LOCUS       NM_001360340            3687 bp    mRNA    linear   ROD 17-JUL-2024
DEFINITION  Mus musculus NKAP domain containing 1 (Nkapd1), transcript variant
            3, mRNA.
ACCESSION   NM_001360340 XM_011242545
VERSION     NM_001360340.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Nakai,R., Yokota,T., Tokunaga,M., Takaishi,M., Yokomizo,T.,
            Sudo,T., Shi,H., Yasumizu,Y., Okuzaki,D., Kokubu,C., Tanaka,S.,
            Takaoka,K., Yamanishi,A., Yoshida,J., Watanabe,H., Kondoh,G.,
            Horie,K., Hosen,N., Sano,S. and Takeda,J.
  TITLE     A newly identified gene Ahed plays essential roles in murine
            haematopoiesis
  JOURNAL   Nat Commun 15 (1), 5090 (2024)
   PUBMED   38918373
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3687)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC025500.8.
            
            On Aug 24, 2022 this sequence version replaced NM_001360340.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253013.2673451.1,
                                           SRR6116558.145475.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-610               AC025500.8         80941-81550         c
            611-687             AC025500.8         79766-79842         c
            688-788             AC025500.8         76660-76760         c
            789-938             AC025500.8         74583-74732         c
            939-992             AC025500.8         73655-73708         c
            993-3687            AC025500.8         69525-72219         c
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 27.75 cM"
     gene            1..3687
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /note="NKAP domain containing 1"
                     /db_xref="GeneID:270156"
                     /db_xref="MGI:MGI:2143205"
     exon            1..610
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /inference="alignment:Splign:2.1.0"
     exon            611..687
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /inference="alignment:Splign:2.1.0"
     CDS             619..1497
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /note="isoform 1 is encoded by transcript variant 3"
                     /codon_start=1
                     /product="uncharacterized protein NKAPD1 isoform 1"
                     /protein_id="NP_001347269.1"
                     /db_xref="CCDS:CCDS23166.2"
                     /db_xref="GeneID:270156"
                     /db_xref="MGI:MGI:2143205"
                     /translation="MSRVPLGKVLLRNVIRHTDAHNKIQEESDMWKIRELEKQMEDAY
                     QGTRRNTVPSSSSRMRSDGFDEESQRDYWRSKNEISGALEDDFLKTKSWNKKLYDYES
                     NIPDRWGHSGYKELYPEEFETDSSDQQDITNGKKPSPQVKSSARESRKHKKSKKSHKK
                     KQKKRSHKKQKKNKKEVTDITADSSSEFSEETRASSTRKRKQPHKSKKKSRKKSPKIT
                     FPLGGESGTSQSEDSASSSSEDIEERDTKKTKRKKKEKSVHVPMPDPEVQERTSKRRN
                     WKVATDARSAESSEDD"
     exon            688..788
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /inference="alignment:Splign:2.1.0"
     exon            789..938
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /inference="alignment:Splign:2.1.0"
     exon            939..992
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /inference="alignment:Splign:2.1.0"
     exon            993..3687
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3416..3421
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /note="hexamer: AATAAA"
     polyA_site      3437
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /note="major polyA site"
     regulatory      3668..3673
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /note="hexamer: AATAAA"
     polyA_site      3687
                     /gene="Nkapd1"
                     /gene_synonym="Ahed; Gm639"
                     /note="major polyA site"
ORIGIN      
        1 accttttctc ccaccccgcc acgcgaggct gcggcgcacg gtatgggtgt gtgtgtgtgt
       61 gtgtgtttgt gtcgggaggg cgttgcgagg gaaggccgcc gagagcgccg gggctgcggc
      121 ggggcaggga gcccgctgac agacatgggg gcgctttccc ccacgtcccc ttggaaacct
      181 caagctcccc ccgaggcact ctagatactt cctggggccc aacggaaggc cgtgcccaag
      241 ccctgtgtcc ccattctgtc ccgggagtga aagcgagccg ccgggcgccg gcccagccca
      301 gcgagagcgg ggaatgcccc ggtccgcccg cccgcaggcc gctttccgcg cctgcgcctc
      361 ctctggcctg ggtccccgag agcgagccgc cgggcgggct cggctggggg aaggcagccg
      421 tagcctgggc ctcgggtgag gagggcacag taaccggtgg gaaggctgcg ttttcacgaa
      481 ggactcgggt gaagctgcag agctgccttt cagccctgac tccttggctt cctgggtcgg
      541 aggagatctt gtaatggagt ggttcttcgt ctcacactaa caggatgcct gatttcctca
      601 ggatcgaggg attaaagaat gtcccgggtt ccgttgggga aagtcctctt gaggaatgtc
      661 atccggcaca cagatgctca caataagatt caggaggagt ctgacatgtg gaaaatcaga
      721 gaactagaga agcagatgga ggatgcttac caggggacca gaaggaacac agtgcccagc
      781 agctcaagtc ggatgagaag tgatggtttt gatgaagaaa gtcagagaga ttactggagg
      841 tcaaagaatg aaatttctgg ggccttggaa gatgattttc ttaagactaa gtcctggaac
      901 aagaagttat atgattatga atccaatata ccagacagat ggggtcacag tggttataaa
      961 gagttgtatc ctgaagagtt tgaaacagac agtagtgatc agcaagacat taccaatgga
     1021 aaaaaaccat ctccccaggt aaaatcatct gcccgtgaat ctcgaaaaca taagaagtca
     1081 aagaaatccc ataagaagaa gcagaaaaaa cggtcacaca aaaaacagaa gaaaaacaaa
     1141 aaggaagtta cggacataac agcagattcc tcaagtgagt tctcagagga aactcgagct
     1201 tctagtacta ggaaaaggaa gcaaccacac aagagcaaga aaaagtccag gaaaaagtct
     1261 cctaaaatca ctttcccttt agggggagaa agtggtactt ctcagtcaga ggattctgca
     1321 tctagcagtt ctgaggacat cgaggaaaga gatactaaga aaaccaaacg gaaaaagaaa
     1381 gagaagagtg tccatgtccc catgcctgac cctgaggtac aggagaggac aagcaagcgc
     1441 aggaactgga aggtggctac cgacgccagg tctgctgaga gctcggagga tgactaagtg
     1501 ggagagcagc agtcctgctt cccactgact cgttaggggg gttgccagtg actcctgcag
     1561 cacaggcagg cgtctgaggt cagagttgtc ctgccccaga tacctttttt cctattttgg
     1621 cttgttaatc tccatcctct tttgttaata gggaatctgg tattttatta aggtttcttg
     1681 aaaaaattat ttttttcaat taatcactta gggtagaata cctatacagc aaattaaaga
     1741 acccagaaag ctgaactcag taatgacctg ggcacaccaa ttgcaatgtt ggatttgatg
     1801 gaatcaagag tccaggatgg aggaggaaga ctgaagtagt gctaaataga atggtttgac
     1861 aaggagaccg tgctattcat acaggaggca tgctgccttc agctttggtg ctttggttct
     1921 tctgcagttg ggccctatag gcatccagtt tactgagcga ggaaatagtt aaaagaacaa
     1981 accttttccc ttcatggtaa tctctacaga tgactgctaa ggaacctagg gttttcagcc
     2041 taatctccct gctggccagg gtctacagct acatttagca taacccaaac ataacagtaa
     2101 ctggatagta aatgtttttc tagttccatt gctgtatact gcctaaatgg gcttgtattt
     2161 gaaattgttt ggttgtccta tctatcaaat ggaaagaatt aggaagtagt tatgttgcca
     2221 tgatgctttc aaagagattc agggcatcag ccatctaaat gtctgttttt taaaaaaatt
     2281 ttattattat tattattatt attattattt ttgctgagtt ggggattgaa ccgaaggcct
     2341 tgcatatact aggcaagtgc tctaccattg agccataccc ccagtcctgt tgttttcctt
     2401 gttcatttct aattctatta atggatttta gattttccct gaaaccagtt gaatcaattc
     2461 caaaatgaaa cacacttctc agggacatac ccacttccca gtctgacttt gtgttttgat
     2521 tatcaagaac atattatttc cccctgctat atactgtgat cctgatatta aaagaaacca
     2581 aaataccact ggaaagtggc tggtagaaca gttggctttt cattaagagg aaagataaag
     2641 tgacatttat cagggttttt gtttttgagg ttgtcacttt ggtactgagc gaatgcatga
     2701 aaactcaaga ctgtgggcca tggttctgcc actaattgat tgagttctag agcaggttaa
     2761 cctccctggc cttactcttt gcatgtgtaa tacaaagata cttcatggtt agcatgacat
     2821 acaccagcaa tagaatatga taatgctgac atcccatgaa gtggcatttt atagtaataa
     2881 ctaaatttgt gctctttatg gaacatattt ctaataaaat gcttgttctt aaaatatatg
     2941 gttggccaga tgtgccctat cccttgatta tgagcaggtg ccgccttttg gggtactgaa
     3001 acactaatga ttttagtact caaatgccaa tattccatga gccttttggg gtactgaaac
     3061 actaatgatt ttagtactca aatgccaata ttccatgata tattcttttc cttgggtcta
     3121 gtggagggag agtctagtta ctacctcata accttctaga agcaagtgat atctttgttg
     3181 gaagtagttt tgggcatgca ttcttaatgc agttttgtgc tacaaatgta ctgattctaa
     3241 gcacagactc gggaagattg tcctgcagaa taaggcactt ccaagtttga gttttgacct
     3301 taccttcctc ccatacaaca gacatatctt ttctccaggg aaagcacatt ttgcctgatt
     3361 taacacccct gccccaccac cccgcactgt tctatctttt cttggtcaca taagcaataa
     3421 aattgtttct gttctaatcc atgccttagg ctaaggcttt gatggttcct ctaatcccct
     3481 taacagtggt ttaggactac ttttgaaaac ctgtctctcc catttccacg atcagctctg
     3541 gaacatgtat tagtgttcct gagagatgat tatgttgaat cctaaattgg gatttagaag
     3601 aacaagaatt ggagacttca catttattaa ctggtgtgat acaagttgga attactgaat
     3661 tcccataaat aaaatttcac ttttgaa
//
