LOCUS       NM_022656               5606 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus nischarin (Nisch), transcript variant 1, mRNA.
ACCESSION   NM_022656
VERSION     NM_022656.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Zheng,P., Pan,C., Zhou,C., Liu,B., Wang,L., Duan,S. and Ding,Y.
  TITLE     Contribution of Nischarin/IRAS in CNS development, injury and
            diseases
  JOURNAL   J Adv Res 54, 43-57 (2023)
   PUBMED   36716956
  REMARK    GeneRIF: Contribution of Nischarin/IRAS in CNS development, injury
            and diseases.
            Review article
REFERENCE   3  (bases 1 to 5606)
  AUTHORS   Yang,Z.H., Cai,X., Ding,Z.L., Li,W., Zhang,C.Y., Huo,J.H.,
            Zhang,Y., Wang,L., Zhang,L.M., Li,S.W., Li,M., Zhang,C., Chang,H.
            and Xiao,X.
  TITLE     Identification of a psychiatric risk gene NISCH at 3p21.1 GWAS
            locus mediating dendritic spine morphogenesis and cognitive
            function
  JOURNAL   BMC Med 21 (1), 254 (2023)
   PUBMED   37443018
  REMARK    GeneRIF: Identification of a psychiatric risk gene NISCH at 3p21.1
            GWAS locus mediating dendritic spine morphogenesis and cognitive
            function.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5606)
  AUTHORS   Li,S., Zhang,X.Q., Liu,C.C., Wang,Z.Y., Lu,G.Y., Shen,H.W., Wu,N.,
            Li,J. and Li,F.
  TITLE     IRAS/Nischarin modulates morphine reward by glutamate receptor
            activation in the nucleus accumbens of mouse brain
  JOURNAL   Biomed Pharmacother 153, 113346 (2022)
   PUBMED   36076473
REFERENCE   5  (bases 1 to 5606)
  AUTHORS   Gupta,S., Bazargani,N., Drew,J., Howden,J.H., Modi,S., Al
            Awabdh,S., Marie,H., Attwell,D. and Kittler,J.T.
  TITLE     The non-adrenergic imidazoline-1 receptor protein nischarin is a
            key regulator of astrocyte glutamate uptake
  JOURNAL   iScience 25 (4), 104127 (2022)
   PUBMED   35434559
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5606)
  AUTHORS   Quwailid,M.M., Hugill,A., Dear,N., Vizor,L., Wells,S., Horner,E.,
            Fuller,S., Weedon,J., McMath,H., Woodman,P., Edwards,D.,
            Campbell,D., Rodger,S., Carey,J., Roberts,A., Glenister,P.,
            Lalanne,Z., Parkinson,N., Coghill,E.L., McKeone,R., Cox,S.,
            Willan,J., Greenfield,A., Keays,D., Brady,S., Spurr,N., Gray,I.,
            Hunter,J., Brown,S.D. and Cox,R.D.
  TITLE     A gene-driven ENU-based approach to generating an allelic series in
            any gene
  JOURNAL   Mamm Genome 15 (8), 585-591 (2004)
   PUBMED   15457338
REFERENCE   7  (bases 1 to 5606)
  AUTHORS   Alahari,S.K., Reddig,P.J. and Juliano,R.L.
  TITLE     The integrin-binding protein Nischarin regulates cell migration by
            inhibiting PAK
  JOURNAL   EMBO J 23 (14), 2777-2788 (2004)
   PUBMED   15229651
  REMARK    GeneRIF: Nisch regulates cell movement by inhibiting PAK.
REFERENCE   8  (bases 1 to 5606)
  AUTHORS   McClive,P.J., Hurley,T.M., Sarraj,M.A., van den Bergen,J.A. and
            Sinclair,A.H.
  TITLE     Subtractive hybridisation screen identifies sexually dimorphic gene
            expression in the embryonic mouse gonad
  JOURNAL   Genesis 37 (2), 84-90 (2003)
   PUBMED   14595844
REFERENCE   9  (bases 1 to 5606)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   10 (bases 1 to 5606)
  AUTHORS   Alahari,S.K., Lee,J.W. and Juliano,R.L.
  TITLE     Nischarin, a novel protein that interacts with the integrin alpha5
            subunit and inhibits cell migration
  JOURNAL   J Cell Biol 151 (6), 1141-1154 (2000)
   PUBMED   11121431
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ106892.1, AK122412.1, BC089329.1, AC154446.2 and BP761152.1.
            
            On Sep 8, 2006 this sequence version replaced NM_022656.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164131,
                              SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                CJ106892.1         2-26
            26-1157             AK122412.1         1-1132
            1158-2192           BC089329.1         377-1411
            2193-2193           AC154446.2         142186-142186       c
            2194-5584           BC089329.1         1413-4803
            5585-5606           BP761152.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 19.09 cM"
     gene            1..5606
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /note="nischarin"
                     /db_xref="GeneID:64652"
                     /db_xref="MGI:MGI:1928323"
     exon            1..260
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..4946
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     imidazoline receptor I-1-like protein; I1R; IR1;
                     imidazoline receptor 1; imidazoline-1 receptor"
                     /codon_start=1
                     /product="nischarin isoform 1"
                     /protein_id="NP_073147.2"
                     /db_xref="CCDS:CCDS36853.1"
                     /db_xref="GeneID:64652"
                     /db_xref="MGI:MGI:1928323"
                     /translation="MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHE
                     WTIKHRYSDFHDLHEKLVAERKIDKSLLPPKKIIGKNSRSLVEKRERDLEVYLQTLLT
                     TFPDVAPRVLAHFLHFHLYEVNGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITE
                     QLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIKEQLLPFDLSIFK
                     SLHQVEISHCDAKHIRGLVTSKPTLATMSVRFSATSMKEVLAPEASEFDEWEPEGTAT
                     LGGPVTAIIPTWQALTTLDLSHNSICEIDESVKLIPKIEYLDLSHNGLRVVDNLQHLY
                     NLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLESLSGLHKLYSLVNVDLRDNRIE
                     QLDEVKSIGSLPCLERLTLLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELD
                     TVEVLKAIQKAKDVKSKLSNTEKKAGEDFRLPPAPCIRPGGSPPAAPASASLPQPILS
                     NQGIMFVQEEALASSLSSTDSLPPEDHRPIARACSDSLESIPAGQVASDDLRDVPGAV
                     GGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWI
                     EAANQREEAHGEQGEEEEEEEEEEDVAENRYFEMGPPDAEEEEGSGQGEEDEEDEDEE
                     AEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFE
                     IPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAA
                     NLHEFHADLRSCFAPQHMAMLCSPILYGSHTTLQEFLRQLLTFYKVAGGSQERSQGCF
                     PVYLVYSDKRMVQTPAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTS
                     QHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVL
                     ELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVISKNPSAKPRNQPAKSRA
                     SAEQRLQETPADAPAPAAVPPTASAPAPAEALAPDLAPVQAPGEDRGLTSAEAPAAAE
                     APAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAPAEAPAAAEAPAAAEAPAAAEAPAA
                     AEAPASAEAPAPNQAPAPARGPAPARGPAPAGGPAPAEALAQAEVPAQYPSERLIQST
                     SEENQIPSHLPVCPSLQHIARLRGRAIIDLFHNSIAEVENEELRHLLWSSVVFYQTPG
                     LEVTACVLLSSKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHVSQCFVLKLSD
                     LQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPVD
                     KDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYIVAQKMADPAKNPALSI
                     LLYIQAFQVVTPHLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRY
                     RLDDGRRVRDLDRVLMGYYPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRVGQ
                     GREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG"
     exon            261..344
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            345..527
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            528..576
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            577..740
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            741..836
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            837..932
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1088
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1157
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1343
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1472
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1586
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1695
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1823
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1873
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..3256
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3257..3887
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3888..4044
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4045..4173
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4174..4335
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4336..5586
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5563..5568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /note="hexamer: AATAAA"
     polyA_site      5584
                     /gene="Nisch"
                     /gene_synonym="1200007D05Rik; 3202002H23Rik; edsn; I-1"
                     /note="major polyA site"
ORIGIN      
        1 attacgggca aggggcgggg cgaggacgca agcaggccga ggggccaaga tggcgtcgcg
       61 ggcgcgggcg gggggcagcg ggggctgggg ggcggtggga cgctgacccc gccccctgct
      121 gctgccactc tgcgccgggc gtcggcagcg gtggagccgg caacatggcg gctgcgacac
      181 tcagcttcgg ccctgagcgg gaggccgagc ctgccaagga ggcgcgcgtc gtgggttctg
      241 agctcgtgga cacgtacacg gtttatgtca tccaggttac cgatggcaac catgagtgga
      301 cgatcaaaca ccgttacagt gattttcatg acctacatga aaagcttgtg gcagagagaa
      361 aaattgacaa atctctactt ccacccaaaa agataattgg gaagaactcg agaagcttgg
      421 tagagaagag ggagagagac ctggaggtgt acctccagac gctcctcaca accttccctg
      481 atgtggctcc cagagttctg gcccacttcc tgcattttca cctctatgaa gtaaatggtg
      541 tcactgcagc actggctgaa gaactctttg aaaaaggaga gcaacttcta ggagccggtg
      601 aggtctttgc catcaggccc ttgcagctct atgctatcac agaacagctg caacagggaa
      661 agcctacttg tgccagtggt gatgccaaga ctgaccttgg acacatccta gacttcacct
      721 gtcgccttaa gtatcttaag gtttcaggca cagaaggacc ttttgggacc agcaatatta
      781 aggagcagct cttgcccttt gatctttcaa tattcaagtc tcttcaccag gtggagataa
      841 gtcattgtga tgccaagcat atccgagggc tggtcacctc caagcccact ttagccacaa
      901 tgagtgttag attctcagca acctcaatga aggaagtgct tgctcctgaa gcctcagaat
      961 ttgatgagtg ggagcctgaa ggcactgcta ccctcggagg ccctgtgact gctatcatcc
     1021 caacatggca agcactgact actctagacc tgagccacaa cagcatctgt gagatcgacg
     1081 aatctgtgaa actgatccca aagatagagt acctggacct gagtcacaat ggactgcgag
     1141 ttgtggataa cctgcagcac ctatacaacc tcgtgcacct tgacctgtcc tacaacaagc
     1201 tctcctccct ggaaggcgtg cacactaaac tgggcaatgt caaaacccta aacctggcag
     1261 gcaacttctt agagagtctg agcggtctgc acaaactcta ctccttggtt aatgtggacc
     1321 tacgagacaa ccggattgag cagttggatg aggtcaagag cattggcagc ctgccatgtc
     1381 tggagcgttt gaccttgctg aacaaccctt tgagcatcat ccctgactac cggaccaagg
     1441 tgctttccca gtttggagaa cgagcctctg agatttgtct agatgatgtc gcaaccacag
     1501 agaaagagct ggacactgtg gaagtgctga aggcaattca gaaagccaaa gatgtcaagt
     1561 ccaaactgag caacacagaa aagaaggctg gtgaggactt ccggctcccg cctgcaccct
     1621 gcatcagacc cggcggctcc cctcctgcag ctcccgcctc agcctccctg cctcagccga
     1681 tcctctccaa ccaaggtatc atgtttgtac aggaagaggc cctggccagc agcctctcat
     1741 ccacggatag tctgccaccc gaggaccacc ggcccattgc ccgagcctgc tctgactcct
     1801 tggaatctat ccctgcagga caggtggcct ctgatgatct gagggatgtg ccaggagctg
     1861 ttggcggcgt gagcccagat catgcagagc cagaggttca ggtggtgcct gggtccggcc
     1921 agatcatctt cctgcccttc acttgcattg gctacacagc caccaaccag gactttatcc
     1981 agcgcctcag cacactgatc cgccaggcca ttgaacggca actccctgcc tggattgagg
     2041 ctgccaacca gcgcgaggaa gcccatggtg agcagggcga ggaggaagaa gaggaagagg
     2101 aagaggagga tgttgctgag aaccgctact ttgaaatggg acccccagac gcagaggaag
     2161 aggaggggag tggccaggga gaagaggatg aggaagacga ggacgaggag gcggaggagg
     2221 agcgcctggc tctggagtgg gccctgggcg ccgatgagga cttcctgctg gagcacatcc
     2281 gcatcctcaa ggtgctctgg tgcttcctga tccacgtgca aggcagcatc cgccagttcg
     2341 ctgcctgcct tgtgctcact gacttcggca tcgcagtctt tgagatccca caccaagagt
     2401 caagaggcag cagccagcac atcctctcat ccctgcgttt tgtcttctgc ttcccacatg
     2461 gcgacctcac ggagtttggc ttcctcatgc ccgagctgtg tctggtgctc aaggtgcggc
     2521 acagtgagaa cacgctcttc atcatctcgg atgctgccaa cttacacgag ttccatgctg
     2581 atctacgctc ctgctttgca ccgcagcaca tggccatgct gtgcagcccc atcctctacg
     2641 gcagccacac caccctgcag gagttcttgc gccagctgct caccttctac aaggtggccg
     2701 ggggctctca ggagcgcagc cagggctgct tccctgtcta cctggtctac agtgacaagc
     2761 gcatggtcca gacccctgcc ggggactatt caggcaatat cgaatgggcc agctgcacgc
     2821 tgtgctcggc ggtgcggcgc tcctgctgcg cgccctcgga ggccgtcaag tccgctgcca
     2881 tcccctactg gctgctgctc acatcccagc atctcaacgt catcaaggcc gacttcaacc
     2941 ccatgcccaa tcgaggcacc cacaactgcc gcaaccgcaa cagcttcaag cttagccgcg
     3001 tcccgctctc taccgtgctg ctagacccca ctcgcagctg cacccagcca cggggtgcct
     3061 tcgctgatgg ccatgtgctc gagctgctcg tgggctaccg cttcgttacc gccatctttg
     3121 tgctgcccca cgagaaattc cacttcctgc gagtctacaa tcagctacgc gcctcactgc
     3181 aggacctgaa gactgtggtc atctccaaga atccttcagc taagccaaga aatcagcctg
     3241 ccaagagcag ggccagtgct gagcagcggc tacaggagac cccagcagac gctccggctc
     3301 cagctgcagt cccaccaaca gcttcagctc cagcccccgc agaggccttg gctccagatc
     3361 tggctcctgt acaggcccca ggagaggacc gaggtctaac ttcagcagag gctccagccg
     3421 cagcagaggc tccagccgca gcagaggctc cagccgcagc agaggctcca gccgcagcag
     3481 aggctccagc cgcagcagag gctccagccg cagcagaggc tccggcccca gcagaggctc
     3541 cagccgcagc agaggctccg gccgcagcag aggctccagc tgcagcagag gctccagccg
     3601 cagcagaggc tccagcttca gcagaggctc cagctccaaa ccaggctcct gctccagcaa
     3661 ggggtcccgc tccagcaagg ggtcccgctc cagcaggggg tcccgctcca gcagaggccc
     3721 tggcacaagc agaagtccct gcccagtacc caagtgagcg cctaatccag tccacatctg
     3781 aagagaatca gatcccttct cacttgccag tatgcccatc actccagcac attgcccgtc
     3841 ttcgggggag agccatcatt gacctcttcc acaacagcat tgctgaggtt gaaaatgagg
     3901 agctgaggca cctcctgtgg tcatcagtgg tgttctacca gaccccgggg ctagaagtga
     3961 ccgcctgtgt gctgctctct agcaaggctg tgtacttcat actgcatgat ggtctccgcc
     4021 gctacttctc tgaaccactg caggatttct ggcaccagaa aaacaccgac tataacaaca
     4081 gtcctttcca cgtctctcag tgctttgtgt tgaaactcag tgacctgcag tcagtcaacg
     4141 tcggcctttt cgaccagtac ttccggctga cgggctcctc cccgacgcag gtggtcacgt
     4201 gcttgactcg cgacagctac ctgacgcact gcttcctcca gcatctgatg cttgtgctgt
     4261 cctccctgga gcgcacaccc tcgcctgagc ctgttgacaa ggacttctac tcagaatttg
     4321 gggacaagaa tacagggaaa atggagaact atgagctgat ccattccagc cgcgtcaagt
     4381 tcacctaccc cagtgaggaa gaggttgggg acctgaccta tattgtcgca cagaagatgg
     4441 ctgatcctgc aaagaatcca gccctcagca tcttactgta catccaggcc ttccaggtgg
     4501 tcacaccaca ccttgggcgg ggcaggggcc cactgcgccc taagacgctg ctcctgacca
     4561 gcgccgagat cttcctcctg gatgaggact acatccacta tccattgcct gaatttgcca
     4621 aagagccacc gcagagggac agataccggc tagacgatgg ccgccgggtc cgggatttgg
     4681 accgggtgct catgggctac tatccctacc cacaggccct cactcttgtt tttgatgaca
     4741 cacagggtca cgacctcatg gggagtgtca ccctggacca ctttggggag atgccaggtg
     4801 gtcctggcag ggttggccag ggccgagagg tacagtggca ggtgtttgtc cccagtgccg
     4861 agagccgaga aaagctcatc tcactgctcg cacgacagtg ggaagctctt tgtggcaggg
     4921 agctgcctgt ggagctcact ggctagtgca cgccatagca gccctggcct cctgccatgc
     4981 ccagcctagc ctggtgcccg tgaaggcagg gaagcggcct tctgcgttct ttttaaggtc
     5041 tttctcctct cccttggttc ctacattgat tgcccgtggc ccccacacat gtgaccagct
     5101 cccacagaga ctgtgaacat gtgtgatcag ttaatcctaa cgctgggaag acatatgcca
     5161 gccccaccag agcgtccttg tgctgttagc gtcccttgtg tcgcctgcct ctgaagtggc
     5221 atggtcagac gcgccagcat tgttttgtct gtgattgatg ctgttgttag cgtggcgctg
     5281 tggacctgat aacccccacg tccttttctg aagtgttaag tccagtcctt cgttgctgtt
     5341 tgctgtcatt gtgggcgtgc cgctgctaat tacaaagctg gtagcagagt acacaggcaa
     5401 gctcccacgt tgtgggcttt tccaaaatgg aggcctcctc aagtccagag cagaggaggc
     5461 cctatgggga gttcccaggt catgggtcca agaggagcca gtacagggga cctagagctg
     5521 ccagcaccaa gtataattcc tgttgcctac gttctctatt ctaataaaat ggagtttgac
     5581 acaacaaaaa aaaaaaaaaa aaaaaa
//
