LOCUS       NM_008695               4913 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus nidogen 2 (Nid2), mRNA.
ACCESSION   NM_008695 NM_134085
VERSION     NM_008695.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Jacobson,K.R., Saleh,A.M., Lipp,S.N., Tian,C., Watson,A.R.,
            Luetkemeyer,C.M., Ocken,A.R., Spencer,S.L., Kinzer-Ursem,T.L. and
            Calve,S.
  TITLE     Extracellular matrix protein composition dynamically changes during
            murine forelimb development
  JOURNAL   iScience 27 (2), 108838 (2024)
   PUBMED   38303699
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Chen,Y., Mao,C., Gu,R., Zhao,R., Li,W., Ma,Z., Jia,Y., Yu,F.,
            Luo,J., Fu,Y., Sun,J. and Kong,W.
  TITLE     Nidogen-2 is a Novel Endogenous Ligand of LGR4 to Inhibit Vascular
            Calcification
  JOURNAL   Circ Res 131 (12), 1037-1054 (2022)
   PUBMED   36354004
  REMARK    GeneRIF: Nidogen-2 is a Novel Endogenous Ligand of LGR4 to Inhibit
            Vascular Calcification.
REFERENCE   3  (bases 1 to 4913)
  AUTHORS   Zhang,J.L., Richetti,S., Ramezani,T., Welcker,D., Lutke,S.,
            Pogoda,H.M., Hatzold,J., Zaucke,F., Keene,D.R., Bloch,W., Sengle,G.
            and Hammerschmidt,M.
  TITLE     Vertebrate extracellular matrix protein hemicentin-1 interacts
            physically and genetically with basement membrane protein nidogen-2
  JOURNAL   Matrix Biol 112, 132-154 (2022)
   PUBMED   36007682
REFERENCE   4  (bases 1 to 4913)
  AUTHORS   Naba,A., Clauser,K.R., Mani,D.R., Carr,S.A. and Hynes,R.O.
  TITLE     Quantitative proteomic profiling of the extracellular matrix of
            pancreatic islets during the angiogenic switch and insulinoma
            progression
  JOURNAL   Sci Rep 7, 40495 (2017)
   PUBMED   28071719
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4913)
  AUTHORS   Schminke,B., Frese,J., Bode,C., Goldring,M.B. and Miosge,N.
  TITLE     Laminins and Nidogens in the Pericellular Matrix of Chondrocytes:
            Their Role in Osteoarthritis and Chondrogenic Differentiation
  JOURNAL   Am J Pathol 186 (2), 410-418 (2016)
   PUBMED   26683663
REFERENCE   6  (bases 1 to 4913)
  AUTHORS   Sasaki,T., Gohring,W., Mann,K., Brakebusch,C., Yamada,Y.,
            Fassler,R. and Timpl,R.
  TITLE     Short arm region of laminin-5 gamma2 chain: structure, mechanism of
            processing and binding to heparin and proteins
  JOURNAL   J Mol Biol 314 (4), 751-763 (2001)
   PUBMED   11733994
REFERENCE   7  (bases 1 to 4913)
  AUTHORS   Neidhardt,L., Gasca,S., Wertz,K., Obermayr,F., Worpenberg,S.,
            Lehrach,H. and Herrmann,B.G.
  TITLE     Large-scale screen for genes controlling mammalian embryogenesis,
            using high-throughput gene expression analysis in mouse embryos
  JOURNAL   Mech Dev 98 (1-2), 77-94 (2000)
   PUBMED   11044609
REFERENCE   8  (bases 1 to 4913)
  AUTHORS   Murshed,M., Smyth,N., Miosge,N., Karolat,J., Krieg,T., Paulsson,M.
            and Nischt,R.
  TITLE     The absence of nidogen 1 does not affect murine basement membrane
            formation
  JOURNAL   Mol Cell Biol 20 (18), 7007-7012 (2000)
   PUBMED   10958695
REFERENCE   9  (bases 1 to 4913)
  AUTHORS   Talts,J.F., Andac,Z., Gohring,W., Brancaccio,A. and Timpl,R.
  TITLE     Binding of the G domains of laminin alpha1 and alpha2 chains and
            perlecan to heparin, sulfatides, alpha-dystroglycan and several
            extracellular matrix proteins
  JOURNAL   EMBO J 18 (4), 863-870 (1999)
   PUBMED   10022829
REFERENCE   10 (bases 1 to 4913)
  AUTHORS   Kimura,N., Toyoshima,T., Kojima,T. and Shimane,M.
  TITLE     Entactin-2: a new member of basement membrane protein with high
            homology to entactin/nidogen
  JOURNAL   Exp Cell Res 241 (1), 36-45 (1998)
   PUBMED   9633511
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY143483.1, AK164258.1, AK052839.1 and AY074820.1.
            
            On Jan 5, 2006 this sequence version replaced NM_008695.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054746.1, AK140832.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BY143483.1         1-30
            31-1977             AK164258.1         2-1948
            1978-3887           AK052839.1         1952-3861
            3888-4913           AY074820.1         3849-4874
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 10.73 cM"
     gene            1..4913
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /note="nidogen 2"
                     /db_xref="GeneID:18074"
                     /db_xref="MGI:MGI:1298229"
     exon            1..309
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..4293
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /note="entactin 2"
                     /codon_start=1
                     /product="nidogen-2 precursor"
                     /protein_id="NP_032721.2"
                     /db_xref="CCDS:CCDS36813.1"
                     /db_xref="GeneID:18074"
                     /db_xref="MGI:MGI:1298229"
                     /translation="MFRDPTAGWLTPPSPLSLLVMLLLLSRVGALRPDELFPYGESWG
                     DQLLPEGDDESSAAVKLAIPLRFYDAQFSSLYVGTNGIISTQDFPRETQYVDDDFPTD
                     FPAIAPFLADIDTSHSRGRILYREDTSGAVLSLAARYVRTGFPLSGSSFTPTHAFLAT
                     WEHVGAYEEVSRGAAPSGELNTFQAVLASDESDTYALFLYPANGLQFFGTRPKESYNV
                     QLQLPARVGFCRGEADDLKREALYFSLTNTEQSVKNLYQLSNLGIPGVWAFHIGSRFA
                     LDNVRPATVGGDPSTARSSALEHPFSHAAALESYTEDSFHYYDENEEDVEYPPVEPGE
                     APEGHSRIDVSFNSKADPGLVDVGTSSPGSDRASPWPYPAPGNWPSYRETESASLDPQ
                     TKQGRPVGEGEVLDFRDPAELLDQMGTRAPAPPEADAALLTPVNEDLGGRNTQSYPEA
                     GPVPSEPDVPVPPLEGEVLPHYPESGHVPPLRGGKYVIGLEDHVGSNDQVFTYNGANL
                     ETCEHSHGRCSQHAFCTDYTTGFCCHCQSRFYGNGKHCLPEGAPHRVNGKVSGRLRVG
                     HIPVHFTDVDLHAYIVGNDGRAYTAISHVPQPAAQALLPVLPIGGLFGWLFALEKPGS
                     ENGFSLTGATFVHDVEVTFHPGEERVRITQTAEGLDPENYLSIKTNIEGQVPFIPANF
                     TAHITPYKEFYHYRDSVVTSSSSRSFSLTSGSINQTWSYHIDQNITYQACRHAPRHLA
                     IPATQQLTVDRAFALYSEDEGVLRFAVTNQIGPVEVDSAPVGVNPCYDGSHTCDTTAR
                     CHPGTGVDYTCECTPGFQGDGRSCVDVNECATGFHRCGPNSVCVNLVGSYRCECRSGY
                     EFADDQHTCILIAPPPNPCLDGSHTCAPEGQARCIHHGGSSFSCACLPGFIGTGHQCS
                     DVDECAENRCHEAAICYNTPGSFSCRCQPGYRGDGFHCTSDTVPEDSISGLKPCEYQQ
                     RYAQTQHAYPGSRIHIPQCDDQGNFVPLQCHGSTGFCWCVDRNGHEVPGTQTPPGSTP
                     PHCGPPPEPTQRPRTVCERWRESLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDF
                     CWCVDKDGRELQGTRSQPGTRPACIPTVAPPVVRPTPRPDVTPPSVGTFLLYAQGQQI
                     GHLPLNGSRLQKDAARTLLSLHGSIVVGIDYDCRERMVYWTDVAGRTISRASLEAGAE
                     PETIITSGLISPEGLAIDHFRRTMYWTDSGLDKIERAELDGSERKVLFHTDLVNPRAI
                     TVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINKDIGLPNGLTFDPFSKLLCWAD
                     AGTKKLECTLPDGTGRRVIQNHLNYPFSIVSYADHFYHTDWRRDGVISVNKDSGQFTD
                     EFLPEQRSHLYGITAVYPYCPTGRK"
     exon            310..618
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            619..851
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1579
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1729
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1975
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2176
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2407
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2551
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2680
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2681..2824
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2825..2944
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..3193
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3194..3415
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3416..3585
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3586..3715
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3716..3887
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3888..4045
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4046..4169
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4170..4282
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4283..4884
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4864..4869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /note="hexamer: AATAAA"
     polyA_site      4884
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /note="major polyA site"
ORIGIN      
        1 agaggtccaa gttggagtct ggcgggcagt gtggcccgca gagacttgcg gagtggaggg
       61 cagagccagg gaggagtgag catgtttcgg gacccgacag ccgggtggct aacaccgcca
      121 tcaccgctat cgctgctggt gatgctgttg ctgctgtcgc gagtcggggc tctgcgtccc
      181 gacgagctct ttccttacgg ggagtcgtgg ggagaccagc tgctgccgga gggcgacgac
      241 gaaagctcag cagctgtgaa gctggcaata cccctgcgtt tctacgatgc ccagttcagc
      301 agcctctacg tgggcaccaa cggcatcatc tccacccagg acttccccag ggagactcaa
      361 tatgtagatg atgattttcc tactgacttc ccagccatcg cccctttcct ggctgacatc
      421 gacactagtc acagcagggg ccggatcctg taccgcgagg acacctccgg agctgtgctg
      481 agcctggctg cccgctacgt gcgcactggc ttcccgctgt cggggtccag cttcaccccc
      541 acccacgcct tcctggccac ctgggagcac gtgggcgcct acgaggaggt cagtcgcggg
      601 gctgcgccgt caggagagct gaacactttc caggctgttc tggcatccga tgaatctgat
      661 acctacgccc tctttctcta ccctgccaat ggccttcagt tctttggaac ccgccccaaa
      721 gagtcctaca atgtccagct gcagcttccc gctagggtgg gcttctgccg aggggaagcg
      781 gacgacctga agagagaagc actgtatttc agcctgacta acaccgagca gtctgtgaag
      841 aatctctacc agctaagcaa cctggggatt cctggagtgt gggcttttca catcggcagc
      901 aggtttgccc tggacaatgt ccgtccagcc acagttggag gtgacccttc cacagcccgt
      961 tcctcagcac tggagcaccc cttcagccat gctgcagccc tggagagcta caccgaggac
     1021 agtttccatt actacgatga gaatgaagag gatgttgagt acccaccagt tgaaccgggg
     1081 gaggccccgg aaggccacag cagaattgat gtgtctttca attcaaaggc tgatccaggc
     1141 cttgtggacg tcggcacctc atctccaggt tccgaccgcg cttctccttg gccatatcca
     1201 gcacctggta actggccatc ctaccgggaa acagaatcgg cttctttgga ccctcaaacc
     1261 aaacaggggc ggccggtggg agagggagag gttctggatt tcagggaccc agcggagctt
     1321 ttggaccaga tgggtaccag agccccagct cctccagagg cagatgcagc tctcctaact
     1381 ccagtcaacg aagaccttgg gggcagaaac acccagtcct atccagaggc agggccagtg
     1441 ccttcagagc cagatgttcc tgtccctcct ctggaaggag aagtccttcc tcattacccc
     1501 gagtctggtc acgttccgcc cctgagagga gggaagtatg tgataggact ggaggaccac
     1561 gtgggatcta atgatcaagt cttcacgtat aatggcgcca acctggaaac ctgcgaacac
     1621 agccacggcc ggtgctccca gcatgccttc tgcaccgact acaccactgg cttttgctgc
     1681 cactgccagt cgaggtttta cggaaacggg aagcactgcc tgccagaagg ggcacctcac
     1741 cgagtgaatg ggaaagtgag tggtcgcctc cgagtgggtc acattcctgt gcacttcact
     1801 gacgtggatc tacacgctta catcgtgggc aatgatggca gagcctacac tgccatcagc
     1861 catgtcccac agccggcagc ccaggccctg ctccctgtcc tgcccattgg aggtctgttt
     1921 ggctggctct ttgctttgga gaagccaggc tctgagaatg gcttcagcct cacaggtgct
     1981 acctttgtcc atgacgtgga agtcactttt caccctggag aggagagggt tcgtatcact
     2041 caaactgccg aggggcttga cccagaaaac tatctgagca ttaagaccaa cattgaaggc
     2101 caggtgccct tcatcccagc gaatttcaca gcccacatta ctccgtacaa agagttttat
     2161 cattacaggg actcagtcgt gacgtcctcc agttcccgaa gtttctctct cacttctggt
     2221 tctattaacc aaacgtggtc ctaccacatt gaccagaaca tcacttacca ggcgtgcagg
     2281 cacgccccca gacacctggc catccctgcc acccagcagc tgactgtcga ccgggccttt
     2341 gctttgtaca gtgaggatga gggcgtgctg aggtttgctg tgaccaatca gattggccct
     2401 gttgaagtgg actcagcccc tgttggagtg aatccttgct acgacggaag ccacacctgt
     2461 gacacaacag caaggtgcca cccgggaaca ggtgtagact acacctgcga gtgcacgcct
     2521 gggttccagg gtgacggacg gagctgtgtg gatgtcaatg agtgtgccac tggcttccac
     2581 cgctgtggcc ctaattctgt gtgtgtcaac ttggtgggaa gctacaggtg tgaatgccgc
     2641 agtggctatg aatttgcaga tgaccagcac acttgtatct tgatcgcccc acctcccaac
     2701 ccttgcctcg atggcagtca cacctgtgcc cctgaggggc aagccaggtg cattcaccat
     2761 ggaggcagct cattcagttg tgcctgcctg ccaggcttca ttggcactgg gcatcagtgt
     2821 tctgatgttg atgaatgtgc ggaaaaccga tgtcatgagg cagctatctg ctacaatacc
     2881 cccgggtcct tctcctgccg ttgccagcct gggtatcgag gggacgggtt tcactgcacc
     2941 tctgacacgg ttccggaaga ttccatctca ggactgaagc cctgtgaata ccagcagcgc
     3001 tatgctcaga cacagcacgc ctaccctggg tcacggatcc acatccccca gtgtgatgac
     3061 cagggaaact tcgtgccact gcagtgccac ggcagcactg gcttctgctg gtgtgtggac
     3121 cgaaatggcc acgaagtccc tggcacgcag actccacccg gctccacccc gccccactgt
     3181 ggaccacctc cagagcccac ccagaggcct cggactgtct gtgagcgttg gcgggaaagc
     3241 ctgctggaac actacggagg cacacccagg gatgaccagt atgtgcccca gtgtgatgac
     3301 ctgggccact tcatccccct gcagtgccat ggaaagagtg atttctgttg gtgtgtggac
     3361 aaggatggca gagagctaca gggcacgcgc tcacagccag gcaccaggcc tgcatgcata
     3421 cccaccgtcg ctccacccgt ggtccggccc acaccccggc ccgatgtgac tcctccatct
     3481 gtgggcacct tcctgctcta tgcccagggc caacagattg gccacttgcc cctcaatggc
     3541 agcaggcttc agaaggacgc agcccggacc ctgctgtcac tgcatggctc catagttgtg
     3601 gggattgact atgactgccg ggagaggatg gtctactgga cagacgttgc tggtcggacc
     3661 atcagccgtg ccagcttgga ggcaggagcc gaacccgaga ccatcattac ctcaggtttg
     3721 ataagcccgg aaggacttgc catcgaccac ttccgtagaa caatgtactg gacagatagt
     3781 ggcctggata agatagagcg ggccgaactg gatggttccg agcggaaagt cctcttccac
     3841 acagatctgg tgaatccacg agccatcact gtggatccaa tccgaggcaa cttgtactgg
     3901 acagactgga atagagaagc tcctaaaatt gaaacatcat ctttagatgg cgaaaacaga
     3961 aggattctga tcaacaaaga tattggatta cccaatggat tgacctttga ccccttctcc
     4021 aaactgctct gctgggcaga tgcaggaacc aaaaaactgg agtgtacact acccgatgga
     4081 actggacgac gtgtcatcca aaaccacctc aattacccct tcagcatcgt cagctatgca
     4141 gatcacttct accacacgga ctggcggagg gatggtgtta tatcagtgaa taaggacagt
     4201 ggccagttta ctgatgagtt tctccctgag cagcggtctc atctctatgg gatcactgca
     4261 gtctacccgt actgtccaac aggaagaaaa tgaacactaa atgtatagga ggatttcaag
     4321 tttacaagaa tcttgtccta agaatatttg ctacgaaggc aaagacagtg gaaaaggaat
     4381 tggccattat gagctcttgg cacacaagtg agcattttca gtgcagtgag actcaagtat
     4441 attttgtgaa atgtatacct caatctttac tactgtattt ttaaaaacaa aggttatcac
     4501 aagtttaaaa tagaatttta atcattgctt attaaatcaa cttttgtaaa cagttattta
     4561 aaaggccaaa ttcattcaac atagaaccaa tgagagcaaa gacactaaat ctgatttttg
     4621 tttgccatgg attctacctg gccaagaaaa gtaaagcaaa cagatggcta tgagaactga
     4681 ttcctgaagc ccactgaggc ccgccacaga cggaccatgg ggttggccca gcgaagtcct
     4741 cagactttat ttatactaag gtgctacaaa gagtccccaa gtgcctctac agaagtatcc
     4801 ctcaaaagta ctccgggtaa aacaccaaag atgcagcagt tcttcaattt tctatataat
     4861 tccaataaaa tattttaaag ctttaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//
