LOCUS       NM_012049               1342 bp    mRNA    linear   ROD 23-DEC-2022
DEFINITION  Mus musculus nitrilase 1 (Nit1), transcript variant 1, mRNA.
ACCESSION   NM_012049
VERSION     NM_012049.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Peracchi A, Veiga-da-Cunha M, Kuhara T, Ellens KW, Paczia N,
            Stroobant V, Seliga AK, Marlaire S, Jaisson S, Bommer GT, Sun J,
            Huebner K, Linster CL, Cooper AJL and Van Schaftingen E.
  TITLE     Nit1 is a metabolite repair enzyme that hydrolyzes deaminated
            glutathione
  JOURNAL   Proc Natl Acad Sci U S A 114 (16), E3233-E3242 (2017)
   PUBMED   28373563
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Wang YA, Sun Y, Le Blanc JM, Solomides C, Zhan T and Lu B.
  TITLE     Nitrilase 1 modulates lung tumor progression in vitro and in vivo
  JOURNAL   Oncotarget 7 (16), 21381-21392 (2016)
   PUBMED   26967383
  REMARK    GeneRIF: Data show that cisplatin response was enhanced in human
            lung cancer cells when Nit1 was knocked down and mice
            Nit1-/-:KrasG12D/+ tumors showed increased sensitivity to cisplatin
            in vivo.
REFERENCE   3  (bases 1 to 1342)
  AUTHORS   Sun J, Liu J, Pan X, Quimby D, Zanesi N, Druck T, Pfeifer GP, Croce
            CM, Fong LY and Huebner K.
  TITLE     Effect of zinc supplementation on
            N-nitrosomethylbenzylamine-induced forestomach tumor development
            and progression in tumor suppressor-deficient mouse strains
  JOURNAL   Carcinogenesis 32 (3), 351-358 (2011)
   PUBMED   21097531
REFERENCE   4  (bases 1 to 1342)
  AUTHORS   Sun J, Okumura H, Yearsley M, Frankel W, Fong LY, Druck T and
            Huebner K.
  TITLE     Nit1 and Fhit tumor suppressor activities are additive
  JOURNAL   J Cell Biochem 107 (6), 1097-1106 (2009)
   PUBMED   19479888
  REMARK    GeneRIF: the extent of tumor susceptibility due to Nit1 and Fhit
            deficiency is additive
REFERENCE   5  (bases 1 to 1342)
  AUTHORS   Zhang H, Hou YJ, Han SY, Zhang EC, Huebner K and Zhang J.
  TITLE     Mammalian nitrilase 1 homologue Nit1 is a negative regulator in T
            cells
  JOURNAL   Int Immunol 21 (6), 691-703 (2009)
   PUBMED   19395373
  REMARK    GeneRIF: genetic deficiency results in T cell hyperproliferative
            responses induced by TCR stimulation, spontaneous cell cycle entry
            and enhanced cell cycle progression
REFERENCE   6  (bases 1 to 1342)
  AUTHORS   Barglow KT, Saikatendu KS, Bracey MH, Huey R, Morris GM, Olson AJ,
            Stevens RC and Cravatt BF.
  TITLE     Functional proteomic and structural insights into molecular
            recognition in the nitrilase family enzymes
  JOURNAL   Biochemistry 47 (51), 13514-13523 (2008)
   PUBMED   19053248
REFERENCE   7  (bases 1 to 1342)
  AUTHORS   Semba S, Han SY, Qin HR, McCorkell KA, Iliopoulos D, Pekarsky Y,
            Druck T, Trapasso F, Croce CM and Huebner K.
  TITLE     Biological functions of mammalian Nit1, the counterpart of the
            invertebrate NitFhit Rosetta stone protein, a possible tumor
            suppressor
  JOURNAL   J Biol Chem 281 (38), 28244-28253 (2006)
   PUBMED   16864578
  REMARK    GeneRIF: Nit1 and Fhit share tumor suppressor signaling pathways,
            while localization of the NIT1 gene at a stable chromosome site
            explains the paucity of gene alterations and in frequent loss of
            expression of the NIT1 gene in human malignancies.
REFERENCE   8  (bases 1 to 1342)
  AUTHORS   Granjeaud S, Naquet P and Galland F.
  TITLE     An ESTs description of the new Vanin gene family conserved from fly
            to human
  JOURNAL   Immunogenetics 49 (11-12), 964-972 (1999)
   PUBMED   10501839
REFERENCE   9  (bases 1 to 1342)
  AUTHORS   Eddleston J, Murdoch JN, Copp AJ and Stanier P.
  TITLE     Physical and transcriptional map of a 3-Mb region of mouse
            chromosome 1 containing the gene for the neural tube defect mutant
            loop-tail (Lp)
  JOURNAL   Genomics 56 (2), 149-159 (1999)
   PUBMED   10051400
REFERENCE   10 (bases 1 to 1342)
  AUTHORS   Pekarsky Y, Campiglio M, Siprashvili Z, Druck T, Sedkov Y, Tillib
            S, Draganescu A, Wermuth P, Rothman JH, Huebner K, Buchberg AM,
            Mazo A, Brenner C and Croce CM.
  TITLE     Nitrilase and Fhit homologs are encoded as fusion proteins in
            Drosophila melanogaster and Caenorhabditis elegans
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8744-8749 (1998)
   PUBMED   9671749
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC087229.27.
            
            [WARNING] On May 5, 2023 this sequence was replaced by NM_012049.3.
            
            On Jun 15, 2010 this sequence version replaced NM_012049.1.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF069988.1, BC021634.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                AC087229.27        90940-91017         c
            79-165              AC087229.27        90286-90372         c
            166-417             AC087229.27        89660-89911         c
            418-521             AC087229.27        89436-89539         c
            522-655             AC087229.27        89066-89199         c
            656-781             AC087229.27        88810-88935         c
            782-1342            AC087229.27        87609-88169         c
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 79.35 cM"
     gene            1..1342
                     /gene="Nit1"
                     /gene_synonym="ESTM30"
                     /note="nitrilase 1"
                     /db_xref="GeneID:27045"
                     /db_xref="MGI:MGI:1350916"
     exon            1..78
                     /gene="Nit1"
                     /gene_synonym="ESTM30"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..1048
                     /gene="Nit1"
                     /gene_synonym="ESTM30"
                     /EC_number="3.5.1.128"
                     /note="isoform 1 is encoded by transcript variant 1;
                     nitrilase homolog 1; dGSH amidase; deaminated glutathione
                     amidase"
                     /codon_start=1
                     /product="deaminated glutathione amidase isoform 1"
                     /protein_id="NP_036179.1"
                     /db_xref="CCDS:CCDS35775.1"
                     /db_xref="GeneID:27045"
                     /db_xref="MGI:MGI:1350916"
                     /translation="MLGFITRPPHQLLCTGYRLLRTPVLCTQPRPRTMSSSTSWELPL
                     VAVCQVTSTPNKQENFKTCAELVQEAARLGACLAFLPEAFDFIARNPAETLLLSEPLN
                     GDLLGQYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTHL
                     CDVEIPGQGPMRESNYTKPGGTLEPPVKTPAGKVGLAICYDMRFPELSLKLAQAGAEI
                     LTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASYGHSMVVDPWGT
                     VVARCSEGPGLCLARIDLHFLQQMRQHLPVFQHRRPDLYGSLGHPLS"
     exon            79..165
                     /gene="Nit1"
                     /gene_synonym="ESTM30"
                     /inference="alignment:Splign:2.1.0"
     exon            166..417
                     /gene="Nit1"
                     /gene_synonym="ESTM30"
                     /inference="alignment:Splign:2.1.0"
     exon            418..521
                     /gene="Nit1"
                     /gene_synonym="ESTM30"
                     /inference="alignment:Splign:2.1.0"
     exon            522..655
                     /gene="Nit1"
                     /gene_synonym="ESTM30"
                     /inference="alignment:Splign:2.1.0"
     exon            656..781
                     /gene="Nit1"
                     /gene_synonym="ESTM30"
                     /inference="alignment:Splign:2.1.0"
     exon            782..1342
                     /gene="Nit1"
                     /gene_synonym="ESTM30"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcccacttgc tcctgcgctc ctggctcctg gcagctcttg agatccgacc ccgtgaatgg
       61 ttttggctgt gtcttcatgc tgggcttcat caccaggcct cctcaccaac tcctgtgtac
      121 cggataccga ttacttcgaa ccccagtact ttgtactcag cccaggccca gaaccatgtc
      181 ctcatcaact tcctgggagc tgcccctggt ggctgtgtgc caggtaacat caacaccaaa
      241 caagcaagag aactttaaaa catgtgctga gttggttcaa gaggctgcca gactgggtgc
      301 ttgcctggcc tttctgcctg aggcatttga ctttattgca cgaaaccctg ccgagacatt
      361 actcctgtcc gaaccactga atggggatct tttgggccaa tatagccagc ttgccaggga
      421 atgtggaatc tggctgtcct tgggcggttt ccacgagcgt ggccaagact gggagcagaa
      481 tcagaaaatc tacaattgtc atgtgctttt gaacagcaag ggatcagtag tggccagtta
      541 caggaagaca catctgtgcg atgtagagat cccaggtcag gggccgatga gagaaagcaa
      601 ctataccaag cctggaggca ctcttgagcc acctgtcaag acaccggctg gcaaggttgg
      661 tctagcaatc tgttatgaca tgcggttccc tgaactttct ttgaaattgg ctcaagctgg
      721 ggcagaaata cttacttatc cttcagcctt tggatctgtt acaggtccag cccactggga
      781 ggtgctgctg cgggcccgcg ccattgaatc tcagtgctat gtaatagcag cagcgcagtg
      841 tggacgccac catgaaacaa gagcaagtta tggccatagc atggtggttg acccgtgggg
      901 cacagtggtg gcccgctgct ccgagggacc aggcctctgc cttgctcgaa ttgatctcca
      961 ctttctacaa cagatgcgcc aacacctgcc tgtgttccag caccgcagac ctgacctcta
     1021 tggcagtctg ggtcatccac tctcttaatg cttttaactt ctgttggtta agacacagac
     1081 acagccaact gggaggtgat gctactgtga cttgtaggca ggacacaagc acggttccct
     1141 tcacttgcag aactttaacc tctcttaatg gaacacaggc ttaatcaagt ccatgggaaa
     1201 gaagagactt tgacctgacc tcagatttca gtttcagaaa agtgggattt tatatagtca
     1261 ctgtttattt catgaaaact gaagttatgc tgagggctga gcagcactgg cattgaaaaa
     1321 aaaatataat aatcataatg tc
//
