LOCUS       NM_001287101            4763 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus nephronectin (Npnt), transcript variant 3, mRNA.
ACCESSION   NM_001287101
VERSION     NM_001287101.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Garis,M., Meyer,M.D. and Lwigale,P.
  TITLE     Expression of Nephronectin in the Descemet's membrane of mouse
            corneas during development and adult homeostasis
  JOURNAL   Exp Eye Res 240, 109797 (2024)
   PUBMED   38246333
  REMARK    GeneRIF: Expression of Nephronectin in the Descemet's membrane of
            mouse corneas during development and adult homeostasis.
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Wilson,C.L., Hung,C.F., Burkel,B.M., Ponik,S.M., Gharib,S.A. and
            Schnapp,L.M.
  TITLE     Nephronectin is required to maintain right lung lobar separation
            during embryonic development
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 324 (3), L335-L344 (2023)
   PUBMED   36719987
  REMARK    GeneRIF: Nephronectin is required to maintain right lung lobar
            separation during embryonic development.
REFERENCE   3  (bases 1 to 4763)
  AUTHORS   Wilson,C.L., Hung,C.F. and Schnapp,L.M.
  TITLE     Endotoxin-induced acute lung injury in mice with postnatal deletion
            of nephronectin
  JOURNAL   PLoS One 17 (5), e0268398 (2022)
   PUBMED   35552565
  REMARK    GeneRIF: Endotoxin-induced acute lung injury in mice with postnatal
            deletion of nephronectin.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4763)
  AUTHORS   Maimets,M., Pedersen,M.T., Guiu,J., Dreier,J., Thodberg,M.,
            Antoku,Y., Schweiger,P.J., Rib,L., Bressan,R.B., Miao,Y.,
            Garcia,K.C., Sandelin,A., Serup,P. and Jensen,K.B.
  TITLE     Mesenchymal-epithelial crosstalk shapes intestinal regionalisation
            via Wnt and Shh signalling
  JOURNAL   Nat Commun 13 (1), 715 (2022)
   PUBMED   35132078
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4763)
  AUTHORS   Linton,J.M., Martin,G.R. and Reichardt,L.F.
  TITLE     The ECM protein nephronectin promotes kidney development via
            integrin alpha8beta1-mediated stimulation of Gdnf expression
  JOURNAL   Development 134 (13), 2501-2509 (2007)
   PUBMED   17537792
  REMARK    GeneRIF: results thus place nephronectin and alpha8beta1 integrin
            in a pathway that regulates Gdnf expression and is essential for
            kidney development
REFERENCE   6  (bases 1 to 4763)
  AUTHORS   Hecht,J., Seitz,V., Urban,M., Wagner,F., Robinson,P.N., Stiege,A.,
            Dieterich,C., Kornak,U., Wilkening,U., Brieske,N., Zwingman,C.,
            Kidess,A., Stricker,S. and Mundlos,S.
  TITLE     Detection of novel skeletogenesis target genes by comprehensive
            analysis of a Runx2(-/-) mouse model
  JOURNAL   Gene Expr Patterns 7 (1-2), 102-112 (2007)
   PUBMED   16829211
REFERENCE   7  (bases 1 to 4763)
  AUTHORS   Kikkawa,Y., Virtanen,I. and Miner,J.H.
  TITLE     Mesangial cells organize the glomerular capillaries by adhering to
            the G domain of laminin alpha5 in the glomerular basement membrane
  JOURNAL   J Cell Biol 161 (1), 187-196 (2003)
   PUBMED   12682087
REFERENCE   8  (bases 1 to 4763)
  AUTHORS   Morimura,N., Tezuka,Y., Watanabe,N., Yasuda,M., Miyatani,S.,
            Hozumi,N. and Tezuka Ki,K.
  TITLE     Molecular cloning of POEM: a novel adhesion molecule that interacts
            with alpha8beta1 integrin
  JOURNAL   J Biol Chem 276 (45), 42172-42181 (2001)
   PUBMED   11546798
REFERENCE   9  (bases 1 to 4763)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 4763)
  AUTHORS   Brandenberger,R., Schmidt,A., Linton,J., Wang,D., Backus,C.,
            Denda,S., Muller,U. and Reichardt,L.F.
  TITLE     Identification and characterization of a novel extracellular matrix
            protein nephronectin that is associated with integrin alpha8beta1
            in the embryonic kidney
  JOURNAL   J Cell Biol 154 (2), 447-458 (2001)
   PUBMED   11470831
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB059656.1, AF397008.1, AK160311.1 and AC139942.4.
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longest isoform (c).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF397008.1, SRR13948564.3162532.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-248               AB059656.1         1-248
            249-2078            AF397008.1         1-1830
            2079-3825           AB059656.1         1935-3681
            3826-4176           AK160311.1         239-589
            4177-4300           AC139942.4         81735-81858
            4301-4761           AK160311.1         714-1174
            4762-4763           AC139942.4         82320-82321
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 61.52 cM"
     gene            1..4763
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /note="nephronectin"
                     /db_xref="GeneID:114249"
                     /db_xref="MGI:MGI:2148811"
     exon            1..319
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..2078
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /note="isoform c precursor is encoded by transcript
                     variant 3; preosteoblast EGF-like repeat protein with MAM
                     domain"
                     /codon_start=1
                     /product="nephronectin isoform c precursor"
                     /protein_id="NP_001274030.1"
                     /db_xref="CCDS:CCDS71323.1"
                     /db_xref="GeneID:114249"
                     /db_xref="MGI:MGI:2148811"
                     /translation="MAVLLAAVLASSLYLQVAADFDGRWPRQIVSSIGLCRYGGRIDC
                     CWGWARQSWGQCQPFYVLRQRLARIRCQLKAVCQPQCKHGECVGPNKCKCHPGFAGKT
                     CNQDESFHPTPLDQGSEQPLFQPPDHQATNVPSRDLNECGLKPRPCKHRCMNTFGSYK
                     CYCLNGYMLLPDGSCSSALSCSMANCQYGCDVVKGQVRCQCPSPGLQLAPDGRTCVDI
                     DECATGRVSCPRFRQCVNTFGSYICKCHTGFDLMYIGGKYQCHDIDECSLGQHQCSSY
                     ARCYNIHGSYKCQCRDGYEGDGLNCVYIPKVMIEPSGPIHMPERNGTISKGDGGHANR
                     IPDAGSTRWPLKTPYIPPVITNRPTSKPTTRPTPNPTPQPTPPPPPPLPTEPRTTPLP
                     PTPERPSTRPTTIAPATSTTTRVITVDNRIQTDPQKPRGDVFIPRQPTNDLFEIFEIE
                     RGVSADEEVKDDPGILIHSCNFDHGLCGWIREKDSDLHWETARDPAGGQYLTVSAAKA
                     PGGKAARLVLRLGHLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGTHGAALWGRNGGH
                     GWRQTQITLRGADVKSVIFKGEKRRGHTGEIGLDDVSLKRGRC"
     exon            320..420
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            421..471
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            472..564
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            565..657
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            658..777
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            778..897
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1032
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1155
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1551
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1638
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1737
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1994
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..4763
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4738..4743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /note="hexamer: AATAAA"
     polyA_site      4763
                     /gene="Npnt"
                     /gene_synonym="1110009H02Rik; Nctn; POEM"
                     /note="major polyA site"
ORIGIN      
        1 gcggcgggct gcgcggcgcg tccctcccgc ctcgcccctc cccgcgggcg gcgcggctcc
       61 cgccgcagcg cctcccctcg ccaggaggga gccggagggg ctctgccagc ccgccgcaga
      121 cctgcccggc tgcgcgctgc gctctgtgcg tcccagcagc cgcgggtgcc gcgccgaagg
      181 cggccgggag atcccgggac gcgcagctcc ccgcaacctt cagcgtcccc gcgtcccggt
      241 cctccggcat ggctgtgctc ctagcggcgg tgctcgcgtc ctcgctctac ctgcaggtgg
      301 ctgccgactt cgacgggagg tggcccaggc aaatagtttc ttcaatcggc ctatgtcgct
      361 atggaggcag gattgactgc tgctggggct gggcccgcca gtcctgggga cagtgccaac
      421 ctttctacgt cttaaggcag agactagcaa ggataaggtg ccagctcaaa gctgtgtgtc
      481 aaccacagtg caaacacgga gagtgcgtcg ggccaaacaa gtgcaaatgt caccctggat
      541 ttgctgggaa aacctgcaac caagatgagt ccttccaccc aactcctctt gaccaaggca
      601 gtgaacagcc tcttttccaa cccccggatc atcaagccac aaatgtacct tcaagggatt
      661 taaacgagtg tggcctgaag cctcggccct gtaagcacag gtgcatgaac actttcggca
      721 gctacaagtg ctactgtctc aacggataca tgctgctgcc agacgggtcc tgctcaagtg
      781 ccctatcgtg ttccatggca aactgtcagt atggctgtga cgtggtcaaa ggacaggtcc
      841 gatgtcagtg tccatcccct ggcctgcagc tagctcccga tgggaggacc tgtgtggata
      901 ttgatgaatg tgcgactgga agagtctcct gccctcgatt taggcagtgt gtcaacacgt
      961 ttgggagcta catctgcaag tgtcacactg gtttcgacct catgtacatt ggaggcaaat
     1021 atcagtgcca tgacatcgac gagtgctctc ttggacagca ccagtgtagc agctatgccc
     1081 ggtgttacaa catacatggg tcctacaagt gccaatgtag agatggatac gagggggatg
     1141 gactgaactg tgtgtatatc cccaaagtca tgattgaacc ttcaggtcca atccatatgc
     1201 cagaaagaaa tggtacaatc tcaaagggtg atggaggaca tgcgaatagg attcctgatg
     1261 ctggaagtac aaggtggccc ctgaagacac catatattcc tcctgtcatt accaacaggc
     1321 ctacttccaa gccaacaaca agacctacac caaacccaac accacagcct actccaccac
     1381 ctccaccacc cctcccgaca gagcccagaa caactccact accaccaacc ccagaaaggc
     1441 catctaccag acccaccact atagcacctg ctaccagtac cactacacga gtaattacgg
     1501 ttgacaacag gatacagacg gatcctcaga aacccagagg agatgtgttc attccacggc
     1561 agccgacaaa tgacctgttt gagatatttg aaatcgaaag aggggtcagc gcggatgagg
     1621 aagtaaagga cgacccaggt attctcatac acagctgcaa ttttgaccat ggactctgtg
     1681 gatggatcag agaaaaagat agtgacttgc actgggagac agccagggac ccagcaggtg
     1741 gacagtatct cacagtgtct gcagccaaag ccccgggggg aaaagccgct cgcttggtgt
     1801 tacgtctcgg ccacctcatg cattcagggg acctgtgcct gtcctttagg cacaaggtga
     1861 ctgggctgca ctcgggcaca ctgcaggtgt ttgtgaggaa acacggtacc cacggagcag
     1921 ccctgtgggg aagaaatggt ggccatggct ggaggcaaac ccagatcacc ttgcgagggg
     1981 ctgacgtcaa aagcgtcatc ttcaaaggtg aaaaaaggcg tggtcacacg ggggagattg
     2041 gattggatga tgtgagcttg aaaagaggtc gctgctgaga agacccctgg cagctcccga
     2101 gctagcagtg aatttgtcgc tctccctcat ttcccaatgc ttgccctctt gtctccctct
     2161 tatcaggcct agggcaggag tgggtcagga ggaaggttgc ttggtgactc gggtctcggt
     2221 ggcctgtttt ggtgcaatcc cagtgaacag tgacactctc gaagtacagg agcatctgga
     2281 gacacctccg ggcccttctg gggtgttacc ttatatggtg tcttctttag gaaggccttt
     2341 ggcatgtgtg accgggacca tccttcatcc tggttataga gtgggcctgc tagccaacta
     2401 cgggagacgt tttcattctg tgcaaatggt attctgtgat ctgtccagtg ttgtaccatg
     2461 agtagtactg actttgctta cagtatgaca gtatgatgta cagcgtgctt gtgaaactag
     2521 tgtatctttt tcacttcagt tacttctggc ctgacctcaa tccatattct ttataacgga
     2581 ggggtgtgca acatattaag atgcatttat tcttgtaaac tgtgtctttc tgttaaagac
     2641 agttatatag ggtgggcatg gaatttcctg gttagcagta ctgtgtttgt gtaaatgtgc
     2701 tattactata agtatttaca tgttccgaat atccacagac tctagttgca aggtcaaagg
     2761 cagcttatga tcccctgagt taaaaaaaaa atacatggtg acctgtcatc tatgctatga
     2821 cttaaaccag cagcaagaaa actggcaaag gtgtgcggct tggggtagtg tcatgatggc
     2881 tttgtttctg tgtccttgag tttgacctat tcagaaaacc attcaaggga agcaaggacc
     2941 tgcagttaat atataaaacc cctatgcttc cggttatact ttaaactgac agccttcacc
     3001 actgttacat gcctccacca gtaagaagct ttgtaaagat tctggggggg ggagcggagg
     3061 accagaggac agcccataag acaggtgctt taaatcagca aggtttccac gagtgcagct
     3121 aatgtgttac ctgaggctgg gctaggaagc aggcctgtgc acagaggtga cgctgccgat
     3181 gatggagcaa cactagcact gtcgaggagg acaagattgg aattttaata tcgacagagc
     3241 cccaacttcg tctcctccct gtcagaccgc tgttttaaaa aaacacatag ctttagagag
     3301 tcaacttttc tccttagcat ttctcaccct tctgattaag taatcaaaat attttctgct
     3361 gtttttgcca ggagacacaa agatgattaa agggttggaa aaaaagatct atggtgacaa
     3421 actaaaggaa ctgggagtgt tctgcctgga gaagagaaga ctgaggggca ggccgtgggg
     3481 gctcctccag gctgcgaagg gttggcacca agagcttggg gagcggctgt tctccatgtg
     3541 tcctgagaat aggaccagag ggaacaggct taggctagag tgtgaggggg actttcctgg
     3601 cagggacagt tgctaagccc aatcctttac aaaaaagaag tgtgatcata ttcattctcc
     3661 tcctactccc tccttccctc cctcccttcc ttccttcttt cctccctccc ttccttcttt
     3721 cctccccccc ttacttctac cttcatctct ctctctccct ttctgttctc ttctcttcct
     3781 ctctatcttc tttgtccctt ccctccatta aaaaaaaaaa aaaaactaca taaaatgtta
     3841 ggtatttaag gtgtacgtac ttcaaggtaa agtccctgat catggacact agaagacact
     3901 ttggtctcca aagtagtcca tgataatgac catcttcaga actgagcagt ttggactgag
     3961 ctccaaggta gactttctgc tcttggcctc ctcctgtaaa gggcacaaaa ggaaatgtca
     4021 agctcactga aagggtcttc actcaacagc caacccagca gtattaacag aacaaatccc
     4081 caaattcttt attcctggag agggtgtgtc acacaggtgc tatcgaatct taataatgtc
     4141 ctaatatggt ggtgttaatg tttgattcct gggtaagggt gcgacccccc acggtttgtt
     4201 cagtcgatgc ttagacttgt ttttcaaaga gacccccaag aaacatgagg cagagagagc
     4261 ctgtctggtg cattgcctgg cttgcattga caagttaaca tggctactga aggtgtgtca
     4321 accacagcac cgttagccct cgtccttgcc tccttccatg actcactttc tcaccaggag
     4381 ccttccaagt tccacgagct ggtaatgctt tttgaacagc tccaagtgac tttgccattt
     4441 agctcaacag acacagattt ggggcttctg aagtagaagt aaagagcacc taagatgtcc
     4501 ttaggtccat tttttttaat gattcatttc cttcttggtc atgtaactgc acagctgatt
     4561 cagaagaaag gggaacgtaa tcgagaattt cactcccagg tgccaataag acattgcact
     4621 acactgatgg aggaagttat ccaaagtact gtataacatc ttgtttatta tttaatgttc
     4681 tctcaagtga gaattgtttg tggttttcaa aatgaacaaa taaaagcaat ctttgtaaat
     4741 aaaaatcact gttcctactg cca
//
