LOCUS       NM_001287102            4712 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus nephronectin (Npnt), transcript variant 4, mRNA.
ACCESSION   NM_001287102
VERSION     NM_001287102.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Garis,M., Meyer,M.D. and Lwigale,P.
  TITLE     Expression of Nephronectin in the Descemet's membrane of mouse
            corneas during development and adult homeostasis
  JOURNAL   Exp Eye Res 240, 109797 (2024)
   PUBMED   38246333
  REMARK    GeneRIF: Expression of Nephronectin in the Descemet's membrane of
            mouse corneas during development and adult homeostasis.
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Wilson,C.L., Hung,C.F., Burkel,B.M., Ponik,S.M., Gharib,S.A. and
            Schnapp,L.M.
  TITLE     Nephronectin is required to maintain right lung lobar separation
            during embryonic development
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 324 (3), L335-L344 (2023)
   PUBMED   36719987
  REMARK    GeneRIF: Nephronectin is required to maintain right lung lobar
            separation during embryonic development.
REFERENCE   3  (bases 1 to 4712)
  AUTHORS   Wilson,C.L., Hung,C.F. and Schnapp,L.M.
  TITLE     Endotoxin-induced acute lung injury in mice with postnatal deletion
            of nephronectin
  JOURNAL   PLoS One 17 (5), e0268398 (2022)
   PUBMED   35552565
  REMARK    GeneRIF: Endotoxin-induced acute lung injury in mice with postnatal
            deletion of nephronectin.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4712)
  AUTHORS   Maimets,M., Pedersen,M.T., Guiu,J., Dreier,J., Thodberg,M.,
            Antoku,Y., Schweiger,P.J., Rib,L., Bressan,R.B., Miao,Y.,
            Garcia,K.C., Sandelin,A., Serup,P. and Jensen,K.B.
  TITLE     Mesenchymal-epithelial crosstalk shapes intestinal regionalisation
            via Wnt and Shh signalling
  JOURNAL   Nat Commun 13 (1), 715 (2022)
   PUBMED   35132078
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4712)
  AUTHORS   Linton,J.M., Martin,G.R. and Reichardt,L.F.
  TITLE     The ECM protein nephronectin promotes kidney development via
            integrin alpha8beta1-mediated stimulation of Gdnf expression
  JOURNAL   Development 134 (13), 2501-2509 (2007)
   PUBMED   17537792
  REMARK    GeneRIF: results thus place nephronectin and alpha8beta1 integrin
            in a pathway that regulates Gdnf expression and is essential for
            kidney development
REFERENCE   6  (bases 1 to 4712)
  AUTHORS   Hecht,J., Seitz,V., Urban,M., Wagner,F., Robinson,P.N., Stiege,A.,
            Dieterich,C., Kornak,U., Wilkening,U., Brieske,N., Zwingman,C.,
            Kidess,A., Stricker,S. and Mundlos,S.
  TITLE     Detection of novel skeletogenesis target genes by comprehensive
            analysis of a Runx2(-/-) mouse model
  JOURNAL   Gene Expr Patterns 7 (1-2), 102-112 (2007)
   PUBMED   16829211
REFERENCE   7  (bases 1 to 4712)
  AUTHORS   Kikkawa,Y., Virtanen,I. and Miner,J.H.
  TITLE     Mesangial cells organize the glomerular capillaries by adhering to
            the G domain of laminin alpha5 in the glomerular basement membrane
  JOURNAL   J Cell Biol 161 (1), 187-196 (2003)
   PUBMED   12682087
REFERENCE   8  (bases 1 to 4712)
  AUTHORS   Morimura,N., Tezuka,Y., Watanabe,N., Yasuda,M., Miyatani,S.,
            Hozumi,N. and Tezuka Ki,K.
  TITLE     Molecular cloning of POEM: a novel adhesion molecule that interacts
            with alpha8beta1 integrin
  JOURNAL   J Biol Chem 276 (45), 42172-42181 (2001)
   PUBMED   11546798
REFERENCE   9  (bases 1 to 4712)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 4712)
  AUTHORS   Brandenberger,R., Schmidt,A., Linton,J., Wang,D., Backus,C.,
            Denda,S., Muller,U. and Reichardt,L.F.
  TITLE     Identification and characterization of a novel extracellular matrix
            protein nephronectin that is associated with integrin alpha8beta1
            in the embryonic kidney
  JOURNAL   J Cell Biol 154 (2), 447-458 (2001)
   PUBMED   11470831
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB059656.1, AF397007.1, AK160311.1 and AC139942.4.
            
            Transcript Variant: This variant (4) lacks an in-frame exon in the
            5' coding region compared to variant 3. The encoded isoform (d) is
            shorter than isoform c.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF397007.1, SRR13948564.2005199.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-248               AB059656.1         1-248
            249-2027            AF397007.1         1-1779
            2028-3774           AB059656.1         1935-3681
            3775-4125           AK160311.1         239-589
            4126-4249           AC139942.4         81735-81858
            4250-4710           AK160311.1         714-1174
            4711-4712           AC139942.4         82320-82321
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 61.52 cM"
     gene            1..4712
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /note="nephronectin"
                     /db_xref="GeneID:114249"
                     /db_xref="MGI:MGI:2148811"
     exon            1..319
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..2027
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /note="isoform d precursor is encoded by transcript
                     variant 4; preosteoblast EGF-like repeat protein with MAM
                     domain"
                     /codon_start=1
                     /product="nephronectin isoform d precursor"
                     /protein_id="NP_001274031.1"
                     /db_xref="CCDS:CCDS71322.1"
                     /db_xref="GeneID:114249"
                     /db_xref="MGI:MGI:2148811"
                     /translation="MAVLLAAVLASSLYLQVAADFDGRWPRQIVSSIGLCRYGGRIDC
                     CWGWARQSWGQCQPVCQPQCKHGECVGPNKCKCHPGFAGKTCNQDESFHPTPLDQGSE
                     QPLFQPPDHQATNVPSRDLNECGLKPRPCKHRCMNTFGSYKCYCLNGYMLLPDGSCSS
                     ALSCSMANCQYGCDVVKGQVRCQCPSPGLQLAPDGRTCVDIDECATGRVSCPRFRQCV
                     NTFGSYICKCHTGFDLMYIGGKYQCHDIDECSLGQHQCSSYARCYNIHGSYKCQCRDG
                     YEGDGLNCVYIPKVMIEPSGPIHMPERNGTISKGDGGHANRIPDAGSTRWPLKTPYIP
                     PVITNRPTSKPTTRPTPNPTPQPTPPPPPPLPTEPRTTPLPPTPERPSTRPTTIAPAT
                     STTTRVITVDNRIQTDPQKPRGDVFIPRQPTNDLFEIFEIERGVSADEEVKDDPGILI
                     HSCNFDHGLCGWIREKDSDLHWETARDPAGGQYLTVSAAKAPGGKAARLVLRLGHLMH
                     SGDLCLSFRHKVTGLHSGTLQVFVRKHGTHGAALWGRNGGHGWRQTQITLRGADVKSV
                     IFKGEKRRGHTGEIGLDDVSLKRGRC"
     exon            320..420
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            421..513
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            514..606
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            607..726
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            727..846
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            847..981
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1104
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1500
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1587
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1686
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1943
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..4712
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4687..4692
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /note="hexamer: AATAAA"
     polyA_site      4712
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /note="major polyA site"
ORIGIN      
        1 gcggcgggct gcgcggcgcg tccctcccgc ctcgcccctc cccgcgggcg gcgcggctcc
       61 cgccgcagcg cctcccctcg ccaggaggga gccggagggg ctctgccagc ccgccgcaga
      121 cctgcccggc tgcgcgctgc gctctgtgcg tcccagcagc cgcgggtgcc gcgccgaagg
      181 cggccgggag atcccgggac gcgcagctcc ccgcaacctt cagcgtcccc gcgtcccggt
      241 cctccggcat ggctgtgctc ctagcggcgg tgctcgcgtc ctcgctctac ctgcaggtgg
      301 ctgccgactt cgacgggagg tggcccaggc aaatagtttc ttcaatcggc ctatgtcgct
      361 atggaggcag gattgactgc tgctggggct gggcccgcca gtcctgggga cagtgccaac
      421 ctgtgtgtca accacagtgc aaacacggag agtgcgtcgg gccaaacaag tgcaaatgtc
      481 accctggatt tgctgggaaa acctgcaacc aagatgagtc cttccaccca actcctcttg
      541 accaaggcag tgaacagcct cttttccaac ccccggatca tcaagccaca aatgtacctt
      601 caagggattt aaacgagtgt ggcctgaagc ctcggccctg taagcacagg tgcatgaaca
      661 ctttcggcag ctacaagtgc tactgtctca acggatacat gctgctgcca gacgggtcct
      721 gctcaagtgc cctatcgtgt tccatggcaa actgtcagta tggctgtgac gtggtcaaag
      781 gacaggtccg atgtcagtgt ccatcccctg gcctgcagct agctcccgat gggaggacct
      841 gtgtggatat tgatgaatgt gcgactggaa gagtctcctg ccctcgattt aggcagtgtg
      901 tcaacacgtt tgggagctac atctgcaagt gtcacactgg tttcgacctc atgtacattg
      961 gaggcaaata tcagtgccat gacatcgacg agtgctctct tggacagcac cagtgtagca
     1021 gctatgcccg gtgttacaac atacatgggt cctacaagtg ccaatgtaga gatggatacg
     1081 agggggatgg actgaactgt gtgtatatcc ccaaagtcat gattgaacct tcaggtccaa
     1141 tccatatgcc agaaagaaat ggtacaatct caaagggtga tggaggacat gcgaatagga
     1201 ttcctgatgc tggaagtaca aggtggcccc tgaagacacc atatattcct cctgtcatta
     1261 ccaacaggcc tacttccaag ccaacaacaa gacctacacc aaacccaaca ccacagccta
     1321 ctccaccacc tccaccaccc ctcccgacag agcccagaac aactccacta ccaccaaccc
     1381 cagaaaggcc atctaccaga cccaccacta tagcacctgc taccagtacc actacacgag
     1441 taattacggt tgacaacagg atacagacgg atcctcagaa acccagagga gatgtgttca
     1501 ttccacggca gccgacaaat gacctgtttg agatatttga aatcgaaaga ggggtcagcg
     1561 cggatgagga agtaaaggac gacccaggta ttctcataca cagctgcaat tttgaccatg
     1621 gactctgtgg atggatcaga gaaaaagata gtgacttgca ctgggagaca gccagggacc
     1681 cagcaggtgg acagtatctc acagtgtctg cagccaaagc cccgggggga aaagccgctc
     1741 gcttggtgtt acgtctcggc cacctcatgc attcagggga cctgtgcctg tcctttaggc
     1801 acaaggtgac tgggctgcac tcgggcacac tgcaggtgtt tgtgaggaaa cacggtaccc
     1861 acggagcagc cctgtgggga agaaatggtg gccatggctg gaggcaaacc cagatcacct
     1921 tgcgaggggc tgacgtcaaa agcgtcatct tcaaaggtga aaaaaggcgt ggtcacacgg
     1981 gggagattgg attggatgat gtgagcttga aaagaggtcg ctgctgagaa gacccctggc
     2041 agctcccgag ctagcagtga atttgtcgct ctccctcatt tcccaatgct tgccctcttg
     2101 tctccctctt atcaggccta gggcaggagt gggtcaggag gaaggttgct tggtgactcg
     2161 ggtctcggtg gcctgttttg gtgcaatccc agtgaacagt gacactctcg aagtacagga
     2221 gcatctggag acacctccgg gcccttctgg ggtgttacct tatatggtgt cttctttagg
     2281 aaggcctttg gcatgtgtga ccgggaccat ccttcatcct ggttatagag tgggcctgct
     2341 agccaactac gggagacgtt ttcattctgt gcaaatggta ttctgtgatc tgtccagtgt
     2401 tgtaccatga gtagtactga ctttgcttac agtatgacag tatgatgtac agcgtgcttg
     2461 tgaaactagt gtatcttttt cacttcagtt acttctggcc tgacctcaat ccatattctt
     2521 tataacggag gggtgtgcaa catattaaga tgcatttatt cttgtaaact gtgtctttct
     2581 gttaaagaca gttatatagg gtgggcatgg aatttcctgg ttagcagtac tgtgtttgtg
     2641 taaatgtgct attactataa gtatttacat gttccgaata tccacagact ctagttgcaa
     2701 ggtcaaaggc agcttatgat cccctgagtt aaaaaaaaaa tacatggtga cctgtcatct
     2761 atgctatgac ttaaaccagc agcaagaaaa ctggcaaagg tgtgcggctt ggggtagtgt
     2821 catgatggct ttgtttctgt gtccttgagt ttgacctatt cagaaaacca ttcaagggaa
     2881 gcaaggacct gcagttaata tataaaaccc ctatgcttcc ggttatactt taaactgaca
     2941 gccttcacca ctgttacatg cctccaccag taagaagctt tgtaaagatt ctgggggggg
     3001 gagcggagga ccagaggaca gcccataaga caggtgcttt aaatcagcaa ggtttccacg
     3061 agtgcagcta atgtgttacc tgaggctggg ctaggaagca ggcctgtgca cagaggtgac
     3121 gctgccgatg atggagcaac actagcactg tcgaggagga caagattgga attttaatat
     3181 cgacagagcc ccaacttcgt ctcctccctg tcagaccgct gttttaaaaa aacacatagc
     3241 tttagagagt caacttttct ccttagcatt tctcaccctt ctgattaagt aatcaaaata
     3301 ttttctgctg tttttgccag gagacacaaa gatgattaaa gggttggaaa aaaagatcta
     3361 tggtgacaaa ctaaaggaac tgggagtgtt ctgcctggag aagagaagac tgaggggcag
     3421 gccgtggggg ctcctccagg ctgcgaaggg ttggcaccaa gagcttgggg agcggctgtt
     3481 ctccatgtgt cctgagaata ggaccagagg gaacaggctt aggctagagt gtgaggggga
     3541 ctttcctggc agggacagtt gctaagccca atcctttaca aaaaagaagt gtgatcatat
     3601 tcattctcct cctactccct ccttccctcc ctcccttcct tccttctttc ctccctccct
     3661 tccttctttc ctccccccct tacttctacc ttcatctctc tctctccctt tctgttctct
     3721 tctcttcctc tctatcttct ttgtcccttc cctccattaa aaaaaaaaaa aaaactacat
     3781 aaaatgttag gtatttaagg tgtacgtact tcaaggtaaa gtccctgatc atggacacta
     3841 gaagacactt tggtctccaa agtagtccat gataatgacc atcttcagaa ctgagcagtt
     3901 tggactgagc tccaaggtag actttctgct cttggcctcc tcctgtaaag ggcacaaaag
     3961 gaaatgtcaa gctcactgaa agggtcttca ctcaacagcc aacccagcag tattaacaga
     4021 acaaatcccc aaattcttta ttcctggaga gggtgtgtca cacaggtgct atcgaatctt
     4081 aataatgtcc taatatggtg gtgttaatgt ttgattcctg ggtaagggtg cgacccccca
     4141 cggtttgttc agtcgatgct tagacttgtt tttcaaagag acccccaaga aacatgaggc
     4201 agagagagcc tgtctggtgc attgcctggc ttgcattgac aagttaacat ggctactgaa
     4261 ggtgtgtcaa ccacagcacc gttagccctc gtccttgcct ccttccatga ctcactttct
     4321 caccaggagc cttccaagtt ccacgagctg gtaatgcttt ttgaacagct ccaagtgact
     4381 ttgccattta gctcaacaga cacagatttg gggcttctga agtagaagta aagagcacct
     4441 aagatgtcct taggtccatt ttttttaatg attcatttcc ttcttggtca tgtaactgca
     4501 cagctgattc agaagaaagg ggaacgtaat cgagaatttc actcccaggt gccaataaga
     4561 cattgcacta cactgatgga ggaagttatc caaagtactg tataacatct tgtttattat
     4621 ttaatgttct ctcaagtgag aattgtttgt ggttttcaaa atgaacaaat aaaagcaatc
     4681 tttgtaaata aaaatcactg ttcctactgc ca
//
