LOCUS       NM_013611               2095 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus nodal growth differentiation factor (Nodal), mRNA.
ACCESSION   NM_013611
VERSION     NM_013611.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Matsuoka,R., Kitajima,K., Nii,T., Zou,Z., Tanaka,K., Joo,K.,
            Ohkawa,Y., Ohga,S. and Meno,C.
  TITLE     Hyperglycaemia induces diet-dependent defects of the left-right
            axis by lowering intracellular pH
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1871 (1), 167550 (2025)
   PUBMED   39442590
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Chen,X., Su,Q., Ling,X., Yang,Y., Liu,Y., Zhu,X., He,A., Wu,H. and
            Qi,Y.
  TITLE     SENP3-regulated Nodal signaling plays a potential role in cardiac
            left-right asymmetry development
  JOURNAL   Int J Biol Macromol 274 (Pt 2), 133294 (2024)
   PUBMED   38925188
REFERENCE   3  (bases 1 to 2095)
  AUTHORS   Kim,J.H., Kim,K., Kim,I., Seong,S., Koh,J.T. and Kim,N.
  TITLE     Nodal negatively regulates osteoclast differentiation by inducing
            STAT1 phosphorylation
  JOURNAL   J Cell Physiol 239 (6), e31268 (2024)
   PUBMED   38577903
  REMARK    GeneRIF: Nodal negatively regulates osteoclast differentiation by
            inducing STAT1 phosphorylation.
REFERENCE   4  (bases 1 to 2095)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 2095)
  AUTHORS   Yull,S., Shafiei,S., Park,C.B., Kazemi,P., Tiemann,E.B., Page,M.G.
            and Dufort,D.
  TITLE     Uterine Nodal expression supports maternal immunotolerance and
            establishment of the FOXP3+ regulatory T cell population during the
            preimplantation period
  JOURNAL   Front Immunol 14, 1276979 (2023)
   PUBMED   38022561
  REMARK    GeneRIF: Uterine Nodal expression supports maternal immunotolerance
            and establishment of the FOXP3[+] regulatory T cell population
            during the preimplantation period.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2095)
  AUTHORS   Sampath,K. and Robertson,E.J.
  TITLE     Keeping a lid on nodal: transcriptional and translational
            repression of nodal signalling
  JOURNAL   Open Biol 6 (1), 150200 (2016)
   PUBMED   26791244
  REMARK    Review article
REFERENCE   7  (bases 1 to 2095)
  AUTHORS   Cereghini,S., Ott,M.O., Power,S. and Maury,M.
  TITLE     Expression patterns of vHNF1 and HNF1 homeoproteins in early
            postimplantation embryos suggest distinct and sequential
            developmental roles
  JOURNAL   Development 116 (3), 783-797 (1992)
   PUBMED   1363228
REFERENCE   8  (bases 1 to 2095)
  AUTHORS   Iannaccone,P.M., Zhou,X., Khokha,M., Boucher,D. and Kuehn,M.R.
  TITLE     Insertional mutation of a gene involved in growth regulation of the
            early mouse embryo
  JOURNAL   Dev Dyn 194 (3), 198-208 (1992)
   PUBMED   1467556
REFERENCE   9  (bases 1 to 2095)
  AUTHORS   Conlon,F.L., Barth,K.S. and Robertson,E.J.
  TITLE     A novel retrovirally induced embryonic lethal mutation in the
            mouse: assessment of the developmental fate of embryonic stem cells
            homozygous for the 413.d proviral integration
  JOURNAL   Development 111 (4), 969-981 (1991)
   PUBMED   1879365
REFERENCE   10 (bases 1 to 2095)
  AUTHORS   Robertson,E., Bradley,A., Kuehn,M. and Evans,M.
  TITLE     Germ-line transmission of genes introduced into cultured
            pluripotential cells by retroviral vector
  JOURNAL   Nature 323 (6087), 445-448 (1986)
   PUBMED   3762693
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC122197.4 and AK049290.1.
            
            On Aug 4, 2016 this sequence version replaced NM_013611.4.
            
            Summary: This gene encodes a secreted ligand of the TGF-beta
            (transforming growth factor-beta) superfamily of proteins. Ligands
            of this family bind various TGF-beta receptors leading to
            recruitment and activation of SMAD family transcription factors
            that regulate gene expression. The encoded preproprotein is
            proteolytically processed to generate the mature protein, which
            regulates early embryonic development. Homozygous knockout mice for
            this gene exhibit early embryonic lethality, while expression of a
            hypomorphic allele results in defects in anteroposterior and
            left-right patterning. [provided by RefSeq, Aug 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X70514.1, AK082823.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-316               AC122197.4         65101-65416         c
            317-2092            AK049290.1         1-1776
            2093-2095           AC122197.4         58050-58052         c
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 32.21 cM"
     gene            1..2095
                     /gene="Nodal"
                     /gene_synonym="Tg.413d"
                     /note="nodal growth differentiation factor"
                     /db_xref="GeneID:18119"
                     /db_xref="MGI:MGI:97359"
     exon            1..524
                     /gene="Nodal"
                     /gene_synonym="Tg.413d"
                     /inference="alignment:Splign:2.1.0"
     CDS             329..1393
                     /gene="Nodal"
                     /gene_synonym="Tg.413d"
                     /codon_start=1
                     /product="nodal preproprotein"
                     /protein_id="NP_038639.2"
                     /db_xref="CCDS:CCDS23878.1"
                     /db_xref="GeneID:18119"
                     /db_xref="MGI:MGI:97359"
                     /translation="MSAHSLRILLLQACWALLHPRAPTAAALPLWTRGQPSSPSPLAY
                     MLSLYRDPLPRADIIRSLQAQDVDVTGQNWTFTFDFSFLSQEEDLVWAELRLQLPGPM
                     DIPTEGPLTIDIFHQAKGDPERDPADCLERIWMETFTVIPSQVTFASGSTVLEVTKPL
                     SKWLKDPRALEKQVSSRAEKCWHQPYTPPVPVASTNVLMLYSNRPQEQRQLGGATLLW
                     EAESSWRAQEGQLSVERGGWGRRQRRHHLPDRSQLCRRVKFQVDFNLIGWGSWIIYPK
                     QYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVD
                     NGRVLLEHHKDMIVEECGCL"
     exon            525..1240
                     /gene="Nodal"
                     /gene_synonym="Tg.413d"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..2095
                     /gene="Nodal"
                     /gene_synonym="Tg.413d"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2074..2079
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nodal"
                     /gene_synonym="Tg.413d"
                     /note="hexamer: ATTAAA"
     polyA_site      2095
                     /gene="Nodal"
                     /gene_synonym="Tg.413d"
                     /note="major polyA site"
ORIGIN      
        1 aagcttcact cggagcaagc cttagcccgc tgtctcagca gggagacttc ccgaggtaga
       61 ggggcaaggt gcggggcggt ttagactcag agtctgtatg cacccctaac tccccccccc
      121 cccccccgcc acaatttctc tgtagtcttt ctcagcacat cacacctccc ctcagcaggg
      181 gctcccctgc cctgcccctc cagggtggtt ataagttctt aacctatagg ttataggcct
      241 ctccggaggg agggagggaa aggggcgggg cggcggcggc tcagatataa gggaccctgg
      301 tgttgtttgc gggtccaaac agcccaccat gagtgcccac agcctccgca tccttcttct
      361 tcaagcctgt tgggctctac tccacccgcg cgccccgacc gcggccgctt tgcctctgtg
      421 gacacggggg cagccctcgt caccgtcccc tctggcgtac atgttgagcc tctaccgaga
      481 cccgctgcct cgggcggaca tcatccgcag cctccaggcg caagatgtgg acgtgaccgg
      541 acagaactgg actttcacgt ttgacttctc ttttttgagc caagaagagg atctggtatg
      601 ggcggagctc cggttgcagc tgccgggccc catggacata cccactgagg gcccactcac
      661 cattgacatt ttccaccagg ccaaggggga tccagagcgg gaccccgctg actgcctgga
      721 gcgcatttgg atggagacgt tcaccgtcat tccttctcag gtcacgtttg cctcaggcag
      781 cacagtcctg gaggtgacca agccactctc caagtggcta aaggacccca gggcactgga
      841 aaagcaggtg tccagtcgag cagaaaagtg ttggcatcag ccctacaccc cacctgtacc
      901 tgtcgccagc accaatgtgc tcatgctcta ctccaaccgg cctcaggagc agaggcagct
      961 agggggcgcc actttgcttt gggaagctga gagctcctgg cgggcccagg agggacagct
     1021 gtctgtagag aggggcggat ggggcagaag gcaacgccga catcatttgc cagacagaag
     1081 ccaactgtgt aggagggtca agttccaggt ggacttcaac ctgattggct ggggctcctg
     1141 gatcatctac cccaagcagt acaatgccta tcgctgtgag ggcgagtgtc ctaaccctgt
     1201 gggggaggag tttcatccta ccaaccatgc ctacatccag agcctgctga aacgatacca
     1261 accccaccgg gttccttcca cgtgctgtgc ccccgtgaag accaagccac tgagcatgct
     1321 ttatgtggac aatggcaggg tcctcctgga acaccacaag gacatgattg tggaggagtg
     1381 tgggtgcctc tgacagagcc agggggagtg ctgaaattgg cttgcattcc acaatgctga
     1441 tgaactccaa ggagactcca ttgtgtctat ccagggagca gaaacgttag aagagttctg
     1501 cctgctggag ctaaagagaa aagccccgcc ccctgtgcat acagtgctct tagacctgcc
     1561 aagccagaga gaggctaccg tggcatggca ggatggggaa gccttgcagg ggctggctcg
     1621 ctgggctccc tggaaatagg gtttatgaac tgcttgaaat tgtgtgcaaa ggctggggtg
     1681 tatatatata tatgtatata tatatatatg tatttatttt tgatgccatc tgtaactttc
     1741 ttgtattctc aaaagtggtc tgtgacctgc tgtccctccc tcaagattag tatatatttt
     1801 attagattat aaacgagcca tttggttctc cctgcctcaa gctgtggtag ggaagaccca
     1861 caaccttctg gctggctggc agtgacatac tggccttggt caggggctct ctgatctcta
     1921 atgacttgcc taaaaaagcc actgtccagt tctccagggc cagttggtgc ctttgaccag
     1981 agaggtgggc acttgtccaa gaggggactg gccatggtgg actttagaag ccagagtcct
     2041 gagatgtatg cttggcagac acaacccaag tctattaaaa gtctgtgaca attca
//
