LOCUS       NM_025533               2134 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus nitric oxide synthase interacting protein (Nosip),
            transcript variant 1, mRNA.
ACCESSION   NM_025533
VERSION     NM_025533.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Flach,H., Krieg,J., Hoffmeister,M., Dietmann,P., Reusch,A.,
            Wischmann,L., Kernl,B., Riegger,R., Oess,S. and Kuhl,S.J.
  TITLE     Nosip functions during vertebrate eye and cranial cartilage
            development
  JOURNAL   Dev Dyn 247 (9), 1070-1082 (2018)
   PUBMED   30055071
REFERENCE   3  (bases 1 to 2134)
  AUTHORS   Hoffmeister,M., Krieg,J., Ehrke,A., Seigfried,F.A., Wischmann,L.,
            Dietmann,P., Kuhl,S.J. and Oess,S.
  TITLE     Developmental neurogenesis in mouse and Xenopus is impaired in the
            absence of Nosip
  JOURNAL   Dev Biol 429 (1), 200-212 (2017)
   PUBMED   28663132
  REMARK    GeneRIF: Nosip is a novel factor critical for neural stem
            cell/progenitor self-renewal and neurogenesis during mouse and
            Xenopus development.
REFERENCE   4  (bases 1 to 2134)
  AUTHORS   Hoffmeister,M., Prelle,C., Kuchler,P., Kovacevic,I., Moser,M.,
            Muller-Esterl,W. and Oess,S.
  TITLE     The ubiquitin E3 ligase NOSIP modulates protein phosphatase 2A
            activity in craniofacial development
  JOURNAL   PLoS One 9 (12), e116150 (2014)
   PUBMED   25546391
  REMARK    GeneRIF: NOSIP mediates the monoubiquitination of the PP2A
            catalytic subunit and the loss of NOSIP results in an increase in
            PP2A activity in craniofacial tissue in NOSIP knockout mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2134)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DV648837.1, BC089029.1 and AC126256.4.
            
            On Jul 29, 2009 this sequence version replaced NM_025533.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076736.1, BC089029.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                DV648837.1         1-74
            75-1046             BC089029.1         1-972
            1047-2134           AC126256.4         121589-122676
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.1 cM"
     gene            1..2134
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /note="nitric oxide synthase interacting protein"
                     /db_xref="GeneID:66394"
                     /db_xref="MGI:MGI:1913644"
     exon            1..103
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /inference="alignment:Splign:2.1.0"
     exon            104..178
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..1014
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase NOSIP; RING-type E3 ubiquitin
                     transferase NOSIP"
                     /codon_start=1
                     /product="nitric oxide synthase-interacting protein
                     isoform 1"
                     /protein_id="NP_079809.1"
                     /db_xref="CCDS:CCDS21228.1"
                     /db_xref="GeneID:66394"
                     /db_xref="MGI:MGI:1913644"
                     /translation="MTRHGKNCTAGAVYTYHEKKKDTAASGYGTQNIRLSRDAVKDFD
                     CCCLSLQPCHDPVVTPDGYLYEREAILEYILHQKREIARQVKAYEKQRGARREEQKEL
                     QRAAAQDQVRGFLEKEAAIVSRPLNPFMPKAATLPNTEGEQPGPSVGPVGKDKDKALP
                     SFWIPSLTPEAKATKLEKPSRTVTCPMSGKPLRMSDLTSVRFTQLDDSVDRVGLITRS
                     ERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVNGDTLTERDIIVL
                     QRGGTGFAGSGVKLQAEMSRPVMQA"
     exon            179..284
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /inference="alignment:Splign:2.1.0"
     exon            285..366
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /inference="alignment:Splign:2.1.0"
     exon            367..526
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /inference="alignment:Splign:2.1.0"
     exon            527..645
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /inference="alignment:Splign:2.1.0"
     exon            646..833
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /inference="alignment:Splign:2.1.0"
     exon            834..942
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /inference="alignment:Splign:2.1.0"
     exon            943..2134
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1798..1803
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /note="hexamer: AATAAG"
     regulatory      1811..1816
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /note="hexamer: ATTATA"
     polyA_site      1830
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
     polyA_site      1834
                     /gene="Nosip"
                     /gene_synonym="2310061K06Rik; CGI-25"
                     /note="major polyA site"
ORIGIN      
        1 ctcctgtcgg gcggaagtag gaggagtaga gtttaaaaac agtactcttt ttccggttcg
       61 ggacgtagtt gaagcaacga caagccggat aaccgctctt gagacaggat gacgcggcat
      121 ggcaagaact gcactgcagg ggccgtctac acctaccacg agaagaagaa ggacacagcg
      181 gcctcgggct atgggaccca gaacattcga ctgagccggg acgctgtgaa agactttgac
      241 tgctgctgtc tctctctgca gccctgccat gaccctgtgg tcacgccaga cggctacctg
      301 tatgagaggg aagcgatcct ggagtacatc ctgcaccaga agagagagat cgcccggcag
      361 gtgaaggcct atgagaagca gcgaggagcg aggagagaag agcagaaaga gctgcagcga
      421 gctgcagccc aggaccaagt tagaggcttc ctagagaagg aggcggccat cgtgagccgg
      481 cccctcaacc ccttcatgcc caaggctgcc accttgccca acacagaggg tgaacagcca
      541 gggcccagtg tgggtcccgt aggcaaggac aaggacaaag cactgcccag cttctggatt
      601 ccctcactaa cgcccgaggc aaaggccacc aagctggaaa agccgtcccg cacggtgacc
      661 tgcccgatgt ccgggaagcc actgcgcatg tcggacctga cgtcagtgcg cttcacgcag
      721 ctggacgact ctgtggaccg cgtgggactc atcacacgca gtgagcgcta cgtttgcgca
      781 gtgacccgag acagtctgag caatgccacg ccgtgcgcag tgctgcgtcc ctctggggct
      841 gtggtcaccc tggagtgtgt tgagaaactg atccggaagg acatggtgga ccccgtgaat
      901 ggggacacgc tgacagagcg cgacatcatc gtgctgcagc ggggcggcac cggctttgcg
      961 ggctccggag tgaagctaca ggcagaaatg tctcggccag tgatgcaggc ctgagccgtg
     1021 gcctaataaa cgtggatgtg aacatctgga tgcctggcgc ctcattgtag gcccggcgac
     1081 agaaggagtg ggcctgcggt gtgggcctgg gctcctggga tttactcagt cacttaggac
     1141 caggcagaaa ccccggcgtg aacctgtggg tctcctccag cctcaggcag gacttggaag
     1201 agggtgccct gaacaagaag tgcaagtctc gtgatagcac tcccaggcca gctacctccc
     1261 gagaccccta gacatggacc tgcccccacc aagtgcccct cccccagcga tccctacaga
     1321 cccattacac actgcacact gcttcacatg cacgtaccct cctcctcttc tttccctgaa
     1381 cacctgagaa tatctctcaa gagccaactt cccttcccca gcccccttaa acttgctcaa
     1441 agagacgcag accaccctca tccctgcctc caagtaaaaa tagctttctt ttctctttgt
     1501 actggcaaat gcacacacta agcaagtgtt gagttcctta atgtatatct aagggagggg
     1561 tttgtgcaca tgagtccaat gcctgcatag gccgtactaa cctgtcagac ccctggctct
     1621 ggggtcaggc agccttgagt agcctagtct agatgctggg ggccaggctt gggtcctctg
     1681 ccaagagcag caagcactct aaaccactga gccatcttca ccccgtgttt ccatcccatc
     1741 acagaagaga actgctgcaa aattgaggct actttgtgct gtataagact gcctcagaat
     1801 aagagaaaca attatataca tttgagtcct taaatctggc caaattcttt gttgttttat
     1861 ttttcgagac agggtttctc tgtgtagccc tggctgtcct ggaactcact ttgtagacca
     1921 ggctggcctc gaactcagaa atccacctga ctctgctggg attaaaggcg tgcgctacca
     1981 cgcccggctg ttgggcaaat tcttgatgga aaaaaccaaa acactggcca tgcagtgatg
     2041 agaagatgtg ttcaatgtgt ggcaacttgt gtatttataa ttttacatcc tgtaagtctg
     2101 ttgctaatat ttaaacaaaa cgtatattcc aggc
//
