LOCUS       NM_172524               3295 bp    mRNA    linear   ROD 23-JUL-2024
DEFINITION  Mus musculus NIPA-like domain containing 4 (Nipal4), mRNA.
ACCESSION   NM_172524
VERSION     NM_172524.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Yamaji,M., Ohno,Y., Shimada,M. and Kihara,A.
  TITLE     Alteration of epidermal lipid composition as a result of deficiency
            in the magnesium transporter Nipal4
  JOURNAL   J Lipid Res 65 (6), 100550 (2024)
   PUBMED   38692573
  REMARK    GeneRIF: Alteration of epidermal lipid composition as a result of
            deficiency in the magnesium transporter Nipal4.
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Honda,Y., Kitamura,T., Naganuma,T., Abe,T., Ohno,Y., Sassa,T. and
            Kihara,A.
  TITLE     Decreased Skin Barrier Lipid Acylceramide and
            Differentiation-Dependent Gene Expression in Ichthyosis Gene
            Nipal4-Knockout Mice
  JOURNAL   J Invest Dermatol 138 (4), 741-749 (2018)
   PUBMED   29174370
  REMARK    GeneRIF: NIPAL4 is a putative Mg(2+) transporter, and Mg(2+)
            concentration in differentiated keratinocytes of Nipal4-KO mice was
            indeed lower than that of wild-type mice.
REFERENCE   3  (bases 1 to 3295)
  AUTHORS   Goytain,A., Hines,R.M. and Quamme,G.A.
  TITLE     Functional characterization of NIPA2, a selective Mg2+ transporter
  JOURNAL   Am J Physiol Cell Physiol 295 (4), C944-C953 (2008)
   PUBMED   18667602
REFERENCE   4  (bases 1 to 3295)
  AUTHORS   Lefevre,C., Bouadjar,B., Karaduman,A., Jobard,F., Saker,S.,
            Ozguc,M., Lathrop,M., Prud'homme,J.F. and Fischer,J.
  TITLE     Mutations in ichthyin a new gene on chromosome 5q33 in a new form
            of autosomal recessive congenital ichthyosis
  JOURNAL   Hum Mol Genet 13 (20), 2473-2482 (2004)
   PUBMED   15317751
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL713958.11 and AL663031.15.
            
            On May 1, 2007 this sequence version replaced NM_172524.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK035561.1, BC139819.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-126               AL713958.11        5574-5699           c
            127-366             AL663031.15        180215-180454       c
            367-423             AL663031.15        175127-175183       c
            424-514             AL663031.15        172965-173055       c
            515-675             AL663031.15        169622-169782       c
            676-3295            AL663031.15        166516-169135       c
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 27.59 cM"
     gene            1..3295
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /note="NIPA-like domain containing 4"
                     /db_xref="GeneID:214112"
                     /db_xref="MGI:MGI:2444671"
     exon            1..126
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..1304
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /note="ichthyin; NIPA-like protein 4; non-imprinted in
                     Prader-Willi/Angelman syndrome region protein 4 homolog"
                     /codon_start=1
                     /product="magnesium transporter NIPA4"
                     /protein_id="NP_766112.1"
                     /db_xref="CCDS:CCDS24572.1"
                     /db_xref="GeneID:214112"
                     /db_xref="MGI:MGI:2444671"
                     /translation="MELRVANANGSCENGSIVSLYCSSQEVLCQIVRGISPEEPYNAT
                     LITWQERVRKKYGFYIGVGLAFLSCFLIGTSVILKKKGLIRLVATGATRAVNGGYGYL
                     KDPMWWAGMATMSAGEVANFGAYAFAPATVVTPLGALSVLISAIFSSYCLGESLNLLG
                     KLGCVICMAGSTVMVIHAPKEEKVTTVAEMASKMKDTGFIVFAVLLVVSCLILIFIVA
                     PRYGQRNILIYIIICSVIGSFSVTAVKGLGVTIRNFFQGLPVVRHPLPYILSLILGLS
                     IIIQVNFLNRALDIFNTSLVFPIYYVFFTTVVVASSIVLFKEWYTMSAVDIVGTLSGF
                     VTIILGVFMLHAFKDLDINQISLPHTHKNPTPAPAPEPTVIKLEDKNVLVDNIELAST
                     PSPQQKPKVFMTDS"
     exon            127..366
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            367..423
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            424..514
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            515..675
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            676..3295
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2927..2932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2955
                     /gene="Nipal4"
                     /gene_synonym="9530066K23Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtgcctgccg gagccacgtt gggacaagtc acggcaacct gcccaggaac cgcggacctc
       61 gggcgctgtc agcccaggtc cccatggagc tgcgggtcgc caacgccaat ggcagctgcg
      121 agaacggctc catagtcagc ctctactgct cctctcagga ggtcctctgt cagatcgtga
      181 gaggcatcag tcctgaagag ccctacaatg ccaccttgat cacctggcaa gagagggtca
      241 ggaagaagta tggcttctac atcggtgtgg gcctcgcctt cctctcctgc ttcctcatcg
      301 gcaccagtgt catccttaag aagaaaggcc tcattcggct tgtggccaca ggggccacaa
      361 gggcagtgaa tggaggctac ggctacctga aggacccaat gtggtgggct ggaatggcta
      421 ccatgtctgc tggagaagtt gccaacttcg gggcctatgc atttgcacct gcgacggttg
      481 tcacaccgct gggagcactg agcgtcctca taagtgccat cttttcctcc tactgcctgg
      541 gagagagcct gaatctactg gggaaactgg gctgtgtgat ctgtatggct ggcagcacag
      601 tgatggtcat acatgccccc aaagaggaga aggtcaccac tgttgcagag atggcttcca
      661 agatgaaaga cacagggttc atcgtctttg ctgtccttct ggtggtgtct tgcctcatcc
      721 tcatcttcat tgttgcccca cgttacgggc aaaggaatat cctcatttac atcatcatct
      781 gctccgtgat cggatccttc tccgtgactg ccgtcaaggg gcttggtgtc accattagga
      841 acttcttcca gggcttacca gttgtgcggc accctctccc ttacatcctg tccctcatcc
      901 tgggtctttc tatcatcatc caggtcaact tcctcaacag ggcactggat attttcaaca
      961 cctcattggt cttccccatc tactacgttt tcttcaccac ggtggtggtg gcctcctcca
     1021 tcgtcctctt caaggagtgg tataccatgt ctgctgtgga cattgtgggc accctgtctg
     1081 gcttcgtcac catcatcctg ggtgttttta tgcttcacgc tttcaaggat ttggacatca
     1141 accaaatcag cttgccccac acgcacaaaa acccaactcc agctcccgcc ccagagccca
     1201 cagtcattaa actagaagac aagaatgtcc ttgtagataa catagaactt gccagcacac
     1261 catcacccca acagaagccc aaagtattta tgaccgattc ttaaagcctg gaatgcatga
     1321 gtaagaggag gcagccaatg gtccagcctg acctctcaag ttcacaacta cctggttact
     1381 ttcagcacat ctgttgccaa gggctagttt ctttttttga gatattcatt tatacctcac
     1441 tgctgttcct agacgaagaa gaaaaatccc tacccagtaa ggtaggggca ggacttcctg
     1501 gagatacagc cttggtgact aaatccacaa gtctctgtta gaaccagcaa gtccccagag
     1561 cctctcccct ctgggtccct caacaccacc atgttgttgg tagaatttga cctgctgaat
     1621 gtagcccagc tcaagaactt ccctcagcta tcggtcatcg ggaactcctt cacaccaatt
     1681 agcctgtcag gctggacttc tcagtcctta gcccgggaat ggagcttgtg ggggagtgca
     1741 gagcaatctg catgcctcgt catgccaagg tgtgtagcac atactaagta gcctggtgtt
     1801 tcttgtagac agaagcaaga ccccagctga ccttcttact gtgaatacca cctgatgtct
     1861 cagggaagtt tcaactggcc cattctccaa gcacttgagg tcacatcttg ttgtctgtgc
     1921 ctccagtggc aggcactact atccttccct ctctgcccat cccaagatac cagtcactga
     1981 cacgtgtagg ctgtagcctt agaaccaaca gcaggggatg cctcctagtc cagctccgct
     2041 gttcaaaccc atgttcacct agcagcctct gggaagagaa cagcactgtg ctgtcatgct
     2101 ggaatttagc agaatgcaat ttctttgcat caagaaattg actatgatat gacttgggga
     2161 agagggggag agtataagac acaatgtcat ggttgtctga atggagtcat gggctctatg
     2221 gaaacctgga cataatgttt ggcctgactc agattcgagc aaggaccaga cctctgggga
     2281 aatttcctag ctgtatctag tggaattgga ttagaatccc aataatcaaa tgggagtgca
     2341 ggcttgacac agccttgttg gcccaactgg aactgagttt atctccattg acaacctagc
     2401 tgcccacact ggaaacagca tgccatgagt cctccaccat cacctgctct tagggtcact
     2461 tttggtgacc ttacatttgg ccagaagcag tcagagaata gcagctggag agagagagag
     2521 agagagagag agagagagag agagagagag agagagagag agagcctttt cttccagttc
     2581 ttacagctgt gaaagtgtag acttgacaca aggagcataa gagagtgaag ttaggagcca
     2641 gtttgtgggg aaggggaaac ttgatcttca gtgtacgtag cactgtcaaa atacgatcct
     2701 gtgttcgaat ctgatcccct aagtacaacc acacccaggt caaactgttt ctggccttgg
     2761 acaagctctt agggcagatc aacactctgg gccctatacc ttcccaatcc ccagtatttt
     2821 catgaatgtg gatttctgac tgtgactgaa atcaatgcaa cacactggat cttggggggg
     2881 gggttagtaa actgaaggtt ctgagaacgt gggctctggg gtcagtaata aagacttcag
     2941 ggtgtgttta cttgacaaat gtcctgacct tccatgtaca cggagcaaat tcactccact
     3001 ggttcctctc acatactggg atgccagtcc ctgatctcca aagcaaagcc ctcctgtgtc
     3061 ttccaagatc tgccctcact caaacaggga aggttgctgt atttcttagt accattcatg
     3121 tagaggtgat gaccttacta ggagccaggc atatggcaaa aagcatatga aatacgaagg
     3181 atgatgcttt cttagcattt acatgagggt tcaatgagat gactatctta gggttttatt
     3241 gctgtgaaga gacaccatga ccatggcaac ttttataaag gaaaacattt cattg
//
