LOCUS       NM_026523                692 bp    mRNA    linear   ROD 31-DEC-2024
DEFINITION  Mus musculus neuromedin B (Nmb), transcript variant 2, mRNA.
ACCESSION   NM_026523
VERSION     NM_026523.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Jiang,H., Cui,H., Chen,M., Li,F., Shen,X., Guo,C.J., Hoekel,G.E.,
            Zhu,Y., Han,L., Wu,K., Holtzman,M.J. and Liu,Q.
  TITLE     Divergent sensory pathways of sneezing and coughing
  JOURNAL   Cell 187 (21), 5981-5997 (2024)
   PUBMED   39243765
REFERENCE   2  (bases 1 to 692)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 692)
  AUTHORS   Guo,C., Jiang,H., Huang,C.C., Li,F., Olson,W., Yang,W., Fleming,M.,
            Yu,G., Hoekel,G., Luo,W. and Liu,Q.
  TITLE     Pain and itch coding mechanisms of polymodal sensory neurons
  JOURNAL   Cell Rep 42 (11), 113316 (2023)
   PUBMED   37889748
REFERENCE   4  (bases 1 to 692)
  AUTHORS   Souza,G.M.P.R., Stornetta,D.S., Shi,Y., Lim,E., Berry,F.E.,
            Bayliss,D.A. and Abbott,S.B.G.
  TITLE     Neuromedin B-Expressing Neurons in the Retrotrapezoid Nucleus
            Regulate Respiratory Homeostasis and Promote Stable Breathing in
            Adult Mice
  JOURNAL   J Neurosci 43 (30), 5501-5520 (2023)
   PUBMED   37290937
REFERENCE   5  (bases 1 to 692)
  AUTHORS   Cikes,D., Atanes,P., Cronin,S.J.F., Hagelkruys,A., Huang,G.C.,
            Persaud,S.J. and Penninger,J.M.
  TITLE     Neuropeptide Neuromedin B does not alter body weight and glucose
            homeostasis nor does it act as an insulin-releasing peptide
  JOURNAL   Sci Rep 12 (1), 9383 (2022)
   PUBMED   35672347
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 692)
  AUTHORS   Fleming,M.S., Ramos,D., Han,S.B., Zhao,J., Son,Y.J. and Luo,W.
  TITLE     The majority of dorsal spinal cord gastrin releasing peptide is
            synthesized locally whereas neuromedin B is highly expressed in
            pain- and itch-sensing somatosensory neurons
  JOURNAL   Mol Pain 8, 52 (2012)
   PUBMED   22776446
  REMARK    GeneRIF: The majority of dorsal spinal cord Grp is synthesized
            locally in dorsal spinal cord neurons while Nmb is highly expressed
            in pain- and itch-sensing dorsal root ganglion neurons.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 692)
  AUTHORS   Zhang,W.S., Fei,K.L., Wu,M.T., Wu,X.H. and Liang,Q.H.
  TITLE     Neuromedin B and its receptor influence the activity of myometrial
            primary cells in vitro through regulation of Il6 expression via the
            Rela/p65 pathway in mice
  JOURNAL   Biol Reprod 86 (5), 154,1-7 (2012)
   PUBMED   22262690
  REMARK    GeneRIF: The data demonstrated that the neuromedin B-neuromedian B
            receptor interaction in pregnant myometrial primary cells in vitro
            predominantly influenced uterine activity through regulation of Il6
            expression via the Rela/p65 pathway.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 692)
  AUTHORS   Cheng,T.W. and Gong,Q.
  TITLE     Secreted TARSH regulates olfactory mitral cell dendritic complexity
  JOURNAL   Eur J Neurosci 29 (6), 1083-1095 (2009)
   PUBMED   19302145
REFERENCE   9  (bases 1 to 692)
  AUTHORS   Hoggard,N., Bashir,S., Cruickshank,M., Miller,J.D. and
            Speakman,J.R.
  TITLE     Expression of neuromedin B in adipose tissue and its regulation by
            changes in energy balance
  JOURNAL   J Mol Endocrinol 39 (3), 199-210 (2007)
   PUBMED   17766645
  REMARK    GeneRIF: REsults describe the expression of neuromedin B in adipose
            tissue and its regulation by changes in energy balance.
REFERENCE   10 (bases 1 to 692)
  AUTHORS   Kopan,S., Sivasubramaniam,U. and Warburton,M.J.
  TITLE     The lysosomal degradation of neuromedin B is dependent on
            tripeptidyl peptidase-I: evidence for the impairment of
            neuropeptide degradation in late-infantile neuronal ceroid
            lipofuscinosis
  JOURNAL   Biochem Biophys Res Commun 319 (1), 58-65 (2004)
   PUBMED   15158442
  REMARK    GeneRIF: lysosomal degradation of neuromedin B is dependent on
            tripeptidyl peptidase-I
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB058776.1 and AC123744.8.
            
            On Apr 1, 2014 this sequence version replaced NM_026523.3.
            
            Summary: This gene encodes a member of the neuromedin family of
            neuropeptides. The encoded protein is a precursor that is
            proteolytically processed to generate a biologically active
            neuropeptide that plays a role in satiety, reproduction and
            thermoregulation, as well as in stress, fear and other behavioral
            responses. This gene encodes distinct isoforms, some or all of
            which may undergo similar processing to generate the mature
            protein. [provided by RefSeq, Sep 2016].
            
            Transcript Variant: This variant (2) uses an alternate splice
            junction at the 5' end of the last exon compared to variant 1, that
            causes a frameshift. The resulting isoform (2) has a shorter and
            distinct C-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB058776.1, AK011929.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164136, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-647               CB058776.1         13-659
            648-692             AC123744.8         42655-42699         c
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 45.71 cM"
     gene            1..692
                     /gene="Nmb"
                     /gene_synonym="3110023K12Rik"
                     /note="neuromedin B"
                     /db_xref="GeneID:68039"
                     /db_xref="MGI:MGI:1915289"
     exon            1..242
                     /gene="Nmb"
                     /gene_synonym="3110023K12Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..451
                     /gene="Nmb"
                     /gene_synonym="3110023K12Rik"
                     /note="isoform 2 preproprotein is encoded by transcript
                     variant 2"
                     /codon_start=1
                     /product="neuromedin-B isoform 2 preproprotein"
                     /protein_id="NP_080799.1"
                     /db_xref="CCDS:CCDS21400.1"
                     /db_xref="GeneID:68039"
                     /db_xref="MGI:MGI:1915289"
                     /translation="MTRQAGSSWLLRGLLLFALFASGVAPFNWDLPEPRSRASKIRVH
                     PRGNLWATGHFMGKKSLEPPSLSLVGTAPPNTPRDQRLQLSHDLLRILLRKKALGMNF
                     SGPAPPIQYRRLLEPLLQK"
     exon            243..415
                     /gene="Nmb"
                     /gene_synonym="3110023K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            416..692
                     /gene="Nmb"
                     /gene_synonym="3110023K12Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      666..671
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nmb"
                     /gene_synonym="3110023K12Rik"
                     /note="hexamer: AATAAA"
     polyA_site      685
                     /gene="Nmb"
                     /gene_synonym="3110023K12Rik"
                     /note="major polyA site"
ORIGIN      
        1 cccgactgta gtttaaaagg ctggaaggcg ggcttcgaga actagtatcc tgagagtgcc
       61 agagaagagc ctgtttggca cagccatgac ccggcaagca gggagctctt ggctcctccg
      121 tggtctcctg ctcttcgcat tgttcgcttc cggcgtcgct cccttcaact gggatctccc
      181 ggagccccgc agccgagcaa gcaagattcg agtgcaccct cggggcaacc tctgggcgac
      241 cggtcacttc atgggcaaga agagcctgga acccccgagc ctgtcactgg tggggacagc
      301 accccctaac accccgaggg accagagact acagctgagt catgatctgc tcaggatcct
      361 cctgcgaaag aaagctctag gcatgaactt cagtggccca gctcccccaa tccagtacag
      421 gaggctgctg gagccactac tgcagaagtg atgccaataa tggaacaaac cggatgctgg
      481 gcttagaatg tgtccatcca gggaagctga caatggaacc ctagcagtgg cctcctctgg
      541 atgtaaatcc taagctcaaa tgtgttaatc tgttactgtg atttctggtt gggtcaccag
      601 gaatgttaat gatgcagaca caaagtcttt cctgctgtat ttcttgcttc cctggtgaag
      661 tggtgaataa aaatactgct ctttacacag aa
//
