LOCUS       NM_176930               7572 bp    mRNA    linear   ROD 11-DEC-2024
DEFINITION  Mus musculus neuronal cell adhesion molecule (Nrcam), transcript
            variant 1, mRNA.
ACCESSION   NM_176930
VERSION     NM_176930.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Shigesawa,I., Nakamura,A., Yamauchi,Y., Kawata,S., Miyazaki,A.,
            Nomoto,H., Kameda,H., Terauchi,Y. and Atsumi,T.
  TITLE     Effects of glucokinase haploinsufficiency on the pancreatic
            beta-cell mass and function of long-term high-fat, high-sucrose
            diet-fed mice
  JOURNAL   J Diabetes Investig 15 (12), 1732-1742 (2024)
   PUBMED   39305123
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   LeFever,N.M., Katreddi,R.R., Dolphin,N.M., Mathias,N.A. and
            Forni,P.E.
  TITLE     Following the p63/Keratin5 basal cells in the sensory and
            non-sensory epithelia of the vomeronasal organ
  JOURNAL   Genesis 62 (2), e23596 (2024)
   PUBMED   38665067
REFERENCE   3  (bases 1 to 7572)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 7572)
  AUTHORS   Zhou,L., He,L., Liu,C.H., Qiu,H., Zheng,L., Sample,K.M., Wu,Q.,
            Li,J., Xie,K., Ampuero,J., Li,Z., Lv,D., Liu,M., Romero-Gomez,M.,
            Hu,Y. and Tang,H.
  TITLE     Liver cancer stem cell dissemination and metastasis: uncovering the
            role of NRCAM in hepatocellular carcinoma
  JOURNAL   J Exp Clin Cancer Res 42 (1), 311 (2023)
   PUBMED   37993901
  REMARK    GeneRIF: Liver cancer stem cell dissemination and metastasis:
            uncovering the role of NRCAM in hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7572)
  AUTHORS   Puelles,L. and Hidalgo-Sanchez,M.
  TITLE     The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic
            Organizer
  JOURNAL   Int J Mol Sci 24 (11), 9769 (2023)
   PUBMED   37298722
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7572)
  AUTHORS   Matise,M.P., Lustig,M., Sakurai,T., Grumet,M. and Joyner,A.L.
  TITLE     Ventral midline cells are required for the local control of
            commissural axon guidance in the mouse spinal cord
  JOURNAL   Development 126 (16), 3649-3659 (1999)
   PUBMED   10409510
REFERENCE   7  (bases 1 to 7572)
  AUTHORS   Yamazaki,K., Mizui,Y., Sagane,K. and Tanaka,I.
  TITLE     Genetic mapping of mouse tumor necrosis factor-alpha converting
            enzyme (TACE) to chromosome 12
  JOURNAL   Genomics 49 (2), 336-337 (1998)
   PUBMED   9598327
REFERENCE   8  (bases 1 to 7572)
  AUTHORS   Lane,R.P., Chen,X.N., Yamakawa,K., Vielmetter,J., Korenberg,J.R.
            and Dreyer,W.J.
  TITLE     Characterization of a highly conserved human homolog to the chicken
            neural cell surface protein Bravo/Nr-CAM that maps to chromosome
            band 7q31
  JOURNAL   Genomics 35 (3), 456-465 (1996)
   PUBMED   8812479
REFERENCE   9  (bases 1 to 7572)
  AUTHORS   Burmeister,M., Ren,Q., Makris,G.J., Samson,D. and Bennett,V.
  TITLE     Genes for the neuronal immunoglobulin domain cell adhesion
            molecules neurofascin and Nr-CAM map to mouse chromosomes 1 and 12
            and homologous human chromosomes
  JOURNAL   Mamm Genome 7 (7), 558-559 (1996)
   PUBMED   8672144
REFERENCE   10 (bases 1 to 7572)
  AUTHORS   Moscoso,L.M. and Sanes,J.R.
  TITLE     Expression of four immunoglobulin superfamily adhesion molecules
            (L1, Nr-CAM/Bravo, neurofascin/ABGP, and N-CAM) in the developing
            mouse spinal cord
  JOURNAL   J Comp Neurol 352 (3), 321-334 (1995)
   PUBMED   7706555
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB641673.1, AK122252.1, BC055053.1, AY528242.1 and AC156563.1.
            
            On Mar 25, 2009 this sequence version replaced NM_176930.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ543321.1, SRR9219381.185024.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-269               BB641673.1         1-269
            270-2427            AK122252.1         200-2357
            2428-3711           BC055053.1         2043-3326
            3712-4294           AY528242.1         505-1087
            4295-6818           AC156563.1         45172-47695
            6819-7572           AY528242.1         3615-4368
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 20.71 cM"
     gene            1..7572
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /note="neuronal cell adhesion molecule"
                     /db_xref="GeneID:319504"
                     /db_xref="MGI:MGI:104750"
     exon            1..212
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            213..368
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            369..446
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            447..648
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     CDS             543..4313
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; mBravo; nr-CAM; ng-CAM-related; neuronal
                     surface protein Bravo; ngCAM-related cell adhesion
                     molecule; neuron-glia-CAM-related cell adhesion molecule"
                     /codon_start=1
                     /product="neuronal cell adhesion molecule isoform 1
                     precursor"
                     /protein_id="NP_795904.3"
                     /db_xref="CCDS:CCDS49056.1"
                     /db_xref="GeneID:319504"
                     /db_xref="MGI:MGI:104750"
                     /translation="MQLKIMPKKKHLSAGGVPLILFLCQMISALDVPLDLVQPPTITQ
                     QSPKDYIIDPRENIVIQCEAKGKPPPSFSWTRNGTHFDIDKDPLVTMKPGSGTLVINI
                     MSEGKAETYEGVYQCTARNERGAAVSNNIVVRPSRSPLWTKERLEPIVLQNGQSLVLP
                     CRPPIGLPPAIIFWMDNSFQRLPQSERVSQGLNGDLYFSNVLPEDTREDYICYARFNH
                     TQTIQQKQPISLKVISVDELNDTIAANLSDTEFYGAKSSKERPPTFLTPEGNESHKEE
                     LRGNVLSLECIAEGLPTPIIYWIKEDGMLPANRTFYRNFKKTLQITHVSEADSGNYQC
                     IAKNALGAVHHTISVTVKAAPYWIVAPQNLVLSPGENGTLICRANGNPKPRISWLTNG
                     VPIEIALDDPSRKIDGDTIIFSNVQESSSAVYQCNASNKYGYLLANAFVNVLAEPPRI
                     LTSANTLYQVIANRPALLDCAFFGSPMPTIEWFKGTKGSALHEDIYVLHDNGTLEIPV
                     AQKDSTGTYTCVARNKLGMAKNEVHLEIKDPTRIIKQPEYAVVQRGSKVSFECRVKHD
                     HTLIPTIMWLKDNGELPNDERFSTDKDHLVVSDVKDDDGGTYTCTANTTLDSASASAV
                     LRVVAPTPTPAPIYDVPNPPFDLELTNQLDKSVQLTWTPGDDNNSPITKFIIEYEDAM
                     HDAGLWRHQAEVSGTQTTAQLKLSPYVNYSFRVMAENSIGRSMPSEASEQYLTKAAEP
                     DQNPMAVEGLGTEPDNLVITWKPLNGFQSNGPGLQYKVSWRQKDGDDEWTSVVVANVS
                     KYIVSGTPTFVPYLIKVQALNDVGFAPEPAAVMGHSGEDLPMVAPGNVRVSVVNSTLA
                     EVHWDPVPPKSVRGHLQGYRIYYWKTQSSSKRNRRHIEKKILTFQGTKTHGMLPGLQP
                     YSHYALNVRVVNGKGEGPASTDRGFHTPEGVPSAPSSLKIVNPTLDSLTLEWDPPSHP
                     NGILTEYILQYQPINSTHELGPLVDLKIPANKTRWTLKNLNFSTRYKFYFYAQTSVGP
                     GSQITEEAITTVDEAGIPPPDVGAGKGKEEWRKEIVNGSRSFFGLKGLMPGTAYKVRV
                     GAEGDSGFVSSEDVFETGPAMASRQVDIATQGWFIGLMCAVALLILILLIVCFIRRNK
                     GGKYPVKEKEDAHADPEIQPMKEDDGTFGEYSDAEDHKPLKKGSRTPSDRTVKKEDSD
                     DSLVDYGEGVNGQFNEDGSFIGQYSGKKEKEPAEGNESSEAPSPVNAMNSFV"
     exon            649..754
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            755..951
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1074
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1245
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1302
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1414
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1599
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1731
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1875
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..1987
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2154
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            2155..2302
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..2427
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2457
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            2458..2559
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            2560..2757
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            2758..2828
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            2829..3054
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            3055..3170
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            3171..3375
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            3376..3498
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            3499..3675
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            3676..3711
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            3712..3864
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..3996
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            3997..4075
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     exon            4076..7572
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4546..4551
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /note="hexamer: ATTAAA"
     polyA_site      4557
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /note="major polyA site"
     polyA_site      4567
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
     polyA_site      4729
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
     regulatory      5742..5747
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /note="hexamer: AATAAA"
     polyA_site      5761
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
     regulatory      6414..6419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /note="hexamer: ATTAAA"
     regulatory      6420..6425
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
                     /note="hexamer: AATGAA"
     polyA_site      6432
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
     polyA_site      6458
                     /gene="Nrcam"
                     /gene_synonym="Bravo; C030017F07Rik; C130076O07Rik;
                     mKIAA0343"
ORIGIN      
        1 ggagcggcga ggacgcgcgc ttcgcctggg tcgctggcgg acgcgcgccg gagtccgagc
       61 ctgagacgcg cgggcgcgag ggaccgtgca gaaacggaga ctggggcagc tggaggcgac
      121 agcttcggaa gcttcggaaa cacgcacgac agctgggctc cggggtctta ggcagtagcg
      181 ccccggcagg gaggtgggcg cggcacgaaa acgaattttt gtgctgctct ccagtgaaag
      241 ccagtacccc tatttgctga aggcttttta accaaggctg ctttcagctc ttgacattac
      301 ccagagaaaa ggtggactca gaaatcaggg aagcattgga ggtttctgac cctgaggatc
      361 ttctgaagag caatgctaag aaaacctttg gcttaaggaa ttaaaacgaa ataccacttc
      421 ttcaagtaag aagacctact tcaaagtccc ctgcatgaaa aattatttaa acgccgcaca
      481 ccaatcttcc gtgagagaag aaaaggaaat tccgtgcgtg ggtctcagca ggagtgcagc
      541 tgatgcagct taaaataatg ccgaagaaga agcacttatc tgcaggcgga gtgcccctga
      601 ttctcttcct gtgccagatg atcagcgcgc tggatgttcc tctcgatctg gtacaacctc
      661 caaccatcac tcaacagtca ccaaaagact acatcattga ccctcgggag aatattgtaa
      721 tccagtgtga ggccaaaggg aaacctcccc caagcttttc ctggactcgt aatggaactc
      781 actttgacat agacaaggac cctctggtca ccatgaagcc tggctcagga acccttgtca
      841 tcaacatcat gagtgagggg aaggcagaga cctatgaagg agtctaccag tgcactgcaa
      901 ggaacgagcg tggagctgcc gtctccaata acattgttgt ccgcccatct aggtcaccct
      961 tgtggaccaa ggaaagactt gaacctatag tactccagaa tgggcagtca ttagtacttc
     1021 catgtaggcc tccgattgga ttacctccgg ccataatatt ttggatggat aattcctttc
     1081 agagacttcc acagagtgag cgggtttccc aaggcctaaa tggagacctt tacttctcca
     1141 atgtcctccc agaggacacc cgtgaggact atatctgcta tgccagattt aatcacactc
     1201 aaaccatcca gcagaagcag cctatttctt tgaaggtgat ttcagtggat gaattgaatg
     1261 acactatagc tgctaatttg agtgacactg agttttatgg tgctaaatct agtaaagaga
     1321 ggcccccaac gtttctaact ccagagggca atgaaagcca caaagaggaa ttaagaggaa
     1381 acgtgctttc gctggagtgc attgcggaag gcctacctac tccaattatt tactggatca
     1441 aagaagacgg aatgcttccc gccaaccgga cattttatcg gaactttaag aaaaccctgc
     1501 agatcactca tgtttccgaa gccgactctg gaaattatca gtgcatagca aaaaacgcat
     1561 taggagccgt ccatcacacc atttctgtca ctgttaaagc ggctccctac tggatcgtgg
     1621 cacctcaaaa cctcgtgctt tccccgggag agaatgggac cctcatctgc agagctaatg
     1681 gcaacccgaa acccagaatt agctggttaa caaatggagt cccaatagaa attgctctcg
     1741 atgaccccag cagaaaaata gatggcgata ccattatatt ttcaaatgtt caagaaagct
     1801 caagtgcggt ttatcagtgc aatgcctcta acaaatatgg atatttacta gcaaatgcat
     1861 ttgtaaatgt tctcgctgaa ccacctcgga ttctcacatc agcaaacaca ctgtaccagg
     1921 tcattgcaaa caggcctgct ttgctagatt gtgccttctt tggatctcct atgcctacca
     1981 ttgagtggtt taaaggcact aaaggaagcg ctcttcatga agacatttat gttttgcatg
     2041 ataatggaac attagaaatt cccgtggccc aaaaggatag tacagggacg tatacttgtg
     2101 tcgcacggaa taaactaggg atggcaaaga atgaagttca cttggaaatc aaagatccaa
     2161 ccaggatcat taaacaacct gagtatgcag tcgtccagag ggggagcaag gtgtcctttg
     2221 aatgcagagt gaaacatgac cacaccttaa tccccaccat tatgtggctg aaggacaatg
     2281 gagagctgcc caatgatgaa aggttctcca ctgacaagga tcatctggtg gtatctgatg
     2341 taaaggatga cgatggcgga acctacacgt gtacggccaa cacaacgctg gacagtgctt
     2401 cggccagcgc tgtgctcagg gttgtcgctc ctactccaac tccagccccc atttacgatg
     2461 tcccgaatcc tccctttgat ttagaattga ccaatcaact tgacaaaagt gttcagctga
     2521 catggacccc aggcgacgac aacaatagcc ccattacaaa attcatcatt gagtatgaag
     2581 atgcaatgca tgatgcaggg ctgtggcgcc accaggctga agtttctgga acacagacca
     2641 cagcccaact gaagctgtct ccctatgtga actactcctt ccgtgtcatg gcagagaaca
     2701 gcattgggag aagtatgccg agcgaggcat ccgagcagta tcttacaaaa gccgcagaac
     2761 cagatcagaa tcccatggct gtggaaggac tagggacaga gccggacaac ttggtgatta
     2821 catggaagcc cctgaatggt tttcaatcga atgggcctgg cctccagtac aaagtgagct
     2881 ggcgccagaa agatggtgac gatgagtgga cgtctgtggt tgtggccaat gtatccaaat
     2941 acattgtttc tggcacacca acctttgtcc catacctgat aaaagttcaa gctctgaatg
     3001 atgtggggtt tgcaccagag ccagctgcag tcatggggca ttctggagaa gaccttccaa
     3061 tggtggctcc tggaaatgtt cgcgtcagcg tggtgaacag tacgctggca gaggtgcact
     3121 gggacccagt tcctcccaag agtgtccgag gacacttaca aggctaccgg atttactact
     3181 ggaagaccca gagctcctct aaaagaaaca ggcgccacat tgagaagaag atcctcacct
     3241 tccagggcac caagactcac ggcatgctgc cagggctgca gccatacagt cactatgccc
     3301 tcaacgtccg agtggtcaac gggaaagggg agggcccagc cagcacggac agaggcttcc
     3361 atacaccgga gggagtccct agtgctccct catctttgaa aattgttaat cccacactgg
     3421 actccctcac tttggaatgg gaccccccaa gccacccaaa tggcatcctg actgagtaca
     3481 tcttacaata tcagccaatt aacagcacac atgagctagg ccctctggta gatttaaaaa
     3541 ttcctgccaa caagacccgc tggactttga aaaatttaaa tttcagcact cggtacaagt
     3601 tctatttcta tgcacagaca tcagtggggc caggcagtca gatcacagag gaagcgataa
     3661 cgactgtgga cgaagctggt attcctccac ctgatgtagg tgcaggcaaa ggcaaagaag
     3721 aatggaggaa agaaattgta aatggttctc gaagcttctt tgggttaaag ggtctaatgc
     3781 caggaacagc atacaaagtt cgagttggtg ctgaggggga ctctggtttt gtgagttcag
     3841 aggatgtgtt tgagacagga ccagcaatgg caagccggca agtggatatc gcgacccaag
     3901 gctggttcat aggtctaatg tgcgctgttg ctctcctcat cttaattttg ctgattgttt
     3961 gcttcatcag aagaaacaaa ggtggtaaat acccagttaa agaaaaggag gatgctcatg
     4021 cagaccctga aatccagccc atgaaggaag atgatgggac gtttggagaa tacagtgatg
     4081 cagaagatca caagcctttg aaaaaaggaa gtcgaacacc ttcagacagg actgtgaaaa
     4141 aagaagatag cgatgatagt ctggttgact atggagaggg ggtgaacggc caattcaacg
     4201 aggatggctc ctttattggc caatacagtg gtaagaaaga gaaagagcca gcagagggaa
     4261 atgaaagctc agaggcccct tctcctgtca acgcaatgaa ctcctttgtt taagtcttta
     4321 agctctgtgc caatgtctaa tctctctgga atggttttcc tgaacacctg tcatccctct
     4381 catactacgg acatatgggt agaaagaatg ttttctgcgg tatatgagta ttatgagacc
     4441 agagcaagaa catgactcag tcaaatgata cattaatatg aactggggtg caaagtgcat
     4501 acgtttttgt tcagagtggg gtttttctca atttccttgg aactgattaa aaagaaatac
     4561 aacatcatca atagattaca ttattccctg tgcgggatcc agttcaatac gatgcatgag
     4621 acatgctgca cagaggacac acctgtgtat gtgactgcga tgtggttggg tactttgttg
     4681 gcactatcct cagctgatcc ctaaatatga attccgttgt cattgtgaag agtgttgctg
     4741 tagtgttctc tagagcttca atgtctctgt ggacattgtt gttaaactcg tgactatgtc
     4801 tagctggtgt tagtcttttt gagagactat taggaacagt gtgtacagta tatacttgct
     4861 acatgagtta cttatgacag ttgcatccac taatgctcgc tgagactctg aaacctgttc
     4921 tttgctcccg gaccttccta ggctttttat ccttccgggt gtcacagcag cagagtccca
     4981 cttactatac tatctgcgtt cagtcctttg gggggcataa acattgcgta ttgtgacacc
     5041 tgctggcatt tgtgccctac tcaaagaatt gaagtcaaag ctctcaagtg aaattttaaa
     5101 ttttagttca gaaaatgcag ggcaagacac acgcagtgcc ttcagacaat tagcattccc
     5161 tgactcctgt actctgtatc agccagtgtg ggcaacttca tcctacgcta ctgttgttgg
     5221 aaaggaactt ttccaaggca gagataattc gaactgaaac tgggagcata ggaacaactt
     5281 tatttgaagt ggtgttcccc acacaaatat caggcctcca aagctctgtc attgtcgggt
     5341 gtacaggatg gttttgttgt cttttgtggt ttctagccaa acaagtcaag ttgaaaaaag
     5401 gaaaaaatgg tatggttgga aatggataga aaacccaatg attgcagaag taaaagcgta
     5461 caacagcatg cctaaagtac taaatgattc actctgccta gacaagcttt caaattccgc
     5521 agcattcagc aacgtgtatg caaattgcaa caggcacact ccagaacctg agaaacctga
     5581 ccagtgctta attaggccag cagtcaaaga gattacacct cgagtctaaa gaacaatact
     5641 atatcctatc atagccataa acaatgtgtg tgaaatggct tacctccaat atagaaacct
     5701 atgaccacct atggttgaag ggatgctcag tttcatttgc caataaattg gtttctcata
     5761 acttgcatca agtttaattt taagtaaagc cttttatatg taggtattct gttgaatttg
     5821 taaatatact tagagtgtag aagctatatg ctcataggta ttacgtgcaa ataaacatgc
     5881 aatcaatgtt tatgttctcc tgtgtgagaa gcaggctagt tgatgaatgt agtaagtatg
     5941 tgggacgagg aaacatccaa atgtctttcg tggtgtttcg gatttaattt ttttccccac
     6001 tctatttttg tttgtgaaac tgaaatagtg aactccgtgc cccccacccc tttcttcagc
     6061 ctgttgctgg ctgaaagcct cagtgttttg ccaatggagt acattagggt ttgccacctg
     6121 acacctttga tgtgtacagg taaagcttgt tttaaagtga ttccccaaat gaagtggccc
     6181 taaatactct catcttttca tattcactct ccacaaacta gcctatcgat tgaaaataca
     6241 aaataacaca caggcttgcc tcaacataac aaagcacaaa gacaaaagtt cctttcaagt
     6301 catcaatgcc ttcagtgaaa gacaaatgtg aagtctctgc tcatctataa ctatggatgc
     6361 taaatttctc tgtgcatgaa gaagtcacgc ctgtagataa ctacagattt tctattaaaa
     6421 atgaaaaatt gagtgtggtt ttaaagcccc cccccccact ccttgtactt agtcatgttc
     6481 ctgggggggg gggtgggggg agggtgggtg tgtagcaggt gtacccggca gcacaatgct
     6541 tctggacttc catctgaaaa gggactggta aacagcccag ctctcctcag tttcagcttc
     6601 ttcaaacctg tacttccaaa gatttttgaa caaattctga caattttgtt tcgtttttaa
     6661 aagactttgg attttatcgc caacgtaaaa ataagaatta gaatcaggaa ttattttttt
     6721 ttttccgaga cagggtttct ctgtgtagtc ttggctgtcc tggaactcat tctgcagacc
     6781 aggctggcct ggaactcaga aatctgcctg cctctgcctc ccaagtgctg ggaaaggcgt
     6841 gagccaccac cgcccggtga aatcaggaac ttttaaatca aaggttaggg ctagttacgc
     6901 aggaatagat agcaagagtc ctttgaaaac aggtgaacaa atctggttcc tttgtcgagt
     6961 ctcacaatag accaactgct ggtgccctgt ccatacaacc agtgggacct cctcgctact
     7021 cagccatggt caatttatat ttcttaacat ggctacatga tgagagtttg aacactcata
     7081 catattacaa atatgtcatc tgcttccttt tctccacctt cattcttctg tctccatcca
     7141 agcctagcaa ttatccacat tccaccactt gccccttggt cactgacccc tggtcccagc
     7201 agtgcatcca ggagaactac ctgccttgtg ctctcagagt tatttagaca gcatgctatg
     7261 aaagtgaatc ggagatgcca tagagagaga gctcagaatt cctctcacag aggacatgtt
     7321 ggctgctttc tcggaccctc ccttctgtcc tcactgtggg ccaaattaac aaaccttagc
     7381 ctcactctac atcatgacac ggctccgtgg caacaggaac tttgaaactt taagcatgac
     7441 ctcagaaaaa atcttgtagt tataaacctt tataaaccat cttcttcatt gggttttaag
     7501 gactttcgag gtaaggggag tctaagaact cagggatttt cagtcacaaa atgagtcact
     7561 tccgcttgag at
//
