LOCUS       NM_001357096           15342 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus neuroligin 1 (Nlgn1), transcript variant 4, mRNA.
ACCESSION   NM_001357096
VERSION     NM_001357096.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 15342)
  AUTHORS   Benner,O., Cast,T.P., Minamide,L.S., Lenninger,Z., Bamburg,J.R. and
            Chanda,S.
  TITLE     Multiple N-linked glycosylation sites critically modulate the
            synaptic abundance of neuroligin isoforms
  JOURNAL   J Biol Chem 299 (12), 105361 (2023)
   PUBMED   37865312
REFERENCE   2  (bases 1 to 15342)
  AUTHORS   Bemben,M.A., Shipman,S.L., Nicoll,R.A. and Roche,K.W.
  TITLE     The cellular and molecular landscape of neuroligins
  JOURNAL   Trends Neurosci 38 (8), 496-505 (2015)
   PUBMED   26209464
  REMARK    Review article
REFERENCE   3  (bases 1 to 15342)
  AUTHORS   Hu,X., Luo,J.H. and Xu,J.
  TITLE     The interplay between synaptic activity and neuroligin function in
            the CNS
  JOURNAL   Biomed Res Int 2015, 498957 (2015)
   PUBMED   25839034
  REMARK    Review article
REFERENCE   4  (bases 1 to 15342)
  AUTHORS   Mackowiak,M., Mordalska,P. and Wedzony,K.
  TITLE     Neuroligins, synapse balance and neuropsychiatric disorders
  JOURNAL   Pharmacol Rep 66 (5), 830-835 (2014)
   PUBMED   25149987
  REMARK    Review article
REFERENCE   5  (bases 1 to 15342)
  AUTHORS   Bang,M.L. and Owczarek,S.
  TITLE     A matter of balance: role of neurexin and neuroligin at the synapse
  JOURNAL   Neurochem Res 38 (6), 1174-1189 (2013)
   PUBMED   23559421
  REMARK    Review article
REFERENCE   6  (bases 1 to 15342)
  AUTHORS   Reissner,C., Runkel,F. and Missler,M.
  TITLE     Neurexins
  JOURNAL   Genome Biol 14 (9), 213 (2013)
   PUBMED   24083347
  REMARK    Review article
REFERENCE   7  (bases 1 to 15342)
  AUTHORS   Xu,J.Y., Xia,Q.Q. and Xia,J.
  TITLE     A review on the current neuroligin mouse models
  JOURNAL   Sheng Li Xue Bao 64 (5), 550-562 (2012)
   PUBMED   23090496
  REMARK    Review article
REFERENCE   8  (bases 1 to 15342)
  AUTHORS   Lise,M.F. and El-Husseini,A.
  TITLE     The neuroligin and neurexin families: from structure to function at
            the synapse
  JOURNAL   Cell Mol Life Sci 63 (16), 1833-1849 (2006)
   PUBMED   16794786
  REMARK    Review article
REFERENCE   9  (bases 1 to 15342)
  AUTHORS   Gilbert,M., Smith,J., Roskams,A.J. and Auld,V.J.
  TITLE     Neuroligin 3 is a vertebrate gliotactin expressed in the olfactory
            ensheathing glia, a growth-promoting class of macroglia
  JOURNAL   Glia 34 (3), 151-164 (2001)
   PUBMED   11329178
REFERENCE   10 (bases 1 to 15342)
  AUTHORS   Scheiffele,P., Fan,J., Choih,J., Fetter,R. and Serafini,T.
  TITLE     Neuroligin expressed in nonneuronal cells triggers presynaptic
            development in contacting axons
  JOURNAL   Cell 101 (6), 657-669 (2000)
   PUBMED   10892652
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC100381.9, AC099863.14 and AC127365.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660825.175611.1,
                                           SRR1660821.193094.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-722               AC100381.9         145840-146561       c
            723-1533            AC100381.9         125817-126627       c
            1534-1686           AC099863.14        89193-89345
            1687-1899           AC127365.4         156923-157135       c
            1900-2689           AC127365.4         152911-153700       c
            2690-15342          AC127365.4         138866-151518       c
FEATURES             Location/Qualifiers
     source          1..15342
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 10.38 cM"
     gene            1..15342
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /note="neuroligin 1"
                     /db_xref="GeneID:192167"
                     /db_xref="MGI:MGI:2179435"
     exon            1..722
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /inference="alignment:Splign:2.1.0"
     exon            723..1533
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /inference="alignment:Splign:2.1.0"
     CDS             1041..3512
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /note="isoform 4 is encoded by transcript variant 4"
                     /codon_start=1
                     /product="neuroligin-1 isoform 4"
                     /protein_id="NP_001344025.1"
                     /db_xref="GeneID:192167"
                     /db_xref="MGI:MGI:2179435"
                     /translation="MALPRCMWPNYVWRAMMACVVHRGSGAPLTLCLLGCLLQTFHVL
                     SQKLDDVDPLVTTNFGKIRGIKKELNNEILGPVIQFLGVPYAAPPTGEHRFQPPEPPS
                     PWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYV
                     PTEDDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLASYGNVIVITVNYRLGVLGFL
                     STGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGAGGSCVNLLTLSHYS
                     EGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARILATKVGCNVSDTVELVECL
                     QKKPYKELVDQDVQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEG
                     LKFVENIVDSDDGVSASDFDFAVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRHNPE
                     TRRKTLLALFTDHQWVAPAVATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDE
                     VPYVLGIPMIGPTELFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTK
                     PNRFEEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELVPHLHNLNDISQYTS
                     TTTKVPSTDITLRPTRKNSTPVTSAFPTAKQDDPKQQPSPFSVDQRDYSTELSVTIAV
                     GASLLFLNILAFAALYYKKDKRRHDVHRRCSPQRTTTNDLTHAPEEEIMSLQMKHTDL
                     DHECESIHPHEVVLRTACPPDYTLAMRRSPDDIPLMTPNTITMIPNTIPGIQPLHTFN
                     TFTGGQNNTLPHPHPHPHSHSTTRV"
     exon            1534..1686
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1899
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2689
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /inference="alignment:Splign:2.1.0"
     exon            2690..15342
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5291..5296
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /note="hexamer: ATTAAA"
     polyA_site      5309
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /note="major polyA site"
     regulatory      6050..6055
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /note="hexamer: AAGAAA"
     polyA_site      6071
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
     regulatory      10534..10539
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /note="hexamer: ATTAAA"
     polyA_site      10561
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
     regulatory      15316..15321
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /note="hexamer: AATAAA"
     regulatory      15320..15325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
                     /note="hexamer: AATAAA"
     polyA_site      15336
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
     polyA_site      15342
                     /gene="Nlgn1"
                     /gene_synonym="6330415N05Rik; mKIAA1070; NL1; Nlg1"
ORIGIN      
        1 ctgcaccgat ttggagtaga aacctgtatt aggggaaaac cagggtcctc ggcatttttt
       61 tcttttcttt ctttcttttt tttcttttgc gagcagtgca cccagaatcc aaaaagattt
      121 ttaaaaaatt aaaaaaggca ggaaggaaag aagaaaagga aaggaaagaa agaaaccaca
      181 gcccttcggt aacccggata gggctacgga atcgttctac tggacgcgag cactggggat
      241 tcatctttga ggtcaatttc caatcgagat aaatctattg gattagtagg attatctgat
      301 cgcagctcaa agaagtaacg ttgtttgatc gtttgtattt cttgcctgat ggtaggaatt
      361 acacggatgc ggctttggtt ctccaggatt ctggatcctg gcagcatctg gagagaaaaa
      421 aagcatcaat ccacacgttt aggaagtgtc cgtgggtgaa taacgaggga gggggaggtg
      481 gatctggggt tgcggctgct taaagaaaaa aaaaataaat aaataaagga agcatcttga
      541 attctgaaac aagaattacc cagttccaga ggttgaaaag aaagcatctg caaactggta
      601 aatactttga ccgtttcggc ttgaatttga cgggaagtgt tggtaaaact gtctccttga
      661 ataatggaaa gaggactggg ggaagatgag ggggctccct cggtggacgt gtcttcctga
      721 gggatataga cccactgcta cctggatttg atttatacaa gagaaatcta catcaatggc
      781 cactcttacc acattgctac tatgggaaac agaatggtca ataggagatg tcctgatgaa
      841 tagtgatgat tgaagatgcg gcatctgtct gtgtgaaatc aatgggagct acttactgca
      901 tgaagagtgc tttgagcatt ccttcacaag ctcatatttg agaaactgga gctgcattct
      961 accaccattc catctttctc cagtggatac aaatatactt gccttgctgt accagacaaa
     1021 tagacaacta atgtgggacc atggcacttc ccagatgcat gtggccaaat tatgtttgga
     1081 gagctatgat ggcatgtgtg gtccacaggg gatccggtgc cccattgact ctctgcttgt
     1141 tgggatgttt gctacagact tttcacgtac tctctcaaaa gttggatgat gtagacccat
     1201 tggttactac taactttggc aagattaggg gaattaagaa agaactcaat aatgaaattt
     1261 tgggtcctgt cattcagttt cttggggttc catatgccgc tccaccaaca ggagaacatc
     1321 gtttccagcc tccagaacca ccatctccct ggtctgacat ccggaacgcc actcagtttg
     1381 ctcctgtatg tccccagaat atcattgatg gcagattgcc tgaagttatg cttcctgtgt
     1441 ggttcactaa taacttggat gtggtttcat catacgtcca agaccagagt gaagactgtc
     1501 tatacttaaa catctatgtc ccaactgaag atgatattcg ggacagtggg ggtcccaaac
     1561 cagtgatggt gtacatccat ggcggctctt acatggaagg tactggaaat ctgtatgatg
     1621 ggagtgtctt ggcaagctat ggcaatgtga tcgtcatcac agtcaactat cggcttgggg
     1681 tacttggctt cttgagcaca ggggatcagg ctgccaaagg aaactacggg ctccttgacc
     1741 tcatccaggc cctaagatgg accagcgaga acattgggtt ctttggtggt gaccccttgc
     1801 gaatcactgt gtttggatca ggcgctgggg gttcatgtgt caacctgctg actttatccc
     1861 attattctga aggtaaccgt tggagcaatt caaccaaagg actttttcaa cgagcaatag
     1921 ctcagagtgg aacagccctt tccagctggg ctgttagttt ccagcctgca aaatacgcta
     1981 gaattctggc cacaaaagtt ggctgcaatg tttcagatac agtagagtta gtagaatgcc
     2041 tgcagaagaa gccttacaaa gaacttgttg atcaagatgt tcaaccagcc cgataccaca
     2101 tagcctttgg acctgtgatc gatggtgatg taataccaga tgaccctcag atactgatgg
     2161 aacaaggaga gttcctcaac tatgatataa tgttgggagt taaccaaggg gaagggttga
     2221 agtttgtcga aaatatagta gatagtgatg atggtgtatc agccagtgat ttcgactttg
     2281 ctgtttctaa ttttgttgat aatttatatg gatatcctga aggcaaagat gttttgagag
     2341 aaaccattaa attcatgtat actgactggg ctgatcgcca taatcctgaa actagaagga
     2401 agacattgtt ggctttgttt acggaccatc aatgggtagc acctgctgtg gccacagcag
     2461 accttcactc gaactttggc tcacctacat acttctatgc cttttatcat cattgccaaa
     2521 cagatcaagt tccagcttgg gctgatgcag ctcatgggga tgaggttccc tatgtgttgg
     2581 gaatccccat gattggccct acagagttat ttccttgcaa tttctccaag aatgatgtga
     2641 tgttgagtgc agtagtaatg acatactgga cgaattttgc taaaactggt gacccaaatc
     2701 aaccagttcc tcaagacaca aaattcatcc ataccaaacc caaccgcttt gaagaagtag
     2761 catggaccag atattcccag aaagaccagc tttatctcca tattggatta aaaccgagag
     2821 ttaaagagca ttacagagcc aataaggtaa atctctggct ggagctggta cctcatctgc
     2881 ataatctcaa tgacatttct cagtatacct cgacaacaac taaagtgcca tccacggaca
     2941 tcactctcag acctacaagg aagaattcca caccagtcac atcagccttt cccactgcca
     3001 aacaggatga tcccaagcaa caaccaagcc ccttctcggt ggatcagagg gactactcca
     3061 cagagctaag tgtcactatc gcagtggggg cctctctgct gtttctcaac atcttggctt
     3121 ttgcagccct gtactacaag aaggataaga ggagacatga tgtccaccgg aggtgcagcc
     3181 ctcagcgcac gaccaccaac gacctaaccc atgctccaga agaggaaatt atgtctctcc
     3241 aaatgaagca cactgacttg gatcacgagt gtgagtccat ccatccacat gaggtggttc
     3301 ttcggaccgc ctgtccccca gattatactc tagctatgag gaggtcacct gatgatattc
     3361 cactaatgac acctaacacc atcacaatga ttcccaacac tataccaggg attcagccct
     3421 tacatacatt caacacattt actggaggac agaataatac actgccccat ccccacccac
     3481 acccccattc acattcaaca accagggtat agccagatga gagagacaaa ctgatttttt
     3541 tttctgatgg agtgcagtaa aagattattg gaagattcct tggctttcaa ctcggaagac
     3601 tctcactatt taaataagga ggaatattat gtgaatatac atatcaagaa ctttgggggt
     3661 tttgaaaaat gaattgtata tagatataca cacaaatcaa ctttaaaaaa aaaagcaaat
     3721 tccaattgcg tgaagcaatt gttctgaatg atactttttc attcactttc aagaattaat
     3781 ttgttgaaga tttaagttac ttaacggagt taggcatgtg gaacaccaaa caggaaagta
     3841 ctgtgtctga atactttaaa aacaaacaaa aaacaaaaac aaaaacaaaa ataaaatcaa
     3901 ctatgaatat gcacaaggga cacaccaatg gaatgtcaga taatttcgcc agttttcatt
     3961 tggagccctt taattgtgta gaccatattt gcttaagtac acaggacacc tatgctgtta
     4021 atggcatagc agaagttcat gctgaaaatt gccagtaaaa caaagaagtt gccataagac
     4081 tggcaggtac aacattatca cataagtgct gtcagtacta agtcgtgggg atcaaggaga
     4141 ctggatattt ttagcacgat gtgcatgata atttatatgc ttggtggctg tgctgctggt
     4201 taagccgtaa ttaaaattct tctcatccca ttggagtttt taataggagc ttcctccatc
     4261 aattggcaga aactaaagaa gatattataa ggggcaaaag taattactat aaaataattt
     4321 gcagtagttt tgataataga gggaatagtt attaaagtct tgggagaaac tagaggtata
     4381 gtggtgaata ctcactgata tgaatcccag aaaaaaaatt ctataagttt aatattcttt
     4441 tcattccctt atatgtaatt tatagaaata caactctaat tgaacatgtg ttagctgcag
     4501 agctataatt tgctgtgggt ttgttactct gaagtttgct cctttcgtta acagtcttgc
     4561 aaggtgtaag gatgccccaa cagtaccatt ctgattacag ttctcagtcg attgatttac
     4621 tcatgttgca tgacaaaatg tttactacta agaattcaat agaaagcata gtccctcttc
     4681 acattgcatc tttctcgctg agggtctctt gaatttactc atgagatctc agtagaggaa
     4741 caatgtaggt gcagaaccta ggagagtcaa cagctggagg gtttgtcttt cacatatgtg
     4801 caatttcaaa tgaattctcg aagaccacag gaatctctga atcccctgtt gtttaccagt
     4861 aatatcagta tatcacagac ctttccaaat gtttgtatat gtaatcagat gtacatttat
     4921 attaaaaaaa aaaacaatga gatggacctc aagagcacat gctgatcaat ggtttctctc
     4981 ttatctgtac tatatttcta ttagactaaa gttatgtgat ttttttttac cttttttcag
     5041 atgactagca attttgatag tttgtaagat aatgcaaagg actttctctg actaactgct
     5101 gtaacagaaa cctttctttc cagctaccct ttttcaagaa tcgaaagctt attatacaaa
     5161 agcaaacact tgtatactac tcggtgggaa ggatgctgtg tatcacgacc acatccgtgg
     5221 tccttgggtg aaataaagat aatatggaga atggcgatta gtcaaatgct aagagacatg
     5281 atctttgctc attaaagagc taaaacgttt attgctgttt tgtcttttat tttgttttta
     5341 caaaaagaaa agaaaagaaa gaaatctaaa acaaaagaaa caagattgtg ttggagtctt
     5401 tttttttttt tttttttttt tttttttaga acagttcgat tttggaagac atataggcaa
     5461 ctgcagaact ctgcatggag ggagactctg ggaactttag tactacctta gaacctggaa
     5521 ctcatagaaa atgttacctt ggtaatttta aatactcctt cccctagaca tcttacaaac
     5581 atggtgacca ttgttctcac taaaaaaaaa aaaaaaaaac acaagttctg atgttgctca
     5641 ccagggaaaa agtctattgg aaatgtggaa atgaagggac attacctcat gaagtaaggt
     5701 atctgcttat aatataggag tggttagcct atcatatact taaaaaaatt cttcaagaga
     5761 attggaaagt gctaaaaaag atattgagct agcattaaaa aaaatctact gtgagaaact
     5821 tatttgatta tagcttccaa agtactaaac atcaatacgt ttaagtactg ttccacttaa
     5881 aactacaatt ctttctcata agcaaagtct tcaaaatccc gagtagtcta ctcaccactg
     5941 atgagtgacg agctcatact tacgagaatg tgttgccact gacagtgaac aaaaagaact
     6001 atgtagcctc tgaaggactg tttaatgcca aactgtcttt tactacatta agaaaacaat
     6061 aaaactagaa ataaaggtga cagcaaaatg tactgtgagt gagtgctttc atacacagca
     6121 attactttac ttggaaaaat cattctatta ggttatagtg gttgctctgt ttctatctta
     6181 ctataaaaac ctgctacatc ttactaacac ctactttttg acagcacaat tcatgcttca
     6241 tattaattta gaaaaatatt taagaaaata attcataacc tgattgttta ctgaaaggaa
     6301 acaaacagtc agatttttag ggctgccctc ttggggtgtg gcaaaggtat tgtgagtcac
     6361 tgtgagtagc tagtctagtt tgcattacca gactcgcttc ctgaacataa aattgtgtct
     6421 tgtacaacca gaactaacaa ctgtttgcaa actgaaatat aatttttaaa tgaaagcaaa
     6481 aatttggcat tttgattcgg ttctatctaa tatgtttgga ttgtgtttat ctagaagata
     6541 atatcctgtt ggggaaaagc taatactaaa tttaataaat attgtagcat tatttattaa
     6601 ttcagattgt cttggttgaa attaaatagc aacaaacaag gatgagattc ttagaatcca
     6661 tgatgacatc aaaagccaag ataagttttt gctgctttga ttttatataa ttttgccaac
     6721 atttatcaaa gacttattga tgttttccta taaacttagg gatgttcaaa tggcagattt
     6781 aatggtttta aatgttattg atatgttgag tgtggatcaa atttagttta ctatagatat
     6841 ttaataaaat tgattactaa cttttatgaa atattgaata taattttttg aacatcacaa
     6901 gttcccaatt tggagagctc agagaggaaa ttatttcatt cattttcaat ctaagaacat
     6961 actttaatgc acacctttgg acacatatta aaagaataca atgtatacat tttttttaaa
     7021 ttcccatgta aaaatcccat gtaaaattac atatgatact aagtaggcaa aatgcagtgt
     7081 agagcagtca ttctgttaca gaagtggaat gtcagcactc agtttttata tccatactta
     7141 taacatcagt gattttgtct gttggtacag ttgtactgga tgcatatgaa catccagaaa
     7201 ggatacagag agttgggcag atggtgctgg ctttacaggc atcagagttt cgggatcaat
     7261 ggcatctttt ctttggcacg tctcctttaa gtattttcac aagaacaaat aatgaatgga
     7321 atccctagga agttcacaat aactatggca gttgtcttgt aattttgctt acttcttagt
     7381 gattggttca aacttctgtc cttttatttc aagtgattag ttgttataga ctatcacttt
     7441 ttaatgtgag tgaatatttg ataatttggt tatgcttgct ccaaaacaaa tctaattaat
     7501 gggcacttcc tgctgagatt tgttatcatt cacttaatta tcttgccaag ctaactgttg
     7561 gagcagttgt catcatcttt tagtcaatat agtaatgaaa tgtctccaat agccttctgc
     7621 ttctcctttg cttgacatgt caacaacaca aacttcagct tttcatcaaa attctaagct
     7681 ccctactcat gtgatagatt gggcttcctt gatttattcc tataaattat cctataaatt
     7741 atgaattggt cttattttag cactcagttg tatttttatt tagggcagaa atcaattcaa
     7801 tttgtttagc actacagaaa atacaaactg gaaaatccac ctgtggtgct taagtgcctt
     7861 ctccttttgt tattatgttt agaaatcttc atttgattta aaatttataa ctcctgataa
     7921 tgccacttaa aagataaaac agaatggata aggtgttaat tgccaataaa ggaaatctat
     7981 tgggaaggtc tgggtttaat ttgagtttgt attatataaa aaaaaaaaaa aaaaaaaaaa
     8041 aactcccaat attcctctga aaagaccgaa gtaatttttt ggaataaatg gtaattagtt
     8101 tggcatctga ttttaatggt gctaaagaat ataactttac tattctgaga gactttaatt
     8161 tttttcagaa ccaaaggtgg tggtatatac ctttcattcc agtacttcag atgcaaagga
     8221 agacatattt ttacgagttc aaggccaact tatgtctata tggccagcta gtgctacaga
     8281 gtataccttt tctccaaaaa aaatttcaac tgggtaaaat tattttagaa ggaggcaatt
     8341 tttataaatg aacacacaat tgacacatat ctaattttta tctagtcatc aattacatcc
     8401 tgtatggtat gatactctgt atacaagtta tagaattcat aggatggtcc tttcagatgt
     8461 ctacctttat atcctctaaa tatgtacaac ttaggcagaa gttaatcctc tgaaaagcag
     8521 caagcccaag ctcttgcttt ctaatgtaaa tcaataacta ttgatgactt aaggaaattt
     8581 gtaagttgcc aagtacaaaa actcagcagc tgaaatttaa acaaagagac catctctaaa
     8641 tagcatttct tgctctgatg gttctcatgt ggtataatta aaaaaaaata ttagtgtttt
     8701 ttatcattcc ttctctaaag tcatattggt cagcaactca ctgggaatgt gccatacttt
     8761 catcaggttg ggtctgttcc acctgtgttt ggacacacac atctctcatt caaaatggct
     8821 ttgttatcgt gaataaatac cttcatggtt acgaagatgt attgactgtt ttaaagatca
     8881 ggaaatcttc tctctgtttg acacacactt tgtaaaatga atgttttgtt tttgtttttg
     8941 ttttttgttt tttcaacaac atcatcctgt tatccccaga tgcagggaac attttgactt
     9001 cttctaaatc aggatgttta tgatctactt agtacttggt gatttcaaag aaacagattg
     9061 aatagttgag tttgaattct tggagttagc ctgataagaa aagagacaag caaagacaaa
     9121 gagatcctag aaagaccagg acataagaaa aaagaagaaa agtctttaaa ttttaagtga
     9181 gtgacgcttc caaataatgt gttgtggttt gtgaaaagga aaacatgaat cataaatcta
     9241 ttgagattaa gactaaagaa gacttacata gagtgaatag gttaaaactt ttgaaggata
     9301 taataaactt tctactaaac atatattttt aaaattttat gtgcatgggt gattttcctt
     9361 catgtatatc tatgtaaggg tgtcagattc actggaacta gtgttacaaa caattgtgag
     9421 ctaccatatg ggtactggga tttgaaccca ggtcccctgg aagagcagat agtgctctta
     9481 agcactgagc catctctcca gcacctaaaa gcataatcct tgatacatgt ccccaaatgt
     9541 ttggagaaac aagcaaaaat aaagataaaa atatttgtaa tttttttatt atctactcag
     9601 attggacctt gagactgtag atgcatgaga catttgtatt tcatccatat aaacaaagac
     9661 aagggtaaaa ttcccaagct agaatgaaca gaattccacc aaggtgaggc ttgactttca
     9721 atatttccgt attgtcatcg aaaaacactg acgttgtctg gaggttgcta tggatatgta
     9781 atatacatgt aagagtgtta ctttctaaac aaaagtgatg attataggat ttgatgtatt
     9841 ggatatgatt acattttatc tacattttta tattttctta tacttaacaa aatgtttttt
     9901 tttctctgag tgtcataaag tcctaaatac attgcataaa ttttcatgta gaagaagcat
     9961 attccagaga gatggtgtta ttttaagtac ttccttctgc cctagttctg gaaagctgcc
    10021 ttcatgatct tcaggctaaa acatttctat ctgtcctgaa aacactgcag ttcattttaa
    10081 gagcagcctt cctctccaaa atgacatgtc tcctcaaaaa cagaccttct gtttccatga
    10141 cttctctact tactctttat gactcttaat cttaaaatta gagttaataa acattggctt
    10201 cctcttttct cagagaagtt agaagccaga gaatgtcact caagaatgac aggtctaagc
    10261 cactcatttc tttagtaagc aatgcccata ttcctctatt tcttcggcaa atgatgccta
    10321 acatgttaga caacgggcat ggtactctat tctaggtcca gttttggggt caactaccat
    10381 actattatta acaagcttag aataaattta agcatataga agtattgaga tcatttttct
    10441 ttgctattta gtatattaaa gtattgtctg ttttctctac tttgtatgta tcaaaatagt
    10501 ggaatagtac ttctgggttt aactacatca ctaattaaat tgtactgcac aagtctgtca
    10561 atcaaggtgt atgatggtaa tttttcccag gctaatattt tggccaaatc tgggcccata
    10621 ctctaagact gaggaacatg gtatatccat ccaggttaaa cttcacctca ccttcactcc
    10681 tgacagaccc ctttcctctg ctttggtcct agcttgtaat tgtgaactct tgccattagc
    10741 cacccaccat gagtctggaa ttctaagcca gtgaccatat tttagaattt tgttcttata
    10801 cttatatagg attttttttc attctgatgg ctattaaaaa aaaacttatt tgtaaagatt
    10861 agaaaatgaa tgtctagtct tccatgaatc attcttcctt caaaataaag atcctcactc
    10921 acagaaatca aaaccaaaaa aaaaaaaaga caaaacaaaa taaaacaaac aaacaaacaa
    10981 aaaaccccaa ctaaatcatt taccatttta ccactttacc cactgttctt caaatgcatt
    11041 cacagcccta gtgtgttaaa cagtatggat gccatggttg tgcattaaca tgctttggta
    11101 tagcatactc aggccagcat ttccactgcc actttctcaa gagcccattt aataatgtct
    11161 aatggtgtgc cattggaata gacttagaac atctttaatt gagacagaga ctcactaata
    11221 atgctcctta aaattttcac atttgtgctg ttaatcaaat tctaccagat cttgatagcc
    11281 aaaacaatac acttgatcat ttcaaatttt caggatattt agaacttaga tctcagagta
    11341 atacaatcca ataccatgat aaaggtcacc tttcttttat atacacaatt agaaaacaaa
    11401 acaaaacaaa acaaaacaaa acaaaaaaac ccatacacac acacacacac acacacacac
    11461 acacacacac acacacacaa gtaaaccaaa ccaaaccaaa ccaaaataaa gaactactat
    11521 gcagaagaga agcatgctga aagcaaagac ctggagggga gcagccaagg caatgctgct
    11581 gcaaaaatga aggcccacag tgaattgatc agtgagtagc ttccagttct gtgtcacact
    11641 cacagtgaaa tcgtgctcag ctccctcaca acactttctg atgtgcttat gctctcactt
    11701 cttctatttt caatgaatgt tattataatg ccttgcaaat aagaaatgta catatcaata
    11761 ttaactagac ccaaagacta caactaaagg aactcaaata caattaaaaa ccgcccttcc
    11821 aagggaagct gtgagcatca cactgagttg ttcactttca ggactgagtc tctttaatca
    11881 catgcctatc actaacccat tccttagaag actgttgcaa ggtcctgttc tacatgcgga
    11941 gcactcatca gtgatggatg actgagcaaa acattggacc tgttctcatg ggagtagccc
    12001 ccagctaatt gtctttctgt ctacacacat aggccaagag agatgaccat attcatactt
    12061 tcatttacaa acttatttta tatcaaaatt gtccatgaca agagacttag gatgcttcat
    12121 tggattttta ctttgggatt tctatgctgc tggcattaaa tcatagatga taatatagga
    12181 tgaattttaa cactaatcac caggaataat taaactgttg atttcgacta attcagtgta
    12241 tgttttgtgc ttcttaatct gttgtcctgg cttgcctgaa actcacagag atcctcagcc
    12301 tcctaaagtc caattcaaca tattctggaa attggatggc ttaatatata aaatgaagag
    12361 gctgagagtg tagttaagtg atagagcaaa tactacgcaa ggaagccttg agttcaaacc
    12421 tgaaccaaag agagagaaag agggtggggg gagagagaga gagacctctc tagtagggga
    12481 tctctataac aagtttgtaa gaaagatagt tactaaattg actctcaaat tgatccttaa
    12541 ccaatgtgta gttgcccaca tctgtaattc cagcactcaa gaagaggttt tgattttcat
    12601 agtaacaccc tgttttagaa gagaaaaatg aatactcagc aattaatgcc attctaatga
    12661 caatggcaat aacttgctcc tgtacagaca tgacatagtt acaattcaag gacagccaag
    12721 atcatcaagc tccacaaaac aatgaccagc atctcatctg ccatgtcagg aaaccctcta
    12781 cagccattgt tcatatagca gcatagtcca aagagagagt gacaaatgga gtctagcagt
    12841 gacatcaaag ccaaactcag aggaggatag aattttctat gtgtgtccta gaggttctgc
    12901 caatgaagaa accctttaga gaaatttatc catatgtgga aaaataccac tgccatactg
    12961 catataaaaa aatttaatcc tagatgaatt gaaaaatcca aacttgaaac gttaccataa
    13021 aagactttaa aatacataaa aatatcgtcc tgttattaaa gtagagtcaa aaattagtaa
    13081 acaatacaca tgttcatctg taacagtaaa gaagaataag tcaaaattta aatctctgta
    13141 tatcagataa tggttacaaa atgtcaaata gcacattgca aaagtcagtg gttttttata
    13201 tatagaaatt taatacacta acaattacta agaatataat tcaatttaaa ccagaatatt
    13261 taggagctgt tcaacataac taaaataaaa aaaaatacaa tgcagattaa attaaaggaa
    13321 agaatccttt catggtggta gagtttgtct gaataaacaa aacacaagat ttaaaggaaa
    13381 aagttcaaaa atatagaaag gaaagaagaa agtgaggcaa ggagtgagaa aggaagttat
    13441 ggaggattaa ggaaggagga aaaggaagaa gagaagaaag gaaacagaat tgaaacatgt
    13501 gacaaaaacc tagaaaaaga ataaatatat gggggttaaa tcctcagaca gaaaccctaa
    13561 gtggaagcag agaactccat caataaaaac actattccat caagagtact agatattgaa
    13621 gcatcagact tcagaagtaa agctttaagc cgggagaaat tgaaatagca aatgccaaga
    13681 ggaaatgtgg tatgagaagg gggtttttag gttgaaccaa aaacttacat cgcaaataca
    13741 acagcattca agagacccct gtcaaaagta aggaatttat aaatgttatt tccatgggca
    13801 ttttctgaga cactggctag aaaatgacca gcagagagca aagaccaaat gagcattaga
    13861 atgtcacatg tacagagttt gtgactgtag aagttgacca tttggattca gcctaaagtt
    13921 aagaactatg gatacaataa agaagtcatg tttgcataga atacagtaga gaaagcaaac
    13981 ggttaagtta cttataatag ttatatttgc attggcaact cttgtgtttt taatgtaaaa
    14041 atatcttgct ataatttcct tataattctg ttactaaaca aaatacaaaa cagaacagca
    14101 acaataacaa taacaacaac aaagccgcag cattatctgc tttgcagaaa ctactcacta
    14161 aactataaag aaaaccaaga tggtttatca gctccagaga agtgtctgtg catcaaaaag
    14221 gaaaagagtt atgactgaaa tgaggcaggt actgtaaaac aatccttgtt aatctgggtc
    14281 tcctctaccc tcaaagtatt tccttcttaa catgctatgt tgcaccaaaa cacttacttc
    14341 atagagttgt tctgtacttg tattatattt ttaataaact attgcaacta gagtaaaaga
    14401 aaaaatcacg cgaaaatgtt ttcaactgaa agccagttta tgaacagaaa gggtgcaccc
    14461 actgtcaggg ctcagggtca actatgagga aaaagtatcc aatgaggatg tcaggacaac
    14521 aaaagtagag caacaacatc actaggtccc taggtaagat ctcgagggaa cacattgaag
    14581 aaatgcactc gtggtactaa gaaaaaaatt atgaccaact cagaatttcc tatagaacaa
    14641 acaggattgc agggtatcag atcatgaagg gatgaacact tggggaagat tattgaaaca
    14701 aacaaataaa caaacaacag caacaacaat aacaacaaca aaaaacaaac aagactggtt
    14761 caagggctag gagagagctc aggtgtggag attgtaagcc aagacaacag cattaaagta
    14821 ataaactagc tgatgttttt caaccagtag aaaatatatt catagacttt ttccctataa
    14881 ggagttataa aactgtaacc aaatggtaat tatcaatgca gaaaaaaaag gacttcaaca
    14941 aatggaattt aatcttgttt ctacaagtga aggacagtgc acttacaggt tgacttatca
    15001 atgggggaaa tcaggtatca cactgttttc tggtcaactt ccaagaacgt tgttccaatt
    15061 tccattccca cattttagag aacctgtttc tcttttctca agctgataat gtgataatca
    15121 atttttatct ttttctttaa aataaaaatg gaacttagtt attgtattaa tttgcatttt
    15181 aaatggatgc cctgcatctt ttcttgtggt tataagtcct tgcatttatt tttttatatt
    15241 tgctgtactt ctttactttg tatatatttt ctatttgttt atatttctct tcttagtaat
    15301 tagaggcact ctgtcaataa ataaagtaag tggtttttgt ca
//
