LOCUS       NM_008712               9695 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus nitric oxide synthase 1, neuronal (Nos1), mRNA.
ACCESSION   NM_008712 XM_992536
VERSION     NM_008712.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Zhu,Y., Meerschaert,K.A., Galvan-Pena,S., Bin,N.R., Yang,D.,
            Basu,H., Kawamoto,R., Shalaby,A., Liberles,S.D., Mathis,D.,
            Benoist,C. and Chiu,I.M.
  TITLE     A chemogenetic screen reveals that Trpv1-expressing neurons control
            regulatory T cells in the gut
  JOURNAL   Science 385 (6708), eadk1679 (2024)
   PUBMED   39088603
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Bentefour,Y. and Bakker,J.
  TITLE     Stress during pubertal development affects female sociosexual
            behavior in mice
  JOURNAL   Nat Commun 15 (1), 3610 (2024)
   PUBMED   38688927
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9695)
  AUTHORS   Chachlaki,K.
  TITLE     A role for nNOS in mediating stress and female sexual behavior in
            mice
  JOURNAL   Nat Commun 15 (1), 3609 (2024)
   PUBMED   38688905
  REMARK    GeneRIF: A role for nNOS in mediating stress and female sexual
            behavior in mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9695)
  AUTHORS   Chamberland,S., Grant,G., Machold,R., Nebet,E.R., Tian,G.,
            Stich,J., Hanani,M., Kullander,K. and Tsien,R.W.
  TITLE     Functional specialization of hippocampal somatostatin-expressing
            interneurons
  JOURNAL   Proc Natl Acad Sci U S A 121 (17), e2306382121 (2024)
   PUBMED   38640347
REFERENCE   5  (bases 1 to 9695)
  AUTHORS   Virtanen,H.T., Choopanian,P., Porokuokka,L.L., Forsgard,R.,
            Garton,D.R., Olfat,S., Korpela,R., Mirzaie,M. and Andressoo,J.O.
  TITLE     Interindividual Variation in Gut Nitrergic Neuron Density Is
            Regulated By GDNF Levels and ETV1
  JOURNAL   Cell Mol Gastroenterol Hepatol 18 (6), 101405 (2024)
   PUBMED   39299667
REFERENCE   6  (bases 1 to 9695)
  AUTHORS   Nelson,R.J., Demas,G.E., Huang,P.L., Fishman,M.C., Dawson,V.L.,
            Dawson,T.M. and Snyder,S.H.
  TITLE     Behavioural abnormalities in male mice lacking neuronal nitric
            oxide synthase
  JOURNAL   Nature 378 (6555), 383-386 (1995)
   PUBMED   7477374
REFERENCE   7  (bases 1 to 9695)
  AUTHORS   Proschel,C., Blouin,M.J., Gutowski,N.J., Ludwig,R. and Noble,M.
  TITLE     Limk1 is predominantly expressed in neural tissues and
            phosphorylates serine, threonine and tyrosine residues in vitro
  JOURNAL   Oncogene 11 (7), 1271-1281 (1995)
   PUBMED   7478547
REFERENCE   8  (bases 1 to 9695)
  AUTHORS   Morley,J.E. and Flood,J.F.
  TITLE     Effect of competitive antagonism of NO synthetase on weight and
            food intake in obese and diabetic mice
  JOURNAL   Am J Physiol 266 (1 Pt 2), R164-R168 (1994)
   PUBMED   7508208
REFERENCE   9  (bases 1 to 9695)
  AUTHORS   Huang,P.L., Dawson,T.M., Bredt,D.S., Snyder,S.H. and Fishman,M.C.
  TITLE     Targeted disruption of the neuronal nitric oxide synthase gene
  JOURNAL   Cell 75 (7), 1273-1286 (1993)
   PUBMED   7505721
REFERENCE   10 (bases 1 to 9695)
  AUTHORS   Lyons,C.R., Orloff,G.J. and Cunningham,J.M.
  TITLE     Molecular cloning and functional expression of an inducible nitric
            oxide synthase from a murine macrophage cell line
  JOURNAL   J Biol Chem 267 (9), 6370-6374 (1992)
   PUBMED   1372907
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114617.16 and AC114993.18.
            
            [WARNING] On Mar 11, 2025 this sequence was replaced by
            NM_008712.4.
            
            On May 5, 2015 this sequence version replaced NM_008712.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.7932.1,
                                           SRR10223970.7936.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1088              AC114617.16        136827-137914
            1089-1215           AC114617.16        149272-149398
            1216-1344           AC114617.16        163814-163942
            1345-1490           AC114617.16        165769-165914
            1491-1653           AC114617.16        166661-166823
            1654-1745           AC114617.16        167699-167790
            1746-1887           AC114617.16        170459-170600
            1888-2027           AC114617.16        173809-173948
            2028-2202           AC114617.16        175299-175473
            2203-2304           AC114617.16        177978-178079
            2305-2499           AC114617.16        180046-180240
            2500-2585           AC114617.16        180755-180840
            2586-2730           AC114617.16        182711-182855
            2731-2835           AC114617.16        183785-183889
            2836-2894           AC114617.16        184372-184430
            2895-3011           AC114617.16        189384-189500
            3012-3186           AC114617.16        193232-193406
            3187-3325           AC114617.16        195602-195740
            3326-3404           AC114617.16        196441-196519
            3405-3598           AC114993.18        191597-191790       c
            3599-3768           AC114993.18        189903-190072       c
            3769-3979           AC114993.18        184874-185084       c
            3980-4067           AC114993.18        184303-184390       c
            4068-4189           AC114993.18        182272-182393       c
            4190-4338           AC114993.18        180641-180789       c
            4339-4533           AC114993.18        178381-178575       c
            4534-4652           AC114993.18        175326-175444       c
            4653-9695           AC114993.18        169369-174411       c
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 57.29 cM"
     gene            1..9695
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /note="nitric oxide synthase 1, neuronal"
                     /db_xref="GeneID:18125"
                     /db_xref="MGI:MGI:97360"
     exon            1..1088
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     CDS             379..4668
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /EC_number="1.14.13.39"
                     /note="nitric oxide synthase, brain; neuronal nitric oxide
                     synthase; NOS type I; neuronal NOS; constitutive NOS;
                     peptidyl-cysteine S-nitrosylase NOS1"
                     /codon_start=1
                     /product="nitric oxide synthase 1"
                     /protein_id="NP_032738.1"
                     /db_xref="CCDS:CCDS19606.1"
                     /db_xref="GeneID:18125"
                     /db_xref="MGI:MGI:97360"
                     /translation="MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIIS
                     DLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEG
                     FTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQ
                     HAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAV
                     NRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSE
                     NEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGS
                     IMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTS
                     TYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYAT
                     NKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQ
                     GWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPA
                     VSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSL
                     WKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPP
                     MSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKF
                     SAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEH
                     EALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRK
                     SSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKM
                     REGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLT
                     YVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPG
                     DHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPC
                     TIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTM
                     VEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEG
                     PVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQ
                     RQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPK
                     KYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAG
                     VFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS"
     exon            1089..1215
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1344
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1490
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1653
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1745
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1887
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2027
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2028..2202
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2304
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2499
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2585
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2586..2730
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..2835
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2836..2894
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2895..3011
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3012..3186
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3187..3325
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3326..3404
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3405..3598
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3599..3768
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3769..3979
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3980..4067
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4068..4189
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4190..4338
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4339..4533
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4534..4652
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4653..9695
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      5219
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /note="major polyA site"
     regulatory      5805..5810
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
                     /note="hexamer: AATAAA"
     polyA_site      5827
                     /gene="Nos1"
                     /gene_synonym="2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS;
                     NO; NOS; Nos-1; NOS-I"
ORIGIN      
        1 ctccaggtag gagcccctac atcacctctg catctgacaa gctggtgacc aagatgccca
       61 gagcctagtt cccatgtttg tgcgtcacag tcatcagaca cggcaagcct ccagtcttcc
      121 tgacctgttg cttagggaca caacccatca ctgttcctga cgtctgcctg gtcaaccttg
      181 acttccttag agaataagga agggggcggg aacacatgaa atcatgccac ccaaggccga
      241 acggcaatga gcaggtgacg tcaagttgac gtcaaagaca gaggcgacag aaactctgca
      301 gccggttctt gcccggagta gctcaggttc ctgtgggagt cgtcttggct tggaggtctt
      361 ctgaaggaca cagataccat ggaagagcac acgtttgggg tccagcagat ccaacccaac
      421 gtcatttctg tccgtctctt caaacgcaaa gtgggaggtc tgggcttcct ggtgaaggaa
      481 cgggtcagca agcccccggt gatcatctca gatctgatcc gaggaggtgc cgcggagcag
      541 agcggcctta tccaagccgg cgacatcatt ctcgccgtca atgatcggcc cctggtagac
      601 ctcagctatg acagcgctct ggaagttctc aggggcattg cctctgagac ccatgtggtc
      661 ctcattctga gaggccctga gggctttact acacacctgg agaccacctt cacaggggat
      721 ggaaccccca agaccatccg tgtgacccag cccctgggga cacccaccaa agctgtcgat
      781 ctgtctcgcc agccatcagc cagcaaagac cagccattag cagtagacag ggtcccaggt
      841 cccagtaacg gacctcagca tgcccaaggc cgtggacagg gagccggctc agtctcccag
      901 gctaatggtg tggccattga ccccacaatg aaaaatacca aggccaacct ccaggacagc
      961 ggagaacagg atgaactgct caaagagata gaacctgtgt tgagcatcct caccggtggg
     1021 ggaaaagcag tcaacagagg gggaccagcc aaagcagaga tgaaagacac aggaatccag
     1081 gtggacagag acctcgacgg caaactgcac aaagctccgc ccctgggcgg ggagaatgat
     1141 cgagtcttca atgatctgtg ggggaagggc aacgtccctg tggtcctcaa caacccgtat
     1201 tcagagaacg aacagtctcc cgcctcgggc aaacagtctc ctaccaagaa tggcagtcct
     1261 tccaggtgcc ctcgcttcct taaggtcaag aactgggaga cagatgtggt cctcactgac
     1321 accctgcacc tgaagagcac gctggaaacg gggtgcacag agcagatctg catgggctcc
     1381 atcatgctgc catcccatca catacggaag tcagaagatg tccgcaccaa ggaccagctc
     1441 ttccctctag ccaaagaatt tctcgaccaa tactactcct ccattaagag gtttggctcc
     1501 aaggcccaca tggacaggct ggaggaggtg aacaaggaga tcgagagcac cagcacctac
     1561 cagctcaagg acacggagct catctatggc gccaagcatg cctggcggaa cgcctctcgc
     1621 tgtgtgggca ggatccagtg gtccaagctg caggtgttcg atgcccgaga ctgcaccaca
     1681 gcccatggga tgttcaacta catctgtaac cacgtcaagt acgccaccaa caaagggaac
     1741 ctcaggtcgg ccatcactat attccctcaa aggactgatg gcaagcatga cttccgagtg
     1801 tggaactcgc agctcatccg ctatgccggc tacaagcagc cagatggctc taccttgggc
     1861 gatccagcta atgtggagtt cacagagatc tgtatacagc agggctggaa acccccaaga
     1921 ggccggttcg atgtgctgcc tctcctgctc caggccaatg gcaatgaccc tgaactcttt
     1981 cagatccccc cagagctggt gctggaagta cccatcaggc accccaagtt cgactggttt
     2041 aaggacctgg ggctcaaatg gtatggcctc cccgctgtgt ccaacatgct gctggagatt
     2101 gggggcctgg agttcagcgc ctgtcccttt agtggctggt acatgggcac ggagattggc
     2161 gttcgtgatt actgtgacaa ctctcgatac aacatcctgg aggaagtagc caagaaaatg
     2221 gatttggaca tgaggaagac atcctccctc tggaaggacc aagccctggt ggagattaac
     2281 attgctgtcc tatacagctt ccagagtgac aaggtgacca ttgtggacca ccactctgcc
     2341 accgagtcct tcatcaaaca catggagaat gagtaccgct gcagaggggg ctgtcccgcc
     2401 gactgggtgt ggattgtgcc ccccatgtcg ggcagcatca cccctgtctt ccaccaggag
     2461 atgctcaact accggctcac cccgtccttt gagtaccagc ctgatccatg gaacacccac
     2521 gtgtggaagg gcaccaatgg gacccccacg aagcggcgag ccattggctt caagaaattg
     2581 gcagaggccg tcaagttctc agccaagctg atgggacagg ccatggccaa gagagtcaaa
     2641 gcgaccattc tctacgccac cgagacaggc aaatcccaag cctatgccaa gaccctgtgc
     2701 gagatcttca agcatgcctt cgatgccaag gctatgtcca tggaggaata tgacatcgtg
     2761 cacctggagc acgaagccct ggtcttggtg gtcaccagca cctttggcaa tggagacccc
     2821 ccagagaatg gggagaaatt cggctgtgct ttgatggaga tgaggcaccc caactctgtg
     2881 caagaggaga ggaagagcta caaggtccga ttcaacagcg tctcctccta ttctgactcc
     2941 cgcaagtcat caggcgatgg accagacctc agagacaact ttgaaagtac tggaccgctg
     3001 gccaatgtga ggttctcagt cttcggcctg ggctctcggg cataccctca cttctgtgcc
     3061 tttgggcacg cagtggacac tctcctggag gagctgggcg gggagaggat tctgaagatg
     3121 agggagggag atgagctctg cggacaggaa gaggctttca ggacctgggc taagaaagtc
     3181 ttcaaggcag cctgtgatgt gttctgtgtg ggggatgacg tcaacattga gaaggcgaac
     3241 aattccctca ttagcaatga ccgcagctgg aagaggaata aattccgcct cacttatgtg
     3301 gcagaagctc cagagctgac ccaaggtctt tccaatgttc acaaaaagcg agtctcagcc
     3361 gcccgactcc tcagccgcca aaacctgcaa agtcctaaat ccagccgatc gaccatcttc
     3421 gtgcgtctcc acaccaacgg gaatcaggag ttgcagtacc agccagggga ccacctgggt
     3481 gtcttccctg gcaaccatga ggacctcgtg aatgcactca ttgaacggct ggaggacgcg
     3541 ccgcctgcca accacgtggt gaaggtggaa atgctggagg aaaggaacac ggctctgggt
     3601 gtcatcagta attggaagga tgaatctcgc ctcccaccct gcaccatctt ccaggccttc
     3661 aagtactacc tggacatcac cacgccgccc acgcccctgc agctgcagca gttcgcctcc
     3721 ctggccacca atgagaaaga gaagcagcgg ctgctggtcc tcagcaaggg gcttcaggaa
     3781 tatgaggaat ggaaatgggg caagaacccc acaatggtgg aggtgctgga ggagttcccg
     3841 tccatccaga tgccggccac acttctcctc acacagctgt cgctgctaca acctcgctac
     3901 tattccatca gctcctctcc agacatgtac cccgacgagg tgcacctcac cgtggccatc
     3961 gtgtcctacc atacccgaga tggagaagga ccggttcacc acggggtgtg ctcctcctgg
     4021 ctcaaccgaa tacaggctga cgatgtcgtc ccctgcttcg tgagaggtgc ccctagcttc
     4081 cacctgcctc gaaaccccca ggtgccttgc atcctggttg gcccaggcac cggcatcgct
     4141 cccttccgaa gtttttggca acagcgacaa tttgacatcc aacacaaagg aatgaatccg
     4201 tgccccatgg ttctggtctt cgggtgtcga caatccaaga tagatcatat ctacagagag
     4261 gagaccctgc aggccaagaa caagggcgtc ttcagagagc tgtacactgc ctactcccgg
     4321 gaacccgaca ggccaaagaa atatgtacag gacgtgctgc aggaacagct ggccgagtct
     4381 gtgtaccgcg ccctgaagga gcaaggaggc catatttatg tctgcgggga cgttaccatg
     4441 gccgctgatg tcctcaaagc catccagcgc ataatgaccc agcaggggaa actctcggag
     4501 gaggatgctg gtgtgttcat cagcaggctg agggatgaca accggtacca cgaggacatc
     4561 tttggggtca ccctcagaac atatgaagtg accaaccgcc ttagatctga gtccatcgcc
     4621 ttcattgaag agagcaaaaa agacacagat gaggttttca gctcctaact ggatcctccc
     4681 gccccatggg atatggggtg gctgccctga gtgcccacgc gagggcggcc gcaagttgac
     4741 taaacgggga cacacacggc tgaaccttcc ccacgggacc ccgagtggcc ccaccctgcc
     4801 tcttgtcctg ttgctatgtc ctggtccttc ttcccgctct gggcttctct caaccccaac
     4861 cctgggtttt ctcctagact cttgggctgc gatgcatcac ccttgttacc ctgcagtggc
     4921 tcttacaaaa ccacttcctc cccacccgct tgctgccaag gggcgggttg cggtgcatga
     4981 aaccgctggg tcctggctgt cactcctgtt gggggtctga aggtggctgg cactgggcct
     5041 caggtcaccc tgagccagtc ccgtggccac ttcagccctt ttccatcctc tttttatgac
     5101 ggtgtgtgtg agagagtggt ggggcggggt gagaaattct ctgtcctttt gtctacacat
     5161 gcgtgctgac cttacctgtg agtccactgc cttcgtgaag gatgctccga gccatgaaac
     5221 atgttcctag atgtcgttaa tcccacccac gtccaccatg acaggcctgt gtcagcctcc
     5281 ctgaaacaca gcagcccccc ttactaatca caggcacaaa tggagaggaa ggggtccctg
     5341 tggaggaccc aaccacagaa tctgaggtgt tcagaatctg accctcttgg cagagatctg
     5401 tctgggaaat ttaaccaggc agtggatcag ccaagcccct ccccattctc accaacccca
     5461 gctgggacca caggatgttg agctgtcttc cccaggccca gcagccatgc cccgccaaga
     5521 agcatgctgg gagtaaggtc tttgccatca agggtatttg ggtgacattt caaaatttgt
     5581 cctcattaaa tggatgggtg ggccttttta gtgctttgga gccttggcag atgtcaggga
     5641 ggaatagggt agctgctttt cattcctcct gcttgacaat aattcccttc ccccaaaatg
     5701 agcagtgtag gcaagaaaat tctagccatg accagtggtc cctcagagct aaccatctct
     5761 gaggcattgt ggtggtccct tgtctgataa ataaataatg aataaataaa agttatatca
     5821 gacagtactg acagacaata aagaaacccc ccccccaggg tgcccaggtt aagtcccatg
     5881 ttctgtgctc ctgtttggtt ttgggaggca ggaaaaaatc aaaacacaac acaaccattt
     5941 cctctatgtc ctgagctggg acctgctgtc ctaatgctaa ggatgagggc tgtcaaacag
     6001 aaaatgctga agactttaca gctgtggggt ccaaaaccct aagggctcca atcccaccca
     6061 cattctttct cggtctttgt ctctctttct tcctttcctt tctatttccc ccagggtctc
     6121 ctgttaacca ggctgacttc gaactcacaa cctttaattt ctaaccctac cacatcacat
     6181 ccagtttatg atttttccag aggctcaaag ccagaacctc ctgcatgttg gacaagcact
     6241 ctaccaactg agctacatcc ccagccttca gtccccaccc ccgagtccag agacaggtct
     6301 catgtgacac tggacaaccc tgccatcact agcatgtgaa cctggtccta gttggaaact
     6361 cacccagcct accttagtac tagtgtttcc tacttgatct cccatgcctg tggagactaa
     6421 gcccccacta ccccagagct atcttctgta gcccaaaggc aaggcagtga cctcctactg
     6481 acttccaaca caatccaggg acattcttct caacccttca aacttctggt gacacttaag
     6541 atgtagctga ttcccctcat ccgaactgct gtgtccaacc tccattgaag agccttttct
     6601 ccctccctct ccctctgcaa gccaagctgc atgcacccat gcatgagtgg ttcttgcacc
     6661 cctgaatgag gaatgcttcc tctgtgacag gctcctcccc acccccggat atcccggtgc
     6721 tgccagtagt gagtggacag atggaagaag caccgtgtca tttgccagag tacaaagaca
     6781 cgattacata accaggttca agggggactt ctgcttatca gtgctaggga tgggacccag
     6841 gacctcaccc acgccaggct gagctatgtc cccaaccccc aagacagaat attttaaaaa
     6901 cacatttgaa ctcttgacac ttccagctcc ctcagctcct ggctagaagg ctggagatca
     6961 gttggatatc ctctctcctt tttgaaaacg cccctaagta gtttatccaa aaaaaaaaaa
     7021 aaaaaacgaa gcaaggtggc catctggggt gagtcgatac tgccctctgc tggttgtttc
     7081 tagttcaagc ccacagccag caacaagcaa caaagtgtct tagaatagga ctggttttgt
     7141 ttacctcacc tatgaggttg gcagtagctc ccaacccatt tcacaagagg aggtttgacc
     7201 attacgctct cagctattcc tgcagccatg gtccccaaga agtttagcct atgggagaag
     7261 agagggtttc cctacatatc cccagagaaa tcaggtgact ttttcccgga gatgggggtg
     7321 ggggatgttt aggtcccact atgatgcaag cagccatgac ccacattctg ttgctgcctc
     7381 gtttatgcag aaggtctgat tccagatcgt acctaagcaa gacaatgctc caagactgta
     7441 ccaagccaag ctctgataag cttccatctt ccatggctgg tagactgaac tgtcccagaa
     7501 tcactagctt gaggtagagg aggggagggc agagtcacgg attcttaagc taagtgggca
     7561 agacctggta ccgggtctgt ggtagagagt aaagaccaag ggagtagaga gatcccagcc
     7621 ctgggaggag aaggaagttg ggtcaggcct cggtacaaag ggaaaacact gaattggccc
     7681 caaaacacag gagcaagcgt gtggatttcc attgcagagc ccctgtttca ggatgcaaga
     7741 gttttccagc ccaaggcaaa atggaagatt gctgacccaa ccacaaaccc atccctgcgg
     7801 agtctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc
     7861 tctctctctc tctcacacac acacacacac tcacacacac acacacacac acacacatac
     7921 acacacagcc tttcactgga agccatgggc ttccaggtta agttcagcca agggacccaa
     7981 tgcagacatc cttcgtggac agggttcagc cttgggaggc caggctggct ggctcactct
     8041 ctttctcatt cactgccaga ccccacatgg agaggcaccc aaaaggagaa gttgaccatt
     8101 ccatgtctca agtatgagga cccagggacc ccagggaatc tgttttctat ttacaagtct
     8161 gacatcttaa tccccactgc caaaatgctc cgaaatctaa aacgtgttga gcaccaacag
     8221 gataccacaa gtaaaaactt tagttagttc atctacaaaa gtattcaaaa gaatacatag
     8281 actggacacg aagctcaaag gtagagttcg cctagcatgc atacgaagcc tggatcccat
     8341 atcccagcag ggcaaaccaa ttgcctcagg ctatctgtgt aaggtgtcta tgtgaaacct
     8401 aaatgaattt catgattaga ggtgctccca cctgccaaat aatcccgcca caaatatgca
     8461 aatattgcaa aatcaaaaat aattctgaaa ttattctgaa acacttcttg tcacaagaat
     8521 ttcagcaaag tacagtcaac ctatcagaga gacaagggtg atgccccggt gagtgtgtct
     8581 cagctgtgtt tatgaatttg tttggttcac acatatctga gtggtatgga taactgggca
     8641 tctctaaccc agggcaatca gtaagaacta ataacctgtg tctcctagac gagtcaggat
     8701 gggaactgac ctctaggaga ttctagtcag gagcccacta acctgggccg tcatgtttag
     8761 agctgaagga gtcttcatag cccaggctgt cctgggtatt gcaggatgct gattgaacag
     8821 cacctgtgac ctttcctgct ccatgccact agcaacctcc tgccatgtgt ggcagtcaca
     8881 aaggtcatca gaatggagtg agtgctccct gggaaggcag gcaggacctt caaccattgg
     8941 accaagaagt cattgcttat gggtctcagg ggccatcaaa ctgtctacct gatgctgtgg
     9001 tttctgctta gagatctgga ggagggcagc ggagcctgcc tcggttcagt gatggttttt
     9061 cttacctgct tcaagccgag atctgaaagc tttgtggaag atgatcaagc aaagctccca
     9121 agccaaaggg tagcagaggg tggctacgtg gtttttaggt agatgttccc aggtgccctc
     9181 tcttgagctg ggtctgaggt tatccctgtc cccatgcccc ctctgtgccc cgagctctgt
     9241 ctccccactc ttgctgttgt gttgtccttg tgaatgtatg gctgacccac tccaggccaa
     9301 gcagcttaac agcaggcttg ctgttaagtc ccaagtccgt catggggtca ggttacaccc
     9361 agaggtgttt tcactgtggg gttccaggga ggctggggag aatgggcaga gcccagggtt
     9421 ccatctcacc acctataagt tgtcagaaac tcggatgccc ctccacccac ctctggcatc
     9481 tctatgctgc taatctctgc cacgtgtccc gtgtgctcca gtgcccgtgc ccgtcaccgc
     9541 ccctccacgc cctccgtgag gttgtccagg ctgtgttctc tcgcatgctt ctcttctata
     9601 taaccctcat cttctcattt tatgggattc aacacaaagt ctacttatgc ttctttgcat
     9661 tatggttaaa caattaaaaa aaaaaaaagg actca
//
