LOCUS       NM_023055               2118 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus NHERF family PDZ scaffold protein 2 (Nherf2),
            transcript variant A, mRNA.
ACCESSION   NM_023055
VERSION     NM_023055.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Luo,M., Liu,Y., Nikolovska,K., Riederer,B., Patrucco,E., Hofmann,F.
            and Seidler,U.
  TITLE     cGMP-dependent kinase 2, Na+/H+ exchanger NHE3, and PDZ-adaptor
            NHERF2 co-assemble in apical membrane microdomains
  JOURNAL   Acta Physiol (Oxf) 240 (4), e14125 (2024)
   PUBMED   38533975
  REMARK    GeneRIF: cGMP-dependent kinase 2, Na[+]/H[+] exchanger NHE3, and
            PDZ-adaptor NHERF2 co-assemble in apical membrane microdomains.
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2118)
  AUTHORS   Ahuja,N., Hiltabidle,M.S., Rajasekhar,H., Voss,S., Lu,S.Z.,
            Barlow,H.R., Cowdin,M.A., Daniel,E., Vaddaraju,V., Anandakumar,T.,
            Black,E., Cleaver,O. and Maynard,C.
  TITLE     Endothelial Cyp26b1 restrains murine heart valve growth during
            development
  JOURNAL   Dev Biol 486, 81-95 (2022)
   PUBMED   35364055
REFERENCE   4  (bases 1 to 2118)
  AUTHORS   van Eif,V.W.W., Stefanovic,S., van Duijvenboden,K., Bakker,M.,
            Wakker,V., de Gier-de Vries,C., Zaffran,S., Verkerk,A.O.,
            Boukens,B.J. and Christoffels,V.M.
  TITLE     Transcriptome analysis of mouse and human sinoatrial node cells
            reveals a conserved genetic program
  JOURNAL   Development 146 (8) (2019)
   PUBMED   30936179
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2118)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2118)
  AUTHORS   Palmada,M., Poppendieck,S., Embark,H.M., van de Graaf,S.F.,
            Boehmer,C., Bindels,R.J. and Lang,F.
  TITLE     Requirement of PDZ domains for the stimulation of the epithelial
            Ca2+ channel TRPV5 by the NHE regulating factor NHERF2 and the
            serum and glucocorticoid inducible kinase SGK1
  JOURNAL   Cell Physiol Biochem 15 (1-4), 175-182 (2005)
   PUBMED   15665527
  REMARK    GeneRIF: demonstration of the significance of SGK1 and NHERF2 as
            TRPV5 modulators which are likely to participate in the regulation
            of calcium homeostasis by 1,25(OH)2D3
REFERENCE   7  (bases 1 to 2118)
  AUTHORS   Gisler,S.M., Pribanic,S., Bacic,D., Forrer,P., Gantenbein,A.,
            Sabourin,L.A., Tsuji,A., Zhao,Z.S., Manser,E., Biber,J. and
            Murer,H.
  TITLE     PDZK1: I. a major scaffolder in brush borders of proximal tubular
            cells
  JOURNAL   Kidney Int 64 (5), 1733-1745 (2003)
   PUBMED   14531806
REFERENCE   8  (bases 1 to 2118)
  AUTHORS   Shenolikar,S., Voltz,J.W., Minkoff,C.M., Wade,J.B. and Weinman,E.J.
  TITLE     Targeted disruption of the mouse NHERF-1 gene promotes
            internalization of proximal tubule sodium-phosphate cotransporter
            type IIa and renal phosphate wasting
  JOURNAL   Proc Natl Acad Sci U S A 99 (17), 11470-11475 (2002)
   PUBMED   12169661
REFERENCE   9  (bases 1 to 2118)
  AUTHORS   Gisler,S.M., Stagljar,I., Traebert,M., Bacic,D., Biber,J. and
            Murer,H.
  TITLE     Interaction of the type IIa Na/Pi cotransporter with PDZ proteins
  JOURNAL   J Biol Chem 276 (12), 9206-9213 (2001)
   PUBMED   11099500
REFERENCE   10 (bases 1 to 2118)
  AUTHORS   Zhang,J., Coward,P., Xian,M. and Lau,Y.F.
  TITLE     In vitro binding and expression studies demonstrate a role for the
            mouse Sry Q-rich domain in sex determination
  JOURNAL   Int J Dev Biol 43 (3), 219-227 (1999)
   PUBMED   10410901
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK084801.1 and CJ194744.1.
            
            On Apr 2, 2009 this sequence version replaced NM_023055.1.
            
            Transcript Variant: This variant (A) encodes the longer isoform
            (A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK084801.1, AK039491.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2112              AK084801.1         2-2113
            2113-2118           CJ194744.1         362-367
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 12.43 cM"
     gene            1..2118
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /note="NHERF family PDZ scaffold protein 2"
                     /db_xref="GeneID:65962"
                     /db_xref="MGI:MGI:1890662"
     exon            1..368
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..1169
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /note="isoform A is encoded by transcript variant A;
                     Na(+)/H(+) exchange regulatory cofactor NHE-RF2;
                     SRY-interacting protein 1; tyrosine kinase activator
                     protein 1; NHE3 kinase A regulatory protein E3KARP;
                     sodium-hydrogen exchanger regulatory factor 2; solute
                     carrier family 9 (sodium/hydrogen exchanger), member 3
                     regulator 2"
                     /codon_start=1
                     /product="Na(+)/H(+) exchange regulatory cofactor NHE-RF2
                     isoform A"
                     /protein_id="NP_075542.2"
                     /db_xref="CCDS:CCDS28488.1"
                     /db_xref="GeneID:65962"
                     /db_xref="MGI:MGI:1890662"
                     /translation="MAALESLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSP
                     AEAAALRAGDRLVEVNGVNVEGETHHQVVQRIKAVEGQTQLLVVDKETDEELCRRQLT
                     CTEEMAHRGLPPAHNPWEPKPDWACSGSLGSDTGQKDVNGPPRELRPRLCHLRRGPQG
                     YGFNLHSDKSRPGQYIRSVDPGSPASHSGLRAQDRLIEVNGQNVEGLRHAEVVARIKA
                     QEDEARLLVVDPETDEHFKRLRVIPTEEHVEGPLPSPVTNGTSPAQLNGGSVCSSRSD
                     LPGSEKDNEDGSTWKRDPFQESGLHLSPTAAEAKEKARATRVNKRAPQMDWNRKREIF
                     SNF"
     exon            369..569
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /inference="alignment:Splign:2.1.0"
     exon            570..749
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /inference="alignment:Splign:2.1.0"
     exon            750..903
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /inference="alignment:Splign:2.1.0"
     exon            904..947
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1010
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..2118
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1605..1610
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /note="hexamer: AATAAA"
     polyA_site      1630
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /note="major polyA site"
     regulatory      2093..2098
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /note="hexamer: ATTAAA"
     polyA_site      2114
                     /gene="Nherf2"
                     /gene_synonym="0610011L07Rik; 1200011K07Rik;
                     2010007A20Rik; E3karp; NHERF-2; Octs2; Sip-1; Sip1;
                     Slc9a3r2; Sryip1; Tka-1"
                     /note="major polyA site"
ORIGIN      
        1 agagccgaag ccggagcccg aggcgacgcg agctgagcag gagccaccga ggccgctgcc
       61 tgctctcccg cccgcgcctc cgccgcctcc gcactccagc ctgcgggtgg tgtgagccgt
      121 ggccagtcgc ggccggccag ggggcagcgg gtgccatggc cgcgctggaa tcgctgcggc
      181 cccgcttgtg tcgcctggtg cgcggagagc aagggtacgg ctttcacctg catggcgaga
      241 aaggccgccg tggccagttc atccggcgcg tggagccagg ctccccagcc gaggcggccg
      301 cgctgcgcgc cggggaccgc ctggttgagg tcaacggcgt caacgtagag ggcgagacgc
      361 atcaccaggt ggtacagagg atcaaggctg tggaggggca gacccagctg cttgtggtgg
      421 acaaggagac cgatgaggag ctctgccggc ggcaactgac ctgcactgag gagatggccc
      481 atcgagggct tcccccagcc cacaacccct gggagccaaa gccggactgg gcatgctcgg
      541 gcagccttgg ctcggacact ggccagaagg atgtcaatgg gcccccaaga gagctgcgtc
      601 cccggctctg ccacttgcga aggggacccc agggctacgg gttcaacctg catagtgaca
      661 agtcccggcc tggccagtac atccgctctg tggaccctgg ctctcctgct tctcactctg
      721 gcctccgtgc ccaggatcgg ctcattgagg tgaatgggca gaacgtggag gggctgcgac
      781 atgccgaggt tgtcgccaga atcaaggcac aggaggacga ggcccggctg ctggtagtcg
      841 atcctgagac tgatgaacac ttcaagcggt tgagggtcat acccactgag gaacatgtag
      901 aaggtccact gccatcacca gtcacgaacg ggaccagtcc tgcccagctc aatggtggct
      961 ctgtgtgctc atcccgaagt gacctgccag gctcagagaa ggacaatgag gatggcagta
     1021 cctggaagcg agaccccttc caggaaagtg gcctccacct gagtcccaca gcagctgagg
     1081 ccaaggagaa ggctcgagct actcgggtca acaagcgggc accacagatg gactggaacc
     1141 ggaagcgaga gatcttcagc aacttctgag tccctctttg cctgtccagg ggttccaggg
     1201 tctcttcctg ctcggacctt gggcctctcc agagctcccc aagcctcagc agactggagg
     1261 gtggtcacac accacccaga aaccaaccca tgaccccatg aacccttgtc ttgtccactg
     1321 ggtgctgcgg tggtcatagc aagatcagag aggagagaaa cccagagaag agagagacag
     1381 agatagacag ccagccaaga cagacacgga gacagaaaca gtgagagagg gaggagctgt
     1441 gcgtggtggt ctgccaaggg cctctgccat cctgcctcgg gcctgctgac tgaaaggaat
     1501 tcatgttttc gctttttttc ctaaccggtc tccagctcca catgtttcta gttaatagtg
     1561 gagccccctc ccccaacccc ttgggactca ctctaaataa ttgcaataaa acacaacctt
     1621 tctctgcaaa ccctttctta cccaccctgt cagcagcgcc tttctgagca gagaccaggg
     1681 aacaggcagt cagggacctc agggccagct gtgcctggag gagctgtccc tctgccccat
     1741 ccctcacatg ggatggtccc taggccaggc ctctcttctc tgcctagcta cccctgtgct
     1801 acctcccaca tgctgggttc tggaatgcag agcctaggct ctctgagggg ccagaggatg
     1861 ggtgttccat gactcaagag ggcagcctcc ggacaagccc cttatcctgt tctgcagctc
     1921 cagggcctgg aatcgtgggt ctgtgtgagc cattctcagg tatgatcctg ggctcctggg
     1981 cctgtcccct gccttatgcc tacctggctc ctaccctcca cccttgactc cccaaactca
     2041 ctcactggca ctgcagtctg cctcataagc catagcgcac gcgcgccctc aaattaaaca
     2101 ggccttgcct tgaactct
//
