LOCUS       NM_008739              12784 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus nuclear receptor-binding SET-domain protein 1 (Nsd1),
            transcript variant 1, mRNA.
ACCESSION   NM_008739 XM_622088
VERSION     NM_008739.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Shipman,G.A., Padilla,R., Horth,C., Hu,B., Bareke,E.,
            Vitorino,F.N., Gongora,J.M., Garcia,B.A., Lu,C. and Majewski,J.
  TITLE     Systematic perturbations of SETD2, NSD1, NSD2, NSD3, and ASH1L
            reveal their distinct contributions to H3K36 methylation
  JOURNAL   Genome Biol 25 (1), 263 (2024)
   PUBMED   39390582
  REMARK    GeneRIF: Systematic perturbations of SETD2, NSD1, NSD2, NSD3, and
            ASH1L reveal their distinct contributions to H3K36 methylation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  AUTHORS   Shao,R., Suo,J., Zhang,Z., Kong,M., Ma,Y., Wen,Y., Liu,M.,
            Zhuang,L., Ge,K., Bi,Q., Zhang,C. and Zou,W.
  TITLE     H3K36 methyltransferase NSD1 protects against osteoarthritis
            through regulating chondrocyte differentiation and cartilage
            homeostasis
  JOURNAL   Cell Death Differ 31 (1), 106-118 (2024)
   PUBMED   38012390
  REMARK    GeneRIF: H3K36 methyltransferase NSD1 protects against
            osteoarthritis through regulating chondrocyte differentiation and
            cartilage homeostasis.
REFERENCE   3  (bases 1 to 12784)
  AUTHORS   Zheng,Y., Zhao,C., Song,Q., Xu,L., Zhang,B., Hu,G., Kong,X., Li,S.,
            Li,X., Shen,Y., Zhuang,L., Wu,M., Liu,Y. and Zhou,Y.
  TITLE     Histone methylation mediated by NSD1 is required for the
            establishment and maintenance of neuronal identities
  JOURNAL   Cell Rep 42 (12), 113496 (2023)
   PUBMED   37995181
  REMARK    GeneRIF: Histone methylation mediated by NSD1 is required for the
            establishment and maintenance of neuronal identities.
REFERENCE   4  (bases 1 to 12784)
  AUTHORS   Chen,C., Shin,J.H., Fang,Z., Brennan,K., Horowitz,N.B., Pfaff,K.L.,
            Welsh,E.L., Rodig,S.J., Gevaert,O., Gozani,O., Uppaluri,R. and
            Sunwoo,J.B.
  TITLE     Targeting KDM2A Enhances T-cell Infiltration in NSD1-Deficient Head
            and Neck Squamous Cell Carcinoma
  JOURNAL   Cancer Res 83 (16), 2645-2655 (2023)
   PUBMED   37311054
  REMARK    GeneRIF: Targeting KDM2A Enhances T-cell Infiltration in
            NSD1-Deficient Head and Neck Squamous Cell Carcinoma.
REFERENCE   5  (bases 1 to 12784)
  AUTHORS   Hamagami,N., Wu,D.Y., Clemens,A.W., Nettles,S.A., Li,A. and
            Gabel,H.W.
  TITLE     NSD1 deposits histone H3 lysine 36 dimethylation to pattern non-CG
            DNA methylation in neurons
  JOURNAL   Mol Cell 83 (9), 1412-1428 (2023)
   PUBMED   37098340
  REMARK    GeneRIF: NSD1 deposits histone H3 lysine 36 dimethylation to
            pattern non-CG DNA methylation in neurons.
REFERENCE   6  (bases 1 to 12784)
  AUTHORS   Rayasam,G.V., Wendling,O., Angrand,P.O., Mark,M., Niederreither,K.,
            Song,L., Lerouge,T., Hager,G.L., Chambon,P. and Losson,R.
  TITLE     NSD1 is essential for early post-implantation development and has a
            catalytically active SET domain
  JOURNAL   EMBO J 22 (12), 3153-3163 (2003)
   PUBMED   12805229
  REMARK    GeneRIF: Data show that nuclear receptor-binding SET
            domain-containing protein (NSD1) is a developmental regulatory
            protein with histone methyltransferase activity that exerts
            function(s) essential for early post-implantation development.
REFERENCE   7  (bases 1 to 12784)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 12784)
  AUTHORS   Kurotaki,N., Harada,N., Yoshiura,K., Sugano,S., Niikawa,N. and
            Matsumoto,N.
  TITLE     Molecular characterization of NSD1, a human homologue of the mouse
            Nsd1 gene
  JOURNAL   Gene 279 (2), 197-204 (2001)
   PUBMED   11733144
REFERENCE   9  (bases 1 to 12784)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 12784)
  AUTHORS   Huang,N., vom Baur,E., Garnier,J.M., Lerouge,T., Vonesch,J.L.,
            Lutz,Y., Chambon,P. and Losson,R.
  TITLE     Two distinct nuclear receptor interaction domains in NSD1, a novel
            SET protein that exhibits characteristics of both corepressors and
            coactivators
  JOURNAL   EMBO J 17 (12), 3398-3412 (1998)
   PUBMED   9628876
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK145783.1, AC160958.2, CD549506.1, BQ442794.1, CB247038.1,
            CD806124.1, CN529078.1, CA318563.1, BQ917954.1 and BI737132.1.
            
            On Dec 7, 2006 this sequence version replaced NM_008739.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1571              AK145783.1         2-1572
            1572-3910           AC160958.2         109681-112019       c
            3911-4367           CD549506.1         93-549
            4368-4487           BQ442794.1         39-158
            4488-5199           CB247038.1         39-750
            5200-5565           CD806124.1         228-593
            5566-5762           CN529078.1         302-498
            5763-6310           CA318563.1         186-733
            6311-6484           BQ917954.1         20-193
            6485-6705           BI737132.1         126-346
            6706-12784          AC160958.2         39433-45511         c
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 29.8 cM"
     gene            1..12784
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /note="nuclear receptor-binding SET-domain protein 1"
                     /db_xref="GeneID:18193"
                     /db_xref="MGI:MGI:1276545"
     exon            1..94
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            95..1041
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..8187
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /EC_number="2.1.1.357"
                     /note="isoform 1 is encoded by transcript variant 1;
                     histone-lysine N-methyltransferase, H3 lysine-36 and H4
                     lysine-20 specific; H3-K36-HMTase; H4-K20-HMTase;
                     NR-binding SET domain-containing protein; nuclear
                     receptor-binding SET domain-containing protein 1;
                     histone-lysine N-methyltransferase, H3 lysine-36 specific"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase, H3 lysine-36
                     specific isoform 1"
                     /protein_id="NP_032765.3"
                     /db_xref="CCDS:CCDS36673.1"
                     /db_xref="GeneID:18193"
                     /db_xref="MGI:MGI:1276545"
                     /translation="MDRTCELSRRNCLLSFSNPVNLDASEDKDSPFGNGQSNFSEPLN
                     GCTMQLPTAASGTSQNAYGQDSPSCYIPLRRLQDLASMINVEYLSGSADGSESFQDPA
                     KSDSRAQSPIVCTSLSPGGPTALAMKQEPTCNNSPELQLRVTKTTKNGFLHFENFTGV
                     DDADVDSEMDPEQPVTEDESIEEIFEETQTNATCNYEPKSENGVEVAMGSEQDSMPES
                     RHGAVERPFLPLAPQTEKQKNKQRSEVDGSNEKTALLPAPTSLGDTNVTVEEQFNSIN
                     LSFQDDPDSSPSPLGNMLEIPGTSSPSTSQELPFCQPKKKSTPLKYEVGDLIWAKFKR
                     RPWWPCRICSDPLINTHSKMKVANRRPYREYYVEAFGDPSEKAWVAGKAIVMFEGRHQ
                     FEELPVLRKRGKQKEKGYRHKVPQKILSKWEASVGLAEQYDVPKGSKNQKCVSSSVKL
                     DSEEDMPFEDCTNDPDSEHLLLNGCLKSLAFDSEHSADEKEKPCAKSRVRKSSDNIKR
                     TSVKKDLVPFESRKEERRGKIPDNLGLDFISGGVSDKQASNELSRIANSLTGSSTAPG
                     SFLFSSSVQNTAKTDFETPDCDSLSGLSESALISKHSGEKKKLQPGQVCSSKVQLCYV
                     GAGDEEKRSNSVSVSTTSDDGCSDLDPTEHNSGFQNSVLGITDAFDKTENALSVHKNE
                     TQYSRYPVTNRIKEKQKSLITNSHADHLMGSTKTMEPETAELSQVNLSDLKISSPIPK
                     PQPEFRNDGLTTKFSAPPGIRNENPLTKGGLANQTLLPLKCRQPKFRSIKCKHKESPA
                     VAETSATSEDLSLKCCSSDTNGSPLANISKSGKGEGLKLLNNMHEKTRDSSDIETAVV
                     KHVLSELKELSYRSLSEDVSDSGTAKASKPLLFSSASSQNHIPIEPDYKFSTLLMMLK
                     DMHDSKTKEQRLMTAQNLASYRTPDRGDCSSGSPVGTSKVLVLGSSTPNSEKPGDSTQ
                     DSVHQSPGGGDSALSGELSSSLSSLASDKRELPACGKIRSNCIPRRNCGRAKPSSKLR
                     ETISAQMVKPSVNPKALKTERKRKFSRLPAVTLAANRLGNKESGSVNGPSRGGAEDPG
                     KEEPLQQMDLLRNEDTHFSDVHFDSKAKQSDPDKNLEKEPSFENRKGPELGSEMNTEN
                     DELHGVNQVVPKKRWQRLNQRRPKPGKRANRFREKENSEGAFGVLLPADAVQKAREDY
                     LEQRAPPTSKPEDSAADPNHGSHSESVAPRLNVCEKSSVGMGDVEKETGIPSLMPQTK
                     LPEPAIRSEKKRLRKPSKWLLEYTEEYDQIFAPKKKQKKVQEQVHKVSSRCEDESLLA
                     RCQPSAQNKQVDENSLISTKEEPPVLEREAPFLEGPLAQSDLGVTHAELPQLTLSVPV
                     APEASPRPALESEELLVKTPGNYESKRQRKPTKKLLESNDLDPGFMPKKGDLGLSRKC
                     FEASRSGNGIVESRATSHLKEFSGGTTKIFDKPRKRKRQRLVTARVHYKKVKKEDLTK
                     DTPSSEGELLIHRTAASPKEILEEGVEHDPGMSASKKLQVERGGGAALKENVCQNCEK
                     LGELLLCEAQCCGAFHLECLGLPEMPRGKFICNECHTGIHTCFVCKQSGEDVKRCLLP
                     LCGKFYHEECVQKYPPTVTQNKGFRCPLHICITCHAANPANVSASKGRLMRCVRCPVA
                     YHANDFCLAAGSKILASNSIICPNHFTPRRGCRNHEHVNVSWCFVCSEGGSLLCCDSC
                     PAAFHRECLNIDIPEGNWYCNDCKAGKKPHYREIVWVKVGRYRWWPAEICHPRAVPSN
                     IDKMRHDVGEFPVLFFGSNDYLWTHQARVFPYMEGDVSSKDKMGKGVDGTYKKALQEA
                     AARFEELKAQKELRQLQEDRKNDKKPPPYKHIKVNRPIGRVQIFTADLSEIPRCNCKA
                     TDENPCGIDSECINRMLLYECHPTVCPAGVRCQNQCFSKRQYPDVEIFRTLQRGWGLR
                     TKTDIKKGEFVNEYVGELIDEEECRARIRYAQEHDITNFYMLTLDKDRIIDAGPKGNY
                     ARFMNHCCQPNCETQKWSVNGDTRVGLFALSDIKAGTELTFNYNLECLGNGKTVCKCG
                     APNCSGFLGVRPKNQPIVTEEKSRKFKRKPHGKRRSQGEVTKEREDECFSCGDAGQLV
                     SCKKPGCPKVYHADCLNLTKRPAGKWECPWHQCDVCGKEAASFCEMCPSSFCKQHREG
                     MLFISKLDGRLSCTEHDPCGPNPLEPGEIREYVPPTATSPPSPGTQPKEQSSEMATQG
                     PKKSDQPPTDATQLLPLSKKALTGSCQRPLLPERPPERTDSSSHLLDRIRDLAGSGTK
                     SQSLVSSQRPQDRPPAKEGPRPQPPDRASPMTRPSSSPSVSSLPLERPLRMTDSRLDK
                     SIGAASPKSQAVEKTPASTGLRLSSPDRLLTTNSPKPQISDRPPEKSHASLTQRLPPP
                     EKVLSAVVQSLVAKEKALRPVDQNTQSKHRPAVVMDLIDLTPRQKERAASPQEVTPQA
                     DEKTAMLESSSWPSSKGLGHIPRATEKISVSESLQPSGKVAAPSEHPWQAVKSLTHAR
                     FLSPPSAKAFLYESATQASGRTPVGAEQTPGPPSPAPGLVKQVKQLSRGLTAKSGQSF
                     RSLGKISASLPNEEKKLTTTEQSPWGLGKASPGAGLWPIVAGQTLAQACWSAGGTQTL
                     AQTCWSLGRGQDPKPENAIQALNQAPSSRKCADSEKK"
     exon            1042..1177
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1350
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..3910
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            3911..4035
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            4036..4306
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            4307..4416
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            4417..4492
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            4493..4611
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            4612..4755
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            4756..4879
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            4880..5080
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            5081..5260
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            5261..5417
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            5418..5623
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            5624..5736
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            5737..6006
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            6007..6123
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            6124..6265
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            6266..6372
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            6373..6577
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     exon            6578..12784
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8451..8456
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /note="hexamer: AATAAA"
     polyA_site      8475
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
     regulatory      12762..12767
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /note="hexamer: AGTAAA"
     polyA_site      12784
                     /gene="Nsd1"
                     /gene_synonym="KMT3B"
                     /note="major polyA site"
ORIGIN      
        1 ctggaagagg cgggaattct cggggccgtc caatatggct ttctctcaac aacaaggatg
       61 cgcttaacgc tctagggacc aagtcatgcc tgaggttgat gcaggcacag tatggatcgg
      121 acttgtgaat tatctagaag aaattgtctg ctgtcctttt ctaatccggt gaatttagat
      181 gcctccgaag acaaggacag ccctttcggt aatggtcaat ccaatttttc tgagccactt
      241 aatgggtgta ctatgcagtt accgactgct gccagtggaa catcccaaaa tgcttatgga
      301 caagattctc catcttgtta cattccactg cggagactac aggatttggc ctccatgatc
      361 aatgtagaat atttaagtgg gtctgctgat ggatcagaat cctttcaaga ccctgcaaaa
      421 agtgattcaa gagctcagtc gccaattgtt tgcacttcct tgagtcctgg tggtccaaca
      481 gcacttgcta tgaaacagga gcccacttgt aataactccc ccgaactcca gttaagagta
      541 acaaagacga ccaagaatgg ctttctgcac tttgagaatt ttactggtgt ggacgatgca
      601 gatgtagatt ctgaaatgga cccagaacag ccagtcacag aggatgagag tatagaggag
      661 atctttgagg agactcagac caatgccacc tgcaattatg agcctaaatc agagaatggt
      721 gtagaagtgg ccatgggaag tgaacaagac agcatgccag agagtagaca tggtgcagtg
      781 gaacggccat tcttgccgtt agctcctcaa actgaaaaac agaaaaataa acaaagaagt
      841 gaagtggacg gaagcaatga aaagacagcc ctcctcccag cccccacctc actaggagac
      901 acgaacgtta ccgtagaaga gcaatttaac tcaataaatt tatcttttca ggatgatcca
      961 gactccagtc ccagtccatt aggaaacatg ctagaaatac ctggaacttc atcaccatcc
     1021 acttcacagg aattgccatt ttgtcaaccg aagaaaaagt ctacaccact gaaatatgaa
     1081 gttggagatc ttatttgggc aaaattcaag agacgccctt ggtggccctg caggatctgt
     1141 tctgatccat tgattaatac acactcaaaa atgaaagttg ccaataggag gccatatcgc
     1201 gaatactacg tagaggcttt tggagaccct tctgaaaaag cctgggtggc tggaaaagca
     1261 atcgtcatgt ttgaaggcag acatcaattt gaagaactac ctgtccttag gaaaagaggg
     1321 aaacagaaag aaaaaggata taggcataag gttcctcaga agattttgag taaatgggaa
     1381 gccagcgttg gtcttgccga gcaatatgat gttcccaaag gctctaagaa ccaaaagtgt
     1441 gtcagtagct cagtcaagtt ggacagtgag gaagatatgc catttgagga ctgtacaaac
     1501 gatcctgatt cagaacacct gttgcttaat ggctgcttga agtctctggc ttttgattct
     1561 gagcattctg cagatgaaaa ggaaaagccc tgtgccaagt ctcgagttag aaaaagctct
     1621 gataatataa aaaggactag tgtgaaaaag gatctggtgc catttgaatc acgtaaagaa
     1681 gaacggaggg ggaaaattcc agacaacctt ggcctagact ttatctctgg gggtgtatct
     1741 gataaacagg cttctaatga actttccagg atagcaaaca gcctcacagg gtctagcact
     1801 gcaccaggaa gtttcctctt ttcttccagt gtacagaaca ctgctaagac agactttgag
     1861 actccagatt gcgattcctt atcaggtttg tctgagagtg ctttaatctc taaacattct
     1921 ggagagaaaa agaaactcca accaggccag gtctgcagct caaaagtaca gctctgctac
     1981 gttggagctg gtgatgagga aaagcgaagc aattctgtca gtgtgagtac cacttctgat
     2041 gatggctgca gtgacctgga tcctacagag cacaactcag gattccagaa cagtgtcctt
     2101 ggaattacag atgcttttga taaaacagag aatgctttat cggtgcacaa aaatgaaaca
     2161 cagtattcta gatatcctgt cacaaacagg ataaaagaga aacagaaatc cctcattact
     2221 aattcccatg cagaccattt aatgggttct accaagacaa tggagcctga aactgctgag
     2281 ttgtctcagg tcaatctttc tgatcttaaa attagtagtc ctattcccaa accccaacca
     2341 gaatttagga atgatggtct cactacaaaa ttcagcgcgc caccaggcat tcgtaatgaa
     2401 aatccactga caaagggtgg gcttgcaaat caaactctat tacctctgaa atgcagacag
     2461 cccaagtttc gtagtataaa atgcaagcat aaagaaagcc cagctgttgc agaaacctca
     2521 gctacaagtg aggacctcag tttgaaatgc tgttcttctg ataccaatgg ctctcctttg
     2581 gccaatatat ccaaaagtgg gaaaggagag gggctaaaac tactgaacaa tatgcatgag
     2641 aaaaccagag attctagtga catagaaaca gcagtggtga agcacgttct ttcggaactg
     2701 aaggagctct cgtacagatc attaagtgaa gacgtaagtg attctggaac ggcaaaggca
     2761 tcaaagccat tactgttttc ttctgcttct agtcagaatc atataccaat tgaaccagat
     2821 tacaagttta gcacattgct gatgatgctg aaggatatgc atgacagtaa gacaaaggag
     2881 cagcggttaa tgactgctca aaatctggct tcctacagga ctccagatcg tggggactgt
     2941 tcttctggta gccctgtagg gacatcaaaa gttttggtgt taggaagctc cacacccaat
     3001 tctgaaaagc ctggagatag cactcaggac tcagttcacc agagtcctgg tgggggtgac
     3061 tctgccttgt ctggagagtt gtcttcttcc ctgtccagct tagcttctga caaaagagag
     3121 ctccctgctt gtggtaaaat tcgctctaac tgtatcccaa ggcgcaactg tggccgagca
     3181 aagccatcat ccaagttgag agaaaccatt tcagcccaga tggtaaaacc ttcagtaaac
     3241 ccaaaagcct taaagactga gcggaagaga aagttcagcc gacttccagc tgtgacactt
     3301 gctgctaaca gactgggaaa caaagaaagt ggatcagtga atggcccatc caggggtggg
     3361 gctgaagatc ctggtaaaga agagcctctg caacaaatgg accttttaag aaatgaagac
     3421 acacattttt cagatgtaca ttttgatagc aaggctaaac aatcggaccc tgataaaaat
     3481 cttgaaaaag aaccttcttt tgagaacaga aaaggcccag agttgggctc tgaaatgaac
     3541 actgagaatg atgaactcca tggtgttaat caggtggtgc ctaaaaagcg gtggcagcga
     3601 ttaaaccaaa ggcgccctaa gcccggaaag cgtgctaaca gatttaggga gaaagaaaac
     3661 tcggagggtg cttttggagt cttacttcct gctgacgctg tgcagaaggc ccgggaagac
     3721 tacttagaac aaagagctcc tcctacaagt aaacctgagg actcagcagc agatcccaat
     3781 catggcagcc actctgaatc ggttgcgcct cggctgaatg tttgtgagaa gtccagtgtt
     3841 ggcatgggtg atgtagaaaa ggagacagga attcccagtt tgatgccaca gaccaagctc
     3901 cctgagccag ctattcggtc agagaagaaa cgcctcagga agccgagcaa gtggcttctg
     3961 gaatatacag aagaatatga tcagatattt gctcctaaga aaaaacaaaa gaaggtacag
     4021 gaacaagtgc ataaggtaag ttcgcgctgt gaagacgaaa gccttcttgc ccgatgtcaa
     4081 cctagtgctc agaacaagca ggtggatgag aattctttga tttcaactaa agaagagcct
     4141 ccagttcttg aacgagaggc tccatttttg gaagggccat tggctcagtc agatcttgga
     4201 gttacacatg ctgagttgcc acagctgacc ttgtctgttc ctgtggctcc agaagcctct
     4261 ccgcggcctg cccttgagtc tgaggaattg ctcgttaaga caccaggaaa ttatgaaagt
     4321 aagcgtcaaa gaaaaccaac taagaaactt cttgaatcta atgatttaga ccctggattt
     4381 atgcccaaga agggtgacct tggcctttct agaaagtgct ttgaagccag tcgctcggga
     4441 aatggtattg ttgaatcacg ggctacatct catttgaaag agttcagtgg aggcactacc
     4501 aagatctttg ataaaccaag gaaacggaaa cggcagaggc ttgtcacagc cagagtgcac
     4561 tataaaaaag tgaagaagga ggacttaaca aaagacacac ccagctcgga gggagaactg
     4621 ctgattcaca ggactgctgc cagccccaag gagattcttg aagagggtgt agaacatgat
     4681 cctgggatgt ctgcatccaa gaaactgcag gttgaaagag gcggaggagc ggcactcaag
     4741 gagaatgtgt gtcagaactg tgagaagctg ggtgagctgc tgttgtgtga ggctcagtgc
     4801 tgcggggctt tccacttgga gtgccttggc ctccccgaga tgcctcgagg gaaatttatc
     4861 tgcaacgagt gtcacacagg aatccatacc tgttttgtat gtaagcagag tggagaagat
     4921 gtgaaaaggt gccttctacc cttatgtggg aaattttacc atgaagagtg tgtccagaag
     4981 tacccaccga ctgtcacaca gaacaagggc ttcaggtgcc ccctccacat ttgtataacc
     5041 tgccatgctg ctaatccagc caatgtatct gcttctaaag gtcgtctgat gcgctgtgtc
     5101 cgctgtcctg tggcatacca tgctaatgac ttttgtcttg ctgctggttc aaaaatcctt
     5161 gcatctaata gtatcatctg ccctaatcac ttcaccccta ggcggggctg ccgaaatcat
     5221 gagcatgtta atgtcagctg gtgttttgtg tgttcagaag gaggcagcct tctgtgctgt
     5281 gattcttgcc ctgctgcttt tcatcgagaa tgcctaaata tcgatatccc tgaaggaaat
     5341 tggtactgca atgactgtaa ggcaggcaaa aagccacact acagggagat tgtctgggta
     5401 aaagttggtc gctacaggtg gtggccagct gagatctgcc atcctcgagc tgttccctct
     5461 aacattgata aaatgaggca cgatgtggga gaattccctg ttctcttctt tgggtccaat
     5521 gactatctgt ggactcatca ggctcgagtc ttcccttaca tggaagggga tgtaagcagc
     5581 aaggataaga tgggaaaagg agttgatgga acatataaaa aagctcttca ggaagctgca
     5641 gcaagatttg aagagttaaa agctcaaaaa gaattaagac agctgcagga agatcggaag
     5701 aatgacaaga agccgccacc ttacaaacat ataaaggtga accggcctat tggcagggta
     5761 cagatcttca ctgcagattt gtcagaaatt ccccgttgca attgtaaagc tactgatgag
     5821 aacccctgcg ggatagactc agagtgtatc aaccggatgc tgctctatga gtgccaccca
     5881 acagtgtgtc ctgcaggagt tcgctgtcag aaccagtgct tcagcaagcg ccagtatcca
     5941 gatgttgaaa ttttccgcac attacaaagg ggctggggtc tccgaacaaa aacggatatt
     6001 aaaaagggag aatttgtgaa tgaatatgtg ggtgagctaa tagatgaaga agagtgcaga
     6061 gctcggatcc gttacgcaca agagcacgac atcactaatt tctatatgct cacgctagat
     6121 aaagatcgaa tcattgatgc tggtcctaaa gggaactacg cacgcttcat gaatcactgc
     6181 tgccagccta attgtgagac acagaagtgg tcggtgaatg gagatacccg tgttggtctt
     6241 tttgctttga gtgacattaa agcaggcact gaacttacct tcaattacaa cctagagtgt
     6301 cttgggaatg gaaaaaccgt ctgtaaatgt ggagcaccca actgcagtgg cttcttgggt
     6361 gtgaggccaa agaatcaacc cattgtcaca gaagaaaagt caaggaaatt caaaaggaag
     6421 ccacatggga agcgcaggag ccagggcgaa gtcaccaagg aaagagagga tgaatgtttc
     6481 agctgtgggg atgctggtca gctcgtctcc tgcaagaagc caggctgccc taaagtctac
     6541 catgcagact gtctgaatct gacaaagcga ccagcaggga agtgggaatg tccttggcac
     6601 cagtgtgacg tgtgtgggaa ggaagcagcc tccttctgtg agatgtgccc cagctccttt
     6661 tgcaagcaac atcgagaagg gatgcttttc atttccaagc tcgatgggcg tctgtcttgt
     6721 actgaacatg atccctgtgg gcccaaccct ctggaacctg gggagatccg tgagtatgtg
     6781 cctcccacag cgacgagtcc tccaagcccc ggcactcagc caaaagagca gtcatcagag
     6841 atggctactc aggggcccaa gaagtctgat cagccaccta ctgatgccac ccagttgctg
     6901 ccactctcca aaaaagccct aacagggtct tgtcagaggc cactgctccc tgaaaggcct
     6961 cctgagagaa ctgactctag ttcccacctt ttagatagga tcagagacct tgctgggtca
     7021 gggaccaaat cccagtcctt ggtatccagc cagagaccac aggacaggcc acctgctaaa
     7081 gaaggaccaa gacctcagcc acctgacaga gcctctccaa tgaccaggcc aagctcatca
     7141 ccttcagtca gctccctgcc actggaaaga cctctaagaa tgactgactc caggctggat
     7201 aaatccatag gtgctgccag cccaaagtcc caggcagtgg agaaaactcc agcctccact
     7261 ggcctgagac tttcatcacc agacaggctg ctaaccacta acagtcccaa accccagatt
     7321 tctgacaggc ccccagagaa gtcccatgcc tctttgaccc agagacttcc acctcctgag
     7381 aaagtgctat cagctgtggt ccagtctctg gtagctaaag agaaagccct gaggcctgtg
     7441 gaccagaaca cccaatcaaa gcacagacct gctgtggtga tggatctcat agacctaacg
     7501 cctcgccaga aggagcgggc tgcttctcct caggaagtca ccccacaggc tgatgagaag
     7561 acagcaatgc tggagtcaag ctcatggccg tccagcaaag gtctggggca tatcccacga
     7621 gctacggaga agatcagcgt gtcagagtcc ctccagccct cgggaaaagt agcagctcct
     7681 tcagaacacc cttggcaagc tgttaaatcg ctcacccatg ccagatttct ttctccgcct
     7741 tcggccaagg cttttttata tgaatcagca actcaagcct caggaagaac ccctgtggga
     7801 gctgagcaga ccccaggacc tcctagccca gcaccaggcc tggttaaaca ggtgaaacag
     7861 ctatctagag gactcactgc caagagtggg cagtccttca ggtctcttgg gaagatctca
     7921 gcttccctcc ccaatgaaga aaagaagttg acaaccacag aacagagtcc ctggggcctg
     7981 ggaaaagcct caccaggggc agggctttgg cctatagttg ctggacaaac actagcccag
     8041 gcttgctggt ctgctggggg cacacaaact ttggcacaaa cttgctggtc tcttggaaga
     8101 gggcaagacc ccaaaccaga aaatgcaatt caagctctta accaggctcc ttctagtcgc
     8161 aaatgtgcag attcagaaaa gaaataatac agtaaatgtc acataactag acaagctgtc
     8221 cccagggtac catttgagga gggaaatctt catttctttt tccttcttga aaaacaaaca
     8281 tattcacact tccactgttc ttctttcctt atatctcaac actcagaact cttttgacat
     8341 tagcccatgg ggacttgtgg ttgtgaaccg tgtatgggaa gtcgctgggc cccagtcaag
     8401 gagacagact tgagtctctt tgccctaact tttacatatg gtcaacttaa aataaaaagt
     8461 ccactctgga atcaagaatg gaattcgatt ccactggtca acttggaaag tgtaggggct
     8521 ctcaaggcta tttccctagc cgaaccgtgt cccattgaga accctgtaaa cccttttgga
     8581 gaacctggtg ttcattcaca tcactggcag tttcctgaat attgatatgt gcaagggcag
     8641 ggggccagtc attattgtga gacagaatta cataaggctc gatcccaaag agcctaactt
     8701 gcctgcttgg gtccccagat ttcaaggacc tttgaccagg gaacaacaga aagtacttat
     8761 aggccatggg tttccagttc tgttttcaag tggtttgtta atgttttggg ttttttgttt
     8821 ttttgttttt tttttgtttt tgtttggggg gagggtgtct tttgtttttg ttttgtggcc
     8881 cagagtgtgg ttatatagaa catacatctc ctggttggaa catgcatgca tgtatgtgca
     8941 tgtgtgtgcc cacaaagctc ttctgtaaat ggaagggtac tacatcaagg ccaaggtgag
     9001 cttttggctg gggcaactgc tgcagtctct gtgttcttgt ttgaagattt gtccttggag
     9061 gagcatctgg ttagagggag atgttctctg agaatgtagt gtcccagaag tgaacaaggg
     9121 cgattggcaa gcttaccttt cttttctacc aataatcctc tcacaagaac ccccatcctc
     9181 atttcctcaa acactcaggt gctttcagat ttcagtctcg ggcagtggac atagggaatt
     9241 tatggcaagc tctgagcaag acataccacc ttcaaggtga gtgtcagatt ccaaggaaat
     9301 tctttcctgg tcttgtgccc tcctacctga ggggaggaga gaattcctac ctgaaaagag
     9361 agggccctct ccacttccct gcaaatgact gctctctgta atagagacca catgcctggt
     9421 aacttagctc agtggcaagc caaaaccctt ccccaagact ggagagacgt gatgtttgga
     9481 gcaatgtcca atctaagcaa tgactcctca taactgctga ttatctgtta ctgctgcctg
     9541 agctggggcc caagggctgg gaatctgttg gtgctacctt gccctaccat ttccccagct
     9601 cacaaactgc caacaggaag tgctgtttgg ctagttgtac cacactcacc tgccctttgt
     9661 tctcagatca tgtgtttgtt tacctgtcag ctttgccaac tcagcatctt tagcaaacag
     9721 acagcatctt ttggcctttc tgactcagtt acagctttta caccctcaac aggagtctgt
     9781 atattcccca gtttccttct ccctaagttc ctgttgaggt tagcttccat ctcttcttga
     9841 ggaaagaaca gtggttatat tagcatttgc gctttatata aaaattgaaa gtaggatctg
     9901 cttttatttc ccaaagcctg tctgtggaat tgagaccctt gaactcaggc agagggatga
     9961 ggctggggca gggctgcccc aagtttgggg gcctaatccc tgcacttccc tgaaacctca
    10021 gaatctcccc tctgaataca cctgcctagt tctctcccct ttcatcctct cttcccacat
    10081 cgtcagagtg aaaaaaagct ctttgtttta aaggaagaga aaaagcgtct tattttcttt
    10141 ttaaaaaatt ttaaaccatg aagaaaatat ttttaaagaa attcacccag gacattaaag
    10201 ttcactatat tacctattta tcatgtgtga gaatgtaagt atatacctgc agctagtaga
    10261 cgcctccctc ctttctttcc tccctccctc ctttccttcc ctcatttctt aggctgtgtg
    10321 aataggtttg cttggtgcca gtcctgtgcc ctttgctctc ctgtcatctg tagttgtgat
    10381 cagaaagata cctgcactgc actgtcagag tctcctttca ctatgttgtg tgttcgatta
    10441 ccgtagctgt ttcatgaaat aaactgtgaa gtggggggtg gggggtgcag actatgtaaa
    10501 aattttaagt gaagaagttt ggccttgacc tagcttctct acaataggtt gagtaggtaa
    10561 ttggctttga ccattgtcta gaagtgtctc acaggacagg agggagtgag ttgggggcac
    10621 aattggccac ttgtcttttg ttttgatact tccattcacc gacattcttt tggagccttc
    10681 cttccagacc tgctgggaga acgtcatctg cgccctgtac ttggtctgga ggggagggat
    10741 tgggggatgc aggggaagat cagtccttct gtcctttgtt accactgaga gactcggtgg
    10801 aacctcattg acagcatcat aaggatagtg ttagaactgg gcactggtta ctatacctta
    10861 gtaacacaag tattggggtg gagaaccttg cagttccttc tggactgctt ttggatcctg
    10921 taataacctt ttcttcaagg gtgtgaattg ccagcagaga aataggtggt aattttggct
    10981 ttggctaagt gcttaaattt tggatgacca gagagtctgc acatgagaca gctcccagga
    11041 agtagtttcc agggacaagc caaaggcaga aatgttcaca ggtcataact aatacttctc
    11101 tcgtttagtt gtaggggagg gaatataagg aaattgttgc aatttgcatt caagacttaa
    11161 tttttcttcc tgctggagcc aacccatttt aatagatggt aagccatcat ttaccaccac
    11221 cacaccctca aagtattgtt aaatgtctgc ttccctaaaa ttactgagtt tgagattatt
    11281 taagtcacta tgtcaggctt ttttttttct tttttctttt tttttaccag taatgcatct
    11341 ttggtactta ttctctaccc cacactacag tctgggcttg aagtacagtg gtgagccttt
    11401 gtggccctgc actgagttca cgaacgctgg acactggtcg tgtcccagtg ttgttctaga
    11461 actgacggga aggatgtcta cgttgtccat tcagggcact caggagagca ttcaatttga
    11521 tatacacaca cgccctccaa gctgaggtac tgtcatcccc ttggagtttg tcattggatg
    11581 agaagaggcc cattgttctc acacctcctc gaccaccggg caggagtggc cgcagggatt
    11641 tactgctgtt aaaatggagc tattcatgtt cattatccca agtcatttag acctaggtag
    11701 ccactgcagg acctttgatt ggctgtgacc agttcactct cagttgagct tcctaggtca
    11761 gtacaggagt tgctatagta taaattgaaa caaaataatt gctttgtaca caatacaatg
    11821 taatgatggt gctaatctca tggtataaat aagcactgcc aagggttgag ggagtggtga
    11881 caggagaaac ccaggttcct gttgggaaga tagtttatat aggttccttt ctgctctaag
    11941 aggagggaat caggcagtca gtaatgcctg ccaacccaac cctggagtga ttcagtccag
    12001 tccagaggag gaagagaccc tgtgtccaga tgatggcact gaatttctta tggctagtat
    12061 cgccttgacc atagctttta tctgtctatc ctgcccagga aaggtcagaa gagaactgtg
    12121 ggcgtggagt agccattacc ttctgcttct ctacctcctc ccctctcatg gcagcatctc
    12181 aattgcttat gaagaataag ggcaatgttt aagaattttg gaagagcttt gttagacaac
    12241 aaacaacctc aaaagagatg tttcctgacc attataagca aaatgcatta aactcttttg
    12301 tggttcttcc cctataacag ccttcctgtg actttaagaa cataagtcta cgagaggccc
    12361 taggagttgc tcctgggagc ttagtgcacc ttggcagaag cagacaggga gcagagcaga
    12421 aactgaggtg ttggcattgt ttctaggttg ggcagagcag gtggtctcca ggttgtggtg
    12481 gtgccgccgt gtgtgtatgt aggagagtgt ggaggctggg tgagtacagc atctaggagc
    12541 tcgtgggaaa gcagctttct aggtctagca aagaaaactg actagaaagc tatatttttt
    12601 aaatggggaa aagggttcaa cgaaggtccg tcactgtgtt ctctcgcccc caacccgttg
    12661 ccagctggaa gtgaacctag tgaaaaagag gggagccctg tgctaggagt ccccataaac
    12721 atgtactgta attctttgta tatagaaaaa aattactgta aagtaaagtt taactttact
    12781 ctta
//
