttgtt tcgtttgtgc cagagggctg atagttcagg
     2701 accattcaga ccccatgttc agttcctatg cctataaatc ccactaccta ctgagtgaat
     2761 caaaccgtgc tgagttgatg aaattaccta tgattccttc ttcgtcagct tccaaaaaga
     2821 gatgtgagaa aggtggaaga ttgctctgct gtgaatcgtg ccctgcttcc ttccacccag
     2881 aatgcctgag catagacatg ccagaaggct gctggaattg caatgactgt aaagctggca
     2941 agaaactgca ttacaaacag attgtttggg tcaaattggg aaattacaga tggtggcctg
     3001 cagagatttg cagtcccagg tctgtgccgc tgaacatcca gggccttaag catgacttgg
     3061 gggacttccc cgtgttcttc tttggttctc acgactacta ctgggttcac caaggcagag
     3121 tgttccctta cgttgaagga gacaaacact ttgcagaggg acagactagc attaacaaga
     3181 ccttcaaaaa agcactggaa gaagctgcaa aacgtttcca ggagctgaaa gcccagcgtg
     3241 aaagcaagga ggctttggag atggagagga cctccaggaa accccctccc tacaagcaca
     3301 tcaaggccaa caaagtcata gggaaggtgc aggtccaggt ggctgacctg tcggagatcc
     3361 cccgctgtaa ctgcaagcct ggtgatgaga acccgtgtgg cctggagtcg cagtgtctga
     3421 acaggatgtc acagtatgag tgtcacccgc aggtgtgtcc tgctggggac cgctgtcaga
     3481 accagtgctt tacaaagagg ctgtaccccg atgcagaggt catcaagacc gagcggcgag
     3541 gatggggcct cagaaccaaa agaagcatca aaaaggggga atttgtaaac gagtatgtcg
     3601 gtgaattaat tgatgaagaa gagtgcagac tccgaatcaa gcgagcccac gagaacagtg
     3661 taactaattt ttatatgtta actgttacca aggaccgtat aattgatgct ggaccaaagg
     3721 gaaattattc tcgtttcatg aaccacagtt gtaatccaaa ctgtgagaca cagaagtgga
     3781 ctgtaaatgg agacgttcga gttggactat ttgctctttg tgacattcct gcagggatgg
     3841 agttaacgtt taactataac ttggactgtc tgggcaacgg caggacagtg tgccactgcg
     3901 gagcagacaa ctgtagtgga tttctaggag tgcggccaaa gtctgcatgc acatcagcag
     3961 ttgacgagaa gacaaaaaat gccaagttga agaagaggcg gaaagtcaaa gcggaggcaa
     4021 agccaatcca tgaggattat tgttttcagt gtggggatgg tggagaactg gtcatgtgtg
     4081 acaagaaaga ctgtcccaaa gcttaccacc tcctatgcct taacctgact cagccacccc
     4141 acggaaagtg ggagtgcccg tggcatcggt gtgacgagtg tggcagtgta gccgtttcct
     4201 tctgtgaatt ctgtccacac tcattctgta aggctcatgg aaaaggggct ctggtgccct
     4261 ctgcactaga aggtcgtctc tgctgctcca gccacgaccc tgcatctcct gtgtcaccag
     4321 aatactggag caagatcaga tgtaaatggg aatcccaaga tagtggagaa gaagtaaagg
     4381 agtgagcggt ggtgacctcc tcccactgct ctcagtaaag aggcgcagag aggccacgcc
     4441 tgccctgccc tgccgtcagt ccgccttgac ggagatgggc gccagctcac acaggctaaa
     4501 gcacttgctg gtgtctgggt tttctatttt gttgtttggg ggataatttt gtggacagca
     4561 aaattccctt ggtctcttct tgcctttcat tgtgcttcag tgtctttgca atcagcaaaa
     4621 gaggtgtcct tgtttttaga gttgagagag agaaaattaa aagaaagttt tgtctatacg
     4681 atcagtgtaa agcctaagat gtactcccgg gttatgtaaa gtgaaagtag tcattcctgt
     4741 atttattttc atttttaggt attctgagaa gtgtagttca aacaatctaa cctccatgag
     4801 tgctcgtgcg tgtacgtgcg cgtgtgcgtg tgtgtgcaag cgtgcatgac tgtcaccttt
     4861 atatacctct ttcctgagcc atagagtggc taacttctct ttcctttttc ccgggccttt
     4921 tctcgggtca gagaaggctc tcagcaagta ttcagactgg cctcttgaaa acaaactcct
     4981 tgacagcatt tacctctgtg gtcttaaact gggtgggctt catggtgcgc caactctacg
     5041 tagaaatatc ttagagggac aaggagtgag tgcattctac tcattgtgat tttctgaaac
     5101 atttgaggga tgtgaataca taggggaatg ctcattcctg ctgtcctgac cactggcaag
     5161 aggctaaaga aagataaggc acagaccaaa cattggatca tatatgcacc attttctgac
     5221 caatttaatg tagactccag tctaacctgg aatttaaggg cattttattt tctgtctttc
     5281 cctctgccaa tcatgtttcc tttgccagct gggtcactca gtcatgtttc ctggtaaagc
     5341 tatataactg aatctgaatt atatgaaata gactttttca gttgatttcc taacttaggg
     5401 atgatgcaag tggacagaaa catccctaag ttatatgtga accatttcta cttagtatta
     5461 tgcaatactt aagactgtcc ccattgcata aggggaagaa cttgctcttt tacagggtat
     5521 gtaggagcca cagagatgct ccatgtagaa agcacttgaa accgcccccc acaaagtcag
     5581 tgtttgcaaa catggctgtg aggtttgaat agtacagacc caagcatacc aaggcgtcgg
     5641 cccgcagaat tcacttgcat ctagcactta ggatctagtc cagtattact tgtgtgaaat
     5701 catgtcagac atggtaacat tagactaaat aaaaatctag tgttaccatg aaatatgaac
     5761 ttcctcagtg tgtttcaact gcttactgga ggtacccagt aggctctctt ctcctcaaga
     5821 acctatactt tactaacgta gggcacgaga tgctctatga caccctgtag ttatgttagg
     5881 tgcgtttgtc ctttttttag ttttttacaa atcaattcag acctcatctc tggacaaaag
     5941 aaagagcaat tttgtgcctc ttttttttaa atatatataa atgtgcaatt tataggcaac
     6001 agacactaca gttcctctct aagctcctga actgccattt acctagtcta gcacttgtgg
     6061 taatcccgct ggctagacaa gctcctgtga ggagggagac aggacggtac ccagcaccca
     6121 gtgcagtccc tacgaccgac tcgaacgaag cacagcccac gcaaacacta aactctctgc
     6181 tcttgagcaa ttatgaagtt tgatgtcctt taaaacaata atgtaagagt atctgggttt
     6241 tgtcatatta aattttattt tatacagaca taacattttt taaacttgtt gataagcgac
     6301 ttcctttgat attccaaatg taaccctcat gagaacaatg tccgatatga gattgtatct
     6361 tgtttccttt ttgacctcgt gctttttagt atctgataac ttttatagag taagccccag
     6421 ggttcatcat gtatttataa ataccctacc ctgacctatc tctctcctgc ttctctgcta
     6481 tggctcgctt gtttttatat ctataaaagg actttgcact tttcagatct tttcctacta
     6541 gatgactaac aggcttgctc tcagaagcct gcctgctcca agccaagtgc aagggcgact
     6601 gcctgtgcca tcatcgcagc ctgggtctta gaactgaggg ttaggaagca gaacgttggc
     6661 tgactggtgt aagtatacgt acaccgtcgg tcgtttcatg tcctgtgtca gtgacattgc
     6721 aaggcactgt gcttactcgg agcctgaagc tgcttggtgg tagcttttca atatggttta
     6781 ggtatacagc atttgtggtt gattttattc agcccagaaa taaaggactc aaagcagcat
     6841 gggatactag tggtatttcc taacctcagg aggaagatga gagttctggt tatcagtgga
     6901 aagctttaga aattgggctg gaaagagcta gagatgatag cacgagacca tcacaagcac
     6961 tgtgccttgt aggtgtcgct gggccgccag ggtagctgcc tgtgtctggc caagcaggat
     7021 gggaccagtg ctcgtcactg ccagtgtgct gcagtaacct ctgatttcag tctccagtca
     7081 gggtggggat cagagtggag taggttttat cttcactttt gctttgcaag ccctacctac
     7141 tcttatttta attctcaaaa attacctttc ctaagctgta gaatagactt gagttttctg
     7201 tgcgttatat aaataacata tatatatata taaatataaa atgttttata tatataagtg
     7261 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtacacat acatactgag
     7321 ttaggaagta tttgaaacta aagagataat aagggcctgc agcccactgc ttctgtcagt
     7381 gtctcccact gcagtgtttg cggaagtgtg tgtaactaat atggatctga gatactgagg
     7441 gtctgtctgc tcatgtgaaa tactcggctg gactggtagg cagcttttat gcagtggctt
     7501 cttttttgag tactttctta ctgtttcggg tcaaagccag actacagttt gaagtcctcc
     7561 cattatctgt tcagaacata gacccggctc tccaagacct gccgctaatc ttgtcaaaac
     7621 aacactcctc ttgcagcact tccccacgga gctaaaatgc tgccaaccac atgccatgcc
     7681 ctaatgggag agggagacaa attgcctcag tctccctgag cgtgagcagc gtatggtctt
     7741 tatgtttctc aaggcactgc ttcatttcta gacttgaatt gcttttcacg tggctgttgc
     7801 atctgaccat cccggactct gccaggctgc taagggcagc gtggaagtga tcggatggca
     7861 aggtcctcag gaaggaccgc ccacacttgg tctttgctta tttgtaacag aatggttggt
     7921 tacggaaagg tctggtccct ccttggatgg acgaagggat ccgtgtaggc agctttgcag
     7981 tggattgttg tatggactga ggtgtcacag ttttcacctt gggtggggga atgggaatgt
     8041 ggggagaaag ggagggaaag gtacagtgca gttcccctaa caccgagacc cccccacccc
     8101 atttgaattc aggctcttca attccttggg acttcagatt ctcattttat attttacagt
     8161 tttaaaatta agacatggaa agcatcatgt tgtatactgt cagatatgca gtcctcccga
     8221 cgggagcacg ggcatcttag gtttcttggc acctttgagg tcggagctcg tgcatgttga
     8281 gaaatgattt ccctgcgtct ttgttttgcc tttttctcct ttccttgcct cacccctggg
     8341 tagctggtct ttctgtttaa catatgcttg tgtgggggga agagggcgca cttatgttat
     8401 actcctaaaa tgctatgtat aacctttgtt tcaaaaaccc atttccactg ggcatggttg
     8461 cacacgcctt taatcctagc actccagaag cagaggcagg aggatctccg agagttcaag
     8521 gcctacctaa cgcttcccag ccagccagga ctatgtagag agaccctgtc tcaaaaaggg
     8581 ggggaaaatc aattgtttgg cttttgtcaa tccattgcaa aagtggcaga tgtgagcgct
     8641 tgtatttgtg tatgggggag gggggagaaa agtattgttt gtccttcagt aggctctgaa
     8701 agtcattgac ttgtaagttc aagggaaaat agaagttgcc aaagtcagta ttcggtcctt
     8761 ggttttcttg tgacttcact gcgtccgtta agggacgaga agccttcctt acctgctgac
     8821 tgagccttgc tctgtgcaga tggctttcaa gtatctgcca ttttgcctca acagggaatt
     8881 cttgtcagtt aaagggaatg agggaattac acttttgtta tcaattaaca tctcattttt
     8941 tgtaccctat ggtaagtaac tgtattgccg ctaataacca agttgctaac tgattacagc
     9001 tggctaaaaa gttcttttta tatacaaaga tttgtgtatt ttcaactgct atggcaaatt
     9061 catttgtgtc catttaaata ttacacaata aacacatatc tgcagatccc accctcacct
     9121 tgtgttcttt gaataaagtt cttgatagat tgtttacatc tgtatttacc tgtaatgaac
     9181 gctgtagacc tgtgaaatat caaagtgtca acatccatta gtcagtctct ttagtttctg
     9241 tgacttggcg ctcctaacac taaggggtta gtgttaatgt tccccagggg tattctacta
     9301 gggcattact gtttaatgac ttcaaaatat agtttaagga ttttgtttat tgaccaatgt
     9361 cttattgcaa agtatgagcc taccatctta gttaacctgt acatatctgt taactagtgt
     9421 agtgtatgtg agattgcctg taacatttga gcttcttact gatgtgtgct ggtgggacag
     9481 gttttgtaca ttgtatttac tggaatcctg cctttttaac ttacagatac tttagaattc
     9541 aaatatgttt tctgcttctg taagaatgtg ggaaagcttt taatggcatt ccattgattt
     9601 caggtcttgc ttgaggttga ctttaataaa ttgtcctgta tggtctactt aa
//
