LOCUS       NM_001167909            1704 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus NME/NM23 family member 8 (Nme8), transcript variant 2,
            mRNA.
ACCESSION   NM_001167909
VERSION     NM_001167909.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Smith,T.B., Baker,M.A., Connaughton,H.S., Habenicht,U. and
            Aitken,R.J.
  TITLE     Functional deletion of Txndc2 and Txndc3 increases the
            susceptibility of spermatozoa to age-related oxidative stress
  JOURNAL   Free Radic Biol Med 65, 872-881 (2013)
   PUBMED   23707457
  REMARK    GeneRIF: Txndc2 and Txndc3 are critically important in protecting
            spermatozoa against the increases in oxidative stress associated
            with paternal age.
REFERENCE   3  (bases 1 to 1704)
  AUTHORS   Duriez,B., Duquesnoy,P., Escudier,E., Bridoux,A.M., Escalier,D.,
            Rayet,I., Marcos,E., Vojtek,A.M., Bercher,J.F. and Amselem,S.
  TITLE     A common variant in combination with a nonsense mutation in a
            member of the thioredoxin family causes primary ciliary dyskinesia
  JOURNAL   Proc Natl Acad Sci U S A 104 (9), 3336-3341 (2007)
   PUBMED   17360648
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6490]
REFERENCE   4  (bases 1 to 1704)
  AUTHORS   Jimenez,A., Prieto-Alamo,M.J., Fuentes-Almagro,C.A., Jurado,J.,
            Gustafsson,J.A., Pueyo,C. and Miranda-Vizuete,A.
  TITLE     Absolute mRNA levels and transcriptional regulation of the mouse
            testis-specific thioredoxins
  JOURNAL   Biochem Biophys Res Commun 330 (1), 65-74 (2005)
   PUBMED   15781233
REFERENCE   5  (bases 1 to 1704)
  AUTHORS   Miranda-Vizuete,A., Tsang,K., Yu,Y., Jimenez,A., Pelto-Huikko,M.,
            Flickinger,C.J., Sutovsky,P. and Oko,R.
  TITLE     Cloning and developmental analysis of murid spermatid-specific
            thioredoxin-2 (SPTRX-2), a novel sperm fibrous sheath protein and
            autoantigen
  JOURNAL   J Biol Chem 278 (45), 44874-44885 (2003)
   PUBMED   12909633
  REMARK    GeneRIF: data indicate that spermatid-specific thioredoxin-2
            incorporation into the sperm fibrous sheath(FS) lags well behind FS
            assembly, suggesting it is required during the final stages of
            sperm tail maturation
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153016.2 and AC164102.2.
            
            Transcript Variant: This variant (2) lacks two alternate in-frame
            exons in the 3' coding region, compared to variant 1. The resulting
            isoform (2) lacks an internal segment near the C-terminus, compared
            to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052356.1, SRR5189685.212505.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849381,
                                           SAMN00849384 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               AC153016.2         157566-157704       c
            140-182             AC153016.2         156948-156990       c
            183-240             AC153016.2         156813-156870       c
            241-347             AC153016.2         156531-156637       c
            348-419             AC153016.2         154262-154333       c
            420-536             AC153016.2         150793-150909       c
            537-603             AC153016.2         150119-150185       c
            604-677             AC153016.2         148889-148962       c
            678-770             AC164102.2         233172-233264       c
            771-958             AC164102.2         230975-231162       c
            959-1134            AC164102.2         229734-229909       c
            1135-1279           AC164102.2         215840-215984       c
            1280-1387           AC164102.2         214101-214208       c
            1388-1539           AC164102.2         213247-213398       c
            1540-1704           AC164102.2         200413-200577       c
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 7.0 cM"
     gene            1..1704
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /note="NME/NM23 family member 8"
                     /db_xref="GeneID:73412"
                     /db_xref="MGI:MGI:1920662"
     exon            1..139
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            140..182
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..1562
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     thioredoxin domain-containing protein 3;
                     spermatid-specific thioredoxin-2; thioredoxin domain
                     containing 3 (spermatozoa); 3'-5' exonuclease NME8"
                     /codon_start=1
                     /product="thioredoxin domain-containing protein 3 isoform
                     2"
                     /protein_id="NP_001161381.1"
                     /db_xref="CCDS:CCDS49208.1"
                     /db_xref="GeneID:73412"
                     /db_xref="MGI:MGI:1920662"
                     /translation="MASKKREVQLQSVVNSQNLWDEMLLNKGLTVIDVYQAWCGPCKA
                     VQSLFRKLKNELNEDEILHFVVAEADNIVTLQPFRDKCEPVFLFSLNGKIIAKIQGAN
                     APLINRKVITLIDEERKIVAGEMDRPQYVEIPLVDAIDEEYGEVQYESAAEVYNMAII
                     KPDAVLMRKNIEVREKIAKEGFVIEIQENLILPEEVVREFYTHIADQPDFEEFVVSMT
                     NGLSCVLIVSQEDSEVIQEETLPQTDTEEEPGVLEEPHVRFAPVMIKKKRDSLQEYMD
                     RQHMSDYCDVEDDAVKVSKLIDILFPDFKTMKSTNVQTTLALLHPDICEEEKDDVLNV
                     IHNEGFTILMQRQIVLSEEEARTVCKIHENEEYFDNLIGHMTSNHSYVLALRRENGVE
                     YWKTLIGPKTIEEAYASHPQSLCVQFASGNFPTNQFYGSSSKAAAEKEIAHFFPPQST
                     LALIKPHVTHKERIHRSSRR"
     exon            183..240
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            241..347
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            348..419
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            420..536
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            537..603
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            604..677
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            678..770
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            771..958
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1134
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1279
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1387
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1539
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1704
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1683..1688
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /note="hexamer: AATAAA"
     polyA_site      1704
                     /gene="Nme8"
                     /gene_synonym="1700056P15Rik; Sptrx-2; Txndc3"
                     /note="major polyA site"
ORIGIN      
        1 aacgttggcg tccttgccct tcatcttcca gggggtgatg ctaggcatac ctcatccctc
       61 cctttgactg gacctgctgg ggaagaaggc acagcaggag ccctgccacc ttcagcccgg
      121 gcctgaccca gcggtccaga agtcgacaaa tggcaagcaa aaagcgtgaa gtccagctac
      181 agtcagtcgt caatagtcag aacttgtggg atgagatgtt gctgaacaaa ggcttaacag
      241 tgattgatgt ttaccaagcc tggtgtggac cttgcaaagc cgtgcaaagt ttattcagaa
      301 aactaaaaaa tgaactgaac gaagatgaga ttcttcactt cgtcgttgct gaagctgaca
      361 acattgtgac tctccagcca tttagagata aatgtgagcc cgtgtttctc tttagtctta
      421 atggtaaaat cattgcaaag attcagggtg caaatgctcc acttatcaat agaaaagtca
      481 ttaccttgat agatgaagag aggaaaattg tagcaggtga aatggatcgt cctcagtatg
      541 ttgaaattcc actagtagat gcaatcgatg aagaatatgg ggaagtacag tatgaaagtg
      601 ctgcggaagt ttacaatatg gcaattatca aacctgatgc tgtactcatg agaaaaaata
      661 tagaagttag ggaaaaaata gccaaagaag gatttgttat agaaatacaa gaaaacctga
      721 ttctccctga agaggtagtg agggaattct acactcatat agcagaccag cctgactttg
      781 aagagtttgt cgtttctatg acaaatggcc tcagctgtgt gctcattgta tctcaagaag
      841 actccgaggt tattcaggaa gaaactctcc cgcagactga tacagaagaa gaacctggcg
      901 ttttggaaga gcctcacgtt aggtttgcac ctgtgatgat aaagaagaaa cgggacagtt
      961 tgcaagagta tatggaccga cagcatatgt ctgattactg cgatgtcgag gacgatgcgg
     1021 ttaaggtctc taagctcatt gacatattat tccctgattt taaaactatg aaaagcacga
     1081 atgtacaaac gacgctagca ttactgcatc cagacatctg tgaggaagag aaagatgacg
     1141 tgttgaacgt tattcacaat gaagggttca ccatactgat gcagaggcaa atcgtattat
     1201 cagaggaaga agcaagaaca gtgtgcaaga tccatgaaaa cgaagagtat tttgataatc
     1261 ttatagggca catgaccagt aatcactctt atgtccttgc tctacggagg gaaaatggtg
     1321 tggaatattg gaaaacatta attgggccaa aaacgattga ggaagcttat gcatctcatc
     1381 cacagagttt atgtgtacag tttgcttcag ggaattttcc taccaaccag ttctacggga
     1441 gcagttcaaa agcagcagct gagaaggaaa tagcgcattt cttccctccc cagagcacac
     1501 ttgcattgat caagcctcat gtgacacaca aagaaagaat tcacagaagc tcaaggaggt
     1561 aatgaagaag ttttcatcaa cgtaaaacca cgagacacaa ctttctataa acagtaagaa
     1621 gaaaaatgac tccaaggaaa acacacatgg agatgaaaac ttgcctggct atgcatggta
     1681 acaataaaaa ctgtgacccc caaa
//
