LOCUS       NM_001290526            5382 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus NHS actin remodeling regulator (Nhs), transcript
            variant 1, mRNA.
ACCESSION   NM_001290526 XM_006528764
VERSION     NM_001290526.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Gan,P., Patterson,M., Watanabe,H., Wang,K., Edmonds,R.A.,
            Reinholdt,L.G. and Sucov,H.M.
  TITLE     Allelic variants between mouse substrains BALB/cJ and BALB/cByJ
            influence mononuclear cardiomyocyte composition and cardiomyocyte
            nuclear ploidy
  JOURNAL   Sci Rep 10 (1), 7605 (2020)
   PUBMED   32371981
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5382)
  AUTHORS   Sharma,S., Koh,K.S., Collin,C., Dave,A., McMellon,A., Sugiyama,Y.,
            McAvoy,J.W., Voss,A.K., Gecz,J. and Craig,J.E.
  TITLE     NHS-A isoform of the NHS gene is a novel interactor of ZO-1
  JOURNAL   Exp Cell Res 315 (14), 2358-2372 (2009)
   PUBMED   19447104
  REMARK    GeneRIF: the NHS-A isoform of NHS is a novel interactor of ZO-1 and
            may have a role at tight junctions. This isoform is important in
            mammalian development especially of the organs in the head.
REFERENCE   4  (bases 1 to 5382)
  AUTHORS   Sharma,S., Ang,S.L., Shaw,M., Mackey,D.A., Gecz,J., McAvoy,J.W. and
            Craig,J.E.
  TITLE     Nance-Horan syndrome protein, NHS, associates with epithelial cell
            junctions
  JOURNAL   Hum Mol Genet 15 (12), 1972-1983 (2006)
   PUBMED   16675532
  REMARK    GeneRIF: During lens development, continuous expression of NHS that
            became restricted to the lens epithelium in pre- and postnatal lens
            was seen, consistent with the finding that the NHS-A isoform
            associates with the apical cell membrane in the lens epithelium.
REFERENCE   5  (bases 1 to 5382)
  AUTHORS   Huang,K.M., Wu,J., Duncan,M.K., Moy,C., Dutra,A., Favor,J., Da,T.
            and Stambolian,D.
  TITLE     Xcat, a novel mouse model for Nance-Horan syndrome inhibits
            expression of the cytoplasmic-targeted Nhs1 isoform
  JOURNAL   Hum Mol Genet 15 (2), 319-327 (2006)
   PUBMED   16357105
  REMARK    GeneRIF: The first exon of Nhs1 contains crucial information
            required for the proper expression and localization of Nhs1
            protein.
REFERENCE   6  (bases 1 to 5382)
  AUTHORS   Zhou,E., Favor,J., Silvers,W. and Stambolian,D.
  TITLE     Exclusion of three candidate genes, Grpr, Cxn33, and Pdha1, for the
            X-linked cataract gene on the distal region of the mouse chromosome
            X
  JOURNAL   Mamm Genome 6 (5), 357-359 (1995)
   PUBMED   7626887
REFERENCE   7  (bases 1 to 5382)
  AUTHORS   Stambolian,D., Favor,J., Silvers,W., Avner,P., Chapman,V. and
            Zhou,E.
  TITLE     Mapping of the X-linked cataract (Xcat) mutation, the gene
            implicated in the Nance Horan syndrome, on the mouse X chromosome
  JOURNAL   Genomics 22 (2), 377-380 (1994)
   PUBMED   7806224
REFERENCE   8  (bases 1 to 5382)
  AUTHORS   Favor,J. and Pretsch,W.
  TITLE     Genetic localization and phenotypic expression of X-linked cataract
            (Xcat) in Mus musculus
  JOURNAL   Genet Res 56 (2-3), 157-162 (1990)
   PUBMED   2272505
REFERENCE   9  (bases 1 to 5382)
  AUTHORS   Stambolian,D., Lewis,R.A., Buetow,K., Bond,A. and Nussbaum,R.
  TITLE     Nance-Horan syndrome: localization within the region Xp21.1-Xp22.3
            by linkage analysis
  JOURNAL   Am J Hum Genet 47 (1), 13-19 (1990)
   PUBMED   1971992
REFERENCE   10 (bases 1 to 5382)
  AUTHORS   Favor,J., Neuhauser-Klaus,A. and Ehling,U.H.
  TITLE     Radiation-induced forward and reverse specific locus mutations and
            dominant cataract mutations in treated strain BALB/c and DBA/2 male
            mice
  JOURNAL   Mutat Res 177 (1), 161-169 (1987)
   PUBMED   3821762
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC147632.1, AL732391.7 and BG805095.1.
            
            On Mar 14, 2014 this sequence version replaced XM_006528764.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC158016.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-896               BC147632.1         1-896
            897-959             AL732391.7         101924-101986       c
            960-5021            BC147632.1         897-4958
            5022-5382           BG805095.1         240-600
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 74.17 cM"
     gene            1..5382
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /note="NHS actin remodeling regulator"
                     /db_xref="GeneID:195727"
                     /db_xref="MGI:MGI:2684894"
     exon            1..609
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..4997
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Nance-Horan syndrome"
                     /codon_start=1
                     /product="actin remodeling regulator NHS isoform 1"
                     /protein_id="NP_001277455.1"
                     /db_xref="CCDS:CCDS72461.1"
                     /db_xref="GeneID:195727"
                     /db_xref="MGI:MGI:2684894"
                     /translation="MPFAKRIVEPQWLCRQRRPAPGPDEDTSGGSVEPPPPLQPPGRR
                     EEAEAPEPEEPPRVPPAPLPLPPLPPSLPAPAEQDQQPPSEAREAGEESVAGVSEAAS
                     APGETSEASAEAVLLMLDLCAVSNAALARVLRQLSDVARHACSLFQELESDIQLTHRR
                     VWALQGKLGSVQRVLSTLDPKQEAVPVSNLDIESKLSVYYRAPWHQQRNIFLPATRPP
                     CVEELHRHARQSLQALRREHRSRSDRREQRAAAPLPIAAPPLPAYPPAHSQRRREAKD
                     RHFLTFNSTRSPSPTECCHMAPWSRKSHSPEDEDTDAMLGQRPKNPIHNVPSTLDKQT
                     NWNKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRK
                     LRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRRSGTRDSECQTED
                     ILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTPVVSSRTRSRSLPREG
                     NRGGDAEPKVGAKPSAFEEGERFVGDHERTPNDCSEAPSSPSTQEHQPALGLACSQHL
                     HSPQQKLSERGRSRLSRMAADSGSCDISSNSDTFGSPVHCISTASVLLSSHMDQKEDH
                     QSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPNANEDASVFVTE
                     QYNDHLDKVRGHRTNSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCRQDFSPERP
                     KADSLGCPSFTSMGTYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGLKGNKTYV
                     CHYAGLGPENGQGVGVPPALPDCAWQEYLDHRRQGRPSISFRKPKAKPTPPKRSSSLR
                     KSDGNADISEKKEPKISSGQHLPHSSREMKLPLDFSNTPSRVENANLPAKLDSSWINQ
                     SEHAIKEPQLDTPDISPFKDEGAESTHYADLWLLNDLKTNDPYRSLSNSSTATGTTVI
                     ECIKSPESSESQTSQSESRATTPSLPSVDNEYKLASPEKLAGLASPSSGYSSQSETPT
                     SSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGALSLSKDFELPIIPPSHLDL
                     SALHVLNKPFHHRHPLHVFTHKQNTVGDMPRSNPAPSLAITPTVLKSVNLRSISKSEE
                     VRQKEGNNTDLPYLEENAATAASVASLSPSKARPHTAKKSISRQYSAEDTLLPFLDSS
                     VAEMGPEKHLEKNPNFDGKSHGDPETATSASSNLPDSNVMKEQIQMESELISENILSK
                     NCGFSTGFQRVSASRPSDLGSKMIQYGASPDGTVQQGQKAPSGVREEGGKPESVDGIT
                     LQANSSTRVTDISSQYKHQRVISRHHDKVPVTIRHESEMSTVNSFPEKCSEQENIASG
                     ISPQSASDNSRAEETQGSMDETSLKESSPSDDSITSPLSEDSQAGAEGVFVSPNKPRT
                     TEDLFAVIHRSKRKVLGRKDSGDMSVRSKSRVPLGSSSSSANSVTSPSSNVTAGTSQR
                     SPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPVLPKPPGEFTAES
                     PQSPDDTHQGTPGTEALSPLSPCSPRVNAEGFSSKNFATSASARVGRSRVPPVASSSR
                     YSVRCRLYNTPMQAISEGETENSDGSPHDDRSSQSST"
     exon            610..762
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /inference="alignment:Splign:2.1.0"
     exon            763..896
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /inference="alignment:Splign:2.1.0"
     exon            897..959
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1152
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1284
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..4263
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /inference="alignment:Splign:2.1.0"
     exon            4264..4390
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /inference="alignment:Splign:2.1.0"
     exon            4391..5382
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5328..5333
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /note="hexamer: AATAAA"
     polyA_site      5354
                     /gene="Nhs"
                     /gene_synonym="Gm389; Gm48; Nhs1; Xcat"
                     /note="major polyA site"
ORIGIN      
        1 gctcacaagg gtcgctcaga ggagaaggga aagtcgcggc ccggctgcgc gggatgcctt
       61 tcgccaagcg gatcgtggag ccgcagtggc tttgccggca gcggcgcccg gcgcccggcc
      121 cagatgagga cacgagtgga ggcagcgttg agccgccgcc gcccctgcag ccgcctggcc
      181 ggcgggaaga ggccgaggca ccggagccag aagagcctcc tcgtgtgccc cctgcgcctc
      241 tcccactgcc acctctgccg ccgtctttgc cggcgccggc cgagcaggat cagcagccac
      301 ccagcgaagc gcgggaggcc ggggaggaga gcgtggcggg ggtctcggag gcagcatcgg
      361 ccccaggcga gacgtccgag gcttcagccg aggctgtgct gctcatgtta gacctgtgcg
      421 cggtcagcaa cgctgcgctg gccagggtcc tccggcagct ctcagacgta gcccggcacg
      481 cgtgcagcct cttccaggag ctcgagagcg acatccagct tactcaccgc cgtgtctggg
      541 cgctgcaggg caagctcggt agcgtgcagc gcgtcctcag cacgttggac cccaagcagg
      601 aggcagtgcc tgtctccaac ttggacatcg agagcaagtt gagtgtatac taccgtgcac
      661 catggcacca acagcgaaac atcttcctcc cagcgaccag gccaccctgt gtagaagagc
      721 tgcaccgcca tgccagacag agtctgcaag cccttcgtag agaacaccgg agccggagtg
      781 atcgcagaga gcaaagagct gctgctcccc tccccatagc tgctccacca ctaccagcct
      841 accctccagc tcacagccag aggaggcgcg aggccaagga ccgccacttc ctaacgttta
      901 acagcacccg ttcgccctcc cccactgaat gttgccacat ggccccatgg agtagaaagt
      961 cccactctcc agaggatgaa gacacagatg ccatgctagg gcagaggccg aaaaacccaa
     1021 tccacaatgt tccctctaca ctggataagc agaccaactg gaacaaagca ctacctctcc
     1081 caactcctga ggagaagatg aaacaagatg cccaagtgat ttcttcttgc atcattccca
     1141 tcaatgtcac cggtgttggt tttgacagag aagcaagtat acgctgttct cttgttcatt
     1201 cacaatctgt gctacagagg agacgaaaac tgaggagaag gaaaacaatc tctgggattc
     1261 ccagaagagt tcaacaagaa atagattctg atgaatcacc agtggccagg gaaaggaatg
     1321 tgattgtgca cacaaaccca gacccctcca acactgtcaa taggaggtcc ggaaccaggg
     1381 actctgaatg tcaaactgaa gatattctga ttgctgcccc atccagaagg agaattagag
     1441 ctcaaagggg tcaaagcatc gcagcttccc tttctcattc agctggcaac atctctgccc
     1501 tggcagacaa aggcgatacc atgtttactc ctgtcgtgag cagccgtaca agatctcgga
     1561 gccttcctcg ggagggaaat agaggtggag atgctgaacc caaagtaggt gctaaaccct
     1621 cagcatttga agaaggagaa cgtttcgtgg gtgaccatga aagaacccct aatgactgta
     1681 gcgaagctcc aagcagccca agtacacagg aacaccagcc tgctttgggc ctagcatgtt
     1741 ctcaacatct tcacagccct cagcaaaagt taagtgagag agggagatca cgtctgtccc
     1801 gaatggcggc tgactctgga agctgtgaca tctcttccaa ctcggacacc tttggaagcc
     1861 ccgtccattg catttccaca gctagtgtcc tccttagcag ccacatggac cagaaagaag
     1921 accaccagtc atccagtggt aactggagtg ggagcagctc tacatgcccg tcacagacct
     1981 cagagacaat ccctcctgca gcttctcctc ccctcacggg ctcttctcac tgtgactcag
     2041 aattgtcact gaacactgct cctaatgcta atgaagatgc cagtgtcttt gtgacagaac
     2101 agtacaatga ccacttggat aaagtaagag gccaccggac aaactccttt acttccactg
     2161 ttgcagatct gctggatgac cccaacaaca gcaatacaag tgacagtgag tggaattatc
     2221 tacaccacca tcatgatgca tcttgccgcc aagattttag tcctgagcgt ccgaaggcag
     2281 atagcttggg atgcccaagt tttacaagca tgggcaccta tgacagcttt ctggaaaagt
     2341 ctccttcaga caaagcagat actagctctc atttctcagt ggacactgaa ggatactata
     2401 cctcgatgca ctttgactgt ggtctcaaag gtaataagac ctatgtctgt cactatgcag
     2461 gcctaggccc agagaatggt cagggtgtcg gggttcctcc tgctcttccg gactgtgcct
     2521 ggcaagaata cttagaccac aggaggcagg ggagaccaag catctctttc aggaaaccaa
     2581 aggcaaagcc aactccacct aaacgtagct catcattgag aaaatctgat ggaaatgcag
     2641 acatttctga gaagaaagaa ccaaagataa gcagtggtca gcacctgcct cacagttcca
     2701 gggaaatgaa gctgcctctt gatttctcca acacgccttc tcgtgtggaa aatgccaatc
     2761 tgcctgcaaa gctggactct tcttggataa accagagtga acatgctatt aaggaacctc
     2821 agttggacac tccagacatt tcaccattca aagatgaagg tgctgaatca acacactatg
     2881 cagacctctg gcttctgaat gacttgaaaa cgaatgatcc ttacagatcc ttatctaatt
     2941 ctagcactgc tacgggtacc acagtgattg aatgcatcaa atctccagag agttctgaat
     3001 cccaaacatc ccaatcagaa tcaagagcaa ccactccatc tcttccttca gttgacaatg
     3061 aatataaact ggcttcacca gaaaagctgg ctggcttagc atctccatca agtggctact
     3121 caagccagtc agaaacgcca acctcctctt tccctacagc tttcttttca ggaccattgt
     3181 ctcctggagg tagcaaaaga aagcctaaag tcccagaaag gaaatcctca ctacagcagc
     3241 catctttaaa agatggtgcc ctatcactga gtaaagattt tgaactacca attatacctc
     3301 cttctcatct tgacctaagt gctcttcatg ttttgaataa accattccac caccgtcacc
     3361 cactgcatgt ttttactcat aagcaaaaca cagtcggaga tatgccgaga tcaaatcctg
     3421 caccgtctct tgcaattaca ccaacagttc tcaagtctgt caacctgagg tccatcagta
     3481 agtctgaaga agttaggcaa aaggagggca ataatacaga tctcccctac ttagaagaga
     3541 atgctgccac agcggcttct gtggcctcct tatctccaag taaggcaagg ccacacacag
     3601 caaagaaatc aatatcacgc cagtattctg ctgaagatac cctcctgccc tttttagatt
     3661 cctctgtagc tgagatggga ccagagaagc atttggaaaa gaaccctaat tttgatggaa
     3721 agagtcatgg tgatccagaa actgcaacct cagctagcag caatcttcca gattcaaatg
     3781 tgatgaaaga acaaatacag atggaaagcg agcttatctc agaaaacata ctaagtaaga
     3841 actgtggatt ttcaacagga tttcagaggg tgtctgcttc ccgccccagt gatttgggta
     3901 gtaaaatgat acagtatgga gctagtccag atggcactgt acagcaagga cagaaggcac
     3961 cctctggagt cagggaagaa ggtggaaaac cagaatctgt ggatggaatc acacttcaag
     4021 ccaattcatc aactagagta acagacataa gcagtcaata taagcatcag cgtgttataa
     4081 gccgccacca tgacaaagtg cctgtgacta tccgccatga gtcagagatg tcaactgtaa
     4141 attcattccc tgaaaaatgt tctgaacagg aaaatattgc ttcaggtatt tcaccccaaa
     4201 gtgcctctga taacagcaga gcagaggaga cccaaggaag tatggacgag acttcattga
     4261 aagaatcctc accaagtgat gactccatca cttcaccact cagtgaggac tctcaagctg
     4321 gagctgaggg tgtgtttgtg tctccaaaca aacctcgaac cactgaggac ctatttgcag
     4381 tcattcacag atccaagagg aaagtacttg gaagaaaaga ttctggggat atgtctgtca
     4441 gaagcaaatc aagagttccc cttggtagca gtagcagcag tgccaattct gtcacttcac
     4501 ccagcagcaa tgtgacagct gggactagcc agaggtctcc tggtctcatc tacagaaatg
     4561 ccaaaaagtc caacacatcc aatgaagagt ttaagctgct gctcctcaag aaaggcagtc
     4621 gttcagattc tagttaccgc atgtctgcta ctgaaatcct gaagagcccg gtcctgccca
     4681 agcctcctgg ggagtttact gcagagtccc cacaaagccc cgatgatacc catcagggca
     4741 cacctgggac tgaagcactg tctcctctgt cgccgtgctc accacgagtc aatgcggaag
     4801 ggttctcctc gaagaacttt gcaacctcag catcagcacg ggttggacgc tcccgggtcc
     4861 cacctgtggc cagcagcagc cgctacagtg tccgctgccg gctgtacaat actccaatgc
     4921 aggccatctc tgagggagag actgaaaact ctgatgggag cccacatgat gaccgatcct
     4981 cccagagctc cacctagttt gcctgagcca agctgacctc agatgtccag ccccttatta
     5041 atgaaaggat tttgagacat catagaggaa ctggagaaaa ggcaacaggg tgtgggggtt
     5101 gcattgttgc ttggcaatga gcttctaaat atgtgttgaa gaagcagaaa gtgaatgagt
     5161 cattttttaa attaattttc atgttttaag tagcagttct gtctcttgtg gttttcttta
     5221 gcacagtttg atttgttcga tctcattcct tgtgataata gagaatacaa aagtatataa
     5281 ctttcttgaa acttagacaa ggttttccca gagctgccta ttccgaaaat aaagccctca
     5341 ctgtcatgat tctataagaa gatgaaatta ctctggaggt tt
//
