LOCUS       NM_001362432            2528 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Mus musculus N-glycanase 1 (Ngly1), transcript variant 2, mRNA.
ACCESSION   NM_001362432
VERSION     NM_001362432.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Nedomova,M., Haberecht-Muller,S., Moller,S., Venz,S.,
            Prochazkova,M., Prochazka,J., Sedlak,F., Chawengsaksophak,K.,
            Hammer,E., Kasparek,P., Adamek,M., Sedlacek,R., Konvalinka,J.,
            Kruger,E. and Grantz Saskova,K.
  TITLE     DDI2 protease controls embryonic development and inflammation via
            TCF11/NRF1
  JOURNAL   iScience 27 (10), 110893 (2024)
   PUBMED   39328932
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Hetz,R., Magaway,C., Everett,J., Li,L., Willard,B.B., Freeze,H.H.
            and He,P.
  TITLE     Comparative proteomics reveals elevated CCN2 in NGLY1-deficient
            cells
  JOURNAL   Biochem Biophys Res Commun 632, 165-172 (2022)
   PUBMED   36209585
  REMARK    GeneRIF: Comparative proteomics reveals elevated CCN2 in
            NGLY1-deficient cells.
REFERENCE   3  (bases 1 to 2528)
  AUTHORS   Yoshida,Y., Asahina,M., Murakami,A., Kawawaki,J., Yoshida,M.,
            Fujinawa,R., Iwai,K., Tozawa,R., Matsuda,N., Tanaka,K. and
            Suzuki,T.
  TITLE     Loss of peptide:N-glycanase causes proteasome dysfunction mediated
            by a sugar-recognizing ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 118 (27) (2021)
   PUBMED   34215698
  REMARK    GeneRIF: Loss of peptide:N-glycanase causes proteasome dysfunction
            mediated by a sugar-recognizing ubiquitin ligase.
REFERENCE   4  (bases 1 to 2528)
  AUTHORS   Asahina,M., Fujinawa,R., Fujihira,H., Masahara-Negishi,Y.,
            Andou,T., Tozawa,R. and Suzuki,T.
  TITLE     JF1/B6F1 Ngly1-/- mouse as an isogenic animal model of NGLY1
            deficiency
  JOURNAL   Proc Jpn Acad Ser B Phys Biol Sci 97 (2), 89-102 (2021)
   PUBMED   33563880
REFERENCE   5  (bases 1 to 2528)
  AUTHORS   Talsness,D.M., Owings,K.G., Coelho,E., Mercenne,G., Pleinis,J.M.,
            Partha,R., Hope,K.A., Zuberi,A.R., Clark,N.L., Lutz,C.M.,
            Rodan,A.R. and Chow,C.Y.
  TITLE     A Drosophila screen identifies NKCC1 as a modifier of NGLY1
            deficiency
  JOURNAL   Elife 9, e57831 (2020)
   PUBMED   33315011
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2528)
  AUTHORS   Suzuki,T., Kwofie,M.A. and Lennarz,W.J.
  TITLE     Ngly1, a mouse gene encoding a deglycosylating enzyme implicated in
            proteasomal degradation: expression, genomic organization, and
            chromosomal mapping
  JOURNAL   Biochem Biophys Res Commun 304 (2), 326-332 (2003)
   PUBMED   12711318
REFERENCE   7  (bases 1 to 2528)
  AUTHORS   Hirsch,C., Blom,D. and Ploegh,H.L.
  TITLE     A role for N-glycanase in the cytosolic turnover of glycoproteins
  JOURNAL   EMBO J 22 (5), 1036-1046 (2003)
   PUBMED   12606569
  REMARK    GeneRIF: Data show that both the yeast PNG1 enzyme and its
            mammalian homolog display N-glycanase activity towards intact
            glycoproteins.
REFERENCE   8  (bases 1 to 2528)
  AUTHORS   Park,H., Suzuki,T. and Lennarz,W.J.
  TITLE     Identification of proteins that interact with mammalian
            peptide:N-glycanase and implicate this hydrolase in the
            proteasome-dependent pathway for protein degradation
  JOURNAL   Proc Natl Acad Sci U S A 98 (20), 11163-11168 (2001)
   PUBMED   11562482
REFERENCE   9  (bases 1 to 2528)
  AUTHORS   Suzuki,T., Park,H., Hollingsworth,N.M., Sternglanz,R. and
            Lennarz,W.J.
  TITLE     PNG1, a yeast gene encoding a highly conserved peptide:N-glycanase
  JOURNAL   J Cell Biol 149 (5), 1039-1052 (2000)
   PUBMED   10831608
REFERENCE   10 (bases 1 to 2528)
  AUTHORS   Suzuki,T., Seko,A., Kitajima,K., Inoue,Y. and Inoue,S.
  TITLE     Purification and enzymatic properties of peptide:N-glycanase from
            C3H mouse-derived L-929 fibroblast cells. Possible widespread
            occurrence of post-translational remodification of proteins by
            N-deglycosylation
  JOURNAL   J Biol Chem 269 (26), 17611-17618 (1994)
   PUBMED   8021270
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK148931.1, AC173482.1 and BC028961.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148931.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1467              AK148931.1         2-1468
            1468-1469           AC173482.1         199203-199204
            1470-2512           AK148931.1         1472-2514
            2513-2528           BC028961.1         2465-2480
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 7.08 cM"
     gene            1..2528
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /note="N-glycanase 1"
                     /db_xref="GeneID:59007"
                     /db_xref="MGI:MGI:1913276"
     exon            1..186
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..2011
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /EC_number="3.5.1.52"
                     /note="isoform 2 is encoded by transcript variant 2;
                     peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine
                     amidase; peptide N-glycanase homolog"
                     /codon_start=1
                     /product="peptide-N(4)-(N-acetyl-beta-
                     glucosaminyl)asparagine amidase isoform 2"
                     /protein_id="NP_001349361.1"
                     /db_xref="GeneID:59007"
                     /db_xref="MGI:MGI:1913276"
                     /translation="MPEHPGDLSGGLQAAAHLRRQHPEVNPSDEKYRSIRIGNTAFST
                     RLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQ
                     FSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHV
                     QLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEE
                     FFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNNP
                     EKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACE
                     DVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETI
                     NGLNKQRQLSLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGET
                     GLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRK
                     VEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSE
                     TAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGLE
                     IIITFNDL"
     exon            187..301
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            302..538
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            539..704
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            705..927
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1049
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1195
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1306
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1471
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1657
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1835
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..2514
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2490..2495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /note="hexamer: AATAAA"
     polyA_site      2514
                     /gene="Ngly1"
                     /gene_synonym="1110002C09Rik; Png1; PNGase"
                     /note="major polyA site"
ORIGIN      
        1 gcctgctcag tgcgggccgc ggtgcgcgag gtggcgaagg ccgcggcgca gccatggcgt
       61 cggccacact gggcagctcg tcgagctctg cgtccccggc cgtggccgag ctatgccaga
      121 acaccccgga gacctttctg gaggcctcca agctgctgct cacctacgcc gacaacatcc
      181 tgaggtaaac cccagtgatg aaaaatacag atccatccgt attgggaaca cagcgttttc
      241 tactagactc ttgcctgtca gaggagctgt tgagtgttta tttgaaatgg gctttgaaga
      301 gggagaaacg catcttatct ttcctaaaaa agcttcagtg gaacagctac agaaaatccg
      361 tgatctgatt gccatagaga gaagcagcag actggacgga tccagtaaga aggtgcaatt
      421 ctctcagcac ccggctgctg ccaagcttcc tctggagcag tctgaagatc ctgctgggtt
      481 aatacggcac tcaggaaacc aaacagggca gctgccaagc ctaccctctg ctccaatggt
      541 tgttggtgat tcaaccatct taaaagttct tcagtccaac attcagcatg tgcagctcta
      601 tgaaaaccct gtcctccagg agaaagcatt gacttgtatc ccagtcagtg aactaaaaag
      661 aaaagctcaa gaaaagttat tcagagctag aaagttagat aaaggtacta atgtaagtga
      721 tgaggacttc cttctactgg agctcctaca ctggtttaaa gaggagtttt ttcgctgggt
      781 gaataacatt gtgtgcagca aatgtggtgg agagactaga tctagagatg aggcattgct
      841 gcccaatgat gatgaactga agtggggtgc aaagaatgtg gagaatcatt actgtgatgc
      901 ctgccagttg agcaaccgct tcccaagata taacaacccc gagaaacttt tggaaacaag
      961 atgtggacgc tgtggtgaat gggccaattg ttttactctg tgctgccgtg cattagggtt
     1021 tgaagctcga tatgtgtggg attacacaga ccatgtttgg acagaagtct attctccatc
     1081 tcagcagcgg tggctacact gtgatgcatg tgaggatgtc tgtgacaaac ccctccttta
     1141 tgaaatagga tggggcaaga agctttccta tataatagca ttctctaaag atgaggtagt
     1201 tgatgtcacc tggcgatact cttgtaaaca tgatgaggtg atgtccagga ggaccaaggt
     1261 taaagaagag ttacttcgag aaactattaa tgggcttaat aagcagagac agctatcatt
     1321 atcagaaagc agaaggaaag aacttctcca gaggataatt gtggagcttg ttgaatttat
     1381 atctcctaaa accccaagac ctggagaact tggtggaagg gtatccgggt cattggcttg
     1441 gagagtggcc cgaggtgaga cgggtctaga gagaaaggag attctgttca tcccttctga
     1501 aaatgaaaag atttctaagc agtttcacct ccgttatgat attgtgagag accgttacat
     1561 tcgtgtctca gacaacaaca taaacatttc tggatgggag aatggtgtgt ggaaaatgga
     1621 atccatattc agaaaagtcg agaaagactg gaacatggtt tatttggccc gaaaagaagg
     1681 atcatctttt gcttatattt cctggaagtt tgaatgtggg tcagctggtc taaaagtaga
     1741 tactgtttcc atccgcacaa gtagccaaag ctttgagtct ggatcagtga ggtggaaact
     1801 gcgatctgag acagctcaag tcaacctgct gggagataaa aatctacgtt cctataatga
     1861 tttttctggt gccacagagg ttacattaga agcagagtta agcagaggag atggagacgt
     1921 tgcttggcaa catacccaac tgtttagaca aagcttaaat gacagtggag aaaatggctt
     1981 ggaaataatt ataacgttca atgacctctg aaagcctgaa cttcagggaa atgctggcta
     2041 caatcaccga cccgccatgg cctccagtag tgtattagct cagtgtgtat ctagttggca
     2101 gttcaccatc ctgtgcaaca ggtttctttt gctggctgta catcatgtaa tcctcattaa
     2161 agtatatctt catagtgtgg accataagaa atcttcaatt aaatgctaac ttctgcctgt
     2221 gattagaatt tggcataaaa tatttggcat aaagtaactg tatgaaattc taatctaaac
     2281 actgttacta aagttgtaag tgaaaatgat ggtattcatg attaaatgct gttcatgaat
     2341 gtgatacaat ctagggctac tagttactta acattttctg attagatttt tccaaacatt
     2401 ctcaattttg aataactgtc aaagtcaagt atcttatgtc atatgatgca tgaaggtttt
     2461 tatcattgta aatatctgga cagttaaaaa ataaaatgga ttatttttgc tttaaaaaaa
     2521 aaaaaaaa
//
