LOCUS       NM_019459               5661 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus nephrosis 1, nephrin (Nphs1), mRNA.
ACCESSION   NM_019459
VERSION     NM_019459.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Siegerist,F., Kliewe,F., Hammer,E., Schakau,P., Chi Soh,J.E.,
            Weber,C., Lindenmeyer,M., Reichelt-Wurm,S., Drenic,V.,
            Chatziantoniou,C., Chadjichristos,C.E., Zhang,Y., Simm,S.,
            Banas,M.C., Nauck,M., Volker,U. and Endlich,N.
  TITLE     The role of the tricellular junction protein ILDR2 in
            glomerulopathies: Expression patterns and functional insights
  JOURNAL   iScience 27 (12), 111329 (2024)
   PUBMED   39640577
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Yano-Sakamoto,K., Kitai,Y., Toriu,N., Yamamoto,S., Mizuta,K.,
            Saitou,M., Tsukiyama,T., Taniuchi,I., Osato,M. and Yanagita,M.
  TITLE     Expression pattern of Runt-related transcription factor (RUNX)
            family members and the role of RUNX1 during kidney development
  JOURNAL   Biochem Biophys Res Commun 722, 150155 (2024)
   PUBMED   38795454
REFERENCE   3  (bases 1 to 5661)
  AUTHORS   Lemberg,K., Mertens,N.D., Yousef,K., Schneider,R., Merz,L.M.,
            Mansour,B., Salmanullah,D., Kolvenbach,C.M., Saida,K., Yu,S.,
            Holzel,S., Steinsapir,A., Goncalves,K.A., Nicolas Frank,C.,
            Franken,G.A.C., Shril,S., Buerger,F. and Hildebrandt,F.
  TITLE     Quantifiable and reproducible phenotypic assessment of a
            constitutive knockout mouse model for congenital nephrotic syndrome
            of the Finnish type
  JOURNAL   Sci Rep 14 (1), 15916 (2024)
   PUBMED   38987283
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5661)
  AUTHORS   Williamson,C.R. and Jones,N.
  TITLE     Reduced Nephrin Tyrosine Phosphorylation Enhances Insulin Secretion
            and Increases Glucose Tolerance With Age
  JOURNAL   Endocrinology 165 (8) (2024)
   PUBMED   38954536
REFERENCE   5  (bases 1 to 5661)
  AUTHORS   Cunanan,J., Rajyam,S.S., Sharif,B., Udwan,K., Rana,A., De
            Gregorio,V., Ricardo,S., Elia,A., Brooks,B., Weins,A., Pollak,M.,
            John,R. and Barua,M.
  TITLE     Mice with a Pax2 missense variant display impaired glomerular
            repair
  JOURNAL   Am J Physiol Renal Physiol 326 (5), F704-F726 (2024)
   PUBMED   38482556
REFERENCE   6  (bases 1 to 5661)
  AUTHORS   Doyonnas,R., Kershaw,D.B., Duhme,C., Merkens,H., Chelliah,S.,
            Graf,T. and McNagny,K.M.
  TITLE     Anuria, omphalocele, and perinatal lethality in mice lacking the
            CD34-related protein podocalyxin
  JOURNAL   J Exp Med 194 (1), 13-27 (2001)
   PUBMED   11435469
REFERENCE   7  (bases 1 to 5661)
  AUTHORS   Putaala,H., Soininen,R., Kilpelainen,P., Wartiovaara,J. and
            Tryggvason,K.
  TITLE     The murine nephrin gene is specifically expressed in kidney, brain
            and pancreas: inactivation of the gene leads to massive proteinuria
            and neonatal death
  JOURNAL   Hum Mol Genet 10 (1), 1-8 (2001)
   PUBMED   11136707
REFERENCE   8  (bases 1 to 5661)
  AUTHORS   Moeller,M.J., Kovari,I.A. and Holzman,L.B.
  TITLE     Evaluation of a new tool for exploring podocyte biology: mouse
            Nphs1 5' flanking region drives LacZ expression in podocytes
  JOURNAL   J Am Soc Nephrol 11 (12), 2306-2314 (2000)
   PUBMED   11095653
REFERENCE   9  (bases 1 to 5661)
  AUTHORS   Putaala,H., Sainio,K., Sariola,H. and Tryggvason,K.
  TITLE     Primary structure of mouse and rat nephrin cDNA and structure and
            expression of the mouse gene
  JOURNAL   J Am Soc Nephrol 11 (6), 991-1001 (2000)
   PUBMED   10820162
REFERENCE   10 (bases 1 to 5661)
  AUTHORS   Holzman,L.B., St John,P.L., Kovari,I.A., Verma,R., Holthofer,H. and
            Abrahamson,D.R.
  TITLE     Nephrin localizes to the slit pore of the glomerular epithelial
            cell
  JOURNAL   Kidney Int 56 (4), 1481-1491 (1999)
   PUBMED   10504499
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF191090.1, AF172256.1, AK141081.1 and AC167970.4.
            
            On Jan 4, 2007 this sequence version replaced NM_019459.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF172256.1, AF191090.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-212               AF191090.1         329-540
            213-272             AF172256.1         213-272
            273-3288            AF191090.1         601-3616
            3289-3757           AF172256.1         3289-3757
            3758-4101           AK141081.1         4053-4396
            4102-5661           AC167970.4         131682-133241       c
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 18.36 cM"
     gene            1..5661
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /note="nephrosis 1, nephrin"
                     /db_xref="GeneID:54631"
                     /db_xref="MGI:MGI:1859637"
     exon            1..186
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..3857
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /note="nephrin 1; renal glomerulus-specific cell adhesion
                     receptor; nephrosis 1 homolog, nephrin"
                     /codon_start=1
                     /product="nephrin precursor"
                     /protein_id="NP_062332.2"
                     /db_xref="CCDS:CCDS39884.1"
                     /db_xref="GeneID:54631"
                     /db_xref="MGI:MGI:1859637"
                     /translation="MGAKEATVRGPGASPVHRTCHLIPLLLAGMLTTGLAQSPVPTSA
                     PRGFWALSENLTVVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGD
                     SAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGS
                     TVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARV
                     TPQSSDNGQLLVCEGSNPALATPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLE
                     LPCIARGGNPPATLQWLKNGKPVSIAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSY
                     NSVSAETQERSITLQVTFPPSAVTILGSTSQSENKNVTLCCLTKSSRPRVLLRWWLGG
                     RQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLN
                     VKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEP
                     RRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQLSASTQLVVQFPPTNLTILA
                     NSSALRPGDALNLTCVSISSNPPVNLSLDKEGERLDDVAAKPQSAPFKGSAASRSVFL
                     RVSSRDHGHRVTCRAHSEALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQALLPV
                     SVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEG
                     TAEALLKLDVHYAPTIRALKDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEDEEE
                     LNLDDMEKMSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDH
                     PTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHKYHQGVVH
                     SSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVG
                     LEWKPGFDGGLPQRFQIRYEALESPGFLYMDVLPAQATTFTLTGLKPSTRYRIWLLAS
                     NALGDSGLTDKGIQVSITTPGLDQAPEDTDQPLPTEQPPGPPRLPLLPVLFAVGGLLL
                     LSNASCVGGLLWRRRLRRLAEEISEKTEAGSEEDRIRNEYEESQWTGDRDTRSSTVST
                     AEVDPHYYSMRDFSPQLPPTLEEVSYRQAFTGIEDEDMAFPGHLYDEVERVYGPPGVW
                     GPLYDEVQMDPYDLRWPEVKYEDPRGIYDQVAADMDAGEPGSLPFELRGHLV"
     exon            187..402
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            403..525
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            526..654
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            655..736
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            737..840
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            841..968
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1140
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1298
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1443
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1568
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1755
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1885
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2058
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..2199
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2340
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            2341..2462
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            2463..2634
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            2635..2791
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            2792..2943
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            2944..3055
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3237
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            3238..3294
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            3295..3414
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            3415..3439
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            3440..3515
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            3516..3609
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            3610..3725
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            3726..3890
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     exon            3891..5661
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4085..4090
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /note="hexamer: ATTAAA"
     polyA_site      4106
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
     regulatory      4271..4276
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /note="hexamer: AATAAA"
     polyA_site      4305
                     /gene="Nphs1"
                     /gene_synonym="nephrin; NephrinB"
                     /note="major polyA site"
ORIGIN      
        1 gcgagttaga aagagattga aagaacagaa aagcagggca cacagagaga ggctcaggga
       61 agacagcaac aaacaagctg ctggtgatgg gagctaagga agccacagtc agaggccccg
      121 gggcaagccc agtgcacaga acttgccacc tgattcccct gctgctcgcg ggaatgctga
      181 ccacaggcct ggcccagtcg ccagtcccca cctcagcacc tcgaggcttc tgggctctat
      241 ctgaaaacct gactgtggtg gaagggtcga cagttaagct gtggtgtggc gtcagggccc
      301 ccggcagtgt ggtgcagtgg gctaaggatg ggctgcttct gggtccaaac cctaagattc
      361 caggcttccc aaggtacagc ctggaaggag acagtgctaa aggtgagttc cacctgctta
      421 ttgaagcctg tgacctcagc gatgatgcgg agtacgagtg ccaagtcggc cgctccgagt
      481 tgggtcccga gctcgtgtct cccagcgtaa tcctctccat cctagtttcc cccaaggtgc
      541 ttcagttaac ccctgaggca ggaagcacag ttacctgggt agctggacag gaatatgtgg
      601 tcacctgtgt gtctggggat gcaaagccag cacctgacat catcttcatc cagggtggac
      661 gcacggtaga ggatgtctcc tccagcgtga atgagggatc agaggagaaa ctcttcttca
      721 cggaagccga agccagggtg acaccccaga gctcagataa tgggcagctg ctagtctgcg
      781 aggggtccaa cccagccttg gccactccca taaaggcttc gttcaccatg aatatcctgt
      841 tccccccagg acctcctgtc attgattggc caggcctgaa tgaggggcat gtacgggcag
      901 gggagaacct ggagctaccc tgcatagcca gaggtggaaa tccacctgcg accctgcagt
      961 ggctgaagaa tggtaaacca gtgtccatag cttggggcac agagcatgcc caggcagtgg
     1021 ctcacagtgt gctggtgatg accgttcgac ctgaagacca cggagctcgg ctcagctgtc
     1081 agtcctacaa cagtgtgtct gcagagaccc aggagcgaag catcactctg caggtcacct
     1141 ttccccccag tgccgttacc atcctgggat ctacatcaca gtctgagaac aaaaatgtga
     1201 ccctttgctg ccttaccaag tccagtcgcc cacgggtcct gctgcgatgg tggttgggtg
     1261 gacggcagtt gctgcccacg gatgagacag tcatggatgg cctgcatggt ggccacatct
     1321 ccatgtccaa tctgacactc ttggtgaaga gagaagacaa tggcctgtcc ctcacctgcg
     1381 aagccttcag tgatgccttc agcaaggaga ccttcaagaa gtcactcacc ttgaatgtaa
     1441 aatacccggc ccagaagctg tggattgagg gacccccaga ggggcagagc atccggactg
     1501 gaactcgggt gaggctggta tgcttggcca ttggaggcaa cccagagccc tccctcacct
     1561 ggcttaagga ttcgcgcccg gtgaacgatc ctcggcagtc tcaggagccc cggcgtgtgc
     1621 agctgggcag tgtggagaag tccggcagca ctttctcccg cgagctggtg ctgatcatag
     1681 gcccgccgga caacctagcc aagttctcct gcaaggcggg tcagctcagt gcgtctacgc
     1741 agctggtggt gcagttcccc ccaacaaacc tgaccatcct ggccaactcg tccgcactgc
     1801 gcccaggcga cgccttgaac ttgacctgcg tcagcatcag cagcaaccct ccagttaact
     1861 tgtctttgga caaggaagga gagaggctgg acgacgtggc tgcaaaaccc cagagcgccc
     1921 cattcaaagg ctccgctgca tccaggagtg tttttcttcg ggtgtcatcc cgagaccacg
     1981 gtcaccgggt tacctgccgg gcccacagcg aggcacttcg tgaaaccgtg agctctttct
     2041 accgcctcaa cgtgttatac cctccagagt tcctgggaga gcaagtgcgg gcagtgaccg
     2101 tggtggagca gggccaggca ctgctgcctg tgtccgtgtc tgctaacccc gcccccgagg
     2161 ccttcaactg gaccttccga ggctaccgcc tcagcccagc tgggggtccc cggcaccgca
     2221 tcctgtctgg aggggcactg cagctgtgga atgtaacccg agctgacgat ggcttttatc
     2281 agctacactg ccagaactcg gagggcaccg ccgaggcgct gttgaagctg gacgtgcatt
     2341 atgctcccac catccgtgcc ctgaaggacc ctactgaggt gaatgttggg ggttctgtgg
     2401 atatagtctg caccgtcgat gccaatccca tcctcccaga gatgttcagc tgggagagac
     2461 tgggggaaga cgaggaggaa ctgaacctgg acgacatgga gaagatgtca aagggatcca
     2521 cagggcgtct gcggattcgc caggccaagc tgtcccaggc tggcgcttac cagtgcatcg
     2581 tggacaacgg ggtggctccc gcagccagag gactggttcg tcttgttgtc cgatttgccc
     2641 cccaggtgga tcatcctact cccctgacga aagtggctgc tgctggggac agcaccagct
     2701 cagccacact ccactgccgt gcccgggggg tccccaacat cgacttcact tggaccaaaa
     2761 atggggtccc tctggatctc caagacccca ggtacacaga gcacaagtac caccagggtg
     2821 tggtccacag cagcctcctg accattgcta atgtgtctgc agcccaggac tatgccctct
     2881 tcaaatgcac agccaccaat gcccttggtt cagaccacac caacatccag ctcgtcagca
     2941 tcagccgtcc tgaccctcca ctggggctga aggttgtgag cgtgagccct cactcggtag
     3001 gactggagtg gaaacctggc tttgatgggg gtctgcctca gaggttccaa atcaggtatg
     3061 aggccctcga gagcccagga ttcctctaca tggatgtcct gcctgcacag gccaccacct
     3121 tcacactgac tgggctgaag ccttctacac ggtacaggat ctggctgttg gccagcaatg
     3181 ccctggggga cagtggattg acggacaagg ggatccaggt ctccatcact accccaggcc
     3241 tggaccaggc tcctgaagac acagaccagc cgctgcccac agagcagcct ccgggacccc
     3301 cgaggctgcc cctgctgcct gtgctctttg cggttggtgg tcttctgttg ctttccaatg
     3361 cctcctgtgt tgggggactc ctctggcgga gaagactgag gcgccttgct gaggagatct
     3421 cagagaagac agaggcaggg tcggaggagg atcgaatcag gaatgaatat gaggagagtc
     3481 agtggactgg ggaccgggac acaagaagct ccacggttag cacagcagaa gtggacccac
     3541 actactactc catgagggac ttcagtcccc agcttccccc aacactggaa gaggtgtcat
     3601 atcgccaagc cttcacaggt attgaagatg aggacatggc cttccccgga cacctgtatg
     3661 acgaggtgga gagagtctat ggcccacctg gggtctgggg acccctctat gatgaagtac
     3721 aaatggaccc ctatgacctt cgctggcctg aggtcaaata tgaggaccca aggggaatct
     3781 acgaccaggt ggcggcagac atggatgctg gggagcctgg ttctctgccc tttgagctga
     3841 ggggacatct ggtgtgagac tcttcacgac acctgtttcc tacagccttg gagaagacct
     3901 gaggaaatcc gtacaaatga caagaagact ccagctgaca tctgggatga ccaagactcc
     3961 tttcattgta atggggcttc tcccaagaga cacaatcatt tggaaatgac atttgaacaa
     4021 tgaaaatcta tttagggatc attatacttt tcaaaagtaa aaattttaga agtaggaaag
     4081 atctattaaa aatcctattc caaggggctg gagagatggc tcagtggtta agagcactga
     4141 ctgctcttcc agaggtcctg agttcaattc ccagcaacca catggtggct cacaaccatc
     4201 cttaatgaga tatgatgccc atttctggtg tgtctgaaga cagttacagt atactcatat
     4261 acataaaata aataaatctt taaaaaattt ctgttccaag ccaaaggatg ccccagtggg
     4321 agaacatatg tttagcacgt gtgagatttt gggttgcagg ttgcagccag ccctggcatt
     4381 acatgcacat acaaagctgg acatgctgac ccatccctgt catcccagct ctgtgggagg
     4441 ctgaggcagg ggaagcacaa gttcaaagcc acttaggaac tggaaagatg ggtcaagagt
     4501 tgagagctct aacagtagct actgcccttg cagaggccct gactttggtt cccagcaccc
     4561 agatcaggtg gctcaccgtc acattttcat ccctagggaa tttgatgctt tcttctgacc
     4621 tccaggagca cctgcactcc tgtacacata gacacatata tacacaatta caaataagat
     4681 aaatccaacc tgtgtggtgg cccacacctt taatcctagc agaatttgag aagcagaagc
     4741 aggggggatt tctataagtt taaggcttac aacttagtga gttttgagct acatagtgaa
     4801 accccgactc aaatgttaat tttaaaagct tcagaattaa aagggggcat gactcagtgg
     4861 tagagcccct gcctagaatc ccccagtgag gggctggggg cgtggctcag tggtagagcc
     4921 cctgcctgga atcccccagt gaggggctgg gatgtggctc agtggtagag cccctgccta
     4981 gaatccccag tgagaggctg ggggcgtggc tcagtggtag agcacttgcc tggcatgctc
     5041 aaagctctga attctatccc aaaaacaaaa caaaaacccc agtccatttt aagttcagtt
     5101 acatatgaaa tagttaatat taataagcaa aattatagac cattgtcaga tctcagctgg
     5161 ttttgtttta ttttccacag tttgtgaaac ataaacgaac tgatgcatgc aagaaggaac
     5221 atattgtagc aaaaggatat agttgtcaaa gaatggacat ttatgggcaa agcttagaga
     5281 ccacgcacta gtgtatactt agaattagag ggtatggtaa cttcaattta ttttcaaaat
     5341 taaaaaaatg cacgtttata gatacagaaa agggtgagct cagactcaaa tgttgacact
     5401 ggaggaatgt aggtaatgca gaatgaaagt taaaattgaa gagttgatgg accgataagt
     5461 gatgacgtca cagaagcaag aacacaattg gcggctgggt cttaggggcc gtggaattct
     5521 agatgttcca ctttggaaaa aaaatattca gaacacactt atttgcttgt ctctctgtgt
     5581 gtgtgtgtgt gtgcacgcgc gtgccacata tacacgccca cacgcctgac agtgtatgcc
     5641 acacaaaata aataaaatgt a
//
