LOCUS       NM_175460               4650 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus nicotinamide nucleotide adenylyltransferase 2
            (Nmnat2), transcript variant 1, mRNA.
ACCESSION   NM_175460
VERSION     NM_175460.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Yang,S., Niou,Z.X., Enriquez,A., LaMar,J., Huang,J.Y., Ling,K.,
            Jafar-Nejad,P., Gilley,J., Coleman,M.P., Tennessen,J.M.,
            Rangaraju,V. and Lu,H.C.
  TITLE     NMNAT2 supports vesicular glycolysis via NAD homeostasis to fuel
            fast axonal transport
  JOURNAL   Mol Neurodegener 19 (1), 13 (2024)
   PUBMED   38282024
  REMARK    GeneRIF: NMNAT2 supports vesicular glycolysis via NAD homeostasis
            to fuel fast axonal transport.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4650)
  AUTHORS   Kuribayashi,H., Katahira,M., Aihara,M., Suzuki,Y. and Watanabe,S.
  TITLE     Loss-of-function approach using mouse retinal explants showed
            pivotal roles of Nmnat2 in early and middle stages of retinal
            development
  JOURNAL   Mol Biol Cell 34 (1), ar4 (2023)
   PUBMED   36322391
  REMARK    GeneRIF: Loss-of-function approach using mouse retinal explants
            showed pivotal roles of Nmnat2 in early and middle stages of
            retinal development.
REFERENCE   4  (bases 1 to 4650)
  AUTHORS   Dingwall,C.B., Strickland,A., Yum,S.W., Yim,A.K., Zhu,J.,
            Wang,P.L., Yamada,Y., Schmidt,R.E., Sasaki,Y., Bloom,A.J.,
            DiAntonio,A. and Milbrandt,J.
  TITLE     Macrophage depletion blocks congenital SARM1-dependent neuropathy
  JOURNAL   J Clin Invest 132 (23), e159800 (2022)
   PUBMED   36287209
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4650)
  AUTHORS   Fang,F., Zhuang,P., Feng,X., Liu,P., Liu,D., Huang,H., Li,L.,
            Chen,W., Liu,L., Sun,Y., Jiang,H., Ye,J. and Hu,Y.
  TITLE     NMNAT2 is downregulated in glaucomatous RGCs, and RGC-specific gene
            therapy rescues neurodegeneration and visual function
  JOURNAL   Mol Ther 30 (4), 1421-1431 (2022)
   PUBMED   35114390
  REMARK    GeneRIF: NMNAT2 is downregulated in glaucomatous RGCs, and
            RGC-specific gene therapy rescues neurodegeneration and visual
            function.
REFERENCE   6  (bases 1 to 4650)
  AUTHORS   Ljungberg,M.C., Ali,Y.O., Zhu,J., Wu,C.S., Oka,K., Zhai,R.G. and
            Lu,H.C.
  TITLE     CREB-activity and nmnat2 transcription are down-regulated prior to
            neurodegeneration, while NMNAT2 over-expression is neuroprotective,
            in a mouse model of human tauopathy
  JOURNAL   Hum Mol Genet 21 (2), 251-267 (2012)
   PUBMED   22027994
  REMARK    GeneRIF: Decreased endogenous NMNAT2 function caused by reduced
            CREB signaling during pathological insults may be one of underlying
            mechanisms for neuronal death in tauopathies
REFERENCE   7  (bases 1 to 4650)
  AUTHORS   Mayer,P.R., Huang,N., Dewey,C.M., Dries,D.R., Zhang,H. and Yu,G.
  TITLE     Expression, localization, and biochemical characterization of
            nicotinamide mononucleotide adenylyltransferase 2
  JOURNAL   J Biol Chem 285 (51), 40387-40396 (2010)
   PUBMED   20943658
  REMARK    GeneRIF: Nmnat2 is a neuronal protein peripherally attached to
            membranes via palmitoylation and suggest that Nmnat2 is transported
            to synaptic terminals via an endosomal pathway.
REFERENCE   8  (bases 1 to 4650)
  AUTHORS   Yan,T., Feng,Y., Zheng,J., Ge,X., Zhang,Y., Wu,D., Zhao,J. and
            Zhai,Q.
  TITLE     Nmnat2 delays axon degeneration in superior cervical ganglia
            dependent on its NAD synthesis activity
  JOURNAL   Neurochem Int 56 (1), 101-106 (2010)
   PUBMED   19778564
  REMARK    GeneRIF: The brain-specific Nmnat2 delays injury-induced axon
            degeneration dependent on its NAD synthesis activity. These
            findings provide new clues to further study the molecular
            mechanisms of axon degeneration and the related neurodegenerative
            diseases.
REFERENCE   9  (bases 1 to 4650)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   10 (bases 1 to 4650)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC110038.25 and AC099735.47.
            
            On Mar 15, 2023 this sequence version replaced NM_175460.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089007.1, SRR7652917.313647.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-215               AC110038.25        120404-120618
            216-304             AC099735.47        108872-108960
            305-372             AC099735.47        109748-109815
            373-451             AC099735.47        111984-112062
            452-578             AC099735.47        121412-121538
            579-659             AC099735.47        123859-123939
            660-704             AC099735.47        125572-125616
            705-781             AC099735.47        129112-129188
            782-883             AC099735.47        147217-147318
            884-951             AC099735.47        147455-147522
            952-4650            AC099735.47        150643-154341
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 65.15 cM"
     gene            1..4650
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /note="nicotinamide nucleotide adenylyltransferase 2"
                     /db_xref="GeneID:226518"
                     /db_xref="MGI:MGI:2444155"
     exon            1..215
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..1054
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /EC_number="2.7.7.1"
                     /EC_number="2.7.7.18"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pyridine nucleotide adenylyltransferase 2; nicotinamide
                     mononucleotide adenylyltransferase 2; NMN
                     adenylyltransferase 2; naMN adenylyltransferase 1;
                     nicotinate-nucleotide adenylyltransferase 1; naMN
                     adenylyltransferase 2; NMN/NaMN adenylyltransferase 2;
                     nicotinate-nucleotide adenylyltransferase 2;
                     nicotinamide/nicotinic acid mononucleotide
                     adenylyltransferase 2; blad; protein bloated bladder"
                     /codon_start=1
                     /product="nicotinamide/nicotinic acid mononucleotide
                     adenylyltransferase 2 isoform 1"
                     /protein_id="NP_780669.1"
                     /db_xref="CCDS:CCDS15368.1"
                     /db_xref="GeneID:226518"
                     /db_xref="MGI:MGI:2444155"
                     /translation="MTETTKTHVILLACGSFNPITKGHIQMFERARDYLHKTGRFIVI
                     GGIVSPVHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWECYQDTWQTTCSVLEHH
                     RDLMKRVTGCILSNVNTPSMTPVIGQPQHENTQPIYQNSNVPTKPTAAKILGKVGESL
                     SRICCVRPPVERFTFVDENANLGTVMRYEEIELRILLLCGSDLLESFCIPGLWNEADM
                     EVIVGDFGIVVVPRDAADTDRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSTKSRLA
                     LQHGDGHVVDYLSQPVIDYILKSQLYINASG"
     exon            216..304
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            305..372
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            373..451
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            452..578
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            579..659
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            660..704
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            705..781
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            782..883
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            884..951
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            952..4650
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4627..4632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /note="hexamer: AATAAA"
     polyA_site      4650
                     /gene="Nmnat2"
                     /gene_synonym="D030041I09Rik; PNAT1; PNAT2"
                     /note="major polyA site"
ORIGIN      
        1 gcgtctagag cggctccttg gagtccacag catccaccgc cggagcctcg ccttcctttc
       61 tccgtctgca gacatatcga gacacaaaaa gaggcaaccc ctggatcagt gcgagagacc
      121 tcatcccatc atgaccgaga ccacaaagac ccacgttatc ctgctggcct gcggcagctt
      181 caatcccatc actaaagggc acattcagat gttcgagaga gccagggatt atctgcacaa
      241 gactggaaga tttattgtga ttggcgggat tgtctctccg gtccatgact cctacggaaa
      301 acagggcctt gtgtcaagtc ggcaccgtct catcatgtgt cagctggctg tccagaattc
      361 cgactggatc agggtggacc catgggagtg ctatcaggac acctggcaga caacctgcag
      421 tgtgttggag caccatcgag acctgatgaa gagggtgacc ggctgcatcc tctccaacgt
      481 caacacacct tccatgacac ctgtgatcgg acagccacag catgagaaca cccagcccat
      541 ttaccagaac agcaatgtgc ccaccaagcc cactgcagcc aagatcttgg gaaaggtggg
      601 agaaagcctc agccggatct gctgcgtccg cccaccagtg gagcgcttca cttttgtaga
      661 tgagaacgcc aacctgggca cagtgatgcg gtatgaggag atcgagctgc gcatcttgct
      721 gctgtgtggt agtgacctgc tggagtcttt ctgcatccca ggactctgga atgaggcaga
      781 tatggaagtg attgttgggg actttgggat cgtcgtggta cccagggatg cagcggacac
      841 agaccggatc atgaatcact cctccatact ccgcaagtac aaaaacaaca tcatggttgt
      901 gaaggatgat atcaaccatc ccatgtctgt agtcagctcc accaagagca ggctggccct
      961 gcagcatggg gacggccatg ttgtggatta cctgtcccag ccagtcatcg attacatcct
     1021 caagagtcag ctgtacatca acgcctcggg ctagcggata cccagctctc tgctctattc
     1081 tccccgtgtc ctctgccaac acactgtcag accccggttt ctctcctgcc tctctgtttc
     1141 tctgtcttca tcttgactgt tctccccact ggctgaccta acccccacag tgtggagggc
     1201 ctgcgaagaa ccgtggcgca ccccattcca cagtcatctt tggacaaact ttcctctagt
     1261 ctccgggttg ggggtgggta agggaacagg gtgggagttg gggaaagtgc agcccttgga
     1321 gatgtactgg tgtccgtatc ccagcatgct ctggggacat ggctcaggcc cttgccttcc
     1381 cagcatgccc tttaccacaa agggctattt ttttttcttt ttctttgtcc acttcttgta
     1441 gaacttggat gaaatcagtg tgtgtgtgag tgtgtttgtt tgtttgtagt cttgatgctc
     1501 tcctgtggtt tacttccctt ctgataggac actatagcca gtctcagcac tcgtaagtgc
     1561 ggagggccac atccaggaaa gaaggcagag cctctctgct tttccaatac acacacacac
     1621 acacacacac acacacacac acacacacac acacagcaca ccagcagcag catccccaga
     1681 cccctttggt caagagtaag actttcaggt tctgaaacct ggtacataag tctgggaaga
     1741 gtagggtttc tcagaatttg agtccctggt ttcagatgat cgtggtcttg atgctgtgtg
     1801 tctgaccagt agcattgagt gggttctctg ccaaccaggc atttgcagag agcaaaaacc
     1861 ctcggaggtc atgctaaggc atgttcctgt tggtgagagg aaccacacaa ggcaagacta
     1921 agtcctgata atctattctt tttttttttt ttttgaaaac atgctaagaa cccacttgac
     1981 tgaaaagatg ctttgctaat tggcattatt tattgcccat ttggctcgga gctataagtc
     2041 caggatgatc tgggagaatg cagtttgtta ccctgagaat tcatgatccc cacacactcc
     2101 acctgagctt gccaggctgt gcccaaagcc ctttccttct tgcaaagcaa tcagccttgt
     2161 ttctgagctg gccctacctg tctcctgcag agcccagcag tcaatgccct gggaggtctc
     2221 cctgacacac aagagcagtc tctcctgttt ctccccattt ctcctcgatg tcctcccctt
     2281 ctctctacct ctgaagacag ttcaagactg gacaagatac tcgtgtcctg cctggagagc
     2341 tcgtggggaa tgatttttgt gttttgtgtt tgcctttcag cctctggctc ttgggtttct
     2401 ggttgttcat ggggaacagc tcctgagtgt caggctacat tttaatttct aacatcaaca
     2461 tggcccaggt gggactggat caggggcgat gctttttgcc ttggctttcc ctcccacctt
     2521 ctccgctgac ttcaggagga agaagctggg tcaacagaag cgcataggcc atctccagag
     2581 ctgtggcttt tctttagact gtagaagtga cctccagagg gaggagatta tttctataat
     2641 cgagaagtcc agtgtttgac taaaagtcat ggaaaaatag agaaaacttg catgacccat
     2701 ctgctcccgg gttcttattt gaaaacaagg gagcagagac ccagggagtc agtgtcttgt
     2761 ccaggatcac tcagtgcatc ctgggaagta tgatgagaag atgctgcttc cctagacaaa
     2821 agaaaaccag gagcctgcgg ggagacatgg gtgacatggg cgacatgggg ttttgaaggc
     2881 tctagagctg acatggctcc acaagagcag accccagcaa ggctacttcc attttgttcc
     2941 tttggttcac tgtgtttctg ttctgaactc ttgttgagct tcattcagtc catagcctgg
     3001 gataaggaac caggttagcc cagcagcctc agggggcaac aggcagctca gaacagggaa
     3061 ggaaggagag gtgagctaag aaaagaatca cacagaagaa gtgcatttct gggaagcagg
     3121 tgggcagatt gtatcttgtt aaggcgtgtt aaaaatggtt tccccttaac agaggtgagt
     3181 ccccatccgg gtgggattgt ccattcactt ctctgtctct ttctcccgtt gttggatcaa
     3241 gggtagggga cccccccccg taccccccaa ccctgcccct aaggggagca caatgtgact
     3301 gtgacctgcc gtggccaaca ccaaaaagac agtttataag tggtgcctca ggtgtcatca
     3361 gggtgacttg ggtctgtctt tggggaccca ttgtgagagc tagcctatta ctggcatggc
     3421 acagagatgg taataaagac acaaactgca gtgatcccat catttactgc tgcctgccat
     3481 ttttaatgtc cccgccattt tttttaaagc agctgataga acagagagga caaactttaa
     3541 taaccctctc cttaaggaat gaggctgggc ctccctggat taattgtctg agccacacac
     3601 tccccatccc aggtagcctc tgggcccctg gctttcactt tccttgaatg tacattggac
     3661 cagcaaggga agtctcttac tgaagtgcct gaattcacag ctgaagtctt agagacactg
     3721 gccttcctgt tttggcttgg ctacaagttc tagtctccag tttcaggatc tctaaaagga
     3781 ttcatgaaca ctctattcaa ataagtggga agtaaaactt gataatcaaa ctgtatcaaa
     3841 agccagagga ttttagggag cccccaccct caccctcatc cctacccccg atgttctcat
     3901 acatgcttgt aggggactga gagaagcatg tccatgtatt tgtgttccta tttttaaaag
     3961 taacgcaaca ccaggtgtgg tggcacatac ctgtgatccc agcactgaga ggtggaggca
     4021 ggaggaccag gggttcaagc ccagcctctg ggcacagaag accttgtctc aaaacaaaag
     4081 cagcaatgta aaaccaggat gggacagtct caggacctct cctcgtgtta cagcaggagg
     4141 gctacaaaga gtggaggtgg gaagacttta tagcgcatgt aggtaggaca ccttggagat
     4201 atcctatggt aatgaggatg ggtgtactct gccacgctga tccaacctgt actgtcctgt
     4261 gagctgactg gtgaattcca ctcttgatgt ggtttctgtg tagtgtgcat ttggaagtgt
     4321 cctagtagcc tgttctagtt agttagactg cagccctttc ccatcacctt cagtggagtc
     4381 ccagggcaca cagctcatct cgatccttgt gggttggaat ggggaggagg aagctttttt
     4441 ttttttaatt ttttaaaaaa tcatatctgc taatttaaga gtgagatttg ctttatggaa
     4501 gtcaactcaa taaaaactgc attcgagttg caaataccat ttcacagtga aatatttttg
     4561 agtagagttc tgttgctagg atagtcgtgg gcaagagttt tatcagcaaa atgtttcaat
     4621 tatgttaata aatcagacaa tgctactaga
//
