LOCUS       NM_008942               4170 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus aminopeptidase puromycin sensitive (Npepps), mRNA.
ACCESSION   NM_008942
VERSION     NM_008942.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Huang,B., Xiong,X., Zhang,L., Liu,X., Wang,Y., Gong,X., Sang,Q.,
            Lu,Y., Qu,H., Zheng,H. and Zheng,Y.
  TITLE     PSA controls hepatic lipid metabolism by regulating the NRF2
            signaling pathway
  JOURNAL   J Mol Cell Biol 13 (7), 527-539 (2021)
   PUBMED   34048566
  REMARK    GeneRIF: PSA controls hepatic lipid metabolism by regulating the
            NRF2 signaling pathway.
REFERENCE   3  (bases 1 to 4170)
  AUTHORS   Huber,R.J. and O'Day,D.H.
  TITLE     Nucleocytoplasmic transfer of cyclin dependent kinase 5 and its
            binding to puromycin-sensitive aminopeptidase in Dictyostelium
            discoideum
  JOURNAL   Histochem Cell Biol 136 (2), 177-189 (2011)
   PUBMED   21766205
  REMARK    GeneRIF: Nucleocytoplasmic transfer of cyclin dependent kinase 5
            and its binding to puromycin-sensitive aminopeptidase in
            Dictyostelium discoideum
REFERENCE   4  (bases 1 to 4170)
  AUTHORS   Menzies,F.M., Hourez,R., Imarisio,S., Raspe,M., Sadiq,O.,
            Chandraratna,D., O'Kane,C., Rock,K.L., Reits,E., Goldberg,A.L. and
            Rubinsztein,D.C.
  TITLE     Puromycin-sensitive aminopeptidase protects against
            aggregation-prone proteins via autophagy
  JOURNAL   Hum Mol Genet 19 (23), 4573-4586 (2010)
   PUBMED   20829225
  REMARK    GeneRIF: findings show that by promoting autophagic protein
            clearance, PSA helps protect against accumulation of
            aggregation-prone proteins and proteotoxicity
REFERENCE   5  (bases 1 to 4170)
  AUTHORS   Towne,C.F., York,I.A., Neijssen,J., Karow,M.L., Murphy,A.J.,
            Valenzuela,D.M., Yancopoulos,G.D., Neefjes,J.J. and Rock,K.L.
  TITLE     Puromycin-sensitive aminopeptidase limits MHC class I presentation
            in dendritic cells but does not affect CD8 T cell responses during
            viral infections
  JOURNAL   J Immunol 180 (3), 1704-1712 (2008)
   PUBMED   18209067
  REMARK    GeneRIF: These data indicate that PSA may play a role in limiting
            MHC class I Ag presentation in dendritic cells in vivo but that it
            is not essential for generating most MHC class I-presented peptides
            or for stimulating CTL responses to several Ags.
REFERENCE   6  (bases 1 to 4170)
  AUTHORS   Osada,T., Watanabe,G., Sakaki,Y. and Takeuchi,T.
  TITLE     Puromycin-sensitive aminopeptidase is essential for the maternal
            recognition of pregnancy in mice
  JOURNAL   Mol Endocrinol 15 (6), 882-893 (2001)
   PUBMED   11376108
REFERENCE   7  (bases 1 to 4170)
  AUTHORS   Osada,T., Ikegami,S., Takiguchi-Hayashi,K., Yamazaki,Y.,
            Katoh-Fukui,Y., Higashinakagawa,T., Sakaki,Y. and Takeuchi,T.
  TITLE     Increased anxiety and impaired pain response in puromycin-sensitive
            aminopeptidase gene-deficient mice obtained by a mouse gene-trap
            method
  JOURNAL   J Neurosci 19 (14), 6068-6078 (1999)
   PUBMED   10407043
REFERENCE   8  (bases 1 to 4170)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   9  (bases 1 to 4170)
  AUTHORS   Osada,T., Sakaki,Y. and Takeuchi,T.
  TITLE     Puromycin-sensitive aminopeptidase gene (Psa) maps to mouse
            chromosome 11
  JOURNAL   Genomics 56 (3), 361-362 (1999)
   PUBMED   10087210
REFERENCE   10 (bases 1 to 4170)
  AUTHORS   Constam,D.B., Tobler,A.R., Rensing-Ehl,A., Kemler,I., Hersh,L.B.
            and Fontana,A.
  TITLE     Puromycin-sensitive aminopeptidase. Sequence analysis, expression,
            and functional characterization
  JOURNAL   J Biol Chem 270 (45), 26931-26939 (1995)
   PUBMED   7592939
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC098212.1 and AI839456.1.
            
            On Feb 22, 2018 this sequence version replaced NM_008942.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098212.1, SRR1660811.1369.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4143              BC098212.1         1-4143
            4144-4170           AI839456.1         1-27                c
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 60.95 cM"
     gene            1..4170
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /note="aminopeptidase puromycin sensitive"
                     /db_xref="GeneID:19155"
                     /db_xref="MGI:MGI:1101358"
     exon            1..376
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     CDS             119..2881
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /EC_number="3.4.11.14"
                     /note="cytosol alanyl aminopeptidase"
                     /codon_start=1
                     /product="puromycin-sensitive aminopeptidase"
                     /protein_id="NP_032968.2"
                     /db_xref="CCDS:CCDS25317.1"
                     /db_xref="GeneID:19155"
                     /db_xref="MGI:MGI:1101358"
                     /translation="MWLAAAVPSLARRLLLLGPPPPPLLLLLSRSSRRRRRLHSLGLA
                     AMPEKRPFERLPAEVSPINYSLCLKPDLLDFTFEGKLEAAAQVRQATNQIVMNCADID
                     IITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTGTGTLKIDFVGELNDKMKGF
                     YRSRYTTPAGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMN
                     VIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKA
                     EQGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLI
                     DPKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFP
                     EYDIWTQFVSADYTRAQELDALDNSHPIEVSVGHPSEVDEIFDAISYSKGASVIRMLH
                     DYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLESASGKPIAAVMNTWTKQMGFPLIY
                     VEAEQVEDDRVLKLSQKKFCASGPYGGEDCPQWMVPITISTSEDPNQAKLKILMDKPE
                     MSVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLF
                     SLARAGIISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKD
                     VFSPIGERLGWDPKPGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFKEHVEGKQIL
                     SADLRSPVYLTVLKHGDGATLDIMLKLHKQADMQEEKNRIERVLGATLSPELIQKVLT
                     FALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELHNRYQGGFLISRLIKLS
                     VEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDADSIHQYLLQRK
                     TSPPSV"
     exon            377..461
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            462..539
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            540..661
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            662..769
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            770..970
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1067
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1101
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1216
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1381
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1486
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1547
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1657
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1721
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1861
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..1996
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            1997..2216
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2359
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2416
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2524
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            2525..2680
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            2681..2728
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     exon            2729..4155
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4116..4121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /note="hexamer: ATTAAA"
     regulatory      4136..4141
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /note="hexamer: AATAAA"
     polyA_site      4152
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
     polyA_site      4155
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /note="major polyA site"
ORIGIN      
        1 gtaggcagcc cgcccgcacc gccgcccgcc cgcccgcccg gcccaggcac ccgctgggtc
       61 tgtcccccgc cccagcctcc ccggcggctc tcggcaggcg gtcgcccgcg cccggtgaat
      121 gtggctggca gccgccgtcc cctccctcgc tcgccgcctg ctcctcctcg gccctccgcc
      181 tcctcccctc cttctcctcc tcagccgctc ctctcgccgc cgccgccgcc tccacagcct
      241 gggcctcgcc gcgatgccgg agaagcggcc cttcgagcgg ctgcccgccg aggtgtcgcc
      301 catcaactac agcctctgcc tcaagcccga tttgctggac ttcaccttcg agggcaagct
      361 ggaggccgcc gcccaggtaa ggcaggcaac caatcaaatt gtgatgaatt gtgctgatat
      421 tgacattatt acagcttcat atgccccaga aggagatgaa gaaatccatg cgacaggatt
      481 taactatcag aatgaagatg agaaagtcac cttgtctttt cctagcactc tgcaaacagg
      541 tacaggaacc ttaaagatag attttgttgg agagctgaat gacaaaatga aaggtttcta
      601 tagaagcaga tacaccaccc ctgccggcga ggtgcgctat gctgctgtca cacagtttga
      661 ggctactgat gcccgaaggg cttttccttg ctgggatgag cctgctatca aagcaacttt
      721 tgatatctcg ctggttgtgc ctaaagacag agtggcttta tcaaatatga atgtaattga
      781 caggaaacca tatcctgatg atgaaaattt agtggaagtg aagtttgctc gcacacctgt
      841 tatgtctacg tatctggtgg catttgttgt gggtgaatat gactttgtag aaacaaggtc
      901 aaaagatggt gtgtgtgtcc gtgtttacac ccctgttggc aaagcagagc aaggaaagtt
      961 tgcgctcgag gttgctgcta agaccttgcc tttttataaa gactacttca atgttcctta
     1021 tcctctacct aaaattgatc tcattgctat tgctgacttt gcagctggtg ccatggagaa
     1081 ctggggcctt gttacttata gggaaacggc cttgcttatt gatccaaaaa actcgtgttc
     1141 ttcatcacgc cagtgggttg ctctggttgt gggacatgaa ctcgcccatc aatggtttgg
     1201 aaatcttgtt actatggaat ggtggactca tctctggttg aatgaaggct ttgcatcctg
     1261 gattgagtat ctttgtgtag accactgctt tccagagtat gatatctgga ctcagtttgt
     1321 ttctgcagat tatacccgtg cccaggaact tgatgcctta gataacagcc atcctattga
     1381 agtcagtgtg ggccatccgt ctgaggttga tgagatattt gatgctatat catatagcaa
     1441 aggtgcatct gtaatccgaa tgctacatga ctacattggt gataaggact ttaagaaagg
     1501 aatgaatatg tatttaacca agttccaaca aaagaatgct gccacagagg atctctggga
     1561 aagtttggaa agtgccagtg gcaaacccat agcagctgtg atgaatacct ggaccaaaca
     1621 aatgggattc cctctcattt atgtggaagc tgaacaggta gaagatgaca gagtgctgaa
     1681 gctgtctcag aagaagtttt gtgccagtgg accatatggc ggtgaagact gtcctcagtg
     1741 gatggttcct atcacaattt caactagtga ggatcctaac caggctaagc tgaaaatact
     1801 aatggataag ccagagatga gtgtggtttt gaaaaatgtc aaaccagacc aatgggtaaa
     1861 gctaaatctg ggaacagttg ggttttatcg aacccagtac agctctgcca tgctcgaaag
     1921 tttattacca ggcatccgtg acctttctct gcccccagtg gatcgacttg gattacagaa
     1981 tgacctcttt tctctggctc gagctggcat cattagcact gtagaggttc taaaagtcat
     2041 ggaggctttt gtgaatgagc ccaattatac tgtatggagc gacctgagct gtaacctggg
     2101 gattctttca actctcttgt cccacacaga cttctatgag gaaatccagg agtttgtcaa
     2161 agatgtcttt tcacctatag gagagagatt gggctgggac cccaaacctg gagaaggtca
     2221 tctagacgca ctcctgaggg gcttggtgct gggcaaactg ggaaaagcag gccataaggc
     2281 aactttggaa gaagcccgtc gtcggtttaa ggagcacgtg gaagggaaac agattctttc
     2341 tgctgaccta aggagtcctg tctatctcac tgttttaaag catggggatg gcgctacctt
     2401 agatatcatg ctgaagcttc acaaacaagc tgatatgcaa gaagagaaaa acagaattga
     2461 aagagttctt ggggctactc tttcacctga actgattcaa aaagtcctta cttttgcact
     2521 ttcagaagag gtccgtccgc aggacactgt gtcggtgatt ggtggagtgg ctggaggcag
     2581 caagcatggg aggaaagctg cttggaaatt catcaaggac aactgggagg agcttcacaa
     2641 ccggtaccag ggagggttct taatatccag actaataaag ctctcagttg aggggtttgc
     2701 agttgataaa atggctggag aagttaaggc tttcttcgag agtcacccag ctccttcagc
     2761 tgagcgcacc atccagcagt gttgtgaaaa tatcctgttg aatgctgctt ggctcaagcg
     2821 agatgctgac agcattcacc agtacctcct tcagcggaaa acctccccac cctcggtgtg
     2881 aggcctcctc cgcacctggg cctcactgcc tctctgcagg gatgaggtgg agctaccaaa
     2941 cagctgactc acatgccaag aatctggagt cttacaccag tgggggttgg acaatgaatg
     3001 tagtaggtcc ctgctcacac tccagaatta aattctattg aaaaaggaaa atcagcagtt
     3061 cagcaaaaaa ataaaataaa ataaaataaa aaataaaata aaaatgtaaa tatgatagta
     3121 ataaactaga gcataacgaa actgtgaaac ttcctgaagc ctgtcatggt caaagtgttg
     3181 aacactctcc tgttagtgac ggatattgtt ttatagccta ccagaaggaa agaggctttg
     3241 ggggaagagg gaagagggtt ttgtgaagtg ggtgtctgca gcctgacaag ccgggtatgt
     3301 ccatcactgg ggatgctgac caaaaggctg atagctggct cacatagtgg agcctgtgca
     3361 gaattataag tgggttttct ttttcctttt tggcatggga accgaccagg aaggtatctt
     3421 ttcctgtata actcaatctg aaccaaggat tgtagttccc tccctgcctc ccttctgtgt
     3481 gatccccaac cccccaaaaa gtaatgaaaa gctttaaaaa acaaaacaaa acaaaaaaaa
     3541 aacacaccct ccctcccttc ccgggttctt ctccctttag ttctgcccag ccctgacctg
     3601 tccttagata acagaaacat ctttgatggc cacattaaat aagaggagct gatatgcttt
     3661 tatttcctat gataaccttc aaggaaccct gacatgcata agaggtgaaa gagtagaaat
     3721 aaagattatc agctaggcct tctgctcaaa actgtccggt tacctgacag gccaaatgct
     3781 gcacctcttc cgatcagtat cagtgagcct ggctcaccac ttgtgcatat gaggggccag
     3841 tggggactgc tgtggggccc agatgtccgc tgcaccgctg tgtcgccagt gtgctcacta
     3901 acaagccccg ccacctaacc cagtgtgttt ttactaagtc tcagcccttt cccccacccc
     3961 tttttcttct ctcttccttt tatttttttc tgaatccttt tattttgtgt taacagaaat
     4021 tcatatttgg tgtggcttaa tgtattttgg aaggtcatca gattgtgaga ctgcttcctt
     4081 gaaacatttt tgtgctattg ttttaaaaaa taataattaa aaaacagttg gcgttaataa
     4141 aaatgtcaat gtgaaaaaaa aaaaaaaaaa
//
