LOCUS       NM_031375               1321 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus neugrin, neurite outgrowth associated (Ngrn),
            transcript variant 2, mRNA.
ACCESSION   NM_031375
VERSION     NM_031375.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1321)
  AUTHORS   Ishigaki,S., Niwa,J., Yoshihara,T., Mitsuma,N., Doyu,M. and
            Sobue,G.
  TITLE     Two novel genes, human neugrin and mouse m-neugrin, are upregulated
            with neuronal differentiation in neuroblastoma cells
  JOURNAL   Biochem Biophys Res Commun 279 (2), 526-533 (2000)
   PUBMED   11118320
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC109232.17, CJ154012.1, AK083330.1 and AI506588.1.
            
            On Jul 16, 2009 this sequence version replaced NM_031375.3.
            
            Transcript Variant: This variant (2) encodes the functional
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK144756.1, BB866631.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC109232.17        163439-163440
            3-367               CJ154012.1         2-366
            368-1317            AK083330.1         761-1710
            1318-1321           AI506588.1         1-4                 c
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 45.58 cM"
     gene            1..1321
                     /gene="Ngrn"
                     /note="neugrin, neurite outgrowth associated"
                     /db_xref="GeneID:83485"
                     /db_xref="MGI:MGI:1933212"
     exon            1..209
                     /gene="Ngrn"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..927
                     /gene="Ngrn"
                     /note="FI58Gm; m-Neugrin; neurite outgrowth-associated
                     protein"
                     /codon_start=1
                     /product="neugrin precursor"
                     /protein_id="NP_113552.3"
                     /db_xref="CCDS:CCDS21393.2"
                     /db_xref="GeneID:83485"
                     /db_xref="MGI:MGI:1933212"
                     /translation="MALSLSLFLGGRVRTSLARCGFASQVMAGPGSVSCEPDPDSDWE
                     PEERELQEVESALKRQKKAMRFQKIRRQMEAPGAPPRTLTWEAMEQIRYLHKEFAESW
                     SVPRLAEGFDVSTDVIRRVLKSKFVPTLEQKLRQDQKVLKKAGFTREIGQLPVSEDTL
                     KALSAGRSVSGLLMAGDEVSSKSQNHSTALKVAKSHPHSTDAQKKREGRDKRIQVLEE
                     SLVPATTALGHQRELQKSATSDSEATGRAGSDTLPSAVLLEELKPGEPGDQSFSSKVV
                     QRGHDFFDSNGNFLYRI"
     exon            210..320
                     /gene="Ngrn"
                     /inference="alignment:Splign:2.1.0"
     exon            321..1321
                     /gene="Ngrn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1300..1305
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ngrn"
                     /note="hexamer: AATAAA"
     polyA_site      1318
                     /gene="Ngrn"
                     /note="major polyA site"
ORIGIN      
        1 ccggtgcttt ccggtcgcga gctgtagata ccacctcgcg tcgcaatggc gctctctctg
       61 agcctctttt tgggcggtcg tgtccgcacc tctctcgctc gctgtgggtt cgcgtcccag
      121 gtcatggcgg gcccgggttc tgtcagctgc gagccggacc ccgattccga ctgggagccg
      181 gaggagcggg agctccagga ggtggagagc gctttgaaac gtcagaaaaa agcaatgcga
      241 ttccagaaaa tacgaaggca aatggaggct ccaggtgcac cacccagaac actgacgtgg
      301 gaagccatgg agcagatccg gtatttacat aaggaatttg cagagtcctg gtcagttcca
      361 aggttggcag aaggctttga tgtcagcact gatgttatcc gaagggtttt aaaaagtaag
      421 tttgtaccca ctttagagca gaaactgagg caggaccaaa aggtcttgaa aaaagctggg
      481 tttacccgag agattggtca gctccccgtt tctgaagata ccttgaaggc gctcagtgca
      541 ggccgctctg tgtctggcct gctgatggca ggggatgaag tctcctctaa aagtcagaat
      601 cacagcacag ctttgaaagt ggcgaagtcg caccctcaca gcacagacgc acagaagaaa
      661 cgggaaggga gggataaaag aatccaagtc ttggaagaga gcctagtgcc tgccaccaca
      721 gccttgggtc atcagcgaga gcttcagaaa tccgccacca gtgactccga ggccaccgga
      781 agagctggca gtgatacgtt accaagtgct gtgctgctgg aagagctgaa gcccggggag
      841 ccgggtgacc agagcttcag cagcaaggta gtgcagagag ggcatgactt ctttgacagc
      901 aatggaaact tcctctacag aatttgagcc tacgactggc ttttggagat gtcttgacac
      961 agctggacag gtagacttga agctgccagc atttttgcac accattggcc actttggagt
     1021 aagagttggt gaggctagaa tgtgggtgga ggagctactt aggcagggtc agacttcctg
     1081 gagtaagccc catgggtccc ctccggatct aagcactcat tggtctttgt tgcttggtgg
     1141 tctctctgtt gagaacaaga attatatgta tcctgtggtg gatttttgtg atactatatt
     1201 gggagaaaat tgaaagattg cctcctgacc tcatttcctt tctaatcact ggacaatcta
     1261 ttcttgatct gtttaatcaa ttggtttcct tatgtatgaa ataaagtgaa atggagcagt
     1321 t
//
