LOCUS       NM_001271397            4655 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus nucleolar protein 8 (Nol8), transcript variant 1,
            mRNA.
ACCESSION   NM_001271397 NM_027607
VERSION     NM_001271397.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 4655)
  AUTHORS   Tarantino,C., Paolella,G., Cozzuto,L., Minopoli,G., Pastore,L.,
            Parisi,S. and Russo,T.
  TITLE     miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic
            stem cells
  JOURNAL   FASEB J 24 (9), 3255-3263 (2010)
   PUBMED   20439489
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK014994.1, BC125575.1 and AK147172.1.
            
            On Sep 20, 2012 this sequence version replaced NM_027607.1.
            
            Transcript Variant: This variant (1) encodes the protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC125575.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-274               AK014994.1         2-275
            275-4369            BC125575.1         1-4095
            4370-4655           AK147172.1         4106-4391
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 25.36 cM"
     gene            1..4655
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /note="nucleolar protein 8"
                     /db_xref="GeneID:70930"
                     /db_xref="MGI:MGI:1918180"
     exon            1..605
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             569..4066
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /codon_start=1
                     /product="nucleolar protein 8"
                     /protein_id="NP_001258326.1"
                     /db_xref="CCDS:CCDS36658.1"
                     /db_xref="GeneID:70930"
                     /db_xref="MGI:MGI:1918180"
                     /translation="MERPLENVSSNLDRCPTSMQGNREMKRLFVGGLGQGISETDLQN
                     QFGRFGEVSDVEIITRKDDQGNSQKVFAYVNIQITEADLKKCMSILNKTKWKGGTLQI
                     QLAKESFLHRLAQEREDAKAKKEKSTTGNPTLLEKMGAVDFHMKAVPGTEVPGHKNWV
                     VSKFGRVLPVLHLKNQQKHKIMKYDPSKYCHNIKKIPENLTETTPIAELTWELEGGND
                     PMSKKRRGEFSDFHIPPQKVKKVQKSNDPMESKVSNIGLRTNQVMEKNKSTHPVTAHG
                     TAPSTVNPSKQLLVSSSGTQKPKHVVFHNSDFEIIWNKSSMSDDDVDSEDELKMMIAK
                     EENREKPGHSSVNESEHDTFEVVRDDFKSNIHKLSSSVSLGNNHEYDSSDTDEIIAMK
                     TKNAKVKNSAESSQPERTVSKKSSFQKIEPSNDCIKVQGINSNKESALCHGVKFVNPK
                     FPPDSSGSDSEESEEDEEYKVLMENCPRVSLTLADLEQLAGSHRKFPGKDSETNGPQN
                     DSHCKFDTTSKNPKTSGDLYNGRQQCILPEEIVASLLEDENTYSKQKSEEDILKPKFQ
                     AFKGIGCLYAKESVDKTLKENIAFNTGGGHHSSLKHEDHNRSLMENGSKCVNGSSSKL
                     TSCQPAKKVNDPNHIQPPKRQCTFENQNHKVMSSTSCDKGSTNPLPGPLPLKAKTSLH
                     LSANSHKVDSDGDACHWPESRKALEKERTNLSNLESLEKSSKVSPREDPQKSPAGFSL
                     SDSNASCINAKDKQAEDNQKRLAALAAWQKAREVQKKLVHSALANLDGHPEDKKTHIV
                     FASDNESETEETSTQEQSCPEKELMKESVSKSPGKLFDSSDDEDSDSKEDSTRFSIKP
                     QFEGRAGQKLMDLQSQFGSDERFRMDSRFLESDSEDEKKELNEDKVNEDELAAEKKKT
                     LNVVQSVLNINVNNPTNKGSVAAKKFKDIVHYDPTKHDHAIYERKQEDKEKESKATRK
                     KKKEEAEKLPEVSQDMYYNIAADLKEIFQSMSNTDEKEEDVPRTEAGAREGTGKIRNA
                     ETLACEPEQTTGFTFSFFDSATKDEKDATYRIELVKHGKIVCPNDPRFQDSSSEEEDI
                     AEEADHSKPSPGEAVPENEAIRFFFFSENDDRLRGSNLFWSGMGGSISRNSWEARTSS
                     LLLECRKKHKEAKRKVKAN"
     exon            606..761
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            762..824
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            825..903
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1039
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1108
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..2926
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3040
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3041..3163
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3164..3254
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3255..3390
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3391..3468
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3469..3740
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3741..3867
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3868..3935
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3936..4015
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4016..4646
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4592..4597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4609
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /note="major polyA site"
     regulatory      4626..4631
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4646
                     /gene="Nol8"
                     /gene_synonym="4921532D18Rik; 5730412B09Rik"
ORIGIN      
        1 acttccgcct acttgaaccg gaaatcagga accacgcggc ggagtgtctt ttctgtagga
       61 attaccgatt tttatttttc tttctcacac tctgtcagtt ttacaacgtg gaggagaact
      121 aacagaacgt cagtaaaagc gaaggcggag gaaataaaga gccctctgga actgcgggat
      181 aacggaacct taggagattg ggttggaaac tttgtaggaa ggaaaggaaa atgcacttgc
      241 cggcacgtgt tattagacgt taagtgctac gccgggcttg agctgagtcg gacaagcgcc
      301 ctgaggaaga gtgtttgctc gtgagaagtg atgggctatt gggcttagcc tctcctctcc
      361 ttccttaggg gcttctcgaa gagagagggc agggctggaa ggatggggcg gtggctacca
      421 agaaggggta cactgtaggt ttttttccca gagtgctggc ctttgctttg ggcttctgcc
      481 gctatttagt gatctcgctt ggaaggagac agccaacttt tgagaaaggt ttgagtaaga
      541 gctgaacatc cgccgagagc taaacgagat ggagcgacct ctggaaaacg tttccagcaa
      601 tcttgataga tgcccaacat ccatgcaagg caacagagaa atgaagcgcc tctttgtggg
      661 tggacttgga cagggcattt ctgagacaga cctacaaaat cagttcggca gatttggaga
      721 agtttctgac gtggagatca tcactcggaa agatgaccaa ggaaattcac agaaagtctt
      781 tgcgtatgtt aacattcaaa taacagaagc ggacctgaag aaatgtatgt ctattttaaa
      841 caaaacaaaa tggaaaggtg gaacactaca aattcagcta gcaaaagaaa gctttttaca
      901 cagattggcc caagaaagag aagacgcaaa agcaaagaaa gaaaaatcaa caacaggcaa
      961 ccccacctta ttggaaaaga tgggagcagt ggatttccat atgaaggctg tgcctggaac
     1021 agaagtacca gggcataaaa attgggttgt gagtaagttt ggaagagtct tacctgttct
     1081 tcaccttaag aatcaacaaa aacataaaat tatgaaatat gatccatcaa aatactgcca
     1141 caatataaaa aagattccag agaatttgac agaaaccact cccatagctg aactcacttg
     1201 ggaattggaa ggaggcaatg accctatgag taagaaacgt cgaggagagt tctctgactt
     1261 tcatatccct cctcaaaagg tcaagaaagt gcagaagagc aatgatccca tggagtccaa
     1321 ggtttctaat attggtctaa ggactaatca ggtaatggag aagaacaaat caacacatcc
     1381 tgtgactgca cacgggacag cccctagtac tgttaatcct tctaaacaac tccttgtgtc
     1441 cagttctggt actcagaaac ctaaacatgt ggtttttcat aattctgact ttgaaattat
     1501 ctggaataaa agtagcatgt ctgatgatga tgttgactcg gaagatgaat taaaaatgat
     1561 gattgcaaaa gaggaaaaca gagagaaacc tgggcattcc tcagtcaatg agtctgaaca
     1621 tgatactttt gaagttgtta gggatgattt caaatcaaat attcacaagc tttcttcttc
     1681 agtaagccta ggaaataacc atgaatatga ttcaagtgat acagatgaaa ttattgcaat
     1741 gaaaacaaaa aatgctaagg tcaaaaacag tgcagaatct tcacaaccag aaaggactgt
     1801 aagcaagaaa agttcttttc aaaagataga accttctaat gactgtataa aagtacaagg
     1861 aattaacagc aacaaagaat cagccctgtg tcatggagta aagtttgtaa atcccaaatt
     1921 tccacctgac tccagtggca gtgacagtga agagtccgaa gaagatgaag aatataaagt
     1981 cttgatggaa aactgtcccc gtgtgagtct cactttggct gacttggagc agttggctgg
     2041 cagtcatcgg aagtttccag gaaaagacag tgagactaat ggtccacaaa atgacagtca
     2101 ctgcaaattt gacacaacct ccaagaatcc caagacttct ggtgacctgt acaatggcag
     2161 acaacagtgc attcttcctg aggagattgt ggcctccctt ttagaggacg agaatactta
     2221 cagcaaacaa aaatcggaag aagatatctt aaagccaaaa ttccaggcct tcaagggaat
     2281 aggctgtctc tatgcaaagg agtcagtaga caaaactttg aaagagaata ttgcctttaa
     2341 tactggcggt gggcatcaca gttccttaaa acatgaagat cacaatagaa gtttgatgga
     2401 aaatggatct aagtgtgtta atggctcatc aagcaaactg acttcatgcc aacctgcaaa
     2461 gaaggtgaat gacccgaacc acattcagcc tccaaaaaga cagtgtactt ttgagaacca
     2521 gaaccataaa gtaatgtcct ctactagttg tgacaaggga agtacaaatc ctctcccagg
     2581 tccgttgcca ttgaaagcta aaacatccct gcatcttagt gccaacagtc acaaggtaga
     2641 ctctgatgga gacgcttgcc attggcctga aagtagaaaa gctttagaga aagagaggac
     2701 caatttaagc aacctcgaat ctcttgagaa atcatcaaag gtgtctccca gggaagaccc
     2761 tcagaaaagc ccagctggtt tctcactttc tgatagtaat gcatcctgta taaatgctaa
     2821 ggataaacaa gctgaagaca accagaagcg attggccgcc ttggcagcat ggcagaaagc
     2881 cagggaagta cagaaaaaac tggtgcatag tgccctggca aatttggatg gtcatccaga
     2941 ggacaaaaaa acacacattg tctttgcttc tgataatgaa agtgaaacgg aagagacatc
     3001 cactcaggag caaagctgtc cagaaaagga gctgatgaaa gaatctgtga gtaaatcgcc
     3061 tgggaagctg tttgacagca gtgatgatga agactcagat tccaaggagg acagcaccag
     3121 gttcagcatt aaacctcagt ttgagggcag agctggccag aagcttatgg atctgcagtc
     3181 tcagtttgga agtgatgaga gattccgcat ggactctaga tttttagaga gtgacagtga
     3241 ggacgaaaag aaagagctaa atgaagataa agtgaatgaa gacgagcttg ctgcagaaaa
     3301 aaagaaaacc ctgaatgttg tgcaaagtgt tttgaacatc aatgtgaaca acccgacgaa
     3361 caaaggatca gtggctgcta agaaatttaa ggatatcgta cattatgacc caacaaagca
     3421 tgaccatgcc atttatgaaa gaaaacaaga agataaagaa aaagaaagta aggcaacacg
     3481 gaagaagaaa aaggaagaag cagagaagct gcctgaggta tcccaagaca tgtattataa
     3541 cattgctgca gatttgaaag aaatattcca aagtatgagc aacacagatg aaaaggaaga
     3601 agatgtgccc aggactgagg ctggtgctag agaggggact ggaaaaatca ggaacgccga
     3661 gacactggca tgtgagcccg agcaaaccac tggctttaca ttctcttttt ttgattcagc
     3721 cactaaagat gaaaaggatg ccacctatag aatcgaacta gtgaaacatg ggaagatagt
     3781 ctgtccaaat gatccccgat tccaagacag tagttcagaa gaagaagata ttgctgaaga
     3841 agcagatcat agcaagccaa gccctggaga agcggttcct gagaatgagg ccattagatt
     3901 tttctttttc tctgagaatg acgacagact tcgtggttct aacttattct ggagtggtat
     3961 gggaggtagt attagcagaa attcttggga agcccgaaca agtagtctcc ttttggaatg
     4021 tcggaagaaa cataaagaag ccaaaaggaa ggtgaaagca aattaaccat tgtcagcttt
     4081 ggttttggaa ttgaatgtgc agactgctca ccagcccttg aaagaaatta acccagaaaa
     4141 gcattcactg acaaagaaga tatttcatag tagaaaaata ttcaaacctg gatgataatg
     4201 tatcattctg atgactattt ctgtggaatt cgtatacatt ccctccttga tgactgtgtt
     4261 aaaattaact gagtcagttg tgacacacag tatagcctca gcactttaga aggggaagtg
     4321 ggaggatcat ttgagcccag aatttgagat cacactgagc aacaccccac ctcagaacag
     4381 catccattct tgttcttcac tcctgacagg aggcttacat gaaaccttgt ttaataaatg
     4441 gtttgatgta tttgtttaag tatttctgac tatgctaaat attctatacc tctaatttat
     4501 ctttttaaac tcaagtacaa attaactttg agttctgtat atacagatgt aagtttctga
     4561 tgtaatttct gaagacaaaa tagaactttc taataaatgg ttcataatat ttgtgtttat
     4621 tcctaattaa atactcttta ccttcaaaaa aaaaa
//
