LOCUS       NM_021504               2901 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Mus musculus N-glycanase 1 (Ngly1), transcript variant 1, mRNA.
ACCESSION   NM_021504
VERSION     NM_021504.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Nedomova,M., Haberecht-Muller,S., Moller,S., Venz,S.,
            Prochazkova,M., Prochazka,J., Sedlak,F., Chawengsaksophak,K.,
            Hammer,E., Kasparek,P., Adamek,M., Sedlacek,R., Konvalinka,J.,
            Kruger,E. and Grantz Saskova,K.
  TITLE     DDI2 protease controls embryonic development and inflammation via
            TCF11/NRF1
  JOURNAL   iScience 27 (10), 110893 (2024)
   PUBMED   39328932
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Hetz,R., Magaway,C., Everett,J., Li,L., Willard,B.B., Freeze,H.H.
            and He,P.
  TITLE     Comparative proteomics reveals elevated CCN2 in NGLY1-deficient
            cells
  JOURNAL   Biochem Biophys Res Commun 632, 165-172 (2022)
   PUBMED   36209585
  REMARK    GeneRIF: Comparative proteomics reveals elevated CCN2 in
            NGLY1-deficient cells.
REFERENCE   3  (bases 1 to 2901)
  AUTHORS   Yoshida,Y., Asahina,M., Murakami,A., Kawawaki,J., Yoshida,M.,
            Fujinawa,R., Iwai,K., Tozawa,R., Matsuda,N., Tanaka,K. and
            Suzuki,T.
  TITLE     Loss of peptide:N-glycanase causes proteasome dysfunction mediated
            by a sugar-recognizing ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 118 (27) (2021)
   PUBMED   34215698
  REMARK    GeneRIF: Loss of peptide:N-glycanase causes proteasome dysfunction
            mediated by a sugar-recognizing ubiquitin ligase.
REFERENCE   4  (bases 1 to 2901)
  AUTHORS   Asahina,M., Fujinawa,R., Fujihira,H., Masahara-Negishi,Y.,
            Andou,T., Tozawa,R. and Suzuki,T.
  TITLE     JF1/B6F1 Ngly1-/- mouse as an isogenic animal model of NGLY1
            deficiency
  JOURNAL   Proc Jpn Acad Ser B Phys Biol Sci 97 (2), 89-102 (2021)
   PUBMED   33563880
REFERENCE   5  (bases 1 to 2901)
  AUTHORS   Talsness,D.M., Owings,K.G., Coelho,E., Mercenne,G., Pleinis,J.M.,
            Partha,R., Hope,K.A., Zuberi,A.R., Clark,N.L., Lutz,C.M.,
            Rodan,A.R. and Chow,C.Y.
  TITLE     A Drosophila screen identifies NKCC1 as a modifier of NGLY1
            deficiency
  JOURNAL   Elife 9, e57831 (2020)
   PUBMED   33315011
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2901)
  AUTHORS   Suzuki,T., Kwofie,M.A. and Lennarz,W.J.
  TITLE     Ngly1, a mouse gene encoding a deglycosylating enzyme implicated in
            proteasomal degradation: expression, genomic organization, and
            chromosomal mapping
  JOURNAL   Biochem Biophys Res Commun 304 (2), 326-332 (2003)
   PUBMED   12711318
REFERENCE   7  (bases 1 to 2901)
  AUTHORS   Hirsch,C., Blom,D. and Ploegh,H.L.
  TITLE     A role for N-glycanase in the cytosolic turnover of glycoproteins
  JOURNAL   EMBO J 22 (5), 1036-1046 (2003)
   PUBMED   12606569
  REMARK    GeneRIF: Data show that both the yeast PNG1 enzyme and its
            mammalian homolog display N-glycanase activity towards intact
            glycoproteins.
REFERENCE   8  (bases 1 to 2901)
  AUTHORS   Park,H., Suzuki,T. and Lennarz,W.J.
  TITLE     Identification of proteins that interact with mammalian
            peptide:N-glycanase and implicate this hydrolase in the
            proteasome-dependent pathway for protein degradation
  JOURNAL   Proc Natl Acad Sci U S A 98 (20), 11163-11168 (2001)
   PUBMED   11562482
REFERENCE   9  (bases 1 to 2901)
  AUTHORS   Suzuki,T., Park,H., Hollingsworth,N.M., Sternglanz,R. and
            Lennarz,W.J.
  TITLE     PNG1, a yeast gene encoding a highly conserved peptide:N-glycanase
  JOURNAL   J Cell Biol 149 (5), 1039-1052 (2000)
   PUBMED   10831608
REFERENCE   10 (bases 1 to 2901)
  AUTHORS   Suzuki,T., Seko,A., Kitajima,K., Inoue,Y. and Inoue,S.
  TITLE     Purification and enzymatic properties of peptide:N-glycanase from
            C3H mouse-derived L-929 fibroblast cells. Possible widespread
            occurrence of post-translational remodification of proteins by
            N-deglycosylation
  JOURNAL   J Biol Chem 269 (26), 17611-17618 (1994)
   PUBMED   8021270
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK028248.1.
            
            On Apr 6, 2007 this sequence version replaced NM_021504.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028248.1, BC028961.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2901              AK028248.1         2-2902
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 7.08 cM"
     gene            1..2901
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /note="N-glycanase 1"
                     /db_xref="GeneID:59007"
                     /db_xref="MGI:MGI:1913276"
     exon            1..184
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..2009
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /EC_number="3.5.1.52"
                     /note="isoform 1 is encoded by transcript variant 1;
                     peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
                     amidase; peptide N-glycanase homolog"
                     /codon_start=1
                     /product="peptide-N(4)-(N-acetyl-beta-
                     glucosaminyl)asparagine amidase isoform 1"
                     /protein_id="NP_067479.2"
                     /db_xref="CCDS:CCDS26832.1"
                     /db_xref="GeneID:59007"
                     /db_xref="MGI:MGI:1913276"
                     /translation="MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILR
                     NPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIR
                     DLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAP
                     MVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGT
                     NVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVE
                     NHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHV
                     WTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKH
                     DEVMSRRTKVKEELLRETINGLNKQRQLSLSESRRKELLQRIIVELVEFISPKTPRPG
                     ELGGRVSGSLAWRVARGETGLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNN
                     INISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSI
                     RTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAW
                     QHTQLFRQSLNDSGENGLEIIITFNDL"
     exon            185..299
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            300..536
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            537..702
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            703..925
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1047
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1193
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1304
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1469
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1655
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1833
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..2901
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2488..2493
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /note="hexamer: AATAAA"
     polyA_site      2512
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /note="major polyA site"
     regulatory      2701..2706
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /note="hexamer: AATAAA"
     polyA_site      2724
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
ORIGIN      
        1 gcctgctcag tgcgggccgc ggtgcgcgag gtggcgaagg ccgcggcgca gccatggcgt
       61 cggccacact gggcagctcg tcgagctctg cgtccccggc cgtggccgag ctatgccaga
      121 acaccccgga gacctttctg gaggcctcca agctgctgct cacctacgcc gacaacatcc
      181 tgagaaaccc cagtgatgaa aaatacagat ccatccgtat tgggaacaca gcgttttcta
      241 ctagactctt gcctgtcaga ggagctgttg agtgtttatt tgaaatgggc tttgaagagg
      301 gagaaacgca tcttatcttt cctaaaaaag cttcagtgga acagctacag aaaatccgtg
      361 atctgattgc catagagaga agcagcagac tggacggatc cagtaagaag gtgcaattct
      421 ctcagcaccc ggctgctgcc aagcttcctc tggagcagtc tgaagatcct gctgggttaa
      481 tacggcactc aggaaaccaa acagggcagc tgccaagcct accctctgct ccaatggttg
      541 ttggtgattc aaccatctta aaagttcttc agtccaacat tcagcatgtg cagctctatg
      601 aaaaccctgt cctccaggag aaagcattga cttgtatccc agtcagtgaa ctaaaaagaa
      661 aagctcaaga aaagttattc agagctagaa agttagataa aggtactaat gtaagtgatg
      721 aggacttcct tctactggag ctcctacact ggtttaaaga ggagtttttt cgctgggtga
      781 ataacattgt gtgcagcaaa tgtggtggag agactagatc tagagatgag gcattgctgc
      841 ccaatgatga tgaactgaag tggggtgcaa agaatgtgga gaatcattac tgtgatgcct
      901 gccagttgag caaccgcttc ccaagatata acaaccccga gaaacttttg gaaacaagat
      961 gtggacgctg tggtgaatgg gccaattgtt ttactctgtg ctgccgtgca ttagggtttg
     1021 aagctcgata tgtgtgggat tacacagacc atgtttggac agaagtctat tctccatctc
     1081 agcagcggtg gctacactgt gatgcatgtg aggatgtctg tgacaaaccc ctcctttatg
     1141 aaataggatg gggcaagaag ctttcctata taatagcatt ctctaaagat gaggtagttg
     1201 atgtcacctg gcgatactct tgtaaacatg atgaggtgat gtccaggagg accaaggtta
     1261 aagaagagtt acttcgagaa actattaatg ggcttaataa gcagagacag ctatcattat
     1321 cagaaagcag aaggaaagaa cttctccaga ggataattgt ggagcttgtt gaatttatat
     1381 ctcctaaaac cccaagacct ggagaacttg gtggaagggt atccgggtca ttggcttgga
     1441 gagtggcccg aggtgagacg ggtctagaga gaaaggagat tctgttcatc ccttctgaaa
     1501 atgaaaagat ttctaagcag tttcacctcc gttatgatat tgtgagagac cgttacattc
     1561 gtgtctcaga caacaacata aacatttctg gatgggagaa tggtgtgtgg aaaatggaat
     1621 ccatattcag aaaagtcgag aaagactgga acatggttta tttggcccga aaagaaggat
     1681 catcttttgc ttatatttcc tggaagtttg aatgtgggtc agctggtcta aaagtagata
     1741 ctgtttccat ccgcacaagt agccaaagct ttgagtctgg atcagtgagg tggaaactgc
     1801 gatctgagac agctcaagtc aacctgctgg gagataaaaa tctacgttcc tataatgatt
     1861 tttctggtgc cacagaggtt acattagaag cagagttaag cagaggagat ggagacgttg
     1921 cttggcaaca tacccaactg tttagacaaa gcttaaatga cagtggagaa aatggcttgg
     1981 aaataattat aacgttcaat gacctctgaa agcctgaact tcagggaaat gctggctaca
     2041 atcaccgacc cgccatggcc tccagtagtg tattagctca gtgtgtatct agttggcagt
     2101 tcaccatcct gtgcaacagg tttcttttgc tggctgtaca tcatgtaatc ctcattaaag
     2161 tatatcttca tagtgtggac cataagaaat cttcaattaa atgctaactt ctgcctgtga
     2221 ttagaatttg gcataaaata tttggcataa agtaactgta tgaaattcta atctaaacac
     2281 tgttactaaa gttgtaagtg aaaatgatgg tattcatgat taaatgctgt tcatgaatgt
     2341 gatacaatct agggctacta gttacttaac attttctgat tagatttttc caaacattct
     2401 caattttgaa taactgtcaa agtcaagtat cttatgtcat atgatgcatg aaggttttta
     2461 tcattgtaaa tatctggaca gttaaaaaat aaaatggatt atttttgctt tatgcctttt
     2521 cagtgcttac cttgcctaaa aattggcttt cctgggtatg aggaatattg aaaactacct
     2581 cagtatatac tggtttttca gacatttatt actaacagtt tatttaaaga tactttgcaa
     2641 gttccataat gcatgggaac ttgggtattc tttacctttg aattataaga tacctcattg
     2701 aataaaatat tcttggcttt gaaactgtct tgtatgagaa gattaagtga tgtgactgaa
     2761 atctaaaacc attaagcaga aagcagatac tgtttgagac atgctgtgat atactataga
     2821 gtaggtcact atagtttaag ctgcgtgaat ctgtttctag ctataggatt taataccaaa
     2881 tcaataaaaa tactggaatc t
//
