LOCUS       NP_032968                920 aa            linear   ROD 28-APR-2025
DEFINITION  puromycin-sensitive aminopeptidase [Mus musculus].
ACCESSION   NP_032968
VERSION     NP_032968.2
DBSOURCE    REFSEQ: accession NM_008942.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 920)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 920)
  AUTHORS   Huang,B., Xiong,X., Zhang,L., Liu,X., Wang,Y., Gong,X., Sang,Q.,
            Lu,Y., Qu,H., Zheng,H. and Zheng,Y.
  TITLE     PSA controls hepatic lipid metabolism by regulating the NRF2
            signaling pathway
  JOURNAL   J Mol Cell Biol 13 (7), 527-539 (2021)
   PUBMED   34048566
  REMARK    GeneRIF: PSA controls hepatic lipid metabolism by regulating the
            NRF2 signaling pathway.
REFERENCE   3  (residues 1 to 920)
  AUTHORS   Huber,R.J. and O'Day,D.H.
  TITLE     Nucleocytoplasmic transfer of cyclin dependent kinase 5 and its
            binding to puromycin-sensitive aminopeptidase in Dictyostelium
            discoideum
  JOURNAL   Histochem Cell Biol 136 (2), 177-189 (2011)
   PUBMED   21766205
  REMARK    GeneRIF: Nucleocytoplasmic transfer of cyclin dependent kinase 5
            and its binding to puromycin-sensitive aminopeptidase in
            Dictyostelium discoideum
REFERENCE   4  (residues 1 to 920)
  AUTHORS   Menzies,F.M., Hourez,R., Imarisio,S., Raspe,M., Sadiq,O.,
            Chandraratna,D., O'Kane,C., Rock,K.L., Reits,E., Goldberg,A.L. and
            Rubinsztein,D.C.
  TITLE     Puromycin-sensitive aminopeptidase protects against
            aggregation-prone proteins via autophagy
  JOURNAL   Hum Mol Genet 19 (23), 4573-4586 (2010)
   PUBMED   20829225
  REMARK    GeneRIF: findings show that by promoting autophagic protein
            clearance, PSA helps protect against accumulation of
            aggregation-prone proteins and proteotoxicity
REFERENCE   5  (residues 1 to 920)
  AUTHORS   Towne,C.F., York,I.A., Neijssen,J., Karow,M.L., Murphy,A.J.,
            Valenzuela,D.M., Yancopoulos,G.D., Neefjes,J.J. and Rock,K.L.
  TITLE     Puromycin-sensitive aminopeptidase limits MHC class I presentation
            in dendritic cells but does not affect CD8 T cell responses during
            viral infections
  JOURNAL   J Immunol 180 (3), 1704-1712 (2008)
   PUBMED   18209067
  REMARK    GeneRIF: These data indicate that PSA may play a role in limiting
            MHC class I Ag presentation in dendritic cells in vivo but that it
            is not essential for generating most MHC class I-presented peptides
            or for stimulating CTL responses to several Ags.
REFERENCE   6  (residues 1 to 920)
  AUTHORS   Osada,T., Watanabe,G., Sakaki,Y. and Takeuchi,T.
  TITLE     Puromycin-sensitive aminopeptidase is essential for the maternal
            recognition of pregnancy in mice
  JOURNAL   Mol Endocrinol 15 (6), 882-893 (2001)
   PUBMED   11376108
REFERENCE   7  (residues 1 to 920)
  AUTHORS   Osada,T., Ikegami,S., Takiguchi-Hayashi,K., Yamazaki,Y.,
            Katoh-Fukui,Y., Higashinakagawa,T., Sakaki,Y. and Takeuchi,T.
  TITLE     Increased anxiety and impaired pain response in puromycin-sensitive
            aminopeptidase gene-deficient mice obtained by a mouse gene-trap
            method
  JOURNAL   J Neurosci 19 (14), 6068-6078 (1999)
   PUBMED   10407043
REFERENCE   8  (residues 1 to 920)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   9  (residues 1 to 920)
  AUTHORS   Osada,T., Sakaki,Y. and Takeuchi,T.
  TITLE     Puromycin-sensitive aminopeptidase gene (Psa) maps to mouse
            chromosome 11
  JOURNAL   Genomics 56 (3), 361-362 (1999)
   PUBMED   10087210
REFERENCE   10 (residues 1 to 920)
  AUTHORS   Constam,D.B., Tobler,A.R., Rensing-Ehl,A., Kemler,I., Hersh,L.B.
            and Fontana,A.
  TITLE     Puromycin-sensitive aminopeptidase. Sequence analysis, expression,
            and functional characterization
  JOURNAL   J Biol Chem 270 (45), 26931-26939 (1995)
   PUBMED   7592939
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC098212.1 and AI839456.1.
            
            On Jun 26, 2005 this sequence version replaced NP_032968.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098212.1, SRR1660811.1369.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 60.95 cM"
     Protein         1..920
                     /product="puromycin-sensitive aminopeptidase"
                     /EC_number="3.4.11.14"
                     /note="cytosol alanyl aminopeptidase"
                     /calculated_mol_wt=103195
     Region          62..500
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(179,181,317..320,353..354,357,376,380,434,439)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Site            439
                     /site_type="other"
                     /note="Transition state stabilizer. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q11011.2)"
     Region          579..892
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     Region          727..731
                     /region_name="Nuclear localization signal.
                     /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q11011.2)"
     CDS             1..920
                     /gene="Npepps"
                     /gene_synonym="AAP-S; goku; MP100; Psa"
                     /coded_by="NM_008942.3:119..2881"
                     /db_xref="CCDS:CCDS25317.1"
                     /db_xref="GeneID:19155"
                     /db_xref="MGI:MGI:1101358"
ORIGIN      
        1 mwlaaavpsl arrllllgpp ppplllllsr ssrrrrrlhs lglaampekr pferlpaevs
       61 pinyslclkp dlldftfegk leaaaqvrqa tnqivmncad idiitasyap egdeeihatg
      121 fnyqnedekv tlsfpstlqt gtgtlkidfv gelndkmkgf yrsryttpag evryaavtqf
      181 eatdarrafp cwdepaikat fdislvvpkd rvalsnmnvi drkpypdden lvevkfartp
      241 vmstylvafv vgeydfvetr skdgvcvrvy tpvgkaeqgk falevaaktl pfykdyfnvp
      301 yplpkidlia iadfaagame nwglvtyret allidpknsc sssrqwvalv vghelahqwf
      361 gnlvtmewwt hlwlnegfas wieylcvdhc fpeydiwtqf vsadytraqe ldaldnshpi
      421 evsvghpsev deifdaisys kgasvirmlh dyigdkdfkk gmnmyltkfq qknaatedlw
      481 eslesasgkp iaavmntwtk qmgfpliyve aeqveddrvl klsqkkfcas gpyggedcpq
      541 wmvpitists edpnqaklki lmdkpemsvv lknvkpdqwv klnlgtvgfy rtqyssamle
      601 sllpgirdls lppvdrlglq ndlfslarag iistvevlkv meafvnepny tvwsdlscnl
      661 gilstllsht dfyeeiqefv kdvfspiger lgwdpkpgeg hldallrglv lgklgkaghk
      721 atleearrrf kehvegkqil sadlrspvyl tvlkhgdgat ldimlklhkq admqeeknri
      781 ervlgatlsp eliqkvltfa lseevrpqdt vsviggvagg skhgrkaawk fikdnweelh
      841 nryqggflis rliklsvegf avdkmagevk affeshpaps aertiqqcce nillnaawlk
      901 rdadsihqyl lqrktsppsv
//
