LOCUS       NM_175231               6933 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus nuclear receptor binding SET domain protein 2 (Nsd2),
            transcript variant 2, mRNA.
ACCESSION   NM_175231
VERSION     NM_175231.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Shipman,G.A., Padilla,R., Horth,C., Hu,B., Bareke,E.,
            Vitorino,F.N., Gongora,J.M., Garcia,B.A., Lu,C. and Majewski,J.
  TITLE     Systematic perturbations of SETD2, NSD1, NSD2, NSD3, and ASH1L
            reveal their distinct contributions to H3K36 methylation
  JOURNAL   Genome Biol 25 (1), 263 (2024)
   PUBMED   39390582
  REMARK    GeneRIF: Systematic perturbations of SETD2, NSD1, NSD2, NSD3, and
            ASH1L reveal their distinct contributions to H3K36 methylation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Goto,N., Suke,K., Yonezawa,N., Nishihara,H., Handa,T., Sato,Y.,
            Kujirai,T., Kurumizaka,H., Yamagata,K. and Kimura,H.
  TITLE     ISWI chromatin remodeling complexes recruit NSD2 and H3K36me2 in
            pericentromeric heterochromatin
  JOURNAL   J Cell Biol 223 (8) (2024)
   PUBMED   38709169
  REMARK    GeneRIF: ISWI chromatin remodeling complexes recruit NSD2 and
            H3K36me2 in pericentromeric heterochromatin.
REFERENCE   3  (bases 1 to 6933)
  AUTHORS   Feng,W., Niu,N., Lu,P., Chen,Z., Rao,H., Zhang,W., Ma,C., Liu,C.,
            Xu,Y., Gao,W.Q., Xue,J. and Li,L.
  TITLE     Multilevel Regulation of NF-kappaB Signaling by NSD2 Suppresses
            Kras-Driven Pancreatic Tumorigenesis
  JOURNAL   Adv Sci (Weinh) 11 (30), e2309387 (2024)
   PUBMED   38889281
  REMARK    GeneRIF: Multilevel Regulation of NF-kappaB Signaling by NSD2
            Suppresses Kras-Driven Pancreatic Tumorigenesis.
REFERENCE   4  (bases 1 to 6933)
  AUTHORS   Kawai,T., Kinoshita,S., Takayama,Y., Ohnishi,E., Kamura,H.,
            Kojima,K., Kikuchi,H., Terao,M., Sugawara,T., Migita,O., Kagami,M.,
            Isojima,T., Yamaguchi,Y., Wakui,K., Ohashi,H., Shimizu,K.,
            Mizuno,S., Okamoto,N., Fukushima,Y., Takada,F., Kosaki,K.,
            Takada,S., Akutsu,H., Ura,K., Nakabayashi,K. and Hata,K.
  TITLE     Loss of function in NSD2 causes DNA methylation signature similar
            to that in Wolf-Hirschhorn syndrome
  JOURNAL   Genet Med Open 2, 101838 (2024)
   PUBMED   39669601
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6933)
  AUTHORS   Su,D., Eliason,S., Sun,Z., Shao,F. and Amendt,B.A.
  TITLE     Wolf-Hirschhorn syndrome candidate 1 (Whsc1) methyltransferase
            signals via a Pitx2-miR-23/24 axis to effect tooth development
  JOURNAL   J Biol Chem 299 (11), 105324 (2023)
   PUBMED   37806494
  REMARK    GeneRIF: Wolf-Hirschhorn syndrome candidate 1 (Whsc1)
            methyltransferase signals via a Pitx2-miR-23/24 axis to effect
            tooth development.
REFERENCE   6  (bases 1 to 6933)
  AUTHORS   Catela,C., Bilbao-Cortes,D., Slonimsky,E., Kratsios,P.,
            Rosenthal,N. and Te Welscher,P.
  TITLE     Multiple congenital malformations of Wolf-Hirschhorn syndrome are
            recapitulated in Fgfrl1 null mice
  JOURNAL   Dis Model Mech 2 (5-6), 283-294 (2009)
   PUBMED   19383940
REFERENCE   7  (bases 1 to 6933)
  AUTHORS   Powles,N., Babbs,C., Ficker,M., Schimmang,T. and Maconochie,M.
  TITLE     Identification and analysis of genes from the mouse otic vesicle
            and their association with developmental subprocesses through in
            situ hybridization
  JOURNAL   Dev Biol 268 (1), 24-38 (2004)
   PUBMED   15031102
REFERENCE   8  (bases 1 to 6933)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   9  (bases 1 to 6933)
  AUTHORS   Naf,D., Wilson,L.A., Bergstrom,R.A., Smith,R.S., Goodwin,N.C.,
            Verkerk,A., van Ommen,G.J., Ackerman,S.L., Frankel,W.N. and
            Schimenti,J.C.
  TITLE     Mouse models for the Wolf-Hirschhorn deletion syndrome
  JOURNAL   Hum Mol Genet 10 (2), 91-98 (2001)
   PUBMED   11152656
REFERENCE   10 (bases 1 to 6933)
  AUTHORS   Stec,I., Wright,T.J., van Ommen,G.J., de Boer,P.A., van
            Haeringen,A., Moorman,A.F., Altherr,M.R. and den Dunnen,J.T.
  TITLE     WHSC1, a 90 kb SET domain-containing gene, expressed in early
            development and homologous to a Drosophila dysmorphy gene maps in
            the Wolf-Hirschhorn syndrome critical region and is fused to IgH in
            t(4;14) multiple myeloma
  JOURNAL   Hum Mol Genet 7 (7), 1071-1082 (1998)
   PUBMED   9618163
  REMARK    Erratum:[Hum Mol Genet 1998 Sep;7(9):1527]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF742194.1, CF532498.1, AC163329.6 and BE986660.1.
            
            On Apr 30, 2010 this sequence version replaced NM_175231.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the central coding region, compared to variant 1,
            resulting in an isoform (2) that is 1 aa shorter than isoform 1.
            The 5' UTR of this variant may be incomplete due to the lack of
            5'-complete transcripts supporting it, and the presence of
            alternative splicing choices further upstream.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849380,
                              SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-463               CF742194.1         50-512
            464-1100            CF532498.1         1-637
            1101-1439           AC163329.6         151143-151481       c
            1440-1584           AC163329.6         145701-145845       c
            1585-1703           AC163329.6         142101-142219       c
            1704-1785           AC163329.6         139221-139302       c
            1786-1910           AC163329.6         138001-138125       c
            1911-2042           AC163329.6         127690-127821       c
            2043-2166           AC163329.6         126704-126827       c
            2167-2367           AC163329.6         125692-125892       c
            2368-2547           AC163329.6         124712-124891       c
            2548-2704           AC163329.6         124196-124352       c
            2705-2910           AC163329.6         123875-124080       c
            2911-3014           AC163329.6         121789-121892       c
            3015-3284           AC163329.6         121228-121497       c
            3285-3401           AC163329.6         119511-119627       c
            3402-3543           AC163329.6         115232-115373       c
            3544-3650           AC163329.6         114833-114939       c
            3651-3855           AC163329.6         113270-113474       c
            3856-6355           AC163329.6         109474-111973       c
            6356-6933           BE986660.1         1-578               c
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 17.83 cM"
     gene            1..6933
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /note="nuclear receptor binding SET domain protein 2"
                     /db_xref="GeneID:107823"
                     /db_xref="MGI:MGI:1276574"
     exon            1..626
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..4127
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /EC_number="2.1.1.357"
                     /note="isoform 2 is encoded by transcript variant 2;
                     probable histone-lysine N-methyltransferase NSD2; IL5
                     promoter REII region-binding protein;
                     trithorax/ash1-related protein 5; nuclear SET
                     domain-containing protein 2; wolf-Hirschhorn syndrome
                     candidate 1 protein homolog; multiple myeloma SET
                     domain-containing protein"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase NSD2 isoform
                     2"
                     /protein_id="NP_780440.2"
                     /db_xref="CCDS:CCDS51468.1"
                     /db_xref="GeneID:107823"
                     /db_xref="MGI:MGI:1276574"
                     /translation="MEFSIRKSPLSVQKVVKCMKMKQTPEILGSANGKTQNCEVNHEC
                     SVFLSKAQLSNSLQEGVMQKFNGHDALPFLPAEKLKDLTSCVFNGEPGAHDTKLCFEA
                     QEVKGIGTPPNTTPIKNGSPEIKLKITKTYMNGKPLFESSICGDGAADVSQSEENEQK
                     SDNKTRRNRKRSIKYDSLLEQGLVEAALVSKISSPADKKIPVKKESCPNTGRDRDLLL
                     KYNVGDLVWSKVSGYPWWPCMVSADPLLHNHTKLKGQKKSARQYHVQFFGDAPERAWI
                     FEKSLVAFEGEEQFEKLCQESAKQAPTKAEKIKLLKPISGRLRAQWEMGIVQAEEAAS
                     MSIEERKAKFTFLYVGDQLHLNPQVAKEAGIVTEPLGEMVDSSGASEEAAVDPGSVRE
                     EDIPTKRRRRTKRSSSAENQEGDPGTDKSTPPKMAEAEPKRGVGSPAGRKKSTGSAPR
                     SRKGDSAAQFLVFCQKHRDEVVAEHPDASGEEIEELLGSQWSMLNEKQKARYNTKFSL
                     MISAQSEEDSGNGNGKKRSHTKRADDPAEDVDVEDAPRKRLRADKHSLRKRETITDKT
                     ARTSSYKAIEAASSLKSQAATKNLSDACKPLKKRNRASATASSALGFNKSSSPSASLT
                     EHEVSDSPGDEPSESPYESADETQTEASVSSKKSERGMAAKKEYVCQLCEKTGSLLLC
                     EGPCCGAFHLACLGLSRRPEGRFTCTECASGIHSCFVCKESKMEVKRCVVNQCGKFYH
                     EACVKKYPLTVFESRGFRCPLHSCMSCHASNPSNPRPSKGKMMRCVRCPVAYHGGDAC
                     LAAGCSVIASNSIICTGHFTARKGKRHHTHVNVSWCFVCSKGGSLLCCEACPAAFHPD
                     CLNIEMPDGSWFCNDCRAGKKLHFQDIIWVKLGNYRWWPAEVCHPKNVPPNIQKMKHE
                     IGEFPVFFFGSKDYYWTHQARVFPYMEGDRGSRYQGVRGIGRVFKNALQEAEARFNEV
                     KLQREARETQESERKPPPYKHIKVNKPYGKVQIYTADISEIPKCNCKPTDENPCGSDS
                     ECLNRMLMFECHPQVCPAGEYCQNQCFTKRQYPETKIIKTDGKGWGLVAKRDIRKGEF
                     VNEYVGELIDEEECMARIKYAHENDITHFYMLTIDKDRIIDAGPKGNYSRFMNHSCQP
                     NCETLKWTVNGDTRVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSGFLGD
                     RPKTSASLSSEEKGKKAKKKTRRRRAKGEGKRQSEDECFRCGDGGQLVLCDRKFCTKA
                     YHLSCLGLGKRPFGKWECPWHHCDVCGKPSTSFCHLCPNSFCKEHQDGTAFRSTQDGQ
                     SYCCEHDLRADSSSSTKTEKPFPESLKSKGKRKKRRCWRRVTDGK"
     exon            627..789
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            790..956
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1439
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1584
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1703
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1785
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1910
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2042
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2166
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2367
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2547
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..2704
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2705..2910
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2911..3014
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3284
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            3285..3401
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            3402..3543
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            3544..3650
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            3651..3855
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     exon            3856..6916
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4422..4427
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /note="hexamer: ATTAAA"
     polyA_site      4445
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /note="major polyA site"
     regulatory      6665..6670
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /note="hexamer: ATTAAA"
     regulatory      6669..6674
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /note="hexamer: AATAAG"
     polyA_site      6684
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
     polyA_site      6688
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
     regulatory      6897..6902
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /note="hexamer: ATTAAA"
     polyA_site      6916
                     /gene="Nsd2"
                     /gene_synonym="5830445G22Rik; 9430010A17Rik;
                     C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090;
                     MMSET; Whsc1; Whsc1l"
                     /note="major polyA site"
ORIGIN      
        1 tgttctaaga accaaaggat ctaggctgga tggaatttag catcagaaaa agtcctcttt
       61 ctgttcagaa agttgtaaag tgcatgaaga tgaagcagac accagaaatc cttggcagtg
      121 caaatggaaa gactcagaac tgtgaagtga atcatgaatg ttctgtattc ctcagcaaag
      181 ctcaactttc taacagccta caggaggggg tcatgcagaa atttaatggc catgatgcgc
      241 tcccctttct tccagcagag aagttgaaag atcttacttc ttgtgttttt aatggagaac
      301 ctggtgctca tgatactaaa ttgtgttttg aggcccagga agtaaaagga attgggacac
      361 cacccaatac tacacctatc aaaaatggct ctccagaaat taaactgaaa atcaccaaaa
      421 catacatgaa tgggaaacct ctctttgaat cttcaatttg tggtgacggt gctgctgatg
      481 tgtctcagtc agaagaaaac gaacaaaaat ctgataataa gactaggagg aacaggaaga
      541 ggagcataaa atatgactct ttactggagc agggccttgt ggaagcagct ttagtgtcca
      601 agatctcaag tcctgcagat aaaaagattc cagttaagaa ggagtcctgt ccaaatactg
      661 gcagagacag agacctcttg ttaaaataca acgttggtga tttggtgtgg tccaaagtgt
      721 caggttaccc ttggtggcct tgcatggttt ctgctgaccc actccttcac aatcatacca
      781 aacttaaagg tcagaaaaaa agtgcacgcc agtatcatgt acagttcttt ggtgatgccc
      841 cagaaagagc ttggatattt gagaagagcc ttgttgcttt tgaaggagaa gaacaatttg
      901 aaaaattatg ccaagaaagt gccaagcagg cacccacgaa agctgagaaa atcaagttgt
      961 tgaaacctat ttcggggaga ttgagagccc agtgggaaat gggcattgtc caagctgagg
     1021 aagctgccag catgtcaata gaggagcgga aagccaaatt taccttcctt tatgtggggg
     1081 accaattgca cctcaaccct caagtagcta aggaggctgg cattgttaca gaacctttgg
     1141 gagaaatggt ggactcttca ggggccagtg aagaggctgc tgtagaccct gggtctgtga
     1201 gagaagagga tattcccacg aagagaaggc gaagaaccaa aaggtctagt tctgctgaga
     1261 accaagaagg tgatcctggc acagacaaga gtacacctcc aaagatggca gaggctgaac
     1321 ctaagcgagg agtaggctct cctgctggga ggaaaaagtc cacaggctct gctcctcgga
     1381 gcaggaaggg agactcagca gcccagtttt tagtcttttg tcaaaaacac agagatgagg
     1441 ttgtagctga acatccagat gcctcagggg aagagattga agaattgctt gggtcccagt
     1501 ggagcatgct caatgaaaag cagaaagcac gatataatac aaagttttcc ctaatgatct
     1561 ctgcccagtc tgaagaagac tctggaaatg ggaatgggaa aaaaagaagc cacacaaaga
     1621 gagcagatga ccctgcagag gatgtggatg ttgaagacgc gcccaggaaa agacttagag
     1681 cagataagca cagtcttcgg aagagagaga caatcaccga caaaacggcc agaacaagct
     1741 cttacaaggc tatagaggca gcctcctcac tcaagagcca ggcagcaacg aaaaatttgt
     1801 ctgatgcttg caaaccactg aagaagcgaa atcgggcttc tgcaacagcg tcttcagcac
     1861 ttgggtttaa caaaagttca tctccttctg catccttaac tgagcatgag gtctcagaca
     1921 gcccaggaga tgaaccatca gagtctccat atgaaagtgc agatgaaaca cagactgaag
     1981 catccgtctc atctaaaaaa tctgaaagag gaatggctgc caaaaaggag tacgtgtgtc
     2041 agctgtgtga gaagacaggc agtctcttac tgtgtgaggg cccctgttgt ggagcattcc
     2101 acctagcctg ccttggactt tcccgaagac cggaaggaag attcacctgc accgaatgtg
     2161 cctcagggat tcactcatgc ttcgtatgta aagaaagcaa gatggaggtg aaacgttgtg
     2221 tggtaaatca gtgtggaaag ttttaccatg aagcctgtgt aaaaaaatac cctctcactg
     2281 tgtttgagag ccgaggcttc cgctgtcctc tccatagctg tatgagctgc catgcctcca
     2341 acccttcaaa tccacggcca tcaaaaggta aaatgatgcg atgtgtccga tgccccgttg
     2401 cctatcatgg aggggatgcc tgtctggcag caggatgttc ggtgattgct tctaacagca
     2461 taatctgcac aggccacttc actgcccgga aggggaagcg gcaccatacc catgtcaatg
     2521 tgagctggtg ctttgtgtgc tccaaagggg gaagccttct gtgctgtgag gcctgcccag
     2581 cagcctttca ccccgattgt ctaaacatcg agatgcccga tggtagctgg ttctgcaacg
     2641 attgccgggc tggaaagaag ctccactttc aagacatcat ctgggtcaaa cttggtaact
     2701 acagatggtg gccggcagaa gtttgccatc ccaaaaatgt tcccccaaat attcagaaaa
     2761 tgaagcacga gattggagaa ttccctgtat ttttctttgg gtctaaagat tattactgga
     2821 cgcatcaggc acgagtgttc ccatacatgg agggggaccg gggcagccgc taccaggggg
     2881 tcagagggat cggaagagtc ttcaagaacg cattgcaaga agctgaagct cgttttaatg
     2941 aagtcaaact tcagcgggaa gcccgagaaa cacaggagag tgagagaaag cctccaccat
     3001 acaagcatat caaggtgaat aaaccttatg gtaaagtcca gatctacaca gctgacattt
     3061 ctgaaatccc gaagtgcaac tgtaagccca cagatgagaa cccctgtggc tctgattcag
     3121 agtgtctgaa caggatgcta atgtttgagt gccacccgca ggtgtgtcct gcaggggaat
     3181 actgccagaa ccagtgtttc actaagcgcc agtaccctga gaccaagatc atcaagacag
     3241 atggcaaagg gtggggcctg gttgccaaaa gggacatcag aaaaggagaa tttgttaatg
     3301 agtatgttgg tgagctgatt gatgaagagg agtgtatggc aagaatcaaa tatgcacatg
     3361 agaatgacat cactcacttc tacatgctca ccatagacaa ggaccgcata attgatgctg
     3421 gccccaaagg gaattattca cgattcatga accatagctg ccagcccaat tgtgagaccc
     3481 tgaagtggac agtgaatggg gacacacggg ttggcctgtt tgctgtgtgt gacattcctg
     3541 caggtacaga gctgactttc aactataacc ttgattgttt gggcaatgaa aagacagtct
     3601 gtcggtgtgg agcctccaac tgtagtgggt tccttggaga cagaccaaag acatcagcat
     3661 ccctttcatc agaggaaaag ggtaaaaagg ccaagaagaa aaccagaagg cgcagagcca
     3721 agggtgaagg aaaacggcag tcagaggatg agtgcttccg ctgtggtgat ggtgggcagc
     3781 tggtgctgtg tgaccgcaag ttctgtacca aggcctacca cctatcctgc ctcggtttgg
     3841 gcaagcggcc ctttgggaag tgggaatgtc cttggcatca ctgtgatgta tgtggcaaac
     3901 cttctacctc attttgccac ctctgcccca actcattctg taaggaacac caagatggga
     3961 ctgctttccg ttccacccag gatgggcagt cctactgctg tgagcatgac ttgagggcag
     4021 actcttcaag tagcaccaag actgagaaac cctttccaga atcactgaag tcaaaaggaa
     4081 agaggaagaa aaggcggtgc tggcgaaggg tcacagatgg caaatagcgc ttggtcagat
     4141 cctggggtgg tgtggggcag ccagccctgc agacagtggg aaggcaagca cggcacccac
     4201 cccaaggacc cagctcctag cgctgtgtat aggcctgctt gggtgggagg cagctcctcc
     4261 caccactgag ccatcctcag cagctgcgct gcgtctgcac tgatgcctgt cagctgcagg
     4321 caggcttggt gggccaggat aaacaagcct tactacacag cgccacctct acacttgaat
     4381 ctgagaccca gcgtcaaggt gccctcccat ttctatttta gattaaagtt atgattgaag
     4441 attaactggc atgtgaaatg cttttatctt acctgaaaca aagaattttc taaaggtcac
     4501 cattagctct gtcctgatgg tgcaggcttc tattcacata ctgcccacct actcagggca
     4561 gactgggcct ccagccctgg gctccttccg atcaggattg gtaattcttc tgtttgaaat
     4621 agcctatgtt ctttgtatta atgtgaattt ttcctcaaag tgctttgttt ccactttgag
     4681 tggttgtggt gttgtaagat tcctctcgtg gcttccctgt ttgtggacat gaccagtgac
     4741 agcagtgtgg cctgtggagc agagcagctg cctttgtttt gcctgttttt ctctaatgtc
     4801 ttctgaaaac cttgtacatt ttcgtcactt ggtatgtttc tgacatttat tcccaacata
     4861 cttttgctaa aacacattac cagctaaact gtatctgagt tgtcgggcag atataaacta
     4921 ttgagcacag agggaggtaa ccacagtatc tggggtgggg gtgggggggt ggcacaaggc
     4981 agcctgaaac ctaggctaat gctttctgtt tggtgcattt gtttttggag cctaaagctg
     5041 gttgcccgtt ctctctccac ctggtttcat ggtggtcaag actgatggta tctgtttccc
     5101 tgtgagattt aagtgattta ttttaaaggt ccagctcaag gactactaga ctgtctgtcc
     5161 tttggcttgc ttctgccttc ccaggccttg gagagcttgg gacaccaaga aaggaaacct
     5221 catgctttct ttctattggg agggaaggtg cacacaatgc atcaacaatg gatggctttg
     5281 gaaaaaccac aaagttaaac tagccattaa gtccccaagg tttgctactc actgctgtcc
     5341 tgctgctctc tagccttgta gaactgcagt ctccatttga accaggactg agtactctta
     5401 gtggtcagca tcagtcatga cacccgaaag ccgagcatgt cgtgagcact gatcagagac
     5461 cactcctttg gcacttctat agtagtcccc caaaagttga agcagctttg caggcagcct
     5521 gttgcttcca taggcacagg cctagttacc tctggagacg cctatttgac tggacaaggt
     5581 caggaacact tttcagttta gaatttaatc tggctcaaat tgctctataa aaatactaga
     5641 ctagttcata aagttctgtt acttatggtc ccaatttgaa aggctgatgt agtgataggc
     5701 cacatagcct cctcacatgc acatggcccc caggctccat ggtgggccca tcttgtccca
     5761 aaccatcctt tcctaccagc tgagtagctt ctgcggaaga gaacagcagc agagctgatt
     5821 ctgcagtaca ctttgggaac aaattaactt taaaaaaaaa acttaagtaa aatcttggga
     5881 tttttggttc caaattattt tatagtcttt ctgaaggaaa tgattgtgga catgttaaga
     5941 cagtttcccc tttctaaaag tattaatgaa taataacact aactctaaaa accaaccgaa
     6001 gacctattta caaatctggg gtgattggcc tttccaacag gcctagggct gtgactctgg
     6061 gtgtgatagg tgtcctgaat ctgacagaga tgtttctttc tgaatatcaa ctccagtttc
     6121 ttcttcctta atgtgacata aacaaatgaa aactgcccat cttccatact actcaggagc
     6181 cttggcatcc caaaccaggc aggagctgct ccctagtcag cacacacccg tgtgtatgct
     6241 tctgcagagt ggcagcagcc caggatccac agccagtgtc cacagtagca ctggcctagg
     6301 agaatgtgga accacagcac ttcagaccta ggcacctgta gggcagtgca tctggcacga
     6361 gtgaggagga cccaggtgcc agttcctaag gaacagcctg gcactagtat gtaaggactc
     6421 cttgggtctc aggcaggcac actcaggttg gcttttctga ggacttccaa gtgatacctg
     6481 atgatgtgtg acctccagag ctacctaatt ttcaagagtt aaaggaaaaa aatgttttaa
     6541 agttaatgca aacacctgtc agttgccaaa taacttcagc tgataactgt tagaataaaa
     6601 tgctgtgtcc atgtgtatca tggtgtcact gtatcctgtt gagatgtgaa atgccctatc
     6661 agaaattaaa taagtttaaa tgtgccaatt ggtgtctaaa cttcatacaa tataaagtca
     6721 ggttcctttt aggaatgctg ggtgctgtca ctaggtctga tagtgaaggc ttaaaaactt
     6781 gaagtttggg gggagggggg agttaatact gaaatataga tgagaattac cagttgggga
     6841 agaatagcat ttaaagtgga aaatcgtgtt tggaaaactg taatgagtat ttttttatta
     6901 aaaaacttta aaggccaaaa aaaaaaaaaa aaa
//
