LOCUS       NM_019867               2764 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus neuronal guanine nucleotide exchange factor (Ngef),
            transcript variant 2, mRNA.
ACCESSION   NM_019867
VERSION     NM_019867.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ahuja,N., Hiltabidle,M.S., Rajasekhar,H., Voss,S., Lu,S.Z.,
            Barlow,H.R., Cowdin,M.A., Daniel,E., Vaddaraju,V., Anandakumar,T.,
            Black,E., Cleaver,O. and Maynard,C.
  TITLE     Endothelial Cyp26b1 restrains murine heart valve growth during
            development
  JOURNAL   Dev Biol 486, 81-95 (2022)
   PUBMED   35364055
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Yorgan,T.A., Peters,S., Amling,M. and Schinke,T.
  TITLE     Osteoblast-specific expression of Panx3 is dispensable for
            postnatal bone remodeling
  JOURNAL   Bone 127, 155-163 (2019)
   PUBMED   31202927
REFERENCE   3  (bases 1 to 2764)
  AUTHORS   Jung,H., Park,H., Choi,Y., Kang,H., Lee,E., Kweon,H., Roh,J.D.,
            Ellegood,J., Choi,W., Kang,J., Rhim,I., Choi,S.Y., Bae,M.,
            Kim,S.G., Lee,J., Chung,C., Yoo,T., Park,H., Kim,Y., Ha,S.,
            Um,S.M., Mo,S., Kwon,Y., Mah,W., Bae,Y.C., Kim,H., Lerch,J.P.,
            Paik,S.B. and Kim,E.
  TITLE     Sexually dimorphic behavior, neuronal activity, and gene expression
            in Chd8-mutant mice
  JOURNAL   Nat Neurosci 21 (9), 1218-1228 (2018)
   PUBMED   30104731
REFERENCE   4  (bases 1 to 2764)
  AUTHORS   Chang,C.J., Chang,M.Y., Chou,S.Y., Huang,C.C., Chuang,J.Y.,
            Hsu,T.I., Chang,H.F., Wu,Y.H., Wu,C.C., Morales,D., Kania,A. and
            Kao,T.J.
  TITLE     Ephexin1 Is Required for Eph-Mediated Limb Trajectory of Spinal
            Motor Axons
  JOURNAL   J Neurosci 38 (8), 2043-2056 (2018)
   PUBMED   29363583
  REMARK    GeneRIF: We found ephexin1 essential for the development of limb
            nerves toward their muscle targets, concluding that it functions as
            an intermediary to relay Eph signaling in this context
REFERENCE   5  (bases 1 to 2764)
  AUTHORS   Zhang,J.C., Yao,W., Qu,Y., Nakamura,M., Dong,C., Yang,C., Ren,Q.,
            Ma,M., Han,M., Shirayama,Y., Hayashi-Takagi,A. and Hashimoto,K.
  TITLE     Increased EphA4-ephexin1 signaling in the medial prefrontal cortex
            plays a role in depression-like phenotype
  JOURNAL   Sci Rep 7 (1), 7133 (2017)
   PUBMED   28769056
  REMARK    GeneRIF: These findings suggest that increased EphA4-ephexin1
            signaling in the PFC plays a role in the pathophysiology of
            depression.
            Erratum:[Sci Rep. 2023 May 17;13(1):8059. doi:
            10.1038/s41598-023-34971-6.. PMID: 37198278]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2764)
  AUTHORS   Funatsu,N., Inoue,T. and Nakamura,S.
  TITLE     Gene expression analysis of the late embryonic mouse cerebral
            cortex using DNA microarray: identification of several region- and
            layer-specific genes
  JOURNAL   Cereb Cortex 14 (9), 1031-1044 (2004)
   PUBMED   15142957
REFERENCE   7  (bases 1 to 2764)
  AUTHORS   Yoshizawa,M., Sone,M., Matsuo,N., Nagase,T., Ohara,O., Nabeshima,Y.
            and Hoshino,M.
  TITLE     Dynamic and coordinated expression profile of dbl-family guanine
            nucleotide exchange factors in the developing mouse brain
  JOURNAL   Gene Expr Patterns 3 (3), 375-381 (2003)
   PUBMED   12799088
REFERENCE   8  (bases 1 to 2764)
  AUTHORS   Shamah,S.M., Lin,M.Z., Goldberg,J.L., Estrach,S., Sahin,M., Hu,L.,
            Bazalakova,M., Neve,R.L., Corfas,G., Debant,A. and Greenberg,M.E.
  TITLE     EphA receptors regulate growth cone dynamics through the novel
            guanine nucleotide exchange factor ephexin
  JOURNAL   Cell 105 (2), 233-244 (2001)
   PUBMED   11336673
REFERENCE   9  (bases 1 to 2764)
  AUTHORS   Rodrigues,N.R., Theodosiou,A.M., Nesbit,M.A., Campbell,L.,
            Tandle,A.T., Saranath,D. and Davies,K.E.
  TITLE     Characterization of Ngef, a novel member of the Dbl family of genes
            expressed predominantly in the caudate nucleus
  JOURNAL   Genomics 65 (1), 53-61 (2000)
   PUBMED   10777665
REFERENCE   10 (bases 1 to 2764)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ149200.1, AK138433.1, AJ238898.1 and AI847102.1.
            
            On Dec 13, 2007 this sequence version replaced NM_019867.1.
            
            Transcript Variant: This variant (2) contains a different segment
            for its 5' UTR and 5' coding region, and lacks other coding region
            exons, compared to variant 1. The resulting protein (isoform 2) has
            a shorter and distinct N-terminus when it is compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY038025.1, AK138433.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                CJ149200.1         1-31
            32-413              AK138433.1         2-383
            414-2530            AJ238898.1         395-2511
            2531-2764           AI847102.1         1-234               c
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 44.42 cM"
     gene            1..2764
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /note="neuronal guanine nucleotide exchange factor"
                     /db_xref="GeneID:53972"
                     /db_xref="MGI:MGI:1858414"
     exon            1..270
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..2026
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ephexin-1; eph-interacting exchange protein; transforming
                     immortalised mammary epithelial sequence 2"
                     /codon_start=1
                     /product="ephexin-1 isoform 2"
                     /protein_id="NP_063920.2"
                     /db_xref="CCDS:CCDS48306.1"
                     /db_xref="GeneID:53972"
                     /db_xref="MGI:MGI:1858414"
                     /translation="MELLAAAFSAACAVDHDSSTSESDTRDSAAGHLPGSESSSTPGN
                     GTTPEECPALTDSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETR
                     RQQDAEIQGNSDGSQVGEDAGEEEEEEEEGEEEELASPPERRALPQICLLSNPHSRFN
                     LWQDLPEIQSSGVLDILQPEEIRLQEAMFELVTSEASYYKSLNLLVSHFMENERLKKI
                     LHPSEAHILFSNVLDVMAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITY
                     VSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCKGLPFSSFLILPFQRITRLKLLVQ
                     NILKRVEERSEREGTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVP
                     IISHSRWLLKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVF
                     DSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAP
                     NRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILEKTEDGWIFGERLH
                     DQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMEDPQRSQNKDRRKLGSRNRQ"
     exon            271..413
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            414..721
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            722..882
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1035
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1165
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1240
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1330
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1494
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1650
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1730
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1835
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..2751
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2722..2727
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /note="hexamer: ATTAAA"
     polyA_site      2746
                     /gene="Ngef"
                     /gene_synonym="ephexin; Tims2"
                     /note="major polyA site"
ORIGIN      
        1 gcgctcgtcc gcgggagcgc tctacagcag cggcggcggc agctccggct tgagccgcgc
       61 gcgctgcgac ctcactcaga gcccgcgcat tgcccccggc tgggccctgg gccccccgcg
      121 cggctcccca ccagcccctg agcctacccg gtcgctggtc cccatggagc tgctggctgc
      181 agccttcagc gccgcctgcg ccgtggacca cgacagctcc acctcggaga gcgacacgcg
      241 cgactcggcg gcgggacacc tgccgggcag cgagtcatcc tccacccctg gaaatggaac
      301 cacacccgag gagtgcccag ccctcaccga cagccccacc actctcacgg aggccctgca
      361 gatgatccat cccattcccg ccgactcctg gagaaacctc attgaacaaa tagggctcct
      421 gtatcaagag tatagagaca aatcgactct ccaagaaatt gaaacacgga ggcagcagga
      481 tgcagaaatc caaggcaact ccgatgggtc ccaggttggg gaagacgctg gagaggagga
      541 ggaggaggag gaggagggag aggaggagga gctggccagc cctcctgaga ggagagctct
      601 gcctcagatc tgcctgctca gcaaccccca ctccaggttc aacctctggc aagaccttcc
      661 tgagatccag agcagtggcg tgctggacat tctccagccg gaggagatca ggctgcagga
      721 ggccatgttt gagttggtta cctctgaggc ctcctactat aagagcctga acctgctggt
      781 gtcgcacttc atggagaacg agcgtctgaa gaagatcctg catccatctg aggcccacat
      841 cctcttttcc aatgtcctgg atgtcatggc tgtcagtgag cggtttttgc tggagctaga
      901 gcaccgcatg gaggagaaca ttgttatctc ggatgtgtgc gacatcgtgt accgttacgc
      961 agctgatcac ttctcggtct atatcactta cgtcagtaac cagacctacc aggaaaggac
     1021 atacaagcag ctcctacagg agaaggccgc tttccgggaa ctgatcgcac agttggagct
     1081 ggaccccaaa tgcaagggcc tgcctttctc ctccttcctc atcttgcctt tccagaggat
     1141 cacgagactc aagctgctgg tccagaatat cctgaagaga gtggaggaga ggtctgaacg
     1201 tgaaggcacc gccttggatg cccacaagga gctagaaatg gtggtaaagg catgcaatga
     1261 gggtgtccgg aagatgagcc gcacagaaca gatgatcagc attcagaaga agatggagtt
     1321 caagatcaag tcggtaccca tcatctcaca ctcccggtgg ctgctgaagc agggtgagct
     1381 gcagcagatg tccggcccca agacctcccg caccctgcgg accaagaagc tcttcagaga
     1441 aatttacctc ttcctcttca atgacctgct ggtgatctgc cggcagatcc ctggagacaa
     1501 gtaccaggtg tttgattcgg ccccaagggg cctgcttcga gtggaggagc tggaggacca
     1561 gggtcaaaca ctggctaatg tgttcatcct gcggctgctg gaaaatgcag atgaccgaga
     1621 ggccacctat atgctgaagg catcctccca gagcgagatg aagcgctgga tgacctcact
     1681 ggcccccaac aggaggacca agtttgtatc cttcacatct cggctgttgg actgtcccca
     1741 ggtccagtgt gtgcacccgt atgtggccca gcagcctgat gaactgacgc tggaactggc
     1801 agatatcctg aacatcctgg agaagacaga ggatgggtgg atctttggtg agcggctgca
     1861 tgaccaggag agaggctggt tccccagttc catgacagag gagatcctga accccaagat
     1921 ccgctcccag aacctcaagg aatgtttccg ggtacataag atggaagacc ctcagcgcag
     1981 ccagaataag gaccgcagga agctgggcag ccggaatcgt caatgaacct ccccagctca
     2041 ggcacctgaa gggaagggtg tgggcaggga tggggagcag gcccggcaga gacgcccgac
     2101 agattcagag ggccttaggg aagaatgtca gtgccttctc aggcagcagg agtggcttcg
     2161 gcctgctctg tccctgccca tgctgtggaa gctctagtgt cctggccact tgtttgcttg
     2221 cacactggtg aaaagctaag tacttaggca gtattacacc acctcccttc agtctctcag
     2281 aggtagaaga aggcaggcat gctccagaga ccttccggtg actggaagag gcccacacaa
     2341 gggtccctgg cagcaggcag gtggaaggta accactgtca ggatcccctg aactgcacgt
     2401 gtccttccct actttggaag ctgttaagag tctaccaggc acacagatgg ccgcccctgc
     2461 ccgagggagt ttgatgagca gtggtgaccc tgcctgcccg tccccgtgcc tctgccagcc
     2521 tctcttgcac ggccaagccc tgccctcagc aggcttccca aagcttagct gagggttcat
     2581 gccacctcta gctccttgaa gggcttgata tcacttgtgt ctcctggccc ctgatggagc
     2641 ccaggcgttt tgcagaatga attggtcact gcatccttta tggtcatggt tttgagaaaa
     2701 gcaaatatca tttttggctg cattaaaaga agcatcctat ataaaacaag aaaaaaaaaa
     2761 aaaa
//
