LOCUS       NM_027707              10216 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus NIPBL cohesin loading factor (Nipbl), transcript
            variant a, mRNA.
ACCESSION   NM_027707 NM_198293
VERSION     NM_027707.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Chea,S., Kreger,J., Lopez-Burks,M.E., MacLean,A.L., Lander,A.D. and
            Calof,A.L.
  TITLE     Gastrulation-stage gene expression in Nipbl+/- mouse embryos
            foreshadows the development of syndromic birth defects
  JOURNAL   Sci Adv 10 (12), eadl4239 (2024)
   PUBMED   38507484
  REMARK    GeneRIF: Gastrulation-stage gene expression in Nipbl[+/-] mouse
            embryos foreshadows the development of syndromic birth defects.
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   Boulet,F., Odelin,G., Harrington,A. and Moore-Morris,T.
  TITLE     Nipbl Haploinsufficiency Leads to Delayed Outflow Tract Septation
            and Aortic Valve Thickening
  JOURNAL   Int J Mol Sci 24 (21), 15564 (2023)
   PUBMED   37958548
  REMARK    GeneRIF: Nipbl Haploinsufficiency Leads to Delayed Outflow Tract
            Septation and Aortic Valve Thickening.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10216)
  AUTHORS   Kean,C.M., Tracy,C.J., Mitra,A., Rahat,B., Van Winkle,M.T.,
            Gebert,C.M., Noeker,J.A., Calof,A.L., Lander,A.D., Kassis,J.A. and
            Pfeifer,K.
  TITLE     Decreasing Wapl dosage partially corrects embryonic growth and
            brain transcriptome phenotypes in Nipbl+/- embryos
  JOURNAL   Sci Adv 8 (48), eadd4136 (2022)
   PUBMED   36449618
REFERENCE   4  (bases 1 to 10216)
  AUTHORS   Linares-Saldana,R., Kim,W., Bolar,N.A., Zhang,H.,
            Koch-Bojalad,B.A., Yoon,S., Shah,P.P., Karnay,A., Park,D.S.,
            Luppino,J.M., Nguyen,S.C., Padmanabhan,A., Smith,C.L., Poleshko,A.,
            Wang,Q., Li,L., Srivastava,D., Vahedi,G., Eom,G.H., Blobel,G.A.,
            Joyce,E.F. and Jain,R.
  TITLE     BRD4 orchestrates genome folding to promote neural crest
            differentiation
  JOURNAL   Nat Genet 53 (10), 1480-1492 (2021)
   PUBMED   34611363
  REMARK    Erratum:[Nat Genet. 2021 Dec;53(12):1723. doi:
            10.1038/s41588-021-00980-2.. PMID: 34725478]
REFERENCE   5  (bases 1 to 10216)
  AUTHORS   Weiss,F.D., Calderon,L., Wang,Y.F., Georgieva,R., Guo,Y.,
            Cvetesic,N., Kaur,M., Dharmalingam,G., Krantz,I.D., Lenhard,B.,
            Fisher,A.G. and Merkenschlager,M.
  TITLE     Neuronal genes deregulated in Cornelia de Lange Syndrome respond to
            removal and re-expression of cohesin
  JOURNAL   Nat Commun 12 (1), 2919 (2021)
   PUBMED   34006846
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10216)
  AUTHORS   Kawauchi,S., Calof,A.L., Santos,R., Lopez-Burks,M.E., Young,C.M.,
            Hoang,M.P., Chua,A., Lao,T., Lechner,M.S., Daniel,J.A.,
            Nussenzweig,A., Kitzes,L., Yokomori,K., Hallgrimsson,B. and
            Lander,A.D.
  TITLE     Multiple organ system defects and transcriptional dysregulation in
            the Nipbl(+/-) mouse, a model of Cornelia de Lange Syndrome
  JOURNAL   PLoS Genet 5 (9), e1000650 (2009)
   PUBMED   19763162
  REMARK    GeneRIF: Mice heterozygous for a gene-trap mutation in Nipbl were
            produced and exhibited defects characteristic of Cornelia de Lange
            Syndrome.
REFERENCE   7  (bases 1 to 10216)
  AUTHORS   Mattar,P., Britz,O., Johannes,C., Nieto,M., Ma,L., Rebeyka,A.,
            Klenin,N., Polleux,F., Guillemot,F. and Schuurmans,C.
  TITLE     A screen for downstream effectors of Neurogenin2 in the embryonic
            neocortex
  JOURNAL   Dev Biol 273 (2), 373-389 (2004)
   PUBMED   15328020
REFERENCE   8  (bases 1 to 10216)
  AUTHORS   Krantz,I.D., McCallum,J., DeScipio,C., Kaur,M., Gillis,L.A.,
            Yaeger,D., Jukofsky,L., Wasserman,N., Bottani,A., Morris,C.A.,
            Nowaczyk,M.J., Toriello,H., Bamshad,M.J., Carey,J.C., Rappaport,E.,
            Kawauchi,S., Lander,A.D., Calof,A.L., Li,H.H., Devoto,M. and
            Jackson,L.G.
  TITLE     Cornelia de Lange syndrome is caused by mutations in NIPBL, the
            human homolog of Drosophila melanogaster Nipped-B
  JOURNAL   Nat Genet 36 (6), 631-635 (2004)
   PUBMED   15146186
REFERENCE   9  (bases 1 to 10216)
  AUTHORS   Tonkin,E.T., Wang,T.J., Lisgo,S., Bamshad,M.J. and Strachan,T.
  TITLE     NIPBL, encoding a homolog of fungal Scc2-type sister chromatid
            cohesion proteins and fly Nipped-B, is mutated in Cornelia de Lange
            syndrome
  JOURNAL   Nat Genet 36 (6), 636-641 (2004)
   PUBMED   15146185
REFERENCE   10 (bases 1 to 10216)
  AUTHORS   Thuret,S., Bhatt,L., O'Leary,D.D. and Simon,H.H.
  TITLE     Identification and developmental analysis of genes expressed by
            dopaminergic neurons of the substantia nigra pars compacta
  JOURNAL   Mol Cell Neurosci 25 (3), 394-405 (2004)
   PUBMED   15033168
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AJ627033.1, BC055787.1, AK078435.1, AK135347.1 and AV246194.1.
            
            On Nov 2, 2013 this sequence version replaced NM_027707.2.
            
            Transcript Variant: This variant (a) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ627033.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8815              AJ627033.1         1-8815
            8816-9074           BC055787.1         668-926
            9075-10084          AK078435.1         391-1400
            10085-10210         AK135347.1         2187-2312
            10211-10216         AV246194.1         241-246
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 3.82 cM"
     gene            1..10216
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /note="NIPBL cohesin loading factor"
                     /db_xref="GeneID:71175"
                     /db_xref="MGI:MGI:1913976"
     exon            1..339
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            340..482
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     CDS             419..8815
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /note="isoform a is encoded by transcript variant a;
                     nipped-B-like protein; SCC2 homolog; delangin homolog;
                     Nipped-B homolog"
                     /codon_start=1
                     /product="nipped-B-like protein isoform a"
                     /protein_id="NP_081983.2"
                     /db_xref="CCDS:CCDS37035.1"
                     /db_xref="GeneID:71175"
                     /db_xref="MGI:MGI:1913976"
                     /translation="MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNS
                     RIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLAR
                     SPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQT
                     SSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNI
                     HDNKVSGPLSGNSANHHADNPRHGSSDDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQ
                     PVCSPAGSDGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKIKLGKD
                     EKDQNEKAAMYDIISSPTKDSTKLTLRLSRVRSSDMDQQDDMLSGMENSNVSENDIPF
                     NVQYPGQTSKTPITPQDVNRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVATKQPQT
                     SVVQNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQD
                     SYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKK
                     PEETKQCNDAPISVLQEDIVGSLKSIPENHPETPKKKSDPELSKSEMKQNESRLSESK
                     PNENQLGESKSNESKLETKTETPTEELKQNENKTTESKQSESAVVEPKQNENRPCDTK
                     PNDNKQNNTRSENTKARPETPKQKAESRPETPKQKSEGRPETPKQKGDGRPETPKQKS
                     EGRPETPKQKGEGRPETPKHRHENRRDSGKPSTEKKPDVSKHKQDIKSDSPRLKSERA
                     EALKQRPDGRWESLRRDHDSKQKSDDRGESERHRGDQSRVRRPETLRSSSRNDHSTKS
                     DGSKTEKLERKHRHESGDSRDRPSGEQKSRPDSPRVKQGDTNKSRPGFKSPNSKDDKR
                     TEGNRSKVDSNKAHTDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMDM
                     KGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREEKSRSSLKSIKNKPS
                     KSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDN
                     DSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHDGRRSSGGGRYRNRSPS
                     DSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENIL
                     DNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNI
                     LEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKA
                     VYIEDVIERVIQYTKFHLQNTLYPQYDPVYRVDPHGGGLLSSKAKRAKCSTHKQRVIV
                     MLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVF
                     SRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDVDGEPMYIQMVTALVLQLIQC
                     VVHLPSSEKDPNSEEDSNKKVDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDY
                     RPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTV
                     AARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRK
                     FYIAQWFRDTTLETEKAMKSQKDEESSDATHHAKELETTGQIMHRAENRKKFLRSIIK
                     TTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRT
                     KAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQ
                     YYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLV
                     NETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSY
                     KPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVK
                     HAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKY
                     GMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKP
                     ALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGL
                     GFAFIQHPSLMFEQEVKNLYNSILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRD
                     WKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQG
                     LIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQA
                     INTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTEVTML
                     LYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKTSPAK
                     ENESSESEEEVSRPRKSRKRVDSESDSDSEDDINSVMKCLPENSAPLIEFANVSQGIL
                     LLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMA
                     NSKLTEDVKRSIVRQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPK
                     NNTAADTEDEESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKY
                     KDRPQIARVVQRTSSGVSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKI
                     ALTSANKLTNKVVQTLRSLYAAKDGTSS"
     exon            483..648
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            649..776
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            777..876
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            877..1028
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1189
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1286
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1913
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..3521
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            3522..3704
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            3705..3902
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            3903..3974
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            3975..4064
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            4065..4168
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            4169..4255
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            4256..4487
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            4488..4639
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            4640..4720
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            4721..4821
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            4822..4960
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            4961..5043
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            5044..5176
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            5177..5320
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            5321..5410
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            5411..5625
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            5626..5728
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            5729..5827
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            5828..5974
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            5975..6109
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            6110..6208
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            6209..6262
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            6263..6371
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            6372..6508
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            6509..6649
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            6650..6743
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            6744..6898
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            6899..6989
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            6990..7163
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            7164..7354
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            7355..7462
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            7463..7663
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            7664..7810
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            7811..8085
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            8086..8260
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            8261..8449
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     exon            8450..10216
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9131..9136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /note="hexamer: AATAAA"
     polyA_site      9153
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /note="major polyA site"
     regulatory      9397..9402
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /note="hexamer: AATAAA"
     polyA_site      9423
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
     regulatory      9713..9718
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /note="hexamer: TATAAA"
     polyA_site      9734
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
     regulatory      10198..10203
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
                     /note="hexamer: ATTAAA"
     polyA_site      10216
                     /gene="Nipbl"
                     /gene_synonym="Idn3"
ORIGIN      
        1 ccgacaccag caaagccgcc agggagggac gcccccgccg ccaggaggat tggttcccgg
       61 gcccgcggcg atgccccccc ggtagctcgg ggcccccggc cgggtgtttg tgtgagtgtt
      121 tctatgtggg agaaggagga ggaggacgaa gaagaagccg cgatttgtct tctcggctgc
      181 tctccccccg gctctacatg ttccccgcac tgaggagacg gaagaggagc cgtagccacc
      241 ccccctcccg gcccggatta tagtctcgct ctctcgctct ctctctcttg ccaccgcggc
      301 ctcggcctcc gcttcggatt cagacgccga ttcgcccaga gtttgggaaa tggaaagtaa
      361 tgacagctgg cacctgaact aaatactttc atagacaaca ccattccaga acttcaggat
      421 gaatggggat atgccccatg tccccataac tacgcttgcg gggattgcta gtcttacgga
      481 cctcctgaac cagctgcctc tcccatctcc tttacctgct acaactacaa agagcctcct
      541 ctttaattca cgaatagcag aagaggtgaa ctgccttttg gcctgtaggg atgacaattt
      601 ggtttcacag cttgtccata gtctcaacca ggtatcaaca gatcacatag aactgaaaga
      661 taaccttggc agtgatgatc ccgaaggcga tatacccgtc ttgttgcagg ccgtcctggc
      721 aaggagtccg aacgttttca gggagaaaag catgcagaac agatatgtac agagtggaat
      781 gatgatgtct cagtataaac tttctcagaa ttccatgcac agtagtcctg catcttccaa
      841 ttaccaacaa accactatct cacatagccc ctccagccgg tttgtgccac cacagacaag
      901 ctctgggaac agatttatgc cacaacaaaa tagcccagtg cctagtccat atgccccaca
      961 gagtcctgca ggatacatgc catattccca tccttcaagt tacacaacac atccgcagat
     1021 gcagcaagca tcggtatcaa gtcctattgt tgccggtggc ttgagaaaca tccatgataa
     1081 caaagtttct ggtccattat ctggcaactc agctaaccat catgctgata accctagaca
     1141 tggctcaagt gacgactacc tacacatggt gcacaggcta agtagtgatg atggcgattc
     1201 ttcaacaatg aggaatgctg catctttccc cttaagatct cctcaaccag tgtgttctcc
     1261 tgctggaagt gacggaacac ctaaaggctc aagaccacct ttaattctac agtctcagtc
     1321 tttaccttgt tcatcacctc gagatgttcc tccagatatc ttactagatt ctccagaaag
     1381 aaaacaaaaa aagcaaaaga aaattaaatt aggcaaggat gaaaaagacc agaacgagaa
     1441 agctgcaatg tatgatataa ttagctctcc aaccaaggac tccactaaac ttacattaag
     1501 actttctcgt gtaagatctt cagatatgga ccagcaagac gatatgctat ctggtatgga
     1561 aaatagcaat gtttcagaaa atgatattcc ttttaatgtg cagtatccag gacagacttc
     1621 aaaaacaccc attactccac aggacgtaaa ccgcccactt aatgctgctc agtgtttgtc
     1681 gcagcaagaa caaacagcat tccttccagc aaatcaagtg cctgttttac aacagaacac
     1741 ttcagttgct acaaaacaac cacagacttc tgtagtacag aatcagcagc aggtatcaca
     1801 acaggggccg atatatgatg aagtcgaatt ggacgcgttg gcagaaattg aacggataga
     1861 gcgagaatca gccattgaaa gggagcgctt ctcaaaggaa gttcaagata aggataagcc
     1921 tttgaaaaaa agaaaacaag attcttaccc acaggaggct gggggtgcta caggaggtaa
     1981 tagaccagct tctcaggaga cgggttctac gggaaatggg tcaaggccag cattaatggt
     2041 tagcattgat cttcatcagg caggaagagt ggactctcag gcttctataa ctcaggattc
     2101 agactccata aaaaagcctg aagaaaccaa acagtgtaat gatgcgccca tttctgttct
     2161 tcaggaagat attgttggaa gtcttaagtc tataccagaa aaccatcctg agactcctaa
     2221 aaaaaagtct gatcctgagc tttcaaaaag cgaaatgaaa caaaatgaaa gtaggttgtc
     2281 agaatctaaa ccaaatgaaa atcagctggg ggagtcaaaa tcaaatgaaa gtaagttaga
     2341 aactaaaact gagaccccaa cagaagaact taagcagaac gagaacaaaa cgacagagtc
     2401 caaacagagc gagagcgctg tagttgagcc taaacagaat gaaaatagac cgtgtgacac
     2461 aaaaccaaat gacaacaaac agaacaacac caggtcagaa aacacaaaag caagacctga
     2521 aaccccaaag caaaaagctg aaagccggcc tgaaacccca aagcaaaaga gtgaaggccg
     2581 gcctgaaacc ccaaaacaga agggtgatgg acgacctgag actccaaagc aaaagagtga
     2641 aggccggcct gaaactccaa agcaaaaagg tgaaggccgg cctgaaactc caaaacatag
     2701 gcatgaaaat aggagggatt ctggaaagcc atcaacagag aagaaacccg atgtgtctaa
     2761 gcataaacag gatatcaaat ctgactcacc tcgattaaaa tcagaaagag ctgaagcctt
     2821 aaagcagaga cctgatgggc gatgggagtc actaagacgt gaccatgata gtaaacagaa
     2881 atcagatgac aggggtgagt cagagcgaca tcggggtgat cagtcaaggg ttcgaagacc
     2941 agaaacattg agatcttcta gtagaaatga ccatagcact aaatcagatg gttcaaaaac
     3001 tgaaaagcta gaaagaaaac acaggcatga atcaggggac tcacgggaca gaccttctgg
     3061 ggaacaaaaa tcaagaccag acagtcctcg tgtaaaacaa ggagatacta ataaatcaag
     3121 acctggtttt aagtcaccaa atagtaaaga tgacaaaagg acagagggta acagaagtaa
     3181 agtagacagt aataaagcgc acactgataa taaggccgaa tttccaagct atttgttggg
     3241 ggggaggtct ggtgcattga aaaatttcgt cattccaaaa atcaagaggg ataaagatgg
     3301 caacataact caggaaacta agaaaatgga catgaaagga gagcagaaag acaaagtaga
     3361 aaaaatggga ttagttgaag atctaaacaa aggagctaag cctgtagttg ttctgcaaaa
     3421 actatcgctg gatgatgtcc agaagctaat taaagataga gaggagaaat caagaagttc
     3481 ccttaagtct atcaagaata agccatcaaa gtcaaataaa ggtagtatag atcaatcagt
     3541 gttaaaagaa ttgccccctg agctcctggc agaaattgaa tctaccatgc cactttgtga
     3601 acgtgtgaaa atgaacaaac gcaagcgtag cacagttaat gaaaagccaa aatatgctga
     3661 aatcagttca gatgaagata atgatagtga tgaagcgttt gaatcctcta ggaaacggca
     3721 taaaaaagat gacgataaag cttgggaata cgaagagcgc gacagaagaa gctctgggga
     3781 tcataggaga agtggccact cccatgacgg aaggaggagc tcgggtggtg gtcgttaccg
     3841 aaaccgaagt ccgtccgatt ctgacatgga agattactcc cctcctccca gccttagtga
     3901 ggttgctaga aaaatgaaga aaaaagaaaa acaaaagaaa aggaaagctt acgagccaaa
     3961 gctaacgccc gaagaaatga tggattcttc aacttttaag agatttacag cctcaataga
     4021 gaatattttg gataatttag aagatatgga ttttactgca tttggtgatg acgatgagat
     4081 tcctcaggaa ctgcttttag gaaaacatca gcttaatgaa cttgggagtg aatctgctaa
     4141 aattaaagcc atgggtataa tggacaagct ttcaactgac aaaactgtga aagtcctaaa
     4201 tatattggag aagaatattc aggatggatc aaagctttcc actttgttaa atcataataa
     4261 tgatactgaa gaagaagaaa gattgtggag agacctaatt atggaaagag ttacaaagtc
     4321 agcagatgcc tgtcttacaa ctatcaacat tatgacatct cctaacatgc caaaagctgt
     4381 atacattgaa gatgtaattg aaagggttat acagtacact aagtttcatt tacagaacac
     4441 actctatccc cagtatgacc ctgtttacag agtagatcca catggaggag ggttattaag
     4501 ttcaaaggcc aaacgggcta aatgttctac tcataagcag agagtaattg taatgcttta
     4561 caacaaagtt tgtgacattg ttagcagttt atcagaattg ctagagatac agcttcttac
     4621 agacacaaca attcttcagg tttcatcaat gggaataaca cccttttttg tggaaaatgt
     4681 cagtgaacta caattgtgtg ccattaagtt agtcactgca gtattttcaa gatatgaaaa
     4741 gcataggcag ttaatattgg aagaaatttt tacttcactt gcaagattac caactagcaa
     4801 gagaagttta aggaatttca ggttaaatag tagtgatgtg gatggagaac ctatgtatat
     4861 tcagatggtt acagctctag ttttacaact tattcagtgc gtagttcact taccatcatc
     4921 ggagaaggat cctaattcag aagaagactc aaacaaaaaa gttgatcagg atgttgtcat
     4981 tacgaactct tacgaaacag ctatgagaac agcccaaaac tttctctcca tctttcttaa
     5041 aaaatgtggt agtaaacaag gagaagaaga ttacagaccg ctttttgaaa actttgttca
     5101 agaccttctc tcaacagtca ataagcccga gtggccggct gctgaactac tccttagctt
     5161 gttagggagg ctgttggttc atcagttcag taacaagtca acagaaatgg ctttaagagt
     5221 ggcatctctt gattaccttg ggactgttgc tgcgcgactg agaaaagatg ctgtcaccag
     5281 caagatggat caagggtcta tagaacgaat tttaaaacag gtttctggag gagaagatga
     5341 gatccaacaa ttgcaaaaag cattgcttga ttatctggat gagaacactg aaactgaccc
     5401 ttcgctagtg ttttctcgta aattctatat agcccagtgg ttccgtgaca caactctgga
     5461 aacagaaaaa gcaatgaaat cacaaaaaga tgaagaatca tctgacgcaa cacatcatgc
     5521 aaaagaactt gagacaactg ggcagatcat gcatcgcgct gagaaccgga aaaagtttct
     5581 tcgaagcatt atcaaaacca caccttctca gttcagcaca ttaaagatga actctgacac
     5641 tgtggactat gatgatgcgt gcttgattgt tcgatatttg gcctctatga ggccgtttgc
     5701 ccagagcttt gatatttatt tgacacagat actacgagtt cttggtgaaa atgcaattgc
     5761 cgttcgaaca aaagccatga agtgtttgtc tgaggttgtt gctgtagacc ccagtattct
     5821 agcaaggctt gacatgcagc gcggagttca tgggcgacta atggataact ccactagtgt
     5881 tcgagaggca gcagtggaat tgctaggtcg ctttgtcctg tgtcgccctc agcttgctga
     5941 gcagtattat gacatgctga ttgaaagaat attggatact gggatcagtg tcaggaaaag
     6001 agtaataaag attttaagag acatctgtat tgaacaacca acatttccaa aaattacaga
     6061 aatgtgtgta aaaatgattc gtagagtcaa tgatgaagag ggcattaaga aattagtaaa
     6121 tgaaacattc caaaagctct ggtttactcc aactccacat aatgacaaag aagcaatgac
     6181 aaggaagatt ttaaacatca ctgatgtggt tgcagcatgt agagatactg gatatgactg
     6241 gtttgagcaa ctccttcaaa atttgttgaa gtctgaagag gattcctcat ataaacctgt
     6301 gaagaaagct tgtactcaac ttgttgataa cctagttgaa catattctta aatatgagga
     6361 atctctagct gactcagaca ataaaggtgt aaattctgga agattggtag cttgtataac
     6421 cactttgttc ttattcagca aaataagacc ccaactcatg gttaaacatg ccatgactat
     6481 gcagccgtac ctcaccacta agtgtagtac gcaaaatgat ttcatggtta tctgcaatgt
     6541 tgcaaaaatc cttgagttag ttgtaccact gatggaacac ccaagtgaaa ctttccttgc
     6601 cactattgag gaagatctga tgaagctgat catcaaatac ggcatgactg tagtacaaca
     6661 ttgtgtgagc tgtcttgggg ctgttgtaaa taaagtgacc cagaatttta aatttgtgtg
     6721 ggcctgtttc aatagatact atggtgccat ttcaaaatta aaaagtcagc accaagaaga
     6781 cccaaataac acttcacttt taacaaataa accagcgctt cttaggtccc tcttcactgt
     6841 tggagcactt tgccgccatt ttgattttga tctggaagat tttaaaggca acagtaaggt
     6901 taacataaag gataaagtac ttgaactgtt gatgtatttt acaaaacact cagatgaaga
     6961 agtacaaaca aaagccatca ttggtctagg atttgccttt atacagcatc cgagtctaat
     7021 gtttgagcaa gaagtgaaga atctgtataa tagtatttta tctgataaga actcctcagt
     7081 gaatttaaaa atacaagtgt taaaaaacct ccagacttac ctacaagaag aagacacgcg
     7141 aatgcaacaa gcagacagag actggaagaa agttgcaaaa caggaagacc tgaaggaaat
     7201 gggggacgtc tcctcgggga tgagcagctc catcatgcag ctgtacctca agcaggtgct
     7261 ggaggcgttc tttcataccc agtcaagtgt gcgccacttc gccctgaatg tcattgctct
     7321 gactctgaac caaggcctca tccatccggt tcagtgtgtt ccatacttaa ttgccatggg
     7381 aacagatcca gaacctgcta tgcgaaacaa ggctgatcag cagcttgtgg agatagacaa
     7441 aaaatatgct gggttcatcc atatgaaagc cgtggctggt atgaagatgt cttaccaggt
     7501 ccaacaggcg atcaacacgt gcctaaaaga ccctgtaagg ggtttccgac aagatgaatc
     7561 ctccagtgct ctgtgctcac atctatactc catgatccgc gggaaccgcc aacacagacg
     7621 cgcctttctt atttctttac tcaacctctt tgatgacaca gcaaaaacag aagtgactat
     7681 gctcttgtac atagcagaca atctagcctg ttttccatac cagactcagg aagagccgct
     7741 gtttataatg caccacatag acattacgct ctctgtttcc ggtagtaacc tattgcagtc
     7801 attcaaggag tctatggtaa aagacaaaag gaaagagagg aaaacgtccc ctgctaaaga
     7861 gaacgagtcc agtgagagtg aagaagaagt gtccagacct cggaagtctc gaaagcgggt
     7921 agattcggag tcagactcag actctgaaga tgatataaat tcagtgatga agtgtttgcc
     7981 ggaaaattca gcccctttga ttgaattcgc aaatgtttcc cagggtattt tattacttct
     8041 tatgttaaag cagcacctga agaatctctg tggattttct gatagtaaaa ttcaaaaata
     8101 ttctccatct gaatctgcaa aagtgtatga taaagccatt aatcggaaaa caggggttca
     8161 ttttcatccc aaacaaacac tggacttcct gcgaagtgac atggctaatt ccaaactgac
     8221 ggaggatgtg aagaggagca tcgtaaggca gtacttagac tttaaacttc tcatggaaca
     8281 tctagaccct gatgaagaag aagaggaagg agaggtgtca gctagcacca atgctcggaa
     8341 caaagcaatt acctcgctgc ttggaggagg cagccctaaa aataacacag cagcagacac
     8401 agaagatgag gagagtgatg gggaggatag aggaggaggc acttcagggt cattgagaag
     8461 gtcaaaacga aattcagact ctacggagtt ggcagcacag atgaatgaaa gtgttgacgt
     8521 catggatgtc atcgctattt gctgtccaaa gtacaaagac cgaccacaga ttgcaagagt
     8581 agtacagaga accagcagtg gcgtcagtgt ccagtggatg gcaggctcct acagcggctc
     8641 ctggacggag gccaagcgcc gcgatggccg caaactggtg ccttgggtag acactattaa
     8701 agagtcagac attatttaca aaaaaattgc tctaacgagt gctaataagc tgactaataa
     8761 agttgttcag actttacgat ccctgtatgc cgccaaggat gggacttcca gctaatgaat
     8821 ttgtacatgc agccaaattt acaggaattt ttttaaaaag gcaaaaaaaa aaacttgaat
     8881 tatcaacatt ctggcaaaaa aaaatcagtt ttttgaagag tggaacctgg gatgcaggaa
     8941 caaaggaagg aatgttgggc agacattttt gtgggagctc ccttcgctgt tgtgcagcag
     9001 aaacgtctct cggttcgttt ttactcccac tgtattatag tttaacaaaa ttgtttatat
     9061 cttggaaaaa aaaactttct gtttaaaaaa taaacaagtg aatgttggaa attagtctgt
     9121 taatgttctt aataaagtgt tcttggagtt gaacctagca gcggatggct ttctttagct
     9181 tagcccagtt tccagggaag cgttgtttct ccaggctgta aaatggcaga ctctcctgga
     9241 tatataattt attctgttga gaaaaaaaaa agcatgcagt atctatgacc tatctgcaga
     9301 aggagttttt gtaaatgtag attttgttgt attaggtcac cctgaaaaca gtacaagaaa
     9361 aggggtcccc aggcactctg gtggagcgaa tactgcaata aattttttta cttctctttg
     9421 ttacttgtct gtttccattt gaatttctta ttgtaaagat ctgtttaaat ccatttatat
     9481 tattttgcag tcttttatgt aaaatttatt atatcattgg ttttcaaagc aaaacataaa
     9541 atattgttta tacagtttgt acaggctgac ttctgaataa ttggtatcta ttattttcat
     9601 tcccataaga gggtgtaaac aactccaggg ttttattgta tcctgcaata tttagtatta
     9661 actatatatg atttagcact gtgccaaaca cattttcaag agtacatttt gatataaaaa
     9721 gaaactatag tttattcctt gtatgcttcc atttctttct agtgatgtca tgatggtaat
     9781 acttattttt taaaccatgg ggaaagataa caattctgct attttggaaa aacagcattt
     9841 ctcttaaagt gacgccatgt tattttaaaa ctagagttga aataagtgtg acactttcta
     9901 gttaaacaca ctacattttg acacactatt tttaatgccg agagtgcttt tactagttgg
     9961 gatgttcatt ttgtagccta caagttttat atgcattttc agagcaattg tcatatagat
    10021 tagctacaaa tacctcagct aaattgggag gctattatat gtactgattt ttaaatcact
    10081 ttggaaaaaa aaatgacctc taaaatgtta aattttatga aatcagcaag taaatggcca
    10141 tcctcagtgt gtgaggacca gtggcgccca gcggcgacct cctctttgtg aacgcctatt
    10201 aaacacgatg aaaaca
//
