LOCUS       NM_020252               9031 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus neurexin I (Nrxn1), transcript variant 1, mRNA.
ACCESSION   NM_020252
VERSION     NM_020252.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Wutikeli,H., Yu,Y., Zhang,T., Cao,J., Nawy,S. and Shen,Y.
  TITLE     Role of Elavl-like RNA-binding protein in retinal development and
            signal transduction
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1871 (1), 167518 (2025)
   PUBMED   39307290
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   Cole,N., Harvey,M., Myers-Joseph,D., Gilra,A. and Khan,A.G.
  TITLE     Prediction-error signals in anterior cingulate cortex drive
            task-switching
  JOURNAL   Nat Commun 15 (1), 7088 (2024)
   PUBMED   39154045
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9031)
  AUTHORS   Brockhaus,J., Kahl,I., Ahmad,M., Repetto,D., Reissner,C. and
            Missler,M.
  TITLE     Conditional Knockout of Neurexins Alters the Contribution of
            Calcium Channel Subtypes to Presynaptic Ca2+ Influx
  JOURNAL   Cells 13 (11), 981 (2024)
   PUBMED   38891114
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9031)
  AUTHORS   Yan,Q., Weyn-Vanhentenryck,S.M., Wu,J., Sloan,S.A., Zhang,Y.,
            Chen,K., Wu,J.Q., Barres,B.A. and Zhang,C.
  TITLE     Systematic discovery of regulated and conserved alternative exons
            in the mammalian brain reveals NMD modulating chromatin regulators
  JOURNAL   Proc Natl Acad Sci U S A 112 (11), 3445-3450 (2015)
   PUBMED   25737549
REFERENCE   5  (bases 1 to 9031)
  AUTHORS   Iijima,T., Wu,K., Witte,H., Hanno-Iijima,Y., Glatter,T., Richard,S.
            and Scheiffele,P.
  TITLE     SAM68 regulates neuronal activity-dependent alternative splicing of
            neurexin-1
  JOURNAL   Cell 147 (7), 1601-1614 (2011)
   PUBMED   22196734
  REMARK    GeneRIF: Findings uncover SAM68 as a key regulator of dynamic
            control of Nrxn1 molecular diversity and activity-dependent
            alternative splicing in the central nervous system.
REFERENCE   6  (bases 1 to 9031)
  AUTHORS   Tabuchi,K. and Sudhof,T.C.
  TITLE     Structure and evolution of neurexin genes: insight into the
            mechanism of alternative splicing
  JOURNAL   Genomics 79 (6), 849-859 (2002)
   PUBMED   12036300
REFERENCE   7  (bases 1 to 9031)
  AUTHORS   Geppert,M., Khvotchev,M., Krasnoperov,V., Goda,Y., Missler,M.,
            Hammer,R.E., Ichtchenko,K., Petrenko,A.G. and Sudhof,T.C.
  TITLE     Neurexin I alpha is a major alpha-latrotoxin receptor that
            cooperates in alpha-latrotoxin action
  JOURNAL   J Biol Chem 273 (3), 1705-1710 (1998)
   PUBMED   9430716
REFERENCE   8  (bases 1 to 9031)
  AUTHORS   Missler,M. and Sudhof,T.C.
  TITLE     Neurexins: three genes and 1001 products
  JOURNAL   Trends Genet 14 (1), 20-26 (1998)
   PUBMED   9448462
  REMARK    Review article
REFERENCE   9  (bases 1 to 9031)
  AUTHORS   Andres,C., Beeri,R., Friedman,A., Lev-Lehman,E., Henis,S.,
            Timberg,R., Shani,M. and Soreq,H.
  TITLE     Acetylcholinesterase-transgenic mice display embryonic modulations
            in spinal cord choline acetyltransferase and neurexin Ibeta gene
            expression followed by late-onset neuromotor deterioration
  JOURNAL   Proc Natl Acad Sci U S A 94 (15), 8173-8178 (1997)
   PUBMED   9223334
REFERENCE   10 (bases 1 to 9031)
  AUTHORS   Puschel,A.W. and Betz,H.
  TITLE     Neurexins are differentially expressed in the embryonic nervous
            system of mice
  JOURNAL   J Neurosci 15 (4), 2849-2856 (1995)
   PUBMED   7722633
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC151286.4, AC167814.4,
            AC171209.1, CT025708.8, AC101872.7 and CT486002.9.
            
            On Mar 29, 2022 this sequence version replaced NM_020252.3.
            
            Summary: This gene encodes a single-pass type I membrane protein
            that belongs to the neurexin family. Neurexins are synaptic
            transmembrane receptors that bind endogenous ligands that include
            neuroligins, dystroglycan, and neurexophilins. Neurexin complexes
            are required for efficient neurotransmission and are involved in
            synaptogenesis. In vertebrates, alternate promoter usage results in
            multiple isoform classes, of which the alpha and beta classes are
            the best characterized. In humans, allelic variants in this gene
            are associated with Pitt-Hopkins-like syndrome-2, while deletions
            have been associated with autism and schizophrenia. Mouse knockouts
            display decreased spontaneous and evoked vesicle release resulting
            in impaired synaptic transmission. In addition, knockout mice show
            altered social approach, reduced social investigation, reduced
            locomotor activity, and in males, increased aggression. Alternative
            splicing and promoter usage result in multiple transcript variants.
            [provided by RefSeq, Nov 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB093249.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-195               AC151286.4         10078-10272
            196-1846            AC151286.4         13266-14916
            1847-1864           AC151286.4         107393-107410
            1865-1894           AC151286.4         110727-110756
            1895-1906           AC151286.4         111493-111504
            1907-2208           AC167814.4         36150-36451
            2209-2232           AC167814.4         38506-38529
            2233-2394           AC167814.4         39612-39773
            2395-2833           AC171209.1         211021-211459       c
            2834-3217           AC171209.1         197915-198298       c
            3218-3421           AC171209.1         191450-191653       c
            3422-3448           AC171209.1         188895-188921       c
            3449-3571           AC171209.1         165537-165659       c
            3572-3953           AC171209.1         157878-158259       c
            3954-4144           AC171209.1         156701-156891       c
            4145-4318           AC171209.1         133335-133508       c
            4319-4438           AC171209.1         128771-128890       c
            4439-4620           CT025708.8         165520-165701       c
            4621-4792           AC101872.7         7029-7200
            4793-4882           AC101872.7         51642-51731
            4883-5202           AC101872.7         53090-53409
            5203-5290           CT486002.9         91250-91337
            5291-9031           CT486002.9         113429-117169
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 59.73 cM"
     gene            1..9031
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /note="neurexin I"
                     /db_xref="GeneID:18189"
                     /db_xref="MGI:MGI:1096391"
     exon            1..195
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            196..1846
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1864
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..1894
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..1906
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..2208
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            2209..2232
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2394
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2833
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            2834..3217
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            3218..3421
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            3422..3448
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            3449..3571
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            3572..3953
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..4144
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            4145..4318
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            4319..4438
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            4439..4620
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            4621..4792
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            4793..4882
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            4883..5202
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            5203..5290
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     exon            5291..9031
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8702..8707
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /note="hexamer: AATAAA"
     polyA_site      8723
                     /gene="Nrxn1"
                     /gene_synonym="1700062G21Rik; 9330127H16Rik;
                     A230068P09Rik; mKIAA0578"
                     /note="major polyA site"
ORIGIN      
        1 ctctcctcct cctgctcccc ctgctgctcc tctctgttcc atgttcttcc cttcctcact
       61 cttggagata gacgctcaag cctgacgcct cggaaaaagg cagcagcagt cgccttatcc
      121 ttagacctca ggaaattcgc ttttaccgat gcatgcttca ggttctggct cgagctaagg
      181 ggaataacca ggcagatttc tgagttctta taaacgtcaa aataaaatca aaatgcatca
      241 ccaacataca accaactgac atgctggagt cttagggtgg ccttgcagag acagaaaaaa
      301 gaagagtttg agggattgat ttcaccatct aacagcttct ggctacttca cagcagggtt
      361 tgagacctac ctacagggct cctaaacata aagcgaatca gcctgggagg gcatgcacag
      421 gaaatttggc cttggcttta gtggtgctgg aagcccatga tatgacagaa gtaggcctct
      481 gaagctattc tggtgtctca ctctgtcctt tctccttctg tgcttggagc caacatctgg
      541 aggggcctcg aggcctgcca agcagccaaa gatcctggct cagtgttgac ttcgtgttcc
      601 tctccgagaa aagtgtgttt cttccgattc tgctctccta ctgctggcac cgcgccggtg
      661 gtactttgaa ggttttggtc ctgggatagt tgaagtacct gttggtgcca cagatgccct
      721 cacttaaaca tcatcggatg caaatggatc agtgatcgct cttgagcctc ggtggccctc
      781 tttttcagaa cgttgcctcc aaagtgtatc ccccctttgg cctggaagtt ctgcagctat
      841 cccataggac tggggtctcc gctttccagt tggaaggaga ctactgggag agaggaaggg
      901 gctgctggaa gagaagaaga aggaaaaaag agctgaagga gggtgcgccc ctcccactgc
      961 cgcctccccc ctccctccca ctcctctgcg gacccccatt tctctgtgaa ggtgtccagg
     1021 accgcttcga ccctgtcgcc cccggctccc ccccgccgca ccccagcccc gagcatgggg
     1081 acggcgctgg tccagcgcgg gggctgctgt ctcctctgcc tgtcgctgct gctgctgggc
     1141 tgctgggcag agctgggcag cgggctggag ttcccgggcg ccgagggcca gtggacgcgc
     1201 ttccccaagt ggaacgcgtg ctgcgagagc gagatgagct tccagctgaa gacgcgcagt
     1261 gcccgcggcc tcgtgctcta cttcgacgac gagggcttct gcgacttcct cgagctcatc
     1321 ctgacgcgcg gcggccgcct gcagctcagc ttctccatct tctgcgccga gcccgccacg
     1381 ctgttggccg acacgccggt caacgacggc gcctggcaca gcgtgcgcat ccgccgccag
     1441 ttccgcaaca ccacgctcta catcgaccgc gccgaggcca agtgggtgga ggtcaagtcc
     1501 aagcgcaggg acatgacggt gttcagcggg ctcttcgtgg gcggcctgcc acccgaactg
     1561 cgcgccgcgg ccctcaagct cacgctggcc tcggtgcgcg agcgcgagcc cttcaagggc
     1621 tggatccgtg acgtccgggt caactcgtca caggccctgc cggtggacgg cggcgaggtc
     1681 aagctggacg acgagcctcc caacagcggt ggcgggagcc catgcgaggc gggcgaagaa
     1741 ggcgagggcg gcgtgtgcct caacgggggc gtgtgctccg tggtcgacga ccaggccgta
     1801 tgcgactgct cgaggaccgg cttccgtggc aaggactgca gccaagaaga caacaatgtg
     1861 gaaggtctgg cacacctgat gatgggcgac caaggtaaaa gtaaaggaaa agaagagtat
     1921 attgccacgt tcaagggatc tgaatacttc tgctacgact tatctcaaaa ccccattcaa
     1981 agcagcagtg atgaaataac tctgtccttt aaaactcttc aaaggaacgg actgatgctt
     2041 cacacaggga aatcagctga ttatgtcaat cttgcattga aaaatggagc tgtctctctg
     2101 gtcattaatt tgggatcagg ggcctttgaa gcactagtgg agcctgtgaa tggaaagttt
     2161 aatgataatg cctggcatga tgtgaaagtc accaggaatc tgcgtcagca ctcaggcatt
     2221 ggacacgcta tggtgacaat atcagtggat ggaattctta ccacaacggg ctacacgcaa
     2281 gaagattaca ccatgctggg gtctgatgac tttttctatg ttggaggcag ccccagcaca
     2341 gccgaccttc cagggtcacc agtcagtaac aactttatgg gctgtctcaa agaggttgta
     2401 tataaaaata atgatgtaag gctggaatta tcgcgacttg ccaagcaagg agatcctaag
     2461 atgaagattc acggtgtggt ggcttttaag tgtgaaaatg tggcaacctt agacccaata
     2521 acttttgaga ccccggaatc tttcatttct ttgcctaagt ggaatgcaaa gaaaacaggc
     2581 tcaatatcat ttgatttccg tacaacggag ccaaatggcc tcatcctgtt tagccatggc
     2641 aagccaagac atcagaagga tgccaagcac ccacagatga ttaaggttga cttttttgct
     2701 attgagatgc tggatggcca cttgtacctc ctcttagaca tgggatcagg tactataaaa
     2761 atcaaagccc tgcagaagaa agtcaatgat ggagaatggt atcacgtgga tttccaacgg
     2821 gatggacggt caggtaccat ttctgtcaac acactacgca ctccctacac agctcccggt
     2881 gagagtgaga tcctggacct agatgatgag ttgtacctgg gtggcttgcc agaaaataag
     2941 gctggcctag tcttccctac cgaggtgtgg actgctctgc tcaactacgg ctacgtgggc
     3001 tgcatccggg acctatttat tgatggccag agcaaagaca tccggcagat ggcggaaatt
     3061 cagagcactg ctggagtgaa gccatcctgc tcaaaagaga cagcaaaacc gtgccttagc
     3121 aatccttgca aaaacaatgg catgtgcaga gatggctgga acagatacgt ctgcgattgc
     3181 tccggaacag gctatctcgg caggtcctgt gagagagagg caacggtttt gagctacgac
     3241 gggagcatgt tcatgaaaat ccagcttccg gtggtgatgc acactgaggc tgaggacgtg
     3301 tccttgcggt tccgatccca gcgtgcatat ggtatcctga tggcgaccac ttctagagac
     3361 tctgcagaca cccttcgatt ggagctagat gcaggacgtg tgaaactcac ggtcaatcta
     3421 gattgtatca ggattaactg taattccagc aaaggtcctg agaccctttt tgctggctat
     3481 aacctcaatg ataatgaatg gcacacagtg cgtgtggttc ggcgaggaaa aagtttaaag
     3541 ctaaccgtgg atgatcaaca agccatgaca ggccaaatgg caggtgatca tacaaggctg
     3601 gagtttcata acatagagac aggcatcatc accgaacgac gctacctttc ttctgtcccc
     3661 tccaatttca ttgggcacct gcaaagcctg acatttaatg gaatggcata catagacttg
     3721 tgcaaaaatg gtgatataga ttactgtgaa ctcaatgcca ggtttggatt caggaacatc
     3781 atcgcagatc ctgtcacctt caagaccaaa tcgagctatg ttgccttagc tacattgcaa
     3841 gcctacacct ctatgcatct atttttccaa ttcaagacaa catctctaga tggactaatt
     3901 ctgtataaca gtggggatgg aaatgacttt attgtggttg aattagttaa agggtactta
     3961 cattatgtgt ttgacttggg aaacggtgct aacctcatca aagggagctc aaacaaacca
     4021 ctcaacgaca atcagtggca caatgtgatg atttcacggg acaccagcaa tctccacaca
     4081 gtaaagatcg acacaaaaat cacaacacaa atcactgcgg gagccagaaa tctagacctc
     4141 aagagtgact tgtatattgg aggagtggct aaagaaacat ataaatccct gccaaaactg
     4201 gtccatgcca aagaaggctt tcaaggttgc ctggcatctg tggacttgaa tggcagactt
     4261 ccagatctca tctcggatgc tctcttctgc aatgggcaaa ttgagagagg atgcgaaggg
     4321 cccagcacaa cctgccaaga ggattcatgt tcaaatcaag gcgtttgctt gcagcaatgg
     4381 gatggcttca gctgtgactg tagcatgact tccttcagtg gcccactctg caatgaccct
     4441 gggacaacat atatctttag caaaggtggt ggacagatta catataagtg gcctcccaat
     4501 gaccgcccca gtacacgagc agacaggctg gccatcggat ttagcactgt ccagaaggaa
     4561 gccgtgttgg tgcgtgtgga cagttcctca ggactgggtg actacctgga gctgcacata
     4621 caccaaggaa aaattggagt taagtttaat gttgggacag atgacatcgc catcgaagag
     4681 tctaatgcaa tcattaatga tgggaaatac catgtagtac gtttcacaag gagtggtggc
     4741 aatgccacgt tacaggtgga cagctggcca gttatcgaac gctaccctgc aggaaacaat
     4801 gataacgagc gcctggcgat tgctagacag cgaattccat atcgacttgg tcgagtagtt
     4861 gatgaatggc tactcgacaa agggcgtcag ctcacaatct tcaatagcca agcaaccata
     4921 ataattggcg ggaaagagca gggccagccc ttccagggcc agctctctgg tctttactac
     4981 aatggcttga aagttctgaa tatggcagca gagaacgatg ccaacatcgc catagtgggg
     5041 aatgtgaggc tggtcggtga agtgccttcc tctatgacaa ctgagtcgac agccactgcc
     5101 atgcagtctg agatgtccac ctcaatcatg gagaccacca caaccctggc taccagcaca
     5161 gctagaagag gcaagccccc cacaaaggaa cccatcagcc agaccacaga tgacatcctt
     5221 gtggcctcgg cagagtgtcc cagtgacgat gaggacattg acccctgtga gccgagctca
     5281 ggtgggttag ccaaccccac cagagtaggt ggccgtgaac catacccagg ctctgcagag
     5341 gtgattcggg agtccagcag taccactggc atggtggtgg ggattgtcgc agcagccgct
     5401 ctgtgcatcc tcatcctcct ctatgccatg tacaagtaca ggaaccggga tgaagggtcg
     5461 taccacgtgg atgagagtcg aaactacatc agtaactcag cacagtccaa tggggctgtg
     5521 gtcaaggaga agcaacccag cagtgctaaa agcgccaaca aaaacaagaa aaacaaggat
     5581 aaggagtatt acgtctgata tcaagatttg aaacggacac ttgtatagaa atagtcttca
     5641 ttttatctga gacataatat aaacttttac tttccttttt atgaagcaca tacaaaagaa
     5701 gacagggaat gcgatcagga gggaaaactc tttttaaaaa aaaaaacaaa caagtatctc
     5761 atgctcttgt ttctcaaaag aaaaaaaaaa catacacaaa aaaaaaaaag aaagaaagag
     5821 agaagaaagg aaggaaaaaa tactaaaaca aaaacagggg ccaataaaat tccctaacat
     5881 ccgcagtgtt ttcatttact ctgcttgtct ttatgttgct gggacgttct aaaagacggt
     5941 gaggaccgca cgcattcata aagcaaagga gtattacatc gaggcacaac acaaaaccaa
     6001 cacaaaacac aacacacaca aagaagctac ctatgatcct ggattcagcc aaagtgctag
     6061 cgctttcctg aggagccagt cagtgggcgt gccagagaag aagtcttctt atgggaccat
     6121 ctgcagatta tgaggagttg gtcagctggc acaactggag caggggttgg aacttgcatt
     6181 tgagacaaag tgctggcttt ttttttcttt ttttcttttc ttttcttttt cttttttttg
     6241 aagactaatg caggaaagcg tttttaaaaa gcccctttct gtttgtgaga gagtaacaat
     6301 taaaaaaaat attatggagg ccttattttt caaaatgtga aatataaggc acatgttcac
     6361 actaaaatta caaaacaaaa atgagagggc atagatgcaa tcattgggaa attttcatgc
     6421 acgcttacta tgttattaca tatgtttata taaacccatc tctgtgtgct ttctggactg
     6481 tgataagtga cgttttatag cctgttgtat agaaaatgca aaatatatct ctgctcttca
     6541 gccatttttg gtaaattcaa tgttataagt gttgctaagt atagggagtt ttatgacatc
     6601 ggagcaacaa ttatttcaga cagatttttt tgccgacatt ataaattgcc acaattactt
     6661 acttttttaa actaaaatta cagtgtagtg tttattctaa gaaagatatg tatgaatgta
     6721 tataaaaaga ctcagctact tcttttctta catatatagc cttcattctg ttgcattatt
     6781 aagttttagt aattgtatga aaggtgtgaa ttagaaggtg aaatatatac ctaagtatca
     6841 gataaaactt tctctttccc aaatactcat atgtctatat gtgtttaaca ttcacacaca
     6901 tacacacaca cacacacaca cacacacaca cacacaaata cacatacaca ggcagccatc
     6961 agatatgatg gaataccaaa acacacaaat actgacaaat tgaaaatgtg aagttagaca
     7021 gttgtgccaa ggtattataa caaatcggtg atttgcttca ttgagattct gattccagga
     7081 gaccttgaat tcttagtccc tagtaaaatg gaacgtttat tttaaacgaa agagatccat
     7141 caccatacat ggctgcataa atatgcatca ctcaacaggc attacataaa taatgttggt
     7201 ttactctggc tgctgcaatg gaaaacattt ttcccataaa tttatagata ttctctgaac
     7261 atggtgtgtg tgatcaagct ttatggaaag taagcatttt ctcaactaaa aaagtattct
     7321 cagtaatttt tgattctgta ttactaccca ctaaaatcat tctctatttg ttgactattg
     7381 acttaatttt aacttttctg gttttacatc tatatactta aacatagttt tagtttaaca
     7441 cacactgtgt agtagtgaag tataggttac tcacttgttt tgcataatgt ttgaatgatg
     7501 ttcatagcaa taaattatca aggtgagaaa ataattttta ggttgcaggg tgatgcctct
     7561 gtaaaaattg atcacatgac ataaacatct aaaagaataa ttcttggttt cataaaagag
     7621 aaagatatca ctaatctgct ttaatcaaca aggcaaaaaa atgcaaaaca tcttttgtga
     7681 taatttagtt gatgaaaaac atgccgagat gtggcctgga tttcagacac aacactacct
     7741 tagaaataca tagatatcat agcaaatcac tatgaggtac agctgccaag aaattgtgta
     7801 ataagtctta aaggcttttc atcacccaaa aaatagatgg cacgaagtta aaattcagta
     7861 cccatttctt ctctttcaag tcttcatata tatttaattt gccaggttaa aagatcttac
     7921 aagtcttttt acctacccct ctcattcagt cttactcagg aaaagccaga gaaataaaaa
     7981 ggaacataaa accaccaatt tacatatcta tattgatata gatagaaata tatattccat
     8041 tgtatctatt taacataaac tagtttcatc tgaacagatt ataaaagatg gtggaaaact
     8101 aaattgaatt gttacttttt aaatggtata tctgggaggc ttgttgttta aaattgattt
     8161 cagtgtttct tcccagcaag aaatttaaaa tctcattcat tgataaaaac tgataatgct
     8221 ggtaaaaaat atgtatctaa ttttactgac ctacacaaaa cttagcagga ttcccccccc
     8281 aacccccccc cccccgaatg tagaaggcaa agagaggatg ctgatgtttt gaaggaaaaa
     8341 gacttgaatc caacccaagt atctttatac taaacatatc aggttccgtg tgtcacttgc
     8401 agctcacaga agttttcaca ctacctggca taaactgact acatcgattg taacattttt
     8461 gtttgagtat gcttcttttt ttatccatgt aatgtgttgc ctttaaaaac aaaaatgatg
     8521 actaaaataa tgtgtgaggt ggttcatccc taagaagtca acggaaggtc acttcaatcg
     8581 tacccttcta gtgcttctaa tgactgaata tctaaggggt ttgttttgtt ttgtttttct
     8641 gttgcaaggc caatttatcc attattggaa atgctaaaca agtggaattt actgttttgt
     8701 taataaaata tttcttaata caactgtggt ttgttgactt taaaaattgc acatgacagc
     8761 aatttgccac tgaatataac aatgaagaag aatgcatttg aaaggagatc ttatccccag
     8821 acacacacag gaaaagatgc tgtcacaccc ctttcagcat tttactactt aatcagtccc
     8881 tctgcttgta cacttaagat gcaggcactc catgtagctt tcatggaaaa ttacagaact
     8941 agcagagaat aatacgaata tatgctgatg tcataaatac actctctgta agttacaatg
     9001 ggattaaagt taaatacacc tgtaattaac t
//
