LOCUS       NM_025937               4262 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus NFKB activating protein (Nkap), mRNA.
ACCESSION   NM_025937
VERSION     NM_025937.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Shapiro,M.J., Anderson,J., Lehrke,M.J., Chen,M., Nelson Holte,M.
            and Shapiro,V.S.
  TITLE     NKAP Regulates Senescence and Cell Death Pathways in Hematopoietic
            Progenitors
  JOURNAL   Front Cell Dev Biol 7, 214 (2019)
   PUBMED   31632967
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Dash,B., Shapiro,M.J., Thapa,P., Romero Arocha,S., Chung,J.Y.,
            Schwab,A.D., McCue,S.A., Rajcula,M.J. and Shapiro,V.S.
  TITLE     The Interaction between NKAP and HDAC3 Is Critical for T Cell
            Maturation
  JOURNAL   Immunohorizons 3 (8), 352-367 (2019)
   PUBMED   31387873
  REMARK    GeneRIF: these results demonstrate the significance of NKAP-HDAC3
            association in T cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4262)
  AUTHORS   Dash,B., Belmonte,P.J., Fine,S.R., Shapiro,M.J., Chung,J.Y.,
            Schwab,A.D., McCue,S.A., Rajcula,M.J. and Shapiro,V.S.
  TITLE     Murine T Cell Maturation Entails Protection from MBL2, but
            Complement Proteins Do Not Drive Clearance of Cells That Fail
            Maturation in the Absence of NKAP
  JOURNAL   J Immunol 203 (2), 408-417 (2019)
   PUBMED   31175160
  REMARK    GeneRIF: NKAP-deficient immature peripheral T cells remain scarce
            in complement-deficient mice likely due to ferroptosis.
REFERENCE   4  (bases 1 to 4262)
  AUTHORS   Shapiro,M.J., Lehrke,M.J., Chung,J.Y., Romero Arocha,S. and
            Shapiro,V.S.
  TITLE     NKAP Must Associate with HDAC3 to Regulate Hematopoietic Stem Cell
            Maintenance and Survival
  JOURNAL   J Immunol 202 (8), 2287-2295 (2019)
   PUBMED   30804042
  REMARK    GeneRIF: Y352 in NKAP is critical for association with HDAC3 and
            for HSC maintenance and survival but is not important for
            proliferation of murine embryonic fibroblasts, demonstrating that
            NKAP functions in different complexes in different cell types.
REFERENCE   5  (bases 1 to 4262)
  AUTHORS   Dash,B., Shapiro,M.J., Chung,J.Y., Romero Arocha,S. and
            Shapiro,V.S.
  TITLE     Treg-specific deletion of NKAP results in severe, systemic
            autoimmunity due to peripheral loss of Tregs
  JOURNAL   J Autoimmun 89, 139-148 (2018)
   PUBMED   29366602
  REMARK    GeneRIF: this study shows that Treg-specific deletion of NKAP
            results in severe, systemic autoimmunity due to peripheral loss of
            Tregs
REFERENCE   6  (bases 1 to 4262)
  AUTHORS   Thapa,P., Das,J., McWilliams,D., Shapiro,M., Sundsbak,R.,
            Nelson-Holte,M., Tangen,S., Anderson,J., Desiderio,S., Hiebert,S.,
            Sant'angelo,D.B. and Shapiro,V.S.
  TITLE     The transcriptional repressor NKAP is required for the development
            of iNKT cells
  JOURNAL   Nat Commun 4, 1582 (2013)
   PUBMED   23481390
  REMARK    GeneRIF: Transcriptional repressor NKAP is required for invariant
            natural killer T cell but not conventional T cell development.
REFERENCE   7  (bases 1 to 4262)
  AUTHORS   Hsu,F.C., Pajerowski,A.G., Nelson-Holte,M., Sundsbak,R. and
            Shapiro,V.S.
  TITLE     NKAP is required for T cell maturation and acquisition of
            functional competency
  JOURNAL   J Exp Med 208 (6), 1291-1304 (2011)
   PUBMED   21624937
  REMARK    GeneRIF: NKAP is required for T cell maturation and the acquisition
            of functional competency
REFERENCE   8  (bases 1 to 4262)
  AUTHORS   Pajerowski,A.G., Shapiro,M.J., Gwin,K., Sundsbak,R.,
            Nelson-Holte,M., Medina,K. and Shapiro,V.S.
  TITLE     Adult hematopoietic stem cells require NKAP for maintenance and
            survival
  JOURNAL   Blood 116 (15), 2684-2693 (2010)
   PUBMED   20610818
  REMARK    GeneRIF: Inducible deletion of NKAP in adult mice leads to
            lethality within 2 weeks, at which point hematopoiesis in the bone
            marrow has halted and hematopoietic stem cells have disappeared.
REFERENCE   9  (bases 1 to 4262)
  AUTHORS   Pajerowski,A.G., Nguyen,C., Aghajanian,H., Shapiro,M.J. and
            Shapiro,V.S.
  TITLE     NKAP is a transcriptional repressor of notch signaling and is
            required for T cell development
  JOURNAL   Immunity 30 (5), 696-707 (2009)
   PUBMED   19409814
  REMARK    GeneRIF: NKAP functions as a transcriptional repressor, acting on
            Notch target genes, and is required for alphabeta T cell
            development.
REFERENCE   10 (bases 1 to 4262)
  AUTHORS   Chen,D., Li,Z., Yang,Q., Zhang,J., Zhai,Z. and Shu,H.B.
  TITLE     Identification of a nuclear protein that promotes NF-kappaB
            activation
  JOURNAL   Biochem Biophys Res Commun 310 (3), 720-724 (2003)
   PUBMED   14550261
  REMARK    GeneRIF: NKAP is a novel nuclear regulator of TNF- and IL-1-induced
            NF-kappaB activation
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ150721.1, AK011492.1, AK038344.1 and AL451076.14.
            
            On Sep 3, 2009 this sequence version replaced NM_025937.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK038344.1, AK089943.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                CJ150721.1         2-50
            50-1798             AK011492.1         1-1749
            1799-2366           AK038344.1         1747-2314
            2367-2367           AL451076.14        75401-75401
            2368-3945           AK038344.1         2316-3893
            3946-4262           AL451076.14        76980-77296
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 21.54 cM"
     gene            1..4262
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /note="NFKB activating protein"
                     /db_xref="GeneID:67050"
                     /db_xref="MGI:MGI:1914300"
     exon            1..613
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             234..1481
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /note="nuclear NF-kappaB activating protein"
                     /codon_start=1
                     /product="NF-kappa-B-activating protein"
                     /protein_id="NP_080213.3"
                     /db_xref="CCDS:CCDS30069.1"
                     /db_xref="GeneID:67050"
                     /db_xref="MGI:MGI:1914300"
                     /translation="MAPVSGSRSPEREASGAKRRSPSRSPKSIKSSRSPRCRRSRSRS
                     CSRFGDRNGLSHSLSGFSQSSRNQSYRSRSRSRSRERPSAQRSAPFASASSSAYYGGY
                     SRPYGGDKPWPSLLDKEREESLRQKRLSERERIGELGAPEVWGLSPKNPEPDSDEHTP
                     VEDEEPKKSTTSASSSEDDKKKKRKSSHSKDRAKKKRKKKSSKRKHKKYSEDSDSDSE
                     SDTDSSDEDSKRRAKKAKKKDKKKKRRGKKYKKKKSKKNRKESSDSSSKESQEEFLEN
                     PWKDRSKAEEPSDLIGPEAPKTLASQDDKPLNYGHALLPGEGAAMAEYVKAGKRIPRR
                     GEIGLTSEEIASFECSGYVMSGSRHRRMEAVRLRKENQIYSADEKRALASFNQEERRK
                     RENKILASFREMVYRKTKGKDDK"
     exon            614..694
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            695..765
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            766..906
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            907..970
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1080
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1156
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1306
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..4262
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4186..4191
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4203
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
     regulatory      4234..4239
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4254
                     /gene="Nkap"
                     /gene_synonym="2610020O08Rik"
                     /note="major polyA site"
ORIGIN      
        1 agaggtacac aaatcccttc ctgttggcgg caaggactct gggtccgggg gaaggggttt
       61 accgctgtac acggaactgt ttccaagttg gtttttttgt ttgtttgttt gtttgcttgc
      121 tttttttcgg atttggtcgg ctgcaagctc taggacccta ggtgtccggg acctttgggg
      181 gtcgcgagag cgcctccttg tcccgcccct tggtagcttc agcttccccc accatggctc
      241 ctgtatcggg ctcgcgtagc cctgaacggg aggcctccgg ggcgaagcgt cgcagtccat
      301 cgcggagccc caaatccatc aaatcttccc gctccccgcg gtgtcgccgg tctcgctcgc
      361 gctcttgttc tcggttcggc gaccggaatg gcctcagtca ttcgctgagt ggcttcagcc
      421 aaagctcccg aaaccaatct taccgctccc gctctcgatc gcgctctcgc gaacggccct
      481 cggcacagcg gagcgccccg tttgcttctg catcctcgtc cgcctattac ggcggctact
      541 cgcgtcccta cgggggcgac aagccatggc ctagcctgct ggacaaggaa agggaggaga
      601 gtctccggca gaagagatta agtgaaagag agaggattgg agaattggga gctcctgaag
      661 tttggggact ttccccaaag aatcctgaac cagactctga tgaacataca ccagtagagg
      721 atgaagaacc aaagaaaagc actacttcag catctagttc agaagatgat aagaagaaga
      781 aaaggaaatc tagtcattca aaagacagag ccaagaaaaa gagaaagaaa aagtcatcta
      841 aaagaaaaca taaaaagtat tctgaagata gcgacagtga ctctgaatct gacacagact
      901 ccagcgatga agattcaaaa agaagagcaa agaaagctaa gaaaaaggac aagaaaaaga
      961 aacgaagagg gaagaaatac aagaaaaaga agtctaagaa aaacagaaaa gagtccagtg
     1021 attcgagctc taaagagtcc caagaagaat ttctagagaa tccttggaag gatcgatcaa
     1081 aggcagaaga accatcagat ttgattggcc cagaggctcc caaaactctt gcctcccaag
     1141 atgataagcc tttgaactat ggccacgctc tgttacctgg tgaaggcgca gctatggctg
     1201 aatatgtaaa agctggcaag cgtatcccaa gaagaggtga aattggcttg acaagtgaag
     1261 agatcgcatc atttgaatgt tccggttacg taatgagtgg tagcaggcat cgccgaatgg
     1321 aggctgttcg acttcggaaa gagaaccaga tctacagtgc tgatgagaag agagcccttg
     1381 catcctttaa ccaagaagaa agacgaaaga gggagaacaa gattctggcc agctttcgtg
     1441 agatggtata cagaaagacc aaagggaagg atgacaagtg aggacttctt ggctttacaa
     1501 gtgaatattt ttaacatcga aaaggaaatt gtgggttcta acaacaaaag cttattgttt
     1561 tgtttttgtt ttgagttact gtttgatgta tggtttcaat tagcccaggt tgacctcagg
     1621 ctttgtagcc aaggatgact ctgaactctt ggtctcctgc ctccaccttc caagtgctga
     1681 aattcgaggt gtgtgccacc aggcccagcc aaaaaggcta ctgtgctatg aagtggcttt
     1741 tcagggcttc tgtgccttct ttttaattct tcagtccttc aataaaagat gcttgttgat
     1801 aaaattagtt aagttatttg aggcttttaa agattgaccc cagccttctg gtttaaaagt
     1861 ccaaagtcct gctatgttcg gaaggtggca tagagagaaa gtggcagatg aacgagaagg
     1921 ttaagtctca catcatgatc tggtgtgact atgattatta gccccataca gtgtctgcca
     1981 ctatcccagc agaacttgag aatttcatat cttgttttga atgtgttaca gatgggcttg
     2041 acagaaacta tttaggaggc tatttctgtt aacaaactga cggtgttgct gtgcacagcc
     2101 actctggaat tggaaaactg gcttgcctct atagtcggcc ctagaaatgt atactgggct
     2161 aactctacct ctgctctaaa accaagcaaa ccaaatggct gactcacaca tgtgtggggc
     2221 agaaaatgtg agcattagct gtttcccgtc ctattacatt acttaccatt gttctttcag
     2281 tggctaggat gtagctcaga gttagagcac gtgcttatga ggccccgagc tacatcccca
     2341 gcagtgcaaa aaagttccaa atgcagtttt gggagtctat tgtgccaata taagaatact
     2401 atttctattt aagctggtat ttaatagagg ataatatctt aaaaatgagt attaagagtg
     2461 ctaataaggg aaaatataat aagtcagtat tttaattaag ctgtttcatg atgatgatga
     2521 aagtaaaata ttaaacattt tcaattatgt cctttatatt accagatttc ttgtttgaga
     2581 cagagtctca ctatgtagcc ctggatggcc tagaactccc agagttcccc ctgcctcagc
     2641 ctgccaggtg ctaggattag aggtgtgtgc cactgcacct cactatagga ttttatttta
     2701 aatattgtta agctcttcag ctttttatat ctgtcatgac tttaatcctt aactttctag
     2761 aaaccctcct tacttcatta ctgtttgaaa gtttaatata tgttctgaga aaatgtgttt
     2821 ctaacccaca actgctgaac taagcagcag ttagcatgta gtaaaatctg tgatctttga
     2881 acttgaaatt atttcatggt atttatacgt ctcagtctga taagtatata gtttcctaaa
     2941 gtctaatgga aactaggttc caaagttgaa agtcttctta atccatacac ttcctcatgt
     3001 aatacaatgt aaagtttata atcgctactt taaaagttaa aacgaagggc cattattttt
     3061 tgttaaatgg ctcctagcaa ctgggtgaat atgtttttct tttttttaat aggaaatgta
     3121 gtttcaatgc aacaattttg tagtgtataa gtattacaga ttttcttttg attattctcc
     3181 acagagtata tacagtcaat gaaaaaatag gaatttattt aaaaatctat cattatttag
     3241 tagcagtcct ttaaaaaatg gagggctaaa agtattttat ttttagcttg tttgaattat
     3301 cccttcaaac tgtgttcttt cacctgagct ctaagtcagc cattgtttgc aaaaacatat
     3361 ctttccttgt ctacagaaga agaaacacac actcgtgtac acacacacac acacacacac
     3421 acacacacac acacttataa ttgaacaaaa cattaagcca ggaatttcct ctttgttaca
     3481 ttcctagtga acaaggtagt ggttatgctt ctatctgttt tctttatagc tctttaggcc
     3541 tgagtaaagg ctatgtagaa gtattgtaat ttaaatattt tgttttctaa agagacttac
     3601 tcagtttctc acctcttcgc ctgttgttta taggatttct ctcttttttt atttgttgta
     3661 caaagataat aggaaaaata gagaaattgc tcagtcctac cacatgaatt gttacaagtg
     3721 ttctgtgtcc cttgtacgtc tttgtcattt gaagtgtact gtaaagttgt ctttggtatt
     3781 ttaaaattgt gtgagcaggc ataggagtcc cattgggaag ttaggaatac atgatgatcc
     3841 agtgtgaaga cagaaataag gaagggtggt aacccacccc tgcttcattg caatcatgag
     3901 catgatggga tgttcagagt ttagcagtga ggtcatctga cagtagaaga gatctatggg
     3961 tagtggagcc cttcagactg ttagtgtgag atatagaacg gttgccagtt tgttatccag
     4021 atacccataa aaatgtctat caagcctaga gctaggcaga gagaaggacc agacagctaa
     4081 gggccagtgg ctgaacttta gtaatgttca caactcgact ctgtgcttgc cacactccaa
     4141 aatcttctcc agaacagaaa acaacttatt gcagaatgga taatgaataa aaacaaaaac
     4201 acagttgggt tgtgtgccat gagtttatta ctcaataaat attatgtgct ttgacatgtt
     4261 tc
//
