LOCUS       NP_032721               1403 aa            linear   ROD 02-JUN-2024
DEFINITION  nidogen-2 precursor [Mus musculus].
ACCESSION   NP_032721 NP_598846
VERSION     NP_032721.2
DBSOURCE    REFSEQ: accession NM_008695.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1403)
  AUTHORS   Jacobson,K.R., Saleh,A.M., Lipp,S.N., Tian,C., Watson,A.R.,
            Luetkemeyer,C.M., Ocken,A.R., Spencer,S.L., Kinzer-Ursem,T.L. and
            Calve,S.
  TITLE     Extracellular matrix protein composition dynamically changes during
            murine forelimb development
  JOURNAL   iScience 27 (2), 108838 (2024)
   PUBMED   38303699
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1403)
  AUTHORS   Chen,Y., Mao,C., Gu,R., Zhao,R., Li,W., Ma,Z., Jia,Y., Yu,F.,
            Luo,J., Fu,Y., Sun,J. and Kong,W.
  TITLE     Nidogen-2 is a Novel Endogenous Ligand of LGR4 to Inhibit Vascular
            Calcification
  JOURNAL   Circ Res 131 (12), 1037-1054 (2022)
   PUBMED   36354004
  REMARK    GeneRIF: Nidogen-2 is a Novel Endogenous Ligand of LGR4 to Inhibit
            Vascular Calcification.
REFERENCE   3  (residues 1 to 1403)
  AUTHORS   Zhang,J.L., Richetti,S., Ramezani,T., Welcker,D., Lutke,S.,
            Pogoda,H.M., Hatzold,J., Zaucke,F., Keene,D.R., Bloch,W., Sengle,G.
            and Hammerschmidt,M.
  TITLE     Vertebrate extracellular matrix protein hemicentin-1 interacts
            physically and genetically with basement membrane protein nidogen-2
  JOURNAL   Matrix Biol 112, 132-154 (2022)
   PUBMED   36007682
REFERENCE   4  (residues 1 to 1403)
  AUTHORS   Naba,A., Clauser,K.R., Mani,D.R., Carr,S.A. and Hynes,R.O.
  TITLE     Quantitative proteomic profiling of the extracellular matrix of
            pancreatic islets during the angiogenic switch and insulinoma
            progression
  JOURNAL   Sci Rep 7, 40495 (2017)
   PUBMED   28071719
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 1403)
  AUTHORS   Schminke,B., Frese,J., Bode,C., Goldring,M.B. and Miosge,N.
  TITLE     Laminins and Nidogens in the Pericellular Matrix of Chondrocytes:
            Their Role in Osteoarthritis and Chondrogenic Differentiation
  JOURNAL   Am J Pathol 186 (2), 410-418 (2016)
   PUBMED   26683663
REFERENCE   6  (residues 1 to 1403)
  AUTHORS   Sasaki,T., Gohring,W., Mann,K., Brakebusch,C., Yamada,Y.,
            Fassler,R. and Timpl,R.
  TITLE     Short arm region of laminin-5 gamma2 chain: structure, mechanism of
            processing and binding to heparin and proteins
  JOURNAL   J Mol Biol 314 (4), 751-763 (2001)
   PUBMED   11733994
REFERENCE   7  (residues 1 to 1403)
  AUTHORS   Neidhardt,L., Gasca,S., Wertz,K., Obermayr,F., Worpenberg,S.,
            Lehrach,H. and Herrmann,B.G.
  TITLE     Large-scale screen for genes controlling mammalian embryogenesis,
            using high-throughput gene expression analysis in mouse embryos
  JOURNAL   Mech Dev 98 (1-2), 77-94 (2000)
   PUBMED   11044609
REFERENCE   8  (residues 1 to 1403)
  AUTHORS   Murshed,M., Smyth,N., Miosge,N., Karolat,J., Krieg,T., Paulsson,M.
            and Nischt,R.
  TITLE     The absence of nidogen 1 does not affect murine basement membrane
            formation
  JOURNAL   Mol Cell Biol 20 (18), 7007-7012 (2000)
   PUBMED   10958695
REFERENCE   9  (residues 1 to 1403)
  AUTHORS   Talts,J.F., Andac,Z., Gohring,W., Brancaccio,A. and Timpl,R.
  TITLE     Binding of the G domains of laminin alpha1 and alpha2 chains and
            perlecan to heparin, sulfatides, alpha-dystroglycan and several
            extracellular matrix proteins
  JOURNAL   EMBO J 18 (4), 863-870 (1999)
   PUBMED   10022829
REFERENCE   10 (residues 1 to 1403)
  AUTHORS   Kimura,N., Toyoshima,T., Kojima,T. and Shimane,M.
  TITLE     Entactin-2: a new member of basement membrane protein with high
            homology to entactin/nidogen
  JOURNAL   Exp Cell Res 241 (1), 36-45 (1998)
   PUBMED   9633511
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY143483.1, AK164258.1, AK052839.1 and AY074820.1.
            
            On Jan 5, 2006 this sequence version replaced NP_032721.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054746.1, AK140832.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 10.73 cM"
     Protein         1..1403
                     /product="nidogen-2 precursor"
                     /note="entactin 2"
                     /calculated_mol_wt=150679
     sig_peptide     1..30
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=3253
     mat_peptide     31..1403
                     /product="Nidogen-2. /id=PRO_0000007672"
                     /note="propagated from UniProtKB/Swiss-Prot (O88322.2)"
                     /calculated_mol_wt=150679
     Region          108..275
                     /region_name="NIDO"
                     /note="Extracellular domain of unknown function in nidogen
                     (entactin) and hypothetical proteins; smart00539"
                     /db_xref="CDD:214712"
     Region          323..403
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O88322.2)"
     Site            386
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (chondroitin sulfate) serine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O88322.2)"
     Site            475
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (chondroitin sulfate) serine.
                     /evidence=ECO:0000250|UniProtKB:Q14112; propagated from
                     UniProtKB/Swiss-Prot (O88322.2)"
     Region          511..546
                     /region_name="EGF_3"
                     /note="EGF domain; pfam12947"
                     /db_xref="CDD:463759"
     Region          548..780
                     /region_name="nidG2"
                     /note="Nidogen, G2 domain; Nidogen is an important
                     component of the basement membrane, an extracellular
                     sheet-like matrix. Nidogen is a multifunctional protein
                     that interacts with many other basement membrane proteins,
                     like collagen, perlecan, lamin, and has a...; cl00186"
                     /db_xref="CDD:469646"
     Site            order(554,578,580,587,589,591,755,760,764,766)
                     /site_type="other"
                     /note="collagen/perlecan interaction surface [polypeptide
                     binding]"
                     /db_xref="CDD:238158"
     Site            681
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:19349973; propagated from
                     UniProtKB/Swiss-Prot (O88322.2)"
     Site            716
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O88322.2)"
     Site            726
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O88322.2)"
     Region          786..822
                     /region_name="EGF_3"
                     /note="EGF domain; pfam12947"
                     /db_xref="CDD:463759"
     Region          824..866
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain; smart00179"
                     /db_xref="CDD:214542"
     Site            order(824,827,843)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          919..952
                     /region_name="EGF_3"
                     /note="EGF domain; pfam12947"
                     /db_xref="CDD:463759"
     Region          946..948
                     /region_name="Cell attachment site"
                     /note="propagated from UniProtKB/Swiss-Prot (O88322.2)"
     Region          967..1028
                     /region_name="TY"
                     /note="Thyroglobulin type I repeats.; The N-terminal
                     region of human thyroglobulin contains 11 type-1 repeats
                     TY repeats are proposed to be inhibitors of cysteine
                     proteases; cd00191"
                     /db_xref="CDD:238114"
     Site            order(972,986,1004,1008)
                     /site_type="active"
                     /note="protease interaction site [active]"
                     /db_xref="CDD:238114"
     Region          1021..1043
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O88322.2)"
     Region          1047..1112
                     /region_name="TY"
                     /note="Thyroglobulin type I repeats.; The N-terminal
                     region of human thyroglobulin contains 11 type-1 repeats
                     TY repeats are proposed to be inhibitors of cysteine
                     proteases; cd00191"
                     /db_xref="CDD:238114"
     Site            order(1051,1066,1084,1088)
                     /site_type="active"
                     /note="protease interaction site [active]"
                     /db_xref="CDD:238114"
     Site            1152
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O88322.2)"
     Region          1162..1200
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1182..1225
                     /region_name="LDL-receptor class B 1"
                     /note="propagated from UniProtKB/Swiss-Prot (O88322.2)"
     Region          1206..1248
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1226..1268
                     /region_name="LDL-receptor class B 2"
                     /note="propagated from UniProtKB/Swiss-Prot (O88322.2)"
     Region          1249..1293
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1269..1313
                     /region_name="LDL-receptor class B 3"
                     /note="propagated from UniProtKB/Swiss-Prot (O88322.2)"
     Region          1294..1336
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1314..1355
                     /region_name="LDL-receptor class B 4"
                     /note="propagated from UniProtKB/Swiss-Prot (O88322.2)"
     Site            1336
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0007744|PubMed:24129315; propagated from
                     UniProtKB/Swiss-Prot (O88322.2)"
     Region          1357..1401
                     /region_name="LDL-receptor class B 5"
                     /note="propagated from UniProtKB/Swiss-Prot (O88322.2)"
     CDS             1..1403
                     /gene="Nid2"
                     /gene_synonym="entactin-2; Ly111; NID-2; nidogen-2"
                     /coded_by="NM_008695.2:82..4293"
                     /db_xref="CCDS:CCDS36813.1"
                     /db_xref="GeneID:18074"
                     /db_xref="MGI:MGI:1298229"
ORIGIN      
        1 mfrdptagwl tppsplsllv mllllsrvga lrpdelfpyg eswgdqllpe gddessaavk
       61 laiplrfyda qfsslyvgtn giistqdfpr etqyvdddfp tdfpaiapfl adidtshsrg
      121 rilyredtsg avlslaaryv rtgfplsgss ftpthaflat wehvgayeev srgaapsgel
      181 ntfqavlasd esdtyalfly panglqffgt rpkesynvql qlparvgfcr geaddlkrea
      241 lyfsltnteq svknlyqlsn lgipgvwafh igsrfaldnv rpatvggdps tarssalehp
      301 fshaaalesy tedsfhyyde needveyppv epgeapeghs ridvsfnska dpglvdvgts
      361 spgsdraspw pypapgnwps yretesasld pqtkqgrpvg egevldfrdp aelldqmgtr
      421 apappeadaa lltpvnedlg grntqsypea gpvpsepdvp vpplegevlp hypesghvpp
      481 lrggkyvigl edhvgsndqv ftynganlet cehshgrcsq hafctdyttg fcchcqsrfy
      541 gngkhclpeg aphrvngkvs grlrvghipv hftdvdlhay ivgndgrayt aishvpqpaa
      601 qallpvlpig glfgwlfale kpgsengfsl tgatfvhdve vtfhpgeerv ritqtaegld
      661 penylsiktn iegqvpfipa nftahitpyk efyhyrdsvv tssssrsfsl tsgsinqtws
      721 yhidqnityq acrhaprhla ipatqqltvd rafalysede gvlrfavtnq igpvevdsap
      781 vgvnpcydgs htcdttarch pgtgvdytce ctpgfqgdgr scvdvnecat gfhrcgpnsv
      841 cvnlvgsyrc ecrsgyefad dqhtciliap ppnpcldgsh tcapegqarc ihhggssfsc
      901 aclpgfigtg hqcsdvdeca enrcheaaic yntpgsfscr cqpgyrgdgf hctsdtvped
      961 sisglkpcey qqryaqtqha ypgsrihipq cddqgnfvpl qchgstgfcw cvdrnghevp
     1021 gtqtppgstp phcgpppept qrprtvcerw resllehygg tprddqyvpq cddlghfipl
     1081 qchgksdfcw cvdkdgrelq gtrsqpgtrp aciptvappv vrptprpdvt ppsvgtflly
     1141 aqgqqighlp lngsrlqkda artllslhgs ivvgidydcr ermvywtdva grtisrasle
     1201 agaepetiit sglispegla idhfrrtmyw tdsgldkier aeldgserkv lfhtdlvnpr
     1261 aitvdpirgn lywtdwnrea pkietssldg enrrilinkd iglpngltfd pfskllcwad
     1321 agtkklectl pdgtgrrviq nhlnypfsiv syadhfyhtd wrrdgvisvn kdsgqftdef
     1381 lpeqrshlyg itavypycpt grk
//
